Restriction map for
pF1KB1290
(Circular) (Six-base) MAPSORT of: pF1KB1290.seq Check: 1515 from: 1 to: 5360
pF1KB1290 5360 bp
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1365 1365
Size: 5360
AccI GT'mk_AC
Cuts at: 2398 2398
Size: 5360
Acc65I G'GTAC_C
Cuts at: 2376 2376
Size: 5360
AclI AA'CG_TT
Cuts at: 5200 5200
Size: 5360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1688 3185 3617 3970 4632 1688
Size: 1497 432 353 662 2416
Fragments arranged by size:
2416 1497 662 432 353
AflIII A'CryG_T
Cuts at: 291 1659 2082 2712 4512 4793 291
Size: 1368 423 630 1800 281 858
Fragments arranged by size:
1800 1368 858 630 423 281
AhdI GACnn_n'nnGTC
Cuts at: 645 645
Size: 5360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 879 879
Size: 5360
AlwNI CAG_nnn'CTG
Cuts at: 312 2665 4103 312
Size: 2353 1438 1569
Fragments arranged by size:
2353 1569 1438
ApaI G_GGCC'C
Cuts at: 2306 2306
Size: 5360
ApaLI G'TGCA_C
Cuts at: 4198 4198
Size: 5360
ApoI r'AATT_y
Cuts at: 729 906 2178 2255 2364 4713 4969 729
Size: 177 1272 77 109 2349 256 1120
Fragments arranged by size:
2349 1272 1120 256 177 109 77
AseI AT'TA_AT
Cuts at: 20 20
Size: 5360
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
AvaI C'yCGr_G
Cuts at: 317 1577 1724 2380 317
Size: 1260 147 656 3297
Fragments arranged by size:
3297 1260 656 147
BamHI G'GATC_C
Cuts at: 2385 2385
Size: 5360
BanI G'GyrC_C
Cuts at: 1033 1522 2376 2790 3038 3073 1033
Size: 489 854 414 248 35 3320
Fragments arranged by size:
3320 854 489 414 248 35
BanII G_rGCy'C
Cuts at: 616 747 952 1295 2004 2251 2306 2374 3404
Size: 131 205 343 709 247 55 68 1030
Cuts at: 3404 616
Size: 2572
Fragments arranged by size:
2572 1030 709 343 247 205 131 68
55
BbeI G_GCGC'C
Cuts at: 2794 3042 2794
Size: 248 5112
BciVI GTATCCnnnnn_n'
Cuts at: 3258 4314 5360 3258
Size: 1056 1046 3258
Fragments arranged by size:
3258 1056 1046
BclI T'GATC_A
Cuts at: 1971 2880 1971
Size: 909 4451
BglI GCCn_nnn'nGGC
Cuts at: 143 1720 143
Size: 1577 3783
BglII A'GATC_T
Cuts at: 2875 4912 2875
Size: 2037 3323
BlpI GC'TnA_GC
Cuts at: 760 2472 760
Size: 1712 3648
Bme1580I G_kGCm'C
Cuts at: 2306 2985 3078 4202 2306
Size: 679 93 1124 3464
Fragments arranged by size:
3464 1124 679 93
BmgBI CAC'GTC
Cuts at: 328 328
Size: 5360
BmrI ACTGGGnnnn_n'
Cuts at: 2572 2653 2988 5132 2572
Size: 81 335 2144 2800
Fragments arranged by size:
2800 2144 335 81
BmtI G_CTAG'C
Cuts at: 352 352
Size: 5360
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 619 1697 3727 619
Size: 1078 2030 2252
Fragments arranged by size:
2252 2030 1078
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 719 869 1370 1388 1406 1424 1918 2030
Size: 521 150 501 18 18 18 494 112
Cuts at: 2030 198
Size: 3528
Fragments arranged by size:
3528 521 501 494 150 112 18 18
18
Bpu10I CC'TnA_GC
Cuts at: 1184 1184
Size: 5360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 1936 2536 3415 4129 4427 523
Size: 1413 600 879 714 298 1456
Fragments arranged by size:
1456 1413 879 714 600 298
BsaI GGTCTCn'nnnn_
Cuts at: 1473 5064 1473
Size: 3591 1769
BsaAI yAC'GTr
Cuts at: 292 2713 292
Size: 2421 2939
BsaBI GATnn'nnATC
Cuts at: 6 2874 4911 6
Size: 2868 2037 455
Fragments arranged by size:
2868 2037 455
BsaHI Gr'CG_yC
Cuts at: 799 2791 3039 3741 5237 799
Size: 1992 248 702 1496 922
Fragments arranged by size:
1992 1496 922 702 248
BsaWI w'CCGG_w
Cuts at: 2549 3070 3748 4159 4306 4580 2549
Size: 521 678 411 147 274 3329
Fragments arranged by size:
3329 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2034 2064 2034
Size: 30 5330
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 472 480 496 520 696 1552 1623 1705
Size: 18 8 16 24 176 856 71 82
Cuts at: 1705 1708 1750 1774 1798 1933 454
Size: 3 42 24 24 135 3881
Fragments arranged by size:
3881 856 176 135 82 71 42 24
24 24 18 16 8 3
BseYI C'CCAG_C
Cuts at: 1398 1416 1434 1458 2785 3543 4208 4616 1398
Size: 18 18 24 1327 758 665 408 2142
Fragments arranged by size:
2142 1327 758 665 408 24 18 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 878 2012 650
Size: 228 1134 3998
Fragments arranged by size:
3998 1134 228
BsiEI CG_ry'CG
Cuts at: 104 2902 2948 4178 104
Size: 2798 46 1230 1286
Fragments arranged by size:
2798 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 747 1314 2004 2125 2374 3152 3342 4202 747
Size: 567 690 121 249 778 190 860 1905
Fragments arranged by size:
1905 860 778 690 567 249 190 121
BsmI GAATG_Cn'
Cuts at: 1497 1497
Size: 5360
Bsp1286I G_dGCh'C
Cuts at: 616 747 952 1295 1314 2004 2125 2251 2306
Size: 131 205 343 19 690 121 126 55
Cuts at: 2306 2374 2985 3078 3152 3342 3404 4202 616
Size: 68 611 93 74 190 62 798 1774
Fragments arranged by size:
1774 798 690 611 343 205 190 131
126 121 93 74 68 62 55 19
BspHI T'CATG_A
Cuts at: 3792 3792
Size: 5360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1365 2410 2624 2926 3307 1365
Size: 1045 214 302 381 3418
Fragments arranged by size:
3418 1045 381 302 214
BsrBI CCG'CTC
Cuts at: 50 423 462 1350 1764 1779 3652 3706 5190
Size: 373 39 888 414 15 1873 54 1484
Cuts at: 5190 5355 50
Size: 165 55
Fragments arranged by size:
1873 1484 888 414 373 165 55 54
39 15
BsrDI GCAATG_nn'
Cuts at: 3272 3272
Size: 5360
BsrFI r'CCGG_y
Cuts at: 302 3358 3539 302
Size: 3056 181 2123
Fragments arranged by size:
3056 2123 181
BsrGI T'GTAC_A
Cuts at: 2038 2038
Size: 5360
BssHII G'CGCG_C
Cuts at: 1794 1794
Size: 5360
BssSI C'ACGA_G
Cuts at: 3631 4339 3631
Size: 708 4652
BstAPI GCAn_nnn'nTGC
Cuts at: 2737 2737
Size: 5360
BstBI TT'CG_AA
Cuts at: 670 3721 670
Size: 3051 2309
BstEII G'GTnAC_C
Cuts at: 2088 2088
Size: 5360
BstYI r'GATC_y
Cuts at: 1 633 1257 2209 2385 2852 2875 3210 3456
Size: 632 624 952 176 467 23 335 246
Cuts at: 3456 3762 3774 3860 4912 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 952 632 624 467 449 335 306
246 176 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 944 2312 944
Size: 1368 3992
BtgI C'CryG_G
Cuts at: 107 606 766 1391 1463 107
Size: 499 160 625 72 4004
Fragments arranged by size:
4004 625 499 160 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3310 3491 3654 3310
Size: 181 163 5016
Fragments arranged by size:
5016 181 163
BtsI GCAGTG_nn'
Cuts at: 164 2603 164
Size: 2439 2921
DraI TTT'AAA
Cuts at: 2359 2359
Size: 5360
DrdI GACnn_nn'nnGTC
Cuts at: 3066 4410 3066
Size: 1344 4016
EaeI y'GGCC_r
Cuts at: 135 818 2945 3119 3510 3537 4882 135
Size: 683 2127 174 391 27 1345 613
Fragments arranged by size:
2127 1345 683 613 391 174 27
EagI C'GGCC_G
Cuts at: 2945 2945
Size: 5360
EarI CTCTTCn'nnn_
Cuts at: 377 413 424 714 957 1024 1118 1340 1558
Size: 36 11 290 243 67 94 222 218
Cuts at: 1558 1616 1664 1877 3383 3593 377
Size: 58 48 213 1506 210 2144
Fragments arranged by size:
2144 1506 290 243 222 218 213 210
94 67 58 48 36 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 269 1204 1771 3568 4310 4456 5170 127
Size: 142 935 567 1797 742 146 714 317
Fragments arranged by size:
1797 935 742 714 567 317 146 142
EcoICRI GAG'CTC
Cuts at: 745 2002 2372 745
Size: 1257 370 3733
Fragments arranged by size:
3733 1257 370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 719 869 1370 1388 1406 1424 1688 1918
Size: 521 150 501 18 18 18 264 230
Cuts at: 1918 2030 3185 3617 3970 4632 198
Size: 112 1155 432 353 662 926
Fragments arranged by size:
1155 926 662 521 501 432 353 264
230 150 112 18 18 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2311 2311
Size: 5360
EcoO109I rG'GnC_Cy
Cuts at: 2303 2499 2303
Size: 196 5164
EcoRI G'AATT_C
Cuts at: 2364 2364
Size: 5360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 3379 2314
Size: 1065 4295
FspI TGC'GCA
Cuts at: 3141 3141
Size: 5360
HaeII r_GCGC'y
Cuts at: 1771 1896 2794 3042 4272 5043 1771
Size: 125 898 248 1230 771 2088
Fragments arranged by size:
2088 1230 898 771 248 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 631 663 911 943 1681 1713 2216 2248 3202
Size: 32 248 32 738 32 503 32 954
Cuts at: 3202 3232 3234 3264 631
Size: 30 2 30 2727
Fragments arranged by size:
2727 954 738 503 248 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 786 2399 786
Size: 1613 3747
HpaI GTT'AAC
Cuts at: 786 786
Size: 5360
KasI G'GCGC_C
Cuts at: 2790 3038 2790
Size: 248 5112
KpnI G_GTAC'C
Cuts at: 2380 2380
Size: 5360
MluI A'CGCG_T
Cuts at: 4793 4793
Size: 5360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 416 4119 4303 416
Size: 3703 184 1473
Fragments arranged by size:
3703 1473 184
MscI TGG'CCA
Cuts at: 820 3121 820
Size: 2301 3059
MslI CAynn'nnrTG
Cuts at: 877 2034 3349 3476 877
Size: 1157 1315 127 2761
Fragments arranged by size:
2761 1315 1157 127
MspA1I CmG'CkG
Cuts at: 309 838 1743 1749 2471 2785 3145 3929 4174
Size: 529 905 6 722 314 360 784 245
Cuts at: 4174 309
Size: 1495
Fragments arranged by size:
1495 905 784 722 529 360 314 245
6
NaeI GCC'GGC
Cuts at: 3541 3541
Size: 5360
NarI GG'CG_CC
Cuts at: 2791 3039 2791
Size: 248 5112
NcoI C'CATG_G
Cuts at: 107 606 766 1391 1463 107
Size: 499 160 625 72 4004
Fragments arranged by size:
4004 625 499 160 72
NgoMIV G'CCGG_C
Cuts at: 3539 3539
Size: 5360
NheI G'CTAG_C
Cuts at: 348 348
Size: 5360
NspI r_CATG'y
Cuts at: 2086 2413 2913 4516 2086
Size: 327 500 1603 2930
Fragments arranged by size:
2930 1603 500 327
PciI A'CATG_T
Cuts at: 2082 4512 2082
Size: 2430 2930
PflMI CCAn_nnn'nTGG
Cuts at: 1372 1372
Size: 5360
PfoI T'CCnGG_A
Cuts at: 2052 2052
Size: 5360
PmeI GTTT'AAAC
Cuts at: 2359 2359
Size: 5360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 3806
Size: 32 5328
PsiI TTA'TAA
Cuts at: 2194 2194
Size: 5360
PspOMI G'GGCC_C
Cuts at: 2302 2302
Size: 5360
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1935 1967 2309 2341 1935
Size: 32 342 32 4954
Fragments arranged by size:
4954 342 32 32
PstI C_TGCA'G
Cuts at: 758 1521 1748 2346 2407 3092 758
Size: 763 227 598 61 685 3026
Fragments arranged by size:
3026 763 685 598 227 61
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5360
PvuII CAG'CTG
Cuts at: 838 1743 2785 3145 838
Size: 905 1042 360 3053
Fragments arranged by size:
3053 1042 905 360
RsrII CG'GwC_CG
Cuts at: 3555 3555
Size: 5360
SacI G_AGCT'C
Cuts at: 747 2004 2374 747
Size: 1257 370 3733
Fragments arranged by size:
3733 1257 370
SalI G'TCGA_C
Cuts at: 2397 2397
Size: 5360
SapI GCTCTTCn'nnn_
Cuts at: 377 413 957 1340 1616 1877 3383 3593 377
Size: 36 544 383 276 261 1506 210 2144
Fragments arranged by size:
2144 1506 544 383 276 261 210 36
SbfI CC_TGCA'GG
Cuts at: 2407 2407
Size: 5360
ScaI AGT'ACT
Cuts at: 4877 4877
Size: 5360
SfcI C'TryA_G
Cuts at: 33 754 1517 1744 1952 2342 2403 3088 4056
Size: 721 763 227 208 390 61 685 968
Cuts at: 4056 4247 33
Size: 191 1146
Fragments arranged by size:
1146 968 763 721 685 390 227 208
191 61
SfoI GGC'GCC
Cuts at: 2792 3040 2792
Size: 248 5112
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
SmaI CCC'GGG
Cuts at: 319 1726 2382 319
Size: 1407 656 3297
Fragments arranged by size:
3297 1407 656
SmlI C'TyrA_G
Cuts at: 502 1915 2515 3430 4144 4406 502
Size: 1413 600 915 714 262 1456
Fragments arranged by size:
1456 1413 915 714 600 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5360
SphI G_CATG'C
Cuts at: 2413 2913 2413
Size: 500 4860
StyI C'CwwG_G
Cuts at: 107 606 766 1391 1463 1994 2494 2848 107
Size: 499 160 625 72 531 500 354 2619
Fragments arranged by size:
2619 625 531 500 499 354 160 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1149 1155 2943 3076 3535 3744 1149
Size: 6 1788 133 459 209 2765
Fragments arranged by size:
2765 1788 459 209 133 6
TatI w'GTAC_w
Cuts at: 1014 2038 4875 1014
Size: 1024 2837 1499
Fragments arranged by size:
2837 1499 1024
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 797 829 2006 2038 4877 4909 797
Size: 32 1177 32 2839 32 1248
Fragments arranged by size:
2839 1248 1177 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3157 3157
Size: 5360
XbaI T'CTAG_A
Cuts at: 901 2391 901
Size: 1490 3870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1461 2855 1461
Size: 1394 3966
XmaI C'CCGG_G
Cuts at: 317 1724 2380 317
Size: 1407 656 3297
Fragments arranged by size:
3297 1407 656
XmnI GAAnn'nnTTC
Cuts at: 1870 1870
Size: 5360
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PciI PflMI PfoI PmeI PpiI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BsiWI BsmBI BspEI
BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII MfeI NdeI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI