Restriction map for
pF1KB1292
(Circular) (Six-base) MAPSORT of: pF1KB1292.seq Check: 3697 from: 1 to: 5069
pF1KB1292 5069 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1276 1276
Size: 5069
AccI GT'mk_AC
Cuts at: 2107 2107
Size: 5069
Acc65I G'GTAC_C
Cuts at: 499 2085 499
Size: 1586 3483
AclI AA'CG_TT
Cuts at: 4909 4909
Size: 5069
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2894 3326 3679 4341 2894
Size: 432 353 662 3622
Fragments arranged by size:
3622 662 432 353
AfeI AGC'GCT
Cuts at: 2019 2019
Size: 5069
AflIII A'CryG_T
Cuts at: 2421 4221 4502 2421
Size: 1800 281 2988
Fragments arranged by size:
2988 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1368 1368
Size: 5069
AleI CACnn'nnGTG
Cuts at: 1852 1852
Size: 5069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 434 434
Size: 5069
AlwNI CAG_nnn'CTG
Cuts at: 2374 3812 2374
Size: 1438 3631
ApaI G_GGCC'C
Cuts at: 1207 1207
Size: 5069
ApaLI G'TGCA_C
Cuts at: 1147 1300 3907 1147
Size: 153 2607 2309
Fragments arranged by size:
2607 2309 153
ApoI r'AATT_y
Cuts at: 605 2073 4422 4678 605
Size: 1468 2349 256 996
Fragments arranged by size:
2349 1468 996 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5069
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5069
AvaI C'yCGr_G
Cuts at: 1028 1158 1207 1407 1549 2089 1028
Size: 130 49 200 142 540 4008
Fragments arranged by size:
4008 540 200 142 130 49
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 200 233 200
Size: 33 5036
BamHI G'GATC_C
Cuts at: 1928 2048 2094 1928
Size: 120 46 4903
Fragments arranged by size:
4903 120 46
BanI G'GyrC_C
Cuts at: 295 499 934 1093 1955 2085 2499 2747 2782
Size: 204 435 159 862 130 414 248 35
Cuts at: 2782 295
Size: 2582
Fragments arranged by size:
2582 862 435 414 248 204 159 130
35
BanII G_rGCy'C
Cuts at: 223 1166 1207 1909 2083 3113 223
Size: 943 41 702 174 1030 2179
Fragments arranged by size:
2179 1030 943 702 174 41
BbeI G_GCGC'C
Cuts at: 299 1959 2503 2751 299
Size: 1660 544 248 2617
Fragments arranged by size:
2617 1660 544 248
BbsI GAAGACnn'nnnn_
Cuts at: 632 632
Size: 5069
BbvCI CC'TCA_GC
Cuts at: 1839 1839
Size: 5069
BciVI GTATCCnnnnn_n'
Cuts at: 2967 4023 5069 2967
Size: 1056 1046 2967
Fragments arranged by size:
2967 1056 1046
BclI T'GATC_A
Cuts at: 2589 2589
Size: 5069
BfrBI ATG'CAT
Cuts at: 251 251
Size: 5069
BglII A'GATC_T
Cuts at: 1173 2584 4621 1173
Size: 1411 2037 1621
Fragments arranged by size:
2037 1621 1411
BlpI GC'TnA_GC
Cuts at: 2181 2181
Size: 5069
Bme1580I G_kGCm'C
Cuts at: 654 1151 1207 1304 2694 2787 3911 654
Size: 497 56 97 1390 93 1124 1812
Fragments arranged by size:
1812 1390 1124 497 97 93 56
BmgBI CAC'GTC
Cuts at: 336 1626 336
Size: 1290 3779
BmrI ACTGGGnnnn_n'
Cuts at: 652 907 1000 2281 2362 2697 4841 652
Size: 255 93 1281 81 335 2144 880
Fragments arranged by size:
2144 1281 880 335 255 93 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1854 3436 1854
Size: 1582 3487
Bpu10I CC'TnA_GC
Cuts at: 1839 1839
Size: 5069
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 2245 3124 3838 4136 258
Size: 1987 879 714 298 1191
Fragments arranged by size:
1987 1191 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4773 4773
Size: 5069
BsaAI yAC'GTr
Cuts at: 2422 2422
Size: 5069
BsaBI GATnn'nnATC
Cuts at: 6 2583 4620 6
Size: 2577 2037 455
Fragments arranged by size:
2577 2037 455
BsaHI Gr'CG_yC
Cuts at: 296 1477 1956 2500 2748 3450 4946 296
Size: 1181 479 544 248 702 1496 419
Fragments arranged by size:
1496 1181 702 544 479 419 248
BsaWI w'CCGG_w
Cuts at: 2258 2779 3457 3868 4015 4289 2258
Size: 521 678 411 147 274 3038
Fragments arranged by size:
3038 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 704 734 1113 1143 704
Size: 30 379 30 4630
Fragments arranged by size:
4630 379 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1225 1465 1537 1540 1549 1742 1225
Size: 240 72 3 9 193 4552
Fragments arranged by size:
4552 240 193 72 9 3
BseYI C'CCAG_C
Cuts at: 373 2005 2494 3252 3917 4325 373
Size: 1632 489 758 665 408 1117
Fragments arranged by size:
1632 1117 758 665 489 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 1165 1725 532
Size: 633 560 3876
Fragments arranged by size:
3876 633 560
BsiEI CG_ry'CG
Cuts at: 104 1829 2611 2657 3887 104
Size: 1725 782 46 1230 1286
Fragments arranged by size:
1725 1286 1230 782 46
BsiHKAI G_wGCw'C
Cuts at: 717 725 1050 1151 1304 2083 2861 3051 3911
Size: 8 325 101 153 779 778 190 860
Cuts at: 3911 717
Size: 1875
Fragments arranged by size:
1875 860 779 778 325 190 153 101
8
BsmBI CGTCTCn'nnnn_
Cuts at: 346 346
Size: 5069
Bsp1286I G_dGCh'C
Cuts at: 223 654 717 725 1050 1151 1166 1207 1304
Size: 431 63 8 325 101 15 41 97
Cuts at: 1304 1909 2083 2694 2787 2861 3051 3113 3911
Size: 605 174 611 93 74 190 62 798
Cuts at: 3911 223
Size: 1381
Fragments arranged by size:
1381 798 611 605 431 325 190 174
101 97 93 74 63 62 41 15
8
BspHI T'CATG_A
Cuts at: 3501 3501
Size: 5069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1276 1569 2119 2333 2635 3016 1276
Size: 293 550 214 302 381 3329
Fragments arranged by size:
3329 550 381 302 293 214
BsrBI CCG'CTC
Cuts at: 50 966 1934 3361 3415 4899 5064 50
Size: 916 968 1427 54 1484 165 55
Fragments arranged by size:
1484 1427 968 916 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1626 2981 1626
Size: 1355 3714
BsrFI r'CCGG_y
Cuts at: 277 1238 3067 3248 277
Size: 961 1829 181 2098
Fragments arranged by size:
2098 1829 961 181
BsrGI T'GTAC_A
Cuts at: 344 704 344
Size: 360 4709
BssSI C'ACGA_G
Cuts at: 866 3340 4048 866
Size: 2474 708 1887
Fragments arranged by size:
2474 1887 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2446 2446
Size: 5069
BstBI TT'CG_AA
Cuts at: 3430 3430
Size: 5069
BstEII G'GTnAC_C
Cuts at: 231 231
Size: 5069
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5069
BstYI r'GATC_y
Cuts at: 1 1173 1928 2048 2094 2561 2584 2919 3165
Size: 1172 755 120 46 467 23 335 246
Cuts at: 3165 3471 3483 3569 4621 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1172 1052 755 467 449 335 306 246
120 86 46 23 12
Bsu36I CC'TnA_GG
Cuts at: 430 430
Size: 5069
BtgI C'CryG_G
Cuts at: 107 113 215 389 814 1062 1781 1919 107
Size: 6 102 174 425 248 719 138 3257
Fragments arranged by size:
3257 719 425 248 174 138 102 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3019 3200 3363 3019
Size: 181 163 4725
Fragments arranged by size:
4725 181 163
BtsI GCAGTG_nn'
Cuts at: 453 520 2312 453
Size: 67 1792 3210
Fragments arranged by size:
3210 1792 67
DraI TTT'AAA
Cuts at: 921 2068 921
Size: 1147 3922
DraIII CAC_nnn'GTG
Cuts at: 1146 1146
Size: 5069
DrdI GACnn_nn'nnGTC
Cuts at: 2775 4119 2775
Size: 1344 3725
EaeI y'GGCC_r
Cuts at: 110 1110 1487 1916 2654 2828 3219 3246 4591
Size: 1000 377 429 738 174 391 27 1345
Cuts at: 4591 110
Size: 588
Fragments arranged by size:
1345 1000 738 588 429 391 377 174
27
EagI C'GGCC_G
Cuts at: 2654 2654
Size: 5069
EarI CTCTTCn'nnn_
Cuts at: 808 1038 1378 1761 2039 3092 3302 808
Size: 230 340 383 278 1053 210 2575
Fragments arranged by size:
2575 1053 383 340 278 230 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1450 1462 3277 4019 4165 4879 1450
Size: 12 1815 742 146 714 1640
Fragments arranged by size:
1815 1640 742 714 146 12
EcoICRI GAG'CTC
Cuts at: 2081 2081
Size: 5069
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2894 3326 3679 4341 2894
Size: 432 353 662 3622
Fragments arranged by size:
3622 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 429 889 429
Size: 460 4609
EcoO109I rG'GnC_Cy
Cuts at: 995 1203 1204 1512 1581 1601 1791 2208 995
Size: 208 1 308 69 20 190 417 3856
Fragments arranged by size:
3856 417 308 208 190 69 20 1
EcoRI G'AATT_C
Cuts at: 2073 2073
Size: 5069
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 5069
FspI TGC'GCA
Cuts at: 2850 2850
Size: 5069
HaeII r_GCGC'y
Cuts at: 299 1959 2021 2503 2751 3981 4752 299
Size: 1660 62 482 248 1230 771 616
Fragments arranged by size:
1660 1230 771 616 482 248 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1113 1145 1354 1386 1390 1422 1721 1753 2911
Size: 32 209 32 4 32 299 32 1158
Cuts at: 2911 2941 2943 2973 1113
Size: 30 2 30 3209
Fragments arranged by size:
3209 1158 299 209 32 32 32 32
30 30 4 2
HincII GTy'rAC
Cuts at: 2108 2108
Size: 5069
KasI G'GCGC_C
Cuts at: 295 1955 2499 2747 295
Size: 1660 544 248 2617
Fragments arranged by size:
2617 1660 544 248
KpnI G_GTAC'C
Cuts at: 503 2089 503
Size: 1586 3483
MluI A'CGCG_T
Cuts at: 4502 4502
Size: 5069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1112 1460 1713 1889 3828 4012 1112
Size: 348 253 176 1939 184 2169
Fragments arranged by size:
2169 1939 348 253 184 176
MscI TGG'CCA
Cuts at: 112 2830 112
Size: 2718 2351
MslI CAynn'nnrTG
Cuts at: 112 334 1852 1975 3058 3185 112
Size: 222 1518 123 1083 127 1996
Fragments arranged by size:
1996 1518 1083 222 127 123
MspA1I CmG'CkG
Cuts at: 403 414 1817 2180 2494 2854 3638 3883 403
Size: 11 1403 363 314 360 784 245 1589
Fragments arranged by size:
1589 1403 784 363 360 314 245 11
NaeI GCC'GGC
Cuts at: 3250 3250
Size: 5069
NarI GG'CG_CC
Cuts at: 296 1956 2500 2748 296
Size: 1660 544 248 2617
Fragments arranged by size:
2617 1660 544 248
NcoI C'CATG_G
Cuts at: 107 113 389 1062 1781 107
Size: 6 276 673 719 3395
Fragments arranged by size:
3395 719 673 276 6
NdeI CA'TA_TG
Cuts at: 2058 2058
Size: 5069
NgoMIV G'CCGG_C
Cuts at: 3248 3248
Size: 5069
NsiI A_TGCA'T
Cuts at: 253 253
Size: 5069
NspI r_CATG'y
Cuts at: 251 2122 2622 4225 251
Size: 1871 500 1603 1095
Fragments arranged by size:
1871 1603 1095 500
PciI A'CATG_T
Cuts at: 4221 4221
Size: 5069
PflMI CCAn_nnn'nTGG
Cuts at: 1782 1782
Size: 5069
PmeI GTTT'AAAC
Cuts at: 2068 2068
Size: 5069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 3515
Size: 32 5037
PpuMI rG'GwC_Cy
Cuts at: 995 1512 1581 1601 1791 995
Size: 517 69 20 190 4273
Fragments arranged by size:
4273 517 190 69 20
PspOMI G'GGCC_C
Cuts at: 1203 1203
Size: 5069
PspXI vC'TCGA_Gb
Cuts at: 1407 1407
Size: 5069
PstI C_TGCA'G
Cuts at: 1521 1580 2116 2801 1521
Size: 59 536 685 3789
Fragments arranged by size:
3789 685 536 59
PvuI CG_AT'CG
Cuts at: 104 1829 104
Size: 1725 3344
PvuII CAG'CTG
Cuts at: 414 2494 2854 414
Size: 2080 360 2629
Fragments arranged by size:
2629 2080 360
RsrII CG'GwC_CG
Cuts at: 3264 3264
Size: 5069
SacI G_AGCT'C
Cuts at: 2083 2083
Size: 5069
SalI G'TCGA_C
Cuts at: 2106 2106
Size: 5069
SapI GCTCTTCn'nnn_
Cuts at: 808 1038 2039 3092 3302 808
Size: 230 1001 1053 210 2575
Fragments arranged by size:
2575 1053 1001 230 210
SbfI CC_TGCA'GG
Cuts at: 1580 2116 1580
Size: 536 4533
ScaI AGT'ACT
Cuts at: 4586 4586
Size: 5069
SexAI A'CCwGG_T
Cuts at: 930 930
Size: 5069
SfcI C'TryA_G
Cuts at: 33 473 1517 1576 2112 2797 3765 3956 33
Size: 440 1044 59 536 685 968 191 1146
Fragments arranged by size:
1146 1044 968 685 536 440 191 59
SfoI GGC'GCC
Cuts at: 297 1957 2501 2749 297
Size: 1660 544 248 2617
Fragments arranged by size:
2617 1660 544 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5069
SmaI CCC'GGG
Cuts at: 2091 2091
Size: 5069
SmlI C'TyrA_G
Cuts at: 237 1407 2224 3139 3853 4115 237
Size: 1170 817 915 714 262 1191
Fragments arranged by size:
1191 1170 915 817 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5069
SphI G_CATG'C
Cuts at: 251 2122 2622 251
Size: 1871 500 2698
Fragments arranged by size:
2698 1871 500
SspI AAT'ATT
Cuts at: 266 266
Size: 5069
StuI AGG'CCT
Cuts at: 1748 1748
Size: 5069
StyI C'CwwG_G
Cuts at: 107 113 389 938 1062 1781 2203 2557 107
Size: 6 276 549 124 719 422 354 2619
Fragments arranged by size:
2619 719 549 422 354 276 124 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1630 2652 2785 3244 3453 1630
Size: 1022 133 459 209 3246
Fragments arranged by size:
3246 1022 459 209 133
TatI w'GTAC_w
Cuts at: 344 594 704 4584 344
Size: 250 110 3880 829
Fragments arranged by size:
3880 829 250 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 704 736 4586 4618 704
Size: 32 3850 32 1155
Fragments arranged by size:
3850 1155 32 32
Tth111I GACn'n_nGTC
Cuts at: 2866 2866
Size: 5069
XbaI T'CTAG_A
Cuts at: 2100 2100
Size: 5069
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1644 2564 1644
Size: 920 4149
XhoI C'TCGA_G
Cuts at: 1407 1407
Size: 5069
XmaI C'CCGG_G
Cuts at: 2089 2089
Size: 5069
XmnI GAAnn'nnTTC
Cuts at: 314 1972 314
Size: 1658 3411
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AloI AscI AvrII BcgI BglI
BmtI BpmI BsiWI BsmI BspEI BssHII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI PacI PasI PfoI PmlI PshAI PsiI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI
ZraI