Restriction map for
pF1KB1326
(Circular) (Six-base) MAPSORT of: pF1KB1326.seq Check: 6265 from: 1 to: 5939
pF1KB1326 5939 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1539 2496 2760 1539
Size: 957 264 4718
Fragments arranged by size:
4718 957 264
AatII G_ACGT'C
Cuts at: 380 380
Size: 5939
AccI GT'mk_AC
Cuts at: 2977 2977
Size: 5939
Acc65I G'GTAC_C
Cuts at: 2090 2955 2090
Size: 865 5074
AclI AA'CG_TT
Cuts at: 5779 5779
Size: 5939
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 858 1027 1324 1804 3764 4196 4549 5211
Size: 428 169 297 480 1960 432 353 662
Cuts at: 5211 430
Size: 1158
Fragments arranged by size:
1960 1158 662 480 432 428 353 297
169
AfeI AGC'GCT
Cuts at: 948 948
Size: 5939
AflIII A'CryG_T
Cuts at: 1343 3291 5091 5372 1343
Size: 1948 1800 281 1910
Fragments arranged by size:
1948 1910 1800 281
AleI CACnn'nnGTG
Cuts at: 436 1693 2764 436
Size: 1257 1071 3611
Fragments arranged by size:
3611 1257 1071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 205 1678 205
Size: 1473 4466
AlwNI CAG_nnn'CTG
Cuts at: 608 1506 2824 3244 4682 608
Size: 898 1318 420 1438 1865
Fragments arranged by size:
1865 1438 1318 898 420
ApaI G_GGCC'C
Cuts at: 135 271 1113 1293 135
Size: 136 842 180 4781
Fragments arranged by size:
4781 842 180 136
ApaLI G'TGCA_C
Cuts at: 429 2836 4777 429
Size: 2407 1941 1591
Fragments arranged by size:
2407 1941 1591
ApoI r'AATT_y
Cuts at: 2943 5292 5548 2943
Size: 2349 256 3334
Fragments arranged by size:
3334 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5939
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5939
AvaI C'yCGr_G
Cuts at: 127 216 241 264 485 684 802 1123 1237
Size: 89 25 23 221 199 118 321 114
Cuts at: 1237 1429 1642 1990 2577 2799 2894 2959 127
Size: 192 213 348 587 222 95 65 3107
Fragments arranged by size:
3107 587 348 321 222 221 213 199
192 118 114 95 89 65 25 23
AvrII C'CTAG_G
Cuts at: 2929 2929
Size: 5939
BamHI G'GATC_C
Cuts at: 422 2964 422
Size: 2542 3397
BanI G'GyrC_C
Cuts at: 441 537 632 934 1078 2013 2071 2090 2955
Size: 96 95 302 144 935 58 19 865
Cuts at: 2955 3369 3617 3652 441
Size: 414 248 35 2728
Fragments arranged by size:
2728 935 865 414 302 248 144 96
95 58 35 19
BanII G_rGCy'C
Cuts at: 128 135 199 264 271 494 1113 1281 1293
Size: 7 64 65 7 223 619 168 12
Cuts at: 1293 1997 2315 2578 2599 2786 2953 3983 128
Size: 704 318 263 21 187 167 1030 2084
Fragments arranged by size:
2084 1030 704 619 318 263 223 187
168 167 65 64 21 12 7 7
BbeI G_GCGC'C
Cuts at: 938 2075 3373 3621 938
Size: 1137 1298 248 3256
Fragments arranged by size:
3256 1298 1137 248
BbsI GAAGACnn'nnnn_
Cuts at: 718 1953 718
Size: 1235 4704
BbvCI CC'TCA_GC
Cuts at: 2845 2845
Size: 5939
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2084 2118 2337 2371 2084
Size: 34 219 34 5652
Fragments arranged by size:
5652 219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3837 4893 5939 3837
Size: 1056 1046 3837
Fragments arranged by size:
3837 1056 1046
BclI T'GATC_A
Cuts at: 2810 3459 2810
Size: 649 5290
BglI GCCn_nnn'nGGC
Cuts at: 112 1910 2275 112
Size: 1798 365 3776
Fragments arranged by size:
3776 1798 365
BglII A'GATC_T
Cuts at: 1010 1307 3454 5491 1010
Size: 297 2147 2037 1458
Fragments arranged by size:
2147 2037 1458 297
BlpI GC'TnA_GC
Cuts at: 2368 3051 2368
Size: 683 5256
Bme1580I G_kGCm'C
Cuts at: 135 271 433 593 635 1113 1293 2018 2140
Size: 136 162 160 42 478 180 725 122
Cuts at: 2140 2807 2840 3564 3657 4781 135
Size: 667 33 724 93 1124 1293
Fragments arranged by size:
1293 1124 725 724 667 478 180 162
160 136 122 93 42 33
BmgBI CAC'GTC
Cuts at: 716 732 1473 1602 716
Size: 16 741 129 5053
Fragments arranged by size:
5053 741 129 16
BmrI ACTGGGnnnn_n'
Cuts at: 2528 3151 3232 3567 5711 2528
Size: 623 81 335 2144 2756
Fragments arranged by size:
2756 2144 623 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1939 2764 4306 1939
Size: 825 1542 3572
Fragments arranged by size:
3572 1542 825
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1114 1162 1411 2098 2668 553
Size: 561 48 249 687 570 3824
Fragments arranged by size:
3824 687 570 561 249 48
Bpu10I CC'TnA_GC
Cuts at: 198 2845 198
Size: 2647 3292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1309 1891 2737 3115 3994 4708 5006 1309
Size: 582 846 378 879 714 298 2242
Fragments arranged by size:
2242 879 846 714 582 378 298
BsaI GGTCTCn'nnnn_
Cuts at: 1910 5643 1910
Size: 3733 2206
BsaAI yAC'GTr
Cuts at: 417 1344 1572 2445 3292 417
Size: 927 228 873 847 3064
Fragments arranged by size:
3064 927 873 847 228
BsaBI GATnn'nnATC
Cuts at: 6 3453 5490 6
Size: 3447 2037 455
Fragments arranged by size:
3447 2037 455
BsaHI Gr'CG_yC
Cuts at: 254 377 819 935 1181 2072 2231 2669 3370
Size: 123 442 116 246 891 159 438 701
Cuts at: 3370 3618 4320 5816 254
Size: 248 702 1496 377
Fragments arranged by size:
1496 891 702 701 442 438 377 248
246 159 123 116
BsaWI w'CCGG_w
Cuts at: 296 984 1934 2707 2714 3128 3649 4327 4738
Size: 688 950 773 7 414 521 678 411
Cuts at: 4738 4885 5159 296
Size: 147 274 1076
Fragments arranged by size:
1076 950 773 688 678 521 414 411
274 147 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 322 352 530 560 1699 1729 1990 2020 2074
Size: 30 178 30 1139 30 261 30 54
Cuts at: 2074 2104 2185 2215 322
Size: 30 81 30 4046
Fragments arranged by size:
4046 1139 261 178 81 54 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 714 1099 1237 1660 1707 2095 2413 2740 2883
Size: 385 138 423 47 388 318 327 143
Cuts at: 2883 714
Size: 3770
Fragments arranged by size:
3770 423 388 385 327 318 143 138
47
BseYI C'CCAG_C
Cuts at: 144 192 445 1189 1201 2060 2168 2566 2570
Size: 48 253 744 12 859 108 398 4
Cuts at: 2570 3364 4122 4787 5195 144
Size: 794 758 665 408 888
Fragments arranged by size:
888 859 794 758 744 665 408 398
253 108 48 12 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1825 2306 2347 2410 2470 2788 553
Size: 1272 481 41 63 60 318 3704
Fragments arranged by size:
3704 1272 481 318 63 60 41
BsiEI CG_ry'CG
Cuts at: 104 122 1413 3481 3527 4757 104
Size: 18 1291 2068 46 1230 1286
Fragments arranged by size:
2068 1291 1286 1230 46 18
BsiHKAI G_wGCw'C
Cuts at: 433 1372 1829 2240 2840 2953 3731 3921 4781
Size: 939 457 411 600 113 778 190 860
Cuts at: 4781 433
Size: 1591
Fragments arranged by size:
1591 939 860 778 600 457 411 190
113
BsmBI CGTCTCn'nnnn_
Cuts at: 1608 1608
Size: 5939
Bsp1286I G_dGCh'C
Cuts at: 128 135 199 264 271 433 494 593 635
Size: 7 64 65 7 162 61 99 42
Cuts at: 635 1113 1281 1293 1372 1829 1997 2018 2140
Size: 478 168 12 79 457 168 21 122
Cuts at: 2140 2240 2315 2578 2599 2786 2807 2840 2953
Size: 100 75 263 21 187 21 33 113
Cuts at: 2953 3564 3657 3731 3921 3983 4781 128
Size: 611 93 74 190 62 798 1286
Fragments arranged by size:
1286 798 611 478 457 263 190 187
168 168 162 122 113 100 99 93
79 75 74 65 64 62 61 42
33 21 21 21 12 7 7
BspEI T'CCGG_A
Cuts at: 296 984 1934 2707 2714 296
Size: 688 950 773 7 3521
Fragments arranged by size:
3521 950 773 688 7
BspHI T'CATG_A
Cuts at: 396 4371 396
Size: 3975 1964
BspMI ACCTGCnnnn'nnnn_
Cuts at: 686 1539 1844 1928 2496 2760 2989 3203 3505
Size: 853 305 84 568 264 229 214 302
Cuts at: 3505 3886 686
Size: 381 2739
Fragments arranged by size:
2739 853 568 381 305 302 264 229
214 84
BsrBI CCG'CTC
Cuts at: 50 246 1379 1767 2514 4231 4285 5769 5934
Size: 196 1133 388 747 1717 54 1484 165
Cuts at: 5934 50
Size: 55
Fragments arranged by size:
1717 1484 1133 747 388 196 165 55
54
BsrDI GCAATG_nn'
Cuts at: 3851 3851
Size: 5939
BsrFI r'CCGG_y
Cuts at: 361 540 739 1075 1151 1466 1514 1805 3937
Size: 179 199 336 76 315 48 291 2132
Cuts at: 3937 4118 361
Size: 181 2182
Fragments arranged by size:
2182 2132 336 315 291 199 181 179
76 48
BsrGI T'GTAC_A
Cuts at: 1772 2675 1772
Size: 903 5036
BssHII G'CGCG_C
Cuts at: 209 211 691 209
Size: 2 480 5457
Fragments arranged by size:
5457 480 2
BssSI C'ACGA_G
Cuts at: 432 2272 4210 4918 432
Size: 1840 1938 708 1453
Fragments arranged by size:
1938 1840 1453 708
BstAPI GCAn_nnn'nTGC
Cuts at: 608 3316 608
Size: 2708 3231
BstBI TT'CG_AA
Cuts at: 4300 4300
Size: 5939
BstXI CCAn_nnnn'nTGG
Cuts at: 628 1265 2262 2278 628
Size: 637 997 16 4289
Fragments arranged by size:
4289 997 637 16
BstYI r'GATC_y
Cuts at: 1 422 1010 1307 2495 2964 3431 3454 3789
Size: 421 588 297 1188 469 467 23 335
Cuts at: 3789 4035 4341 4353 4439 5491 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1188 1052 588 469 467 449 421 335
306 297 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2831 2831
Size: 5939
BtgI C'CryG_G
Cuts at: 107 788 908 953 1285 1691 107
Size: 681 120 45 332 406 4355
Fragments arranged by size:
4355 681 406 332 120 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1471 2060 3889 4070 4233 1471
Size: 589 1829 181 163 3177
Fragments arranged by size:
3177 1829 589 181 163
BtsI GCAGTG_nn'
Cuts at: 471 549 609 1102 1814 2289 3182 471
Size: 78 60 493 712 475 893 3228
Fragments arranged by size:
3228 893 712 493 475 78 60
DraI TTT'AAA
Cuts at: 2938 2938
Size: 5939
DrdI GACnn_nn'nnGTC
Cuts at: 3645 4989 3645
Size: 1344 4595
EaeI y'GGCC_r
Cuts at: 119 359 737 1073 1134 1410 2120 3524 3698
Size: 240 378 336 61 276 710 1404 174
Cuts at: 3698 4089 4116 5461 119
Size: 391 27 1345 597
Fragments arranged by size:
1404 1345 710 597 391 378 336 276
240 174 61 27
EagI C'GGCC_G
Cuts at: 119 1410 3524 119
Size: 1291 2114 2534
Fragments arranged by size:
2534 2114 1291
EarI CTCTTCn'nnn_
Cuts at: 1360 1991 2390 3962 4172 1360
Size: 631 399 1572 210 3127
Fragments arranged by size:
3127 1572 631 399 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1039 2914 4147 4889 5035 5749 1039
Size: 1875 1233 742 146 714 1229
Fragments arranged by size:
1875 1233 1229 742 714 146
EcoICRI GAG'CTC
Cuts at: 2951 2951
Size: 5939
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 553 858 1027 1114 1162 1324 1411 1804
Size: 123 305 169 87 48 162 87 393
Cuts at: 1804 2098 2668 3764 4196 4549 5211 430
Size: 294 570 1096 432 353 662 1158
Fragments arranged by size:
1158 1096 662 570 432 393 353 305
294 169 162 123 87 87 48
EcoO109I rG'GnC_Cy
Cuts at: 131 238 268 528 1128 1299 1395 1981 2193
Size: 107 30 260 600 171 96 586 212
Cuts at: 2193 2796 3078 131
Size: 603 282 2992
Fragments arranged by size:
2992 603 600 586 282 260 212 171
107 96 30
EcoRI G'AATT_C
Cuts at: 2943 2943
Size: 5939
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 339 3958 339
Size: 3619 2320
FspI TGC'GCA
Cuts at: 3720 3720
Size: 5939
HaeII r_GCGC'y
Cuts at: 547 796 938 950 2075 3373 3621 4851 5622
Size: 249 142 12 1125 1298 248 1230 771
Cuts at: 5622 547
Size: 864
Fragments arranged by size:
1298 1230 1125 864 771 249 248 142
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2185 2217 3781 3811 3813 3843 2185
Size: 32 1564 30 2 30 4281
Fragments arranged by size:
4281 1564 32 30 30 2
HincII GTy'rAC
Cuts at: 2978 2978
Size: 5939
KasI G'GCGC_C
Cuts at: 934 2071 3369 3617 934
Size: 1137 1298 248 3256
Fragments arranged by size:
3256 1298 1137 248
KpnI G_GTAC'C
Cuts at: 2094 2959 2094
Size: 865 5074
MluI A'CGCG_T
Cuts at: 5372 5372
Size: 5939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 365 1718 2624 4698 4882 365
Size: 1353 906 2074 184 1422
Fragments arranged by size:
2074 1422 1353 906 184
MscI TGG'CCA
Cuts at: 1136 2122 3700 1136
Size: 986 1578 3375
Fragments arranged by size:
3375 1578 986
MslI CAynn'nnrTG
Cuts at: 436 626 1693 1795 2764 3928 4055 436
Size: 190 1067 102 969 1164 127 2320
Fragments arranged by size:
2320 1164 1067 969 190 127 102
MspA1I CmG'CkG
Cuts at: 274 790 910 1287 1404 1443 1479 1865 2168
Size: 516 120 377 117 39 36 386 303
Cuts at: 2168 2186 2249 2570 2821 3050 3364 3724 4508
Size: 18 63 321 251 229 314 360 784
Cuts at: 4508 4753 274
Size: 245 1460
Fragments arranged by size:
1460 784 516 386 377 360 321 314
303 251 245 229 120 117 63 39
36 18
NaeI GCC'GGC
Cuts at: 363 741 1153 1468 1807 4120 363
Size: 378 412 315 339 2313 2182
Fragments arranged by size:
2313 2182 412 378 339 315
NarI GG'CG_CC
Cuts at: 935 2072 3370 3618 935
Size: 1137 1298 248 3256
Fragments arranged by size:
3256 1298 1137 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5939
NgoMIV G'CCGG_C
Cuts at: 361 739 1151 1466 1805 4118 361
Size: 378 412 315 339 2313 2182
Fragments arranged by size:
2313 2182 412 378 339 315
NspI r_CATG'y
Cuts at: 1779 2844 2992 3492 5095 1779
Size: 1065 148 500 1603 2623
Fragments arranged by size:
2623 1603 1065 500 148
PasI CC'CwG_GG
Cuts at: 2133 2724 2786 2133
Size: 591 62 5286
Fragments arranged by size:
5286 591 62
PciI A'CATG_T
Cuts at: 5091 5091
Size: 5939
PflMI CCAn_nnn'nTGG
Cuts at: 1446 1446
Size: 5939
PfoI T'CCnGG_A
Cuts at: 530 991 530
Size: 461 5478
PmeI GTTT'AAAC
Cuts at: 2938 2938
Size: 5939
PmlI CAC'GTG
Cuts at: 417 417
Size: 5939
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 667 699 2182 2214 4385 4417 667
Size: 32 1483 32 2171 32 2189
Fragments arranged by size:
2189 2171 1483 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 238 528 1128 1299 1395 1981 2193 238
Size: 290 600 171 96 586 212 3984
Fragments arranged by size:
3984 600 586 290 212 171 96
PshAI GACnn'nnGTC
Cuts at: 1865 2153 2668 1865
Size: 288 515 5136
Fragments arranged by size:
5136 515 288
PspOMI G'GGCC_C
Cuts at: 131 267 1109 1289 131
Size: 136 842 180 4781
Fragments arranged by size:
4781 842 180 136
PstI C_TGCA'G
Cuts at: 184 604 709 1930 2462 2507 2986 3671 184
Size: 420 105 1221 532 45 479 685 2452
Fragments arranged by size:
2452 1221 685 532 479 420 105 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5939
PvuII CAG'CTG
Cuts at: 1443 1865 2570 2821 3364 3724 1443
Size: 422 705 251 543 360 3658
Fragments arranged by size:
3658 705 543 422 360 251
RsrII CG'GwC_CG
Cuts at: 4134 4134
Size: 5939
SacI G_AGCT'C
Cuts at: 2953 2953
Size: 5939
SacII CC_GC'GG
Cuts at: 791 911 1288 791
Size: 120 377 5442
Fragments arranged by size:
5442 377 120
SalI G'TCGA_C
Cuts at: 2976 2976
Size: 5939
SanDI GG'GwC_CC
Cuts at: 238 238
Size: 5939
SapI GCTCTTCn'nnn_
Cuts at: 1360 3962 4172 1360
Size: 2602 210 3127
Fragments arranged by size:
3127 2602 210
SbfI CC_TGCA'GG
Cuts at: 2986 2986
Size: 5939
ScaI AGT'ACT
Cuts at: 5456 5456
Size: 5939
SexAI A'CCwGG_T
Cuts at: 2611 2611
Size: 5939
SfcI C'TryA_G
Cuts at: 33 180 464 600 705 1519 1926 2181 2458
Size: 147 284 136 105 814 407 255 277
Cuts at: 2458 2503 2982 3667 4635 4826 33
Size: 45 479 685 968 191 1146
Fragments arranged by size:
1146 968 814 685 479 407 284 277
255 191 147 136 105 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 1910 1910
Size: 5939
SfoI GGC'GCC
Cuts at: 936 2073 3371 3619 936
Size: 1137 1298 248 3256
Fragments arranged by size:
3256 1298 1137 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5939
SgrAI Cr'CCGG_yG
Cuts at: 540 540
Size: 5939
SmaI CCC'GGG
Cuts at: 129 218 266 487 1239 1992 2801 2896 2961
Size: 89 48 221 752 753 809 95 65
Cuts at: 2961 129
Size: 3107
Fragments arranged by size:
3107 809 753 752 221 95 89 65
48
SmlI C'TyrA_G
Cuts at: 1324 1906 2752 3094 4009 4723 4985 1324
Size: 582 846 342 915 714 262 2278
Fragments arranged by size:
2278 915 846 714 582 342 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5939
SphI G_CATG'C
Cuts at: 2992 3492 2992
Size: 500 5439
SrfI GCCC'GGGC
Cuts at: 2896 2896
Size: 5939
StuI AGG'CCT
Cuts at: 1174 1264 1904 2546 1174
Size: 90 640 642 4567
Fragments arranged by size:
4567 642 640 90
StyI C'CwwG_G
Cuts at: 107 1722 2151 2929 3073 3427 107
Size: 1615 429 778 144 354 2619
Fragments arranged by size:
2619 1615 778 429 354 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 459 593 664 2052 3522 3655 4114 4323 459
Size: 134 71 1388 1470 133 459 209 2075
Fragments arranged by size:
2075 1470 1388 459 209 134 133 71
TatI w'GTAC_w
Cuts at: 1649 1772 2675 5454 1649
Size: 123 903 2779 2134
Fragments arranged by size:
2779 2134 903 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 322 354 530 562 1699 1731 1990 2022 5456
Size: 32 176 32 1137 32 259 32 3434
Cuts at: 5456 5488 322
Size: 32 773
Fragments arranged by size:
3434 1137 773 259 176 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3736 3736
Size: 5939
XbaI T'CTAG_A
Cuts at: 2970 2970
Size: 5939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1689 2130 2692 3434 1689
Size: 441 562 742 4194
Fragments arranged by size:
4194 742 562 441
XmaI C'CCGG_G
Cuts at: 127 216 264 485 1237 1990 2799 2894 2959
Size: 89 48 221 752 753 809 95 65
Cuts at: 2959 127
Size: 3107
Fragments arranged by size:
3107 809 753 752 221 95 89 65
48
ZraI GAC'GTC
Cuts at: 378 378
Size: 5939
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI AscI BaeI BfrBI BmtI
BsiWI BsmI BstEII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PsiI PspXI PsrI SnaBI
SspI SwaI XhoI XmnI