Restriction map for
pF1KB1389
(Circular) (Six-base) MAPSORT of: pF1KB1389.seq Check: 428 from: 1 to: 5057
pF1KB1389 5057 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2095 2095
Size: 5057
Acc65I G'GTAC_C
Cuts at: 2073 2073
Size: 5057
AclI AA'CG_TT
Cuts at: 4897 4897
Size: 5057
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1137 1329 1713 2882 3314 3667 4329 1137
Size: 192 384 1169 432 353 662 1865
Fragments arranged by size:
1865 1169 662 432 384 353 192
AflIII A'CryG_T
Cuts at: 803 2409 4209 4490 803
Size: 1606 1800 281 1370
Fragments arranged by size:
1800 1606 1370 281
AleI CACnn'nnGTG
Cuts at: 1201 1201
Size: 5057
AlwNI CAG_nnn'CTG
Cuts at: 273 660 2362 3800 273
Size: 387 1702 1438 1530
Fragments arranged by size:
1702 1530 1438 387
ApaI G_GGCC'C
Cuts at: 324 356 1049 324
Size: 32 693 4332
Fragments arranged by size:
4332 693 32
ApaLI G'TGCA_C
Cuts at: 480 559 639 769 3895 480
Size: 79 80 130 3126 1642
Fragments arranged by size:
3126 1642 130 80 79
ApoI r'AATT_y
Cuts at: 2061 4410 4666 2061
Size: 2349 256 2452
Fragments arranged by size:
2452 2349 256
AscI GG'CGCG_CC
Cuts at: 262 262
Size: 5057
AseI AT'TA_AT
Cuts at: 20 20
Size: 5057
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5057
AvaI C'yCGr_G
Cuts at: 137 355 897 1048 2077 137
Size: 218 542 151 1029 3117
Fragments arranged by size:
3117 1029 542 218 151
BamHI G'GATC_C
Cuts at: 2082 2082
Size: 5057
BanI G'GyrC_C
Cuts at: 123 775 817 881 904 954 1297 1587 1681
Size: 652 42 64 23 50 343 290 94
Cuts at: 1681 1876 2073 2487 2735 2770 123
Size: 195 197 414 248 35 2410
Fragments arranged by size:
2410 652 414 343 290 248 197 195
94 64 50 42 35 23
BanII G_rGCy'C
Cuts at: 324 356 605 1034 1049 1903 2071 3101 324
Size: 32 249 429 15 854 168 1030 2280
Fragments arranged by size:
2280 1030 854 429 249 168 32 15
BbeI G_GCGC'C
Cuts at: 127 779 908 1591 1880 2491 2739 127
Size: 652 129 683 289 611 248 2445
Fragments arranged by size:
2445 683 652 611 289 248 129
BbsI GAAGACnn'nnnn_
Cuts at: 1244 1719 1908 1244
Size: 475 189 4393
Fragments arranged by size:
4393 475 189
BbvCI CC'TCA_GC
Cuts at: 113 389 863 113
Size: 276 474 4307
Fragments arranged by size:
4307 474 276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 471 505 471
Size: 34 5023
BciVI GTATCCnnnnn_n'
Cuts at: 2955 4011 5057 2955
Size: 1056 1046 2955
Fragments arranged by size:
2955 1056 1046
BclI T'GATC_A
Cuts at: 2577 2577
Size: 5057
BglI GCCn_nnn'nGGC
Cuts at: 196 328 1465 196
Size: 132 1137 3788
Fragments arranged by size:
3788 1137 132
BglII A'GATC_T
Cuts at: 2572 4609 2572
Size: 2037 3020
BlpI GC'TnA_GC
Cuts at: 599 1528 2169 599
Size: 929 641 3487
Fragments arranged by size:
3487 929 641
Bme1580I G_kGCm'C
Cuts at: 324 356 484 563 643 773 820 884 959
Size: 32 128 79 80 130 47 64 75
Cuts at: 959 1049 1281 1300 2682 2775 3899 324
Size: 90 232 19 1382 93 1124 1482
Fragments arranged by size:
1482 1382 1124 232 130 128 93 90
80 79 75 64 47 32 19
BmgBI CAC'GTC
Cuts at: 806 1619 1958 806
Size: 813 339 3905
Fragments arranged by size:
3905 813 339
BmrI ACTGGGnnnn_n'
Cuts at: 2269 2350 2685 4829 2269
Size: 81 335 2144 2497
Fragments arranged by size:
2497 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3424 3424
Size: 5057
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 574 1720 1846 2019 313
Size: 261 1146 126 173 3351
Fragments arranged by size:
3351 1146 261 173 126
Bpu10I CC'TnA_GC
Cuts at: 113 389 863 113
Size: 276 474 4307
Fragments arranged by size:
4307 474 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 733 934 2233 3112 3826 4124 589
Size: 144 201 1299 879 714 298 1522
Fragments arranged by size:
1522 1299 879 714 298 201 144
BsaI GGTCTCn'nnnn_
Cuts at: 4761 4761
Size: 5057
BsaAI yAC'GTr
Cuts at: 1130 2410 1130
Size: 1280 3777
BsaBI GATnn'nnATC
Cuts at: 6 2571 4608 6
Size: 2565 2037 455
Fragments arranged by size:
2565 2037 455
BsaHI Gr'CG_yC
Cuts at: 124 218 776 826 905 1588 1724 1877 2488
Size: 94 558 50 79 683 136 153 611
Cuts at: 2488 2736 3438 4934 124
Size: 248 702 1496 247
Fragments arranged by size:
1496 702 683 611 558 248 247 153
136 94 79 50
BsaWI w'CCGG_w
Cuts at: 2246 2767 3445 3856 4003 4277 2246
Size: 521 678 411 147 274 3026
Fragments arranged by size:
3026 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 133 217 1066 130
Size: 3 84 849 4121
Fragments arranged by size:
4121 849 84 3
BseYI C'CCAG_C
Cuts at: 161 309 522 1503 1814 2482 3240 3905 4313
Size: 148 213 981 311 668 758 665 408
Cuts at: 4313 161
Size: 905
Fragments arranged by size:
981 905 758 668 665 408 311 213
148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1299 607
Size: 692 4365
BsiEI CG_ry'CG
Cuts at: 104 1202 2599 2645 3875 104
Size: 1098 1397 46 1230 1286
Fragments arranged by size:
1397 1286 1230 1098 46
BsiHKAI G_wGCw'C
Cuts at: 484 563 605 622 643 773 2071 2849 3039
Size: 79 42 17 21 130 1298 778 190
Cuts at: 3039 3899 484
Size: 860 1642
Fragments arranged by size:
1642 1298 860 778 190 130 79 42
21 17
Bsp1286I G_dGCh'C
Cuts at: 324 356 484 563 605 622 643 773 820
Size: 32 128 79 42 17 21 130 47
Cuts at: 820 884 959 1034 1049 1281 1300 1903 2071
Size: 64 75 75 15 232 19 603 168
Cuts at: 2071 2682 2775 2849 3039 3101 3899 324
Size: 611 93 74 190 62 798 1482
Fragments arranged by size:
1482 798 611 603 232 190 168 130
128 93 79 75 75 74 64 62
47 42 32 21 19 17 15
BspHI T'CATG_A
Cuts at: 713 1328 1667 1919 3489 713
Size: 615 339 252 1570 2281
Fragments arranged by size:
2281 1570 615 339 252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 527 2107 2321 2623 3004 527
Size: 1580 214 302 381 2580
Fragments arranged by size:
2580 1580 381 302 214
BsrBI CCG'CTC
Cuts at: 50 404 426 674 1167 1532 3349 3403 4887
Size: 354 22 248 493 365 1817 54 1484
Cuts at: 4887 5052 50
Size: 165 55
Fragments arranged by size:
1817 1484 493 365 354 248 165 55
54 22
BsrDI GCAATG_nn'
Cuts at: 2969 2969
Size: 5057
BsrFI r'CCGG_y
Cuts at: 145 220 778 907 1222 1473 1633 3055 3236
Size: 75 558 129 315 251 160 1422 181
Cuts at: 3236 145
Size: 1966
Fragments arranged by size:
1966 1422 558 315 251 181 160 129
75
BsrGI T'GTAC_A
Cuts at: 1739 1739
Size: 5057
BssHII G'CGCG_C
Cuts at: 262 687 689 691 942 262
Size: 425 2 2 251 4377
Fragments arranged by size:
4377 425 251 2 2
BssSI C'ACGA_G
Cuts at: 1420 2013 3328 4036 1420
Size: 593 1315 708 2441
Fragments arranged by size:
2441 1315 708 593
BstAPI GCAn_nnn'nTGC
Cuts at: 660 2434 660
Size: 1774 3283
BstBI TT'CG_AA
Cuts at: 3418 3418
Size: 5057
BstXI CCAn_nnnn'nTGG
Cuts at: 522 848 522
Size: 326 4731
BstYI r'GATC_y
Cuts at: 1 1970 2082 2549 2572 2907 3153 3459 3471
Size: 1969 112 467 23 335 246 306 12
Cuts at: 3471 3557 4609 1
Size: 86 1052 449
Fragments arranged by size:
1969 1052 467 449 335 306 246 112
86 23 12
BtgI C'CryG_G
Cuts at: 107 447 885 1202 1208 107
Size: 340 438 317 6 3956
Fragments arranged by size:
3956 438 340 317 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 474 1203 1535 1749 3007 3188 3351 474
Size: 729 332 214 1258 181 163 2180
Fragments arranged by size:
2180 1258 729 332 214 181 163
BtsI GCAGTG_nn'
Cuts at: 595 2300 595
Size: 1705 3352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 436 471 436
Size: 35 5022
DraI TTT'AAA
Cuts at: 2056 2056
Size: 5057
DraIII CAC_nnn'GTG
Cuts at: 1009 1009
Size: 5057
DrdI GACnn_nn'nnGTC
Cuts at: 1912 2763 4107 1912
Size: 851 1344 2862
Fragments arranged by size:
2862 1344 851
EaeI y'GGCC_r
Cuts at: 225 736 1199 1205 1352 1457 1475 1631 2642
Size: 511 463 6 147 105 18 156 1011
Cuts at: 2642 2816 3207 3234 4579 225
Size: 174 391 27 1345 703
Fragments arranged by size:
1345 1011 703 511 463 391 174 156
147 105 27 18 6
EagI C'GGCC_G
Cuts at: 1199 2642 1199
Size: 1443 3614
EarI CTCTTCn'nnn_
Cuts at: 1120 1330 1600 1654 3080 3290 1120
Size: 210 270 54 1426 210 2887
Fragments arranged by size:
2887 1426 270 210 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 214 890 1886 3265 4007 4153 4867 214
Size: 676 996 1379 742 146 714 404
Fragments arranged by size:
1379 996 742 714 676 404 146
EcoICRI GAG'CTC
Cuts at: 603 2069 603
Size: 1466 3591
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 574 1137 1329 1713 1720 1846 2019 2882
Size: 261 563 192 384 7 126 173 863
Cuts at: 2882 3314 3667 4329 313
Size: 432 353 662 1041
Fragments arranged by size:
1041 863 662 563 432 384 353 261
192 173 126 7
EcoO109I rG'GnC_Cy
Cuts at: 352 2196 352
Size: 1844 3213
EcoRI G'AATT_C
Cuts at: 2061 2061
Size: 5057
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 744 3076 744
Size: 2332 2725
FspI TGC'GCA
Cuts at: 1717 2838 1717
Size: 1121 3936
FspAI rTGC'GCAy
Cuts at: 1717 1717
Size: 5057
HaeII r_GCGC'y
Cuts at: 127 184 661 712 779 908 1152 1504 1591
Size: 57 477 51 67 129 244 352 87
Cuts at: 1591 1880 2491 2739 3969 4740 127
Size: 289 611 248 1230 771 444
Fragments arranged by size:
1230 771 611 477 444 352 289 248
244 129 87 67 57 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2899 2929 2931 2961 2899
Size: 30 2 30 4995
Fragments arranged by size:
4995 30 30 2
HincII GTy'rAC
Cuts at: 246 2096 246
Size: 1850 3207
KasI G'GCGC_C
Cuts at: 123 775 904 1587 1876 2487 2735 123
Size: 652 129 683 289 611 248 2445
Fragments arranged by size:
2445 683 652 611 289 248 129
KpnI G_GTAC'C
Cuts at: 2077 2077
Size: 5057
MluI A'CGCG_T
Cuts at: 4490 4490
Size: 5057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 833 896 3816 4000 833
Size: 63 2920 184 1890
Fragments arranged by size:
2920 1890 184 63
MscI TGG'CCA
Cuts at: 738 1354 2818 738
Size: 616 1464 2977
Fragments arranged by size:
2977 1464 616
MslI CAynn'nnrTG
Cuts at: 1201 1666 3046 3173 1201
Size: 465 1380 127 3085
Fragments arranged by size:
3085 1380 465 127
MspA1I CmG'CkG
Cuts at: 212 270 303 477 598 1814 2168 2482 2842
Size: 58 33 174 121 1216 354 314 360
Cuts at: 2842 3626 3871 212
Size: 784 245 1398
Fragments arranged by size:
1398 1216 784 360 354 314 245 174
121 58 33
NaeI GCC'GGC
Cuts at: 147 780 909 1224 1475 1635 3238 147
Size: 633 129 315 251 160 1603 1966
Fragments arranged by size:
1966 1603 633 315 251 160 129
NarI GG'CG_CC
Cuts at: 124 776 905 1588 1877 2488 2736 124
Size: 652 129 683 289 611 248 2445
Fragments arranged by size:
2445 683 652 611 289 248 129
NcoI C'CATG_G
Cuts at: 107 107
Size: 5057
NgoMIV G'CCGG_C
Cuts at: 145 778 907 1222 1473 1633 3236 145
Size: 633 129 315 251 160 1603 1966
Fragments arranged by size:
1966 1603 633 315 251 160 129
NspI r_CATG'y
Cuts at: 1146 2110 2610 4213 1146
Size: 964 500 1603 1990
Fragments arranged by size:
1990 1603 964 500
PciI A'CATG_T
Cuts at: 4209 4209
Size: 5057
PflMI CCAn_nnn'nTGG
Cuts at: 981 1367 981
Size: 386 4671
PfoI T'CCnGG_A
Cuts at: 440 1232 1793 1996 440
Size: 792 561 203 3501
Fragments arranged by size:
3501 792 561 203
PmeI GTTT'AAAC
Cuts at: 2056 2056
Size: 5057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 592 624 3503 3535 592
Size: 32 2879 32 2114
Fragments arranged by size:
2879 2114 32 32
PshAI GACnn'nnGTC
Cuts at: 2047 2047
Size: 5057
PspOMI G'GGCC_C
Cuts at: 320 352 1045 320
Size: 32 693 4332
Fragments arranged by size:
4332 693 32
PstI C_TGCA'G
Cuts at: 2104 2789 2104
Size: 685 4372
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5057
PvuII CAG'CTG
Cuts at: 270 1814 2482 2842 270
Size: 1544 668 360 2485
Fragments arranged by size:
2485 1544 668 360
RsrII CG'GwC_CG
Cuts at: 3252 3252
Size: 5057
SacI G_AGCT'C
Cuts at: 605 2071 605
Size: 1466 3591
SalI G'TCGA_C
Cuts at: 2094 2094
Size: 5057
SapI GCTCTTCn'nnn_
Cuts at: 1120 1654 3080 3290 1120
Size: 534 1426 210 2887
Fragments arranged by size:
2887 1426 534 210
SbfI CC_TGCA'GG
Cuts at: 2104 2104
Size: 5057
ScaI AGT'ACT
Cuts at: 4574 4574
Size: 5057
SexAI A'CCwGG_T
Cuts at: 1133 1387 1133
Size: 254 4803
SfcI C'TryA_G
Cuts at: 33 2045 2100 2785 3753 3944 33
Size: 2012 55 685 968 191 1146
Fragments arranged by size:
2012 1146 968 685 191 55
SfoI GGC'GCC
Cuts at: 125 777 906 1589 1878 2489 2737 125
Size: 652 129 683 289 611 248 2445
Fragments arranged by size:
2445 683 652 611 289 248 129
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5057
SgrAI Cr'CCGG_yG
Cuts at: 145 220 778 907 145
Size: 75 558 129 4295
Fragments arranged by size:
4295 558 129 75
SmaI CCC'GGG
Cuts at: 139 357 899 2079 139
Size: 218 542 1180 3117
Fragments arranged by size:
3117 1180 542 218
SmlI C'TyrA_G
Cuts at: 604 748 949 2212 3127 3841 4103 604
Size: 144 201 1263 915 714 262 1558
Fragments arranged by size:
1558 1263 915 714 262 201 144
SnaBI TAC'GTA
Cuts at: 1130 1130
Size: 5057
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5057
SphI G_CATG'C
Cuts at: 2110 2610 2110
Size: 500 4557
SrfI GCCC'GGGC
Cuts at: 357 357
Size: 5057
StuI AGG'CCT
Cuts at: 1789 1789
Size: 5057
StyI C'CwwG_G
Cuts at: 107 1019 2191 2545 107
Size: 912 1172 354 2619
Fragments arranged by size:
2619 1172 912 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1256 1359 1705 1806 2640 2773 3232 3441 1256
Size: 103 346 101 834 133 459 209 2872
Fragments arranged by size:
2872 834 459 346 209 133 103 101
TatI w'GTAC_w
Cuts at: 413 1037 1739 4572 413
Size: 624 702 2833 898
Fragments arranged by size:
2833 898 702 624
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4574 4606 4574
Size: 32 5025
Tth111I GACn'n_nGTC
Cuts at: 2854 2854
Size: 5057
XbaI T'CTAG_A
Cuts at: 2088 2088
Size: 5057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1494 1500 2552 1494
Size: 6 1052 3999
Fragments arranged by size:
3999 1052 6
XmaI C'CCGG_G
Cuts at: 137 355 897 2077 137
Size: 218 542 1180 3117
Fragments arranged by size:
3117 1180 542 218
XmnI GAAnn'nnTTC
Cuts at: 721 721
Size: 5057
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AvrII BaeI BfrBI BmtI BsaXI BsiWI BsmI BsmBI
BspEI BstEII BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PsiI PspXI PsrI SacII
SanDI SfiI SspI SwaI XhoI ZraI