Restriction map for
pF1KB1434
(Circular) (Six-base) MAPSORT of: pF1KB1434.seq Check: 5270 from: 1 to: 9419
pF1KB1434 9419 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1820 3353 1820
Size: 1533 7886
AatII G_ACGT'C
Cuts at: 1519 1519
Size: 9419
AccI GT'mk_AC
Cuts at: 2177 6457 2177
Size: 4280 5139
Acc65I G'GTAC_C
Cuts at: 853 6435 853
Size: 5582 3837
AclI AA'CG_TT
Cuts at: 9259 9259
Size: 9419
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 483 880 1197 1858 3148 4248 4889 5426
Size: 122 397 317 661 1290 1100 641 537
Cuts at: 5426 7244 7676 8423 8691 361
Size: 1818 432 747 268 1089
Fragments arranged by size:
1818 1290 1100 1089 747 661 641 537
432 397 317 268 122
AfeI AGC'GCT
Cuts at: 3174 4084 3174
Size: 910 8509
AflIII A'CryG_T
Cuts at: 3242 5336 6771 7875 8852 3242
Size: 2094 1435 1104 977 3809
Fragments arranged by size:
3809 2094 1435 1104 977
AgeI A'CCGG_T
Cuts at: 7807 7807
Size: 9419
AhdI GACnn_n'nnGTC
Cuts at: 842 5475 842
Size: 4633 4786
AleI CACnn'nnGTG
Cuts at: 5335 5335
Size: 9419
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4594 4799 5516 6013 4594
Size: 205 717 497 8000
Fragments arranged by size:
8000 717 497 205
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3832 3864 3832
Size: 32 9387
AlwNI CAG_nnn'CTG
Cuts at: 506 1702 2836 3018 3156 5154 6724 8291 506
Size: 1196 1134 182 138 1998 1570 1567 1634
Fragments arranged by size:
1998 1634 1570 1567 1196 1134 182 138
ApaI G_GGCC'C
Cuts at: 2374 4151 2374
Size: 1777 7642
ApaLI G'TGCA_C
Cuts at: 1606 8189 1606
Size: 6583 2836
ApoI r'AATT_y
Cuts at: 2038 3895 4333 6321 6423 8772 9028 2038
Size: 1857 438 1988 102 2349 256 2429
Fragments arranged by size:
2429 2349 1988 1857 438 256 102
AseI AT'TA_AT
Cuts at: 20 2237 20
Size: 2217 7202
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9419
AvaI C'yCGr_G
Cuts at: 125 2734 4845 5819 6439 125
Size: 2609 2111 974 620 3105
Fragments arranged by size:
3105 2609 2111 974 620
BamHI G'GATC_C
Cuts at: 543 1376 6444 543
Size: 833 5068 3518
Fragments arranged by size:
5068 3518 833
BanI G'GyrC_C
Cuts at: 475 853 1832 3321 3589 4944 5576 6435 6849
Size: 378 979 1489 268 1355 632 859 414
Cuts at: 6849 7097 7132 475
Size: 248 35 2762
Fragments arranged by size:
2762 1489 1355 979 859 632 414 378
268 248 35
BanII G_rGCy'C
Cuts at: 1349 1396 2015 2374 2542 2699 4151 4543 4925
Size: 47 619 359 168 157 1452 392 382
Cuts at: 4925 6433 7463 1349
Size: 1508 1030 3305
Fragments arranged by size:
3305 1508 1452 1030 619 392 382 359
168 157 47
BbeI G_GCGC'C
Cuts at: 4948 6853 7101 4948
Size: 1905 248 7266
Fragments arranged by size:
7266 1905 248
BbsI GAAGACnn'nnnn_
Cuts at: 418 3136 4319 5188 418
Size: 2718 1183 869 4649
Fragments arranged by size:
4649 2718 1183 869
BbvCI CC'TCA_GC
Cuts at: 3665 6355 3665
Size: 2690 6729
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 967 1001 1450 1484 4894 4928 967
Size: 34 449 34 3410 34 5458
Fragments arranged by size:
5458 3410 449 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7317 8078 9419 7317
Size: 761 1341 7317
Fragments arranged by size:
7317 1341 761
BclI T'GATC_A
Cuts at: 6113 6939 6113
Size: 826 8593
BglI GCCn_nnn'nGGC
Cuts at: 112 2383 112
Size: 2271 7148
BglII A'GATC_T
Cuts at: 4061 4238 4693 5630 6934 8971 4061
Size: 177 455 937 1304 2037 4509
Fragments arranged by size:
4509 2037 1304 937 455 177
BlpI GC'TnA_GC
Cuts at: 1390 2468 2684 3481 4924 6531 1390
Size: 1078 216 797 1443 1607 4278
Fragments arranged by size:
4278 1607 1443 1078 797 216
Bme1580I G_kGCm'C
Cuts at: 480 1610 2374 3324 4151 7044 7137 8193 480
Size: 1130 764 950 827 2893 93 1056 1706
Fragments arranged by size:
2893 1706 1130 1056 950 827 764 93
BmrI ACTGGGnnnn_n'
Cuts at: 920 1181 1648 1855 3384 6631 6712 7047 9191
Size: 261 467 207 1529 3247 81 335 2144
Cuts at: 9191 920
Size: 1148
Fragments arranged by size:
3247 2144 1529 1148 467 335 261 207
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 496 4083 4775 7786 496
Size: 3587 692 3011 2129
Fragments arranged by size:
3587 3011 2129 692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 956 1008 1333 1339 2353 2626 2824 3019
Size: 794 52 325 6 1014 273 198 195
Cuts at: 3019 3132 3286 4053 4710 4770 4901 4965 162
Size: 113 154 767 657 60 131 64 4616
Fragments arranged by size:
4616 1014 794 767 657 325 273 198
195 154 131 113 64 60 52 6
Bpu10I CC'TnA_GC
Cuts at: 2541 3665 6355 2541
Size: 1124 2690 5605
Fragments arranged by size:
5605 2690 1124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2007 4274 6595 7474 7966 8264 2007
Size: 2267 2321 879 492 298 3162
Fragments arranged by size:
3162 2321 2267 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 237 9123 237
Size: 8886 533
BsaAI yAC'GTr
Cuts at: 3245 4134 6772 3245
Size: 889 2638 5892
Fragments arranged by size:
5892 2638 889
BsaBI GATnn'nnATC
Cuts at: 6 673 2837 6933 8970 6
Size: 667 2164 4096 2037 455
Fragments arranged by size:
4096 2164 2037 667 455
BsaHI Gr'CG_yC
Cuts at: 1516 3215 4907 4945 5051 6850 7098 7800 9296
Size: 1699 1692 38 106 1799 248 702 1496
Cuts at: 9296 1516
Size: 1639
Fragments arranged by size:
1799 1699 1692 1639 1496 702 248 106
38
BsaWI w'CCGG_w
Cuts at: 1373 2104 3224 3764 5046 6608 7129 7807 8081
Size: 731 1120 540 1282 1562 521 678 274
Cuts at: 8081 8228 8639 1373
Size: 147 411 2153
Fragments arranged by size:
2153 1562 1282 1120 731 678 540 521
411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 796 826 3827 3857 4525 4555 5800 5830 796
Size: 30 3001 30 668 30 1245 30 4385
Fragments arranged by size:
4385 3001 1245 668 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 709 737 1144 2776 3004 3007 5272 709
Size: 28 407 1632 228 3 2265 4856
Fragments arranged by size:
4856 2265 1632 407 228 28 3
BseYI C'CCAG_C
Cuts at: 823 2085 2325 2614 3392 3752 3806 4544 4940
Size: 1262 240 289 778 360 54 738 396
Cuts at: 4940 5147 5283 5437 5829 6074 6844 7602 8179
Size: 207 136 154 392 245 770 758 577
Cuts at: 8179 8675 823
Size: 496 1567
Fragments arranged by size:
1567 1262 778 770 758 738 577 496
396 392 360 289 245 240 207 154
136 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1490 2341 2417 3266 4036 4534 4795 5101
Size: 1231 851 76 849 770 498 261 306
Cuts at: 5101 5437 5622 5796 259
Size: 336 185 174 3882
Fragments arranged by size:
3882 1231 851 849 770 498 336 306
261 185 174 76
BsiEI CG_ry'CG
Cuts at: 104 6209 6961 7007 8215 104
Size: 6105 752 46 1208 1308
Fragments arranged by size:
6105 1308 1208 752 46
BsiHKAI G_wGCw'C
Cuts at: 1349 1610 2279 2438 2699 4925 6433 7211 7401
Size: 261 669 159 261 2226 1508 778 190
Cuts at: 7401 8193 1349
Size: 792 2575
Fragments arranged by size:
2575 2226 1508 792 778 669 261 261
190 159
BsiWI C'GTAC_G
Cuts at: 3245 3245
Size: 9419
BsmI GAATG_Cn'
Cuts at: 1425 1737 1425
Size: 312 9107
BsmBI CGTCTCn'nnnn_
Cuts at: 3784 4618 4834 5657 6216 3784
Size: 834 216 823 559 6987
Fragments arranged by size:
6987 834 823 559 216
Bsp1286I G_dGCh'C
Cuts at: 480 1349 1396 1610 2015 2279 2374 2438 2542
Size: 869 47 214 405 264 95 64 104
Cuts at: 2542 2699 3324 4151 4543 4925 6433 7044 7137
Size: 157 625 827 392 382 1508 611 93
Cuts at: 7137 7211 7401 7463 8193 480
Size: 74 190 62 730 1706
Fragments arranged by size:
1706 1508 869 827 730 625 611 405
392 382 264 214 190 157 104 95
93 74 64 62 47
BspEI T'CCGG_A
Cuts at: 3764 5046 8639 3764
Size: 1282 3593 4544
Fragments arranged by size:
4544 3593 1282
BspHI T'CATG_A
Cuts at: 2297 4325 6329 8595 2297
Size: 2028 2004 2266 3121
Fragments arranged by size:
3121 2266 2028 2004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 573 1820 2726 3353 5441 6469 6683 6985 7366
Size: 1247 906 627 2088 1028 214 302 381
Cuts at: 7366 573
Size: 2626
Fragments arranged by size:
2626 2088 1247 1028 906 627 381 302
214
BsrBI CCG'CTC
Cuts at: 50 227 812 3003 3116 5582 7711 7765 9249
Size: 177 585 2191 113 2466 2129 54 1484
Cuts at: 9249 9414 50
Size: 165 55
Fragments arranged by size:
2466 2191 2129 1484 585 177 165 113
55 54
BsrDI GCAATG_nn'
Cuts at: 1713 1973 2217 3707 4227 7331 1713
Size: 260 244 1490 520 3104 3801
Fragments arranged by size:
3801 3104 1490 520 260 244
BsrFI r'CCGG_y
Cuts at: 1898 2798 4127 5672 7417 7598 7807 1898
Size: 900 1329 1545 1745 181 209 3510
Fragments arranged by size:
3510 1745 1545 1329 900 209 181
BssSI C'ACGA_G
Cuts at: 1332 7690 8048 1332
Size: 6358 358 2703
Fragments arranged by size:
6358 2703 358
BstAPI GCAn_nnn'nTGC
Cuts at: 343 588 5682 6796 343
Size: 245 5094 1114 2966
Fragments arranged by size:
5094 2966 1114 245
BstBI TT'CG_AA
Cuts at: 786 7780 786
Size: 6994 2425
BstEII G'GTnAC_C
Cuts at: 2673 4887 2673
Size: 2214 7205
BstXI CCAn_nnnn'nTGG
Cuts at: 1173 2561 3525 5460 1173
Size: 1388 964 1935 5132
Fragments arranged by size:
5132 1935 1388 964
BstYI r'GATC_y
Cuts at: 1 305 543 940 1301 1376 2024 2832 3633
Size: 304 238 397 361 75 648 808 801
Cuts at: 3633 4061 4238 4693 5630 6444 6911 6934 7269
Size: 428 177 455 937 814 467 23 335
Cuts at: 7269 7515 8527 8613 8625 8971 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 937 814 808 801 648 467 455
449 428 397 361 346 335 304 246
238 177 86 75 23 12
BstZ17I GTA'TAC
Cuts at: 2178 2178
Size: 9419
Bsu36I CC'TnA_GG
Cuts at: 5089 5447 5089
Size: 358 9061
BtgI C'CryG_G
Cuts at: 107 300 2205 3410 4949 107
Size: 193 1905 1205 1539 4577
Fragments arranged by size:
4577 1905 1539 1205 193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1794 2238 5553 7369 7550 7713 1794
Size: 444 3315 1816 181 163 3500
Fragments arranged by size:
3500 3315 1816 444 181 163
BtsI GCAGTG_nn'
Cuts at: 344 882 2237 2400 2626 2914 4247 4729 5771
Size: 538 1355 163 226 288 1333 482 1042
Cuts at: 5771 6662 344
Size: 891 3101
Fragments arranged by size:
3101 1355 1333 1042 891 538 482 288
226 163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4236 4271 4236
Size: 35 9384
DraI TTT'AAA
Cuts at: 6418 6418
Size: 9419
DraIII CAC_nnn'GTG
Cuts at: 1614 2283 3289 1614
Size: 669 1006 7744
Fragments arranged by size:
7744 1006 669
DrdI GACnn_nn'nnGTC
Cuts at: 1639 4429 6208 7125 7983 1639
Size: 2790 1779 917 858 3075
Fragments arranged by size:
3075 2790 1779 917 858
EaeI y'GGCC_r
Cuts at: 1883 2246 2258 2551 3407 3413 4139 4595 4682
Size: 363 12 293 856 6 726 456 87
Cuts at: 4682 5519 5622 5768 5936 6258 7004 7178 7569
Size: 837 103 146 168 322 746 174 391
Cuts at: 7569 7596 8941 1883
Size: 27 1345 2361
Fragments arranged by size:
2361 1345 856 837 746 726 456 391
363 322 293 174 168 146 103 87
27 12 6
EagI C'GGCC_G
Cuts at: 7004 7004
Size: 9419
EarI CTCTTCn'nnn_
Cuts at: 704 764 856 1768 2146 2494 5065 5198 5713
Size: 60 92 912 378 348 2571 133 515
Cuts at: 5713 7442 7652 704
Size: 1729 210 2471
Fragments arranged by size:
2571 2471 1729 912 515 378 348 210
133 92 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 149 155 161 2977 7627 7937 8083 9229 149
Size: 6 6 2816 4650 310 146 1146 339
Fragments arranged by size:
4650 2816 1146 339 310 146 6 6
EcoICRI GAG'CTC
Cuts at: 1347 2697 4923 6431 1347
Size: 1350 2226 1508 4335
Fragments arranged by size:
4335 2226 1508 1350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 361 483 880 956 1008 1197 1333 1339
Size: 199 122 397 76 52 189 136 6
Cuts at: 1339 1858 2353 2626 2824 3019 3132 3148 3286
Size: 519 495 273 198 195 113 16 138
Cuts at: 3286 4053 4248 4710 4770 4889 4901 4965 5426
Size: 767 195 462 60 119 12 64 461
Cuts at: 5426 7244 7676 8423 8691 162
Size: 1818 432 747 268 890
Fragments arranged by size:
1818 890 767 747 519 495 462 461
432 397 273 268 199 198 195 195
189 138 136 122 119 113 76 64
60 52 16 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3304 4289 3304
Size: 985 8434
EcoO109I rG'GnC_Cy
Cuts at: 1275 2370 2371 3757 3869 4148 5085 5483 6558
Size: 1095 1 1386 112 279 937 398 1075
Cuts at: 6558 1275
Size: 4136
Fragments arranged by size:
4136 1386 1095 1075 937 398 279 112
1
EcoRI G'AATT_C
Cuts at: 6423 6423
Size: 9419
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 658 2616 2781 5736 7438 658
Size: 1958 165 2955 1702 2639
Fragments arranged by size:
2955 2639 1958 1702 165
FspI TGC'GCA
Cuts at: 3544 7200 3544
Size: 3656 5763
HaeII r_GCGC'y
Cuts at: 185 3176 3492 4086 4948 6853 7101 8123 9102
Size: 2991 316 594 862 1905 248 1022 979
Cuts at: 9102 185
Size: 502
Fragments arranged by size:
2991 1905 1022 979 862 594 502 316
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 796 828 860 1545 1577 4596 4628 5461 5493
Size: 32 32 685 32 3019 32 833 32
Cuts at: 5493 5800 5832 7261 7291 7293 7323 796
Size: 307 32 1429 30 2 30 2892
Fragments arranged by size:
3019 2892 1429 833 685 307 32 32
32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1748 3958 6458 1748
Size: 2210 2500 4709
Fragments arranged by size:
4709 2500 2210
HindIII A'AGCT_T
Cuts at: 271 271
Size: 9419
KasI G'GCGC_C
Cuts at: 4944 6849 7097 4944
Size: 1905 248 7266
Fragments arranged by size:
7266 1905 248
KpnI G_GTAC'C
Cuts at: 857 6439 857
Size: 5582 3837
MluI A'CGCG_T
Cuts at: 8852 8852
Size: 9419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1919 2288 3542 4820 6212 8090 8274 1919
Size: 369 1254 1278 1392 1878 184 3064
Fragments arranged by size:
3064 1878 1392 1278 1254 369 184
MscI TGG'CCA
Cuts at: 1885 2248 2553 3409 3415 4597 4684 5521 5770
Size: 363 305 856 6 1182 87 837 249
Cuts at: 5770 7180 1885
Size: 1410 4124
Fragments arranged by size:
4124 1410 1182 856 837 363 305 249
87 6
MslI CAynn'nnrTG
Cuts at: 1978 3883 4193 5335 6160 6226 7408 7535 1978
Size: 1905 310 1142 825 66 1182 127 3862
Fragments arranged by size:
3862 1905 1182 1142 825 310 127 66
MspA1I CmG'CkG
Cuts at: 2085 2325 2683 3480 4020 4268 4487 4548 4635
Size: 240 358 797 540 248 219 61 87
Cuts at: 4635 4779 5304 5727 5829 6530 6844 7204 8217
Size: 144 525 423 102 701 314 360 1013
Cuts at: 8217 8462 2085
Size: 245 3042
Fragments arranged by size:
3042 1013 797 701 540 525 423 360
358 314 248 245 240 219 144 102
87 61
NaeI GCC'GGC
Cuts at: 5674 7600 5674
Size: 1926 7493
NarI GG'CG_CC
Cuts at: 4945 6850 7098 4945
Size: 1905 248 7266
Fragments arranged by size:
7266 1905 248
NcoI C'CATG_G
Cuts at: 107 300 3410 107
Size: 193 3110 6116
Fragments arranged by size:
6116 3110 193
NgoMIV G'CCGG_C
Cuts at: 5672 7598 5672
Size: 1926 7493
NruI TCG'CGA
Cuts at: 681 4232 681
Size: 3551 5868
NspI r_CATG'y
Cuts at: 285 595 918 4192 4494 4521 4989 5685 5856
Size: 310 323 3274 302 27 468 696 171
Cuts at: 5856 6472 6972 7879 285
Size: 616 500 907 1825
Fragments arranged by size:
3274 1825 907 696 616 500 468 323
310 302 171 27
PasI CC'CwG_GG
Cuts at: 4170 5779 4170
Size: 1609 7810
PciI A'CATG_T
Cuts at: 7875 7875
Size: 9419
PflMI CCAn_nnn'nTGG
Cuts at: 618 3317 5154 618
Size: 2699 1837 4883
Fragments arranged by size:
4883 2699 1837
PfoI T'CCnGG_A
Cuts at: 764 3302 3629 5240 764
Size: 2538 327 1611 4943
Fragments arranged by size:
4943 2538 1611 327
PmeI GTTT'AAAC
Cuts at: 6418 6418
Size: 9419
PmlI CAC'GTG
Cuts at: 4134 4134
Size: 9419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8558 8590 8558
Size: 32 9387
PpuMI rG'GwC_Cy
Cuts at: 1275 3757 5483 1275
Size: 2482 1726 5211
Fragments arranged by size:
5211 2482 1726
PshAI GACnn'nnGTC
Cuts at: 3656 3656
Size: 9419
PspOMI G'GGCC_C
Cuts at: 2370 4147 2370
Size: 1777 7642
PstI C_TGCA'G
Cuts at: 1508 1568 1831 2024 3152 3350 4045 4082 5696
Size: 60 263 193 1128 198 695 37 1614
Cuts at: 5696 5969 6466 7151 1508
Size: 273 497 685 3776
Fragments arranged by size:
3776 1614 1128 695 685 497 273 263
198 193 60 37
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9419
PvuII CAG'CTG
Cuts at: 2085 2325 3480 4268 4548 4779 5727 5829 6844
Size: 240 1155 788 280 231 948 102 1015
Cuts at: 6844 7204 2085
Size: 360 4300
Fragments arranged by size:
4300 1155 1015 948 788 360 280 240
231 102
RsrII CG'GwC_CG
Cuts at: 2633 7614 2633
Size: 4981 4438
SacI G_AGCT'C
Cuts at: 1349 2699 4925 6433 1349
Size: 1350 2226 1508 4335
Fragments arranged by size:
4335 2226 1508 1350
SalI G'TCGA_C
Cuts at: 6456 6456
Size: 9419
SanDI GG'GwC_CC
Cuts at: 3757 5483 3757
Size: 1726 7693
SapI GCTCTTCn'nnn_
Cuts at: 764 1768 2494 7442 7652 764
Size: 1004 726 4948 210 2531
Fragments arranged by size:
4948 2531 1004 726 210
SbfI CC_TGCA'GG
Cuts at: 3350 6466 3350
Size: 3116 6303
ScaI AGT'ACT
Cuts at: 1003 2414 4039 6391 8936 1003
Size: 1411 1625 2352 2545 1486
Fragments arranged by size:
2545 2352 1625 1486 1411
SexAI A'CCwGG_T
Cuts at: 1109 2279 1109
Size: 1170 8249
SfcI C'TryA_G
Cuts at: 33 537 1504 1564 1827 2020 2029 2211 3148
Size: 504 967 60 263 193 9 182 937
Cuts at: 3148 3346 4041 4078 5692 5943 5965 6462 7147
Size: 198 695 37 1614 251 22 497 685
Cuts at: 7147 8140 8331 33
Size: 993 191 1121
Fragments arranged by size:
1614 1121 993 967 937 695 685 504
497 263 251 198 193 191 182 60
37 22 9
SfoI GGC'GCC
Cuts at: 4946 6851 7099 4946
Size: 1905 248 7266
Fragments arranged by size:
7266 1905 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9419
SmaI CCC'GGG
Cuts at: 127 4847 5821 6441 127
Size: 4720 974 620 3105
Fragments arranged by size:
4720 3105 974 620
SmlI C'TyrA_G
Cuts at: 1986 4289 6574 7489 7981 8243 1986
Size: 2303 2285 915 492 262 3162
Fragments arranged by size:
3162 2303 2285 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9419
SphI G_CATG'C
Cuts at: 918 4192 6472 6972 918
Size: 3274 2280 500 3365
Fragments arranged by size:
3365 3274 2280 500
StuI AGG'CCT
Cuts at: 616 4967 616
Size: 4351 5068
StyI C'CwwG_G
Cuts at: 107 254 300 617 1964 2978 3410 4112 4455
Size: 147 46 317 1347 1014 432 702 343
Cuts at: 4455 6553 6907 107
Size: 2098 354 2619
Fragments arranged by size:
2619 2098 1347 1014 702 432 354 343
317 147 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2469 4468 4974 5771 7002 7135 7594 7803 2469
Size: 1999 506 797 1231 133 459 209 4085
Fragments arranged by size:
4085 1999 1231 797 506 459 209 133
TatI w'GTAC_w
Cuts at: 1001 1262 2412 2807 4037 4700 6389 8934 1001
Size: 261 1150 395 1230 663 1689 2545 1486
Fragments arranged by size:
2545 1689 1486 1230 1150 663 395 261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1838 1870 2516 2548 3827 3859 8936 8968 1838
Size: 32 646 32 1279 32 5077 32 2289
Fragments arranged by size:
5077 2289 1279 646 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1620 2671 3156 6205 7216 1620
Size: 1051 485 3049 1011 3823
Fragments arranged by size:
3823 3049 1051 1011 485
XbaI T'CTAG_A
Cuts at: 5196 6450 5196
Size: 1254 8165
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 437 1699 5655 6031 6914 437
Size: 1262 3956 376 883 2942
Fragments arranged by size:
3956 2942 1262 883 376
XmaI C'CCGG_G
Cuts at: 125 4845 5819 6439 125
Size: 4720 974 620 3105
Fragments arranged by size:
4720 3105 974 620
XmnI GAAnn'nnTTC
Cuts at: 782 792 1091 2140 2488 3274 5719 782
Size: 10 299 1049 348 786 2445 4482
Fragments arranged by size:
4482 2445 1049 786 348 299 10
ZraI GAC'GTC
Cuts at: 1517 1517
Size: 9419
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BfrBI BmgBI BmtI BsrGI
BssHII ClaI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NsiI PacI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI