(Circular) (Six-base) MAPSORT of: pF1KB1338.seq Check: 1541 from: 1 to: 4928 pF1KB1338 4928 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 483 483 Size: 4928 AatII G_ACGT'C Cuts at: 727 727 Size: 4928 AccI GT'mk_AC Cuts at: 1966 1966 Size: 4928 Acc65I G'GTAC_C Cuts at: 1944 1944 Size: 4928 AclI AA'CG_TT Cuts at: 4768 4768 Size: 4928 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 594 2753 3185 3538 4200 594 Size: 2159 432 353 662 1322 Fragments arranged by size: 2159 1322 662 432 353 AfeI AGC'GCT Cuts at: 1620 1620 Size: 4928 AflIII A'CryG_T Cuts at: 326 2280 4080 4361 326 Size: 1954 1800 281 893 Fragments arranged by size: 1954 1800 893 281 AhdI GACnn_n'nnGTC Cuts at: 1062 1062 Size: 4928 AlwNI CAG_nnn'CTG Cuts at: 1350 1854 2233 3671 1350 Size: 504 379 1438 2607 Fragments arranged by size: 2607 1438 504 379 ApaI G_GGCC'C Cuts at: 211 1175 1197 211 Size: 964 22 3942 Fragments arranged by size: 3942 964 22 ApaLI G'TGCA_C Cuts at: 148 3766 148 Size: 3618 1310 ApoI r'AATT_y Cuts at: 1932 4281 4537 1932 Size: 2349 256 2323 Fragments arranged by size: 2349 2323 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4928 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4928 AvaI C'yCGr_G Cuts at: 644 1496 1513 1948 644 Size: 852 17 435 3624 Fragments arranged by size: 3624 852 435 17 BamHI G'GATC_C Cuts at: 1033 1953 1033 Size: 920 4008 BanI G'GyrC_C Cuts at: 128 1279 1572 1728 1906 1944 2358 2606 2641 Size: 1151 293 156 178 38 414 248 35 Cuts at: 2641 128 Size: 2415 Fragments arranged by size: 2415 1151 414 293 248 178 156 38 35 BanII G_rGCy'C Cuts at: 211 373 652 1142 1175 1197 1741 1873 1942 Size: 162 279 490 33 22 544 132 69 Cuts at: 1942 2972 211 Size: 1030 2167 Fragments arranged by size: 2167 1030 544 490 279 162 132 69 33 22 BbeI G_GCGC'C Cuts at: 132 1732 2362 2610 132 Size: 1600 630 248 2450 Fragments arranged by size: 2450 1600 630 248 BbsI GAAGACnn'nnnn_ Cuts at: 1812 1812 Size: 4928 BbvCI CC'TCA_GC Cuts at: 1533 1533 Size: 4928 BciVI GTATCCnnnnn_n' Cuts at: 2826 3882 4928 2826 Size: 1056 1046 2826 Fragments arranged by size: 2826 1056 1046 BclI T'GATC_A Cuts at: 2448 2448 Size: 4928 BglI GCCn_nnn'nGGC Cuts at: 623 1170 1712 623 Size: 547 542 3839 Fragments arranged by size: 3839 547 542 BglII A'GATC_T Cuts at: 2443 4480 2443 Size: 2037 2891 BlpI GC'TnA_GC Cuts at: 1774 1872 2040 1774 Size: 98 168 4662 Fragments arranged by size: 4662 168 98 Bme1580I G_kGCm'C Cuts at: 152 211 1175 1197 2553 2646 3770 152 Size: 59 964 22 1356 93 1124 1310 Fragments arranged by size: 1356 1310 1124 964 93 59 22 BmrI ACTGGGnnnn_n' Cuts at: 1150 1238 1403 2140 2221 2556 4700 1150 Size: 88 165 737 81 335 2144 1378 Fragments arranged by size: 2144 1378 737 335 165 88 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3295 3295 Size: 4928 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 595 724 1276 1295 1360 595 Size: 129 552 19 65 4163 Fragments arranged by size: 4163 552 129 65 19 Bpu10I CC'TnA_GC Cuts at: 1533 1533 Size: 4928 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 346 409 493 535 2104 2983 3697 3995 346 Size: 63 84 42 1569 879 714 298 1279 Fragments arranged by size: 1569 1279 879 714 298 84 63 42 BsaI GGTCTCn'nnnn_ Cuts at: 1070 1252 1318 1391 1618 4632 1070 Size: 182 66 73 227 3014 1366 Fragments arranged by size: 3014 1366 227 182 73 66 BsaAI yAC'GTr Cuts at: 327 2281 327 Size: 1954 2974 BsaBI GATnn'nnATC Cuts at: 6 2442 4479 6 Size: 2436 2037 455 Fragments arranged by size: 2436 2037 455 BsaHI Gr'CG_yC Cuts at: 129 467 724 1729 2359 2607 3309 4805 129 Size: 338 257 1005 630 248 702 1496 252 Fragments arranged by size: 1496 1005 702 630 338 257 252 248 BsaWI w'CCGG_w Cuts at: 2117 2638 3316 3727 3874 4148 2117 Size: 521 678 411 147 274 2897 Fragments arranged by size: 2897 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 142 172 625 655 685 781 811 142 Size: 30 453 30 30 96 30 4259 Fragments arranged by size: 4259 453 96 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 532 660 663 790 907 910 1119 1122 532 Size: 128 3 127 117 3 209 3 4338 Fragments arranged by size: 4338 209 128 127 117 3 3 3 BseYI C'CCAG_C Cuts at: 603 1168 1222 1366 1402 1478 1701 2353 3111 Size: 565 54 144 36 76 223 652 758 Cuts at: 3111 3776 4184 603 Size: 665 408 1347 Fragments arranged by size: 1347 758 665 652 565 408 223 144 76 54 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 854 854 Size: 4928 BsiEI CG_ry'CG Cuts at: 104 447 2470 2516 3746 104 Size: 343 2023 46 1230 1286 Fragments arranged by size: 2023 1286 1230 343 46 BsiHKAI G_wGCw'C Cuts at: 152 1873 1942 2720 2910 3770 152 Size: 1721 69 778 190 860 1310 Fragments arranged by size: 1721 1310 860 778 190 69 BsmBI CGTCTCn'nnnn_ Cuts at: 1648 1648 Size: 4928 Bsp1286I G_dGCh'C Cuts at: 152 211 373 652 1142 1175 1197 1741 1873 Size: 59 162 279 490 33 22 544 132 Cuts at: 1873 1942 2553 2646 2720 2910 2972 3770 152 Size: 69 611 93 74 190 62 798 1310 Fragments arranged by size: 1310 798 611 544 490 279 190 162 132 93 74 69 62 59 33 22 BspHI T'CATG_A Cuts at: 3360 3360 Size: 4928 BspMI ACCTGCnnnn'nnnn_ Cuts at: 365 483 960 1978 2192 2494 2875 365 Size: 118 477 1018 214 302 381 2418 Fragments arranged by size: 2418 1018 477 381 302 214 118 BsrBI CCG'CTC Cuts at: 50 378 522 641 1773 3220 3274 4758 4923 Size: 328 144 119 1132 1447 54 1484 165 Cuts at: 4923 50 Size: 55 Fragments arranged by size: 1484 1447 1132 328 165 144 119 55 54 BsrDI GCAATG_nn' Cuts at: 201 343 2840 201 Size: 142 2497 2289 Fragments arranged by size: 2497 2289 142 BsrFI r'CCGG_y Cuts at: 1687 1695 1903 2926 3107 1687 Size: 8 208 1023 181 3508 Fragments arranged by size: 3508 1023 208 181 8 BsrGI T'GTAC_A Cuts at: 329 329 Size: 4928 BssSI C'ACGA_G Cuts at: 3199 3907 3199 Size: 708 4220 BstAPI GCAn_nnn'nTGC Cuts at: 1350 1854 2305 1350 Size: 504 451 3973 Fragments arranged by size: 3973 504 451 BstBI TT'CG_AA Cuts at: 3289 3289 Size: 4928 BstYI r'GATC_y Cuts at: 1 1033 1110 1953 2420 2443 2778 3024 3330 Size: 1032 77 843 467 23 335 246 306 Cuts at: 3330 3342 3428 4480 1 Size: 12 86 1052 449 Fragments arranged by size: 1052 1032 843 467 449 335 306 246 86 77 23 12 Bsu36I CC'TnA_GG Cuts at: 1077 1077 Size: 4928 BtgI C'CryG_G Cuts at: 821 842 991 1882 821 Size: 21 149 891 3867 Fragments arranged by size: 3867 891 149 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2878 3059 3222 2878 Size: 181 163 4584 Fragments arranged by size: 4584 181 163 BtsI GCAGTG_nn' Cuts at: 600 975 2171 600 Size: 375 1196 3357 Fragments arranged by size: 3357 1196 375 DraI TTT'AAA Cuts at: 1927 1927 Size: 4928 DraIII CAC_nnn'GTG Cuts at: 1276 1276 Size: 4928 DrdI GACnn_nn'nnGTC Cuts at: 466 1515 1564 2634 3978 466 Size: 1049 49 1070 1344 1416 Fragments arranged by size: 1416 1344 1070 1049 49 EaeI y'GGCC_r Cuts at: 824 2513 2687 3078 3105 4450 824 Size: 1689 174 391 27 1345 1302 Fragments arranged by size: 1689 1345 1302 391 174 27 EagI C'GGCC_G Cuts at: 2513 2513 Size: 4928 EarI CTCTTCn'nnn_ Cuts at: 681 728 875 2951 3161 681 Size: 47 147 2076 210 2448 Fragments arranged by size: 2448 2076 210 147 47 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3136 3878 4024 4738 3136 Size: 742 146 714 3326 Fragments arranged by size: 3326 742 714 146 EcoICRI GAG'CTC Cuts at: 1871 1940 1871 Size: 69 4859 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 594 595 724 1276 1295 1360 2753 3185 3538 Size: 1 129 552 19 65 1393 432 353 Cuts at: 3538 4200 594 Size: 662 1322 Fragments arranged by size: 1393 1322 662 552 432 353 129 65 19 1 EcoNI CCTnn'n_nnAGG Cuts at: 508 706 1146 508 Size: 198 440 4290 Fragments arranged by size: 4290 440 198 EcoO109I rG'GnC_Cy Cuts at: 207 208 797 1045 1151 1172 1194 1347 1528 Size: 1 589 248 106 21 22 153 181 Cuts at: 1528 2067 207 Size: 539 3068 Fragments arranged by size: 3068 589 539 248 181 153 106 22 21 1 EcoRI G'AATT_C Cuts at: 1932 1932 Size: 4928 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2947 2947 Size: 4928 FspI TGC'GCA Cuts at: 1684 2709 1684 Size: 1025 3903 FspAI rTGC'GCAy Cuts at: 1684 1684 Size: 4928 HaeII r_GCGC'y Cuts at: 132 1622 1702 1732 1757 2362 2610 3840 4611 Size: 1490 80 30 25 605 248 1230 771 Cuts at: 4611 132 Size: 449 Fragments arranged by size: 1490 1230 771 605 449 248 80 30 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 781 813 1048 1080 2770 2800 2802 2832 781 Size: 32 235 32 1690 30 2 30 2877 Fragments arranged by size: 2877 1690 235 32 32 30 30 2 HincII GTy'rAC Cuts at: 471 1551 1967 471 Size: 1080 416 3432 Fragments arranged by size: 3432 1080 416 KasI G'GCGC_C Cuts at: 128 1728 2358 2606 128 Size: 1600 630 248 2450 Fragments arranged by size: 2450 1600 630 248 KpnI G_GTAC'C Cuts at: 1948 1948 Size: 4928 MluI A'CGCG_T Cuts at: 4361 4361 Size: 4928 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 722 1289 3687 3871 722 Size: 567 2398 184 1779 Fragments arranged by size: 2398 1779 567 184 MscI TGG'CCA Cuts at: 826 2689 826 Size: 1863 3065 MslI CAynn'nnrTG Cuts at: 280 2917 3044 280 Size: 2637 127 2164 Fragments arranged by size: 2637 2164 127 MspA1I CmG'CkG Cuts at: 308 603 747 753 858 1789 1854 1884 2039 Size: 295 144 6 105 931 65 30 155 Cuts at: 2039 2353 2713 3497 3742 308 Size: 314 360 784 245 1494 Fragments arranged by size: 1494 931 784 360 314 295 245 155 144 105 65 30 6 NaeI GCC'GGC Cuts at: 1697 3109 1697 Size: 1412 3516 NarI GG'CG_CC Cuts at: 129 1729 2359 2607 129 Size: 1600 630 248 2450 Fragments arranged by size: 2450 1600 630 248 NcoI C'CATG_G Cuts at: 821 991 821 Size: 170 4758 NgoMIV G'CCGG_C Cuts at: 1695 3107 1695 Size: 1412 3516 NspI r_CATG'y Cuts at: 285 1981 2481 4084 285 Size: 1696 500 1603 1129 Fragments arranged by size: 1696 1603 1129 500 PasI CC'CwG_GG Cuts at: 203 1106 1142 1190 1734 203 Size: 903 36 48 544 3397 Fragments arranged by size: 3397 903 544 48 36 PciI A'CATG_T Cuts at: 4080 4080 Size: 4928 PfoI T'CCnGG_A Cuts at: 926 926 Size: 4928 PmeI GTTT'AAAC Cuts at: 1927 1927 Size: 4928 PmlI CAC'GTG Cuts at: 327 327 Size: 4928 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3374 3406 3374 Size: 32 4896 PpuMI rG'GwC_Cy Cuts at: 797 1151 1528 797 Size: 354 377 4197 Fragments arranged by size: 4197 377 354 PspOMI G'GGCC_C Cuts at: 207 1171 1193 207 Size: 964 22 3942 Fragments arranged by size: 3942 964 22 PstI C_TGCA'G Cuts at: 746 752 1056 1975 2660 746 Size: 6 304 919 685 3014 Fragments arranged by size: 3014 919 685 304 6 PvuI CG_AT'CG Cuts at: 104 104 Size: 4928 PvuII CAG'CTG Cuts at: 603 747 753 1789 2353 2713 603 Size: 144 6 1036 564 360 2818 Fragments arranged by size: 2818 1036 564 360 144 6 RsrII CG'GwC_CG Cuts at: 3123 3123 Size: 4928 SacI G_AGCT'C Cuts at: 1873 1942 1873 Size: 69 4859 SacII CC_GC'GG Cuts at: 1885 1885 Size: 4928 SalI G'TCGA_C Cuts at: 1965 1965 Size: 4928 SanDI GG'GwC_CC Cuts at: 1528 1528 Size: 4928 SapI GCTCTTCn'nnn_ Cuts at: 2951 3161 2951 Size: 210 4718 SbfI CC_TGCA'GG Cuts at: 1975 1975 Size: 4928 ScaI AGT'ACT Cuts at: 4445 4445 Size: 4928 SexAI A'CCwGG_T Cuts at: 1272 1272 Size: 4928 SfcI C'TryA_G Cuts at: 33 633 742 748 1052 1749 1971 2656 3624 Size: 600 109 6 304 697 222 685 968 Cuts at: 3624 3815 33 Size: 191 1146 Fragments arranged by size: 1146 968 697 685 600 304 222 191 109 6 SfoI GGC'GCC Cuts at: 130 1730 2360 2608 130 Size: 1600 630 248 2450 Fragments arranged by size: 2450 1600 630 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4928 SgrAI Cr'CCGG_yG Cuts at: 1695 1695 Size: 4928 SmaI CCC'GGG Cuts at: 646 1950 646 Size: 1304 3624 SmlI C'TyrA_G Cuts at: 361 424 508 550 2083 2998 3712 3974 361 Size: 63 84 42 1533 915 714 262 1315 Fragments arranged by size: 1533 1315 915 714 262 84 63 42 SpeI A'CTAG_T Cuts at: 67 67 Size: 4928 SphI G_CATG'C Cuts at: 285 1981 2481 285 Size: 1696 500 2732 Fragments arranged by size: 2732 1696 500 StyI C'CwwG_G Cuts at: 557 821 991 1240 1328 1544 2062 2416 557 Size: 264 170 249 88 216 518 354 3069 Fragments arranged by size: 3069 518 354 264 249 216 170 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2511 2644 3103 3312 2511 Size: 133 459 209 4127 Fragments arranged by size: 4127 459 209 133 TatI w'GTAC_w Cuts at: 329 437 4443 329 Size: 108 4006 814 Fragments arranged by size: 4006 814 108 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 142 174 4445 4477 142 Size: 32 4271 32 593 Fragments arranged by size: 4271 593 32 32 Tth111I GACn'n_nGTC Cuts at: 2725 2725 Size: 4928 XbaI T'CTAG_A Cuts at: 1959 1959 Size: 4928 XcmI CCAnnnn_n'nnnnTGG Cuts at: 564 930 1335 1374 2423 564 Size: 366 405 39 1049 3069 Fragments arranged by size: 3069 1049 405 366 39 XmaI C'CCGG_G Cuts at: 644 1948 644 Size: 1304 3624 XmnI GAAnn'nnTTC Cuts at: 1600 1600 Size: 4928 ZraI GAC'GTC Cuts at: 725 725 Size: 4928 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AflII AgeI AleI AlfI AloI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BspEI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PshAI PsiI PspXI PsrI SfiI SnaBI SrfI SspI StuI SwaI XhoI