Restriction map for
pF1KB1440
(Circular) (Six-base) MAPSORT of: pF1KB1440.seq Check: 5754 from: 1 to: 8807
pF1KB1440 8807 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5532 5532
Size: 8807
AatII G_ACGT'C
Cuts at: 2402 3160 2402
Size: 758 8049
AccI GT'mk_AC
Cuts at: 632 1454 2288 2869 5675 5845 632
Size: 822 834 581 2806 170 3594
Fragments arranged by size:
3594 2806 834 822 581 170
Acc65I G'GTAC_C
Cuts at: 3489 5823 3489
Size: 2334 6473
AclI AA'CG_TT
Cuts at: 8647 8647
Size: 8807
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1843 3649 4006 6632 7064 7811 8079 556
Size: 1287 1806 357 2626 432 747 268 1284
Fragments arranged by size:
2626 1806 1287 1284 747 432 357 268
AfeI AGC'GCT
Cuts at: 337 3787 5350 337
Size: 3450 1563 3794
Fragments arranged by size:
3794 3450 1563
AflIII A'CryG_T
Cuts at: 751 4871 5036 5678 6159 7263 8240 751
Size: 4120 165 642 481 1104 977 1318
Fragments arranged by size:
4120 1318 1104 977 642 481 165
AgeI A'CCGG_T
Cuts at: 7195 7195
Size: 8807
AleI CACnn'nnGTG
Cuts at: 1000 1528 1585 2173 2278 2350 2938 4571 4921
Size: 528 57 588 105 72 588 1633 350
Cuts at: 4921 5597 5620 1000
Size: 676 23 4187
Fragments arranged by size:
4187 1633 676 588 588 528 350 105
72 57 23
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1558 2218 3137 5615 1558
Size: 660 919 2478 4750
Fragments arranged by size:
4750 2478 919 660
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4990 5022 4990
Size: 32 8775
AlwNI CAG_nnn'CTG
Cuts at: 598 766 2970 3348 4959 6112 7679 598
Size: 168 2204 378 1611 1153 1567 1726
Fragments arranged by size:
2204 1726 1611 1567 1153 378 168
ApaI G_GGCC'C
Cuts at: 1192 2182 4357 4709 1192
Size: 990 2175 352 5290
Fragments arranged by size:
5290 2175 990 352
ApaLI G'TGCA_C
Cuts at: 878 4717 5590 7577 878
Size: 3839 873 1987 2108
Fragments arranged by size:
3839 2108 1987 873
ApoI r'AATT_y
Cuts at: 4185 5811 8160 8416 4185
Size: 1626 2349 256 4576
Fragments arranged by size:
4576 2349 1626 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8807
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8807
AvaI C'yCGr_G
Cuts at: 1417 2188 2812 3541 4250 4282 4363 4448 5002
Size: 771 624 729 709 32 81 85 554
Cuts at: 5002 5441 5827 1417
Size: 439 386 4397
Fragments arranged by size:
4397 771 729 709 624 554 439 386
85 81 32
BamHI G'GATC_C
Cuts at: 383 1688 5832 383
Size: 1305 4144 3358
Fragments arranged by size:
4144 3358 1305
BanI G'GyrC_C
Cuts at: 141 184 676 1341 2497 2600 3489 4483 4923
Size: 43 492 665 1156 103 889 994 440
Cuts at: 4923 5823 6237 6485 6520 141
Size: 900 414 248 35 2428
Fragments arranged by size:
2428 1156 994 900 889 665 492 440
414 248 103 43 35
BanII G_rGCy'C
Cuts at: 923 968 1192 1427 1520 1966 2182 2375 4357
Size: 45 224 235 93 446 216 193 1982
Cuts at: 4357 4709 5003 5155 5821 6851 923
Size: 352 294 152 666 1030 2879
Fragments arranged by size:
2879 1982 1030 666 446 352 294 235
224 216 193 152 93 45
BbeI G_GCGC'C
Cuts at: 188 1345 6241 6489 188
Size: 1157 4896 248 2506
Fragments arranged by size:
4896 2506 1157 248
BbsI GAAGACnn'nnnn_
Cuts at: 201 1772 1875 3160 4801 5701 201
Size: 1571 103 1285 1641 900 3307
Fragments arranged by size:
3307 1641 1571 1285 900 103
BbvCI CC'TCA_GC
Cuts at: 530 1243 530
Size: 713 8094
BciVI GTATCCnnnnn_n'
Cuts at: 1438 6705 7466 8807 1438
Size: 5267 761 1341 1438
Fragments arranged by size:
5267 1438 1341 761
BclI T'GATC_A
Cuts at: 2561 4461 6327 2561
Size: 1900 1866 5041
Fragments arranged by size:
5041 1900 1866
BglI GCCn_nnn'nGGC
Cuts at: 2015 2047 2235 2665 4745 2015
Size: 32 188 430 2080 6077
Fragments arranged by size:
6077 2080 430 188 32
BglII A'GATC_T
Cuts at: 6322 8359 6322
Size: 2037 6770
BlpI GC'TnA_GC
Cuts at: 967 1381 3169 5919 967
Size: 414 1788 2750 3855
Fragments arranged by size:
3855 2750 1788 414
Bme1580I G_kGCm'C
Cuts at: 146 882 1183 1192 1326 2166 2182 2231 2502
Size: 736 301 9 134 840 16 49 271
Cuts at: 2502 3209 3254 4357 4709 4721 5507 5594 6432
Size: 707 45 1103 352 12 786 87 838
Cuts at: 6432 6525 7581 146
Size: 93 1056 1372
Fragments arranged by size:
1372 1103 1056 840 838 786 736 707
352 301 271 134 93 87 49 45
16 12 9
BmgBI CAC'GTC
Cuts at: 2175 2226 2867 5039 2175
Size: 51 641 2172 5943
Fragments arranged by size:
5943 2172 641 51
BmrI ACTGGGnnnn_n'
Cuts at: 245 1758 1908 2022 5400 6019 6100 6435 8579
Size: 1513 150 114 3378 619 81 335 2144
Cuts at: 8579 245
Size: 473
Fragments arranged by size:
3378 2144 1513 619 473 335 150 114
81
BmtI G_CTAG'C
Cuts at: 2410 2410
Size: 8807
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7174 7174
Size: 8807
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1332 2091 2149 2380 2578 3571 3780 4216
Size: 350 759 58 231 198 993 209 436
Cuts at: 4216 4404 4456 4563 4915 4958 5668 5788 982
Size: 188 52 107 352 43 710 120 4001
Fragments arranged by size:
4001 993 759 710 436 352 350 231
209 198 188 120 107 58 52 43
Bpu10I CC'TnA_GC
Cuts at: 116 530 1243 116
Size: 414 713 7680
Fragments arranged by size:
7680 713 414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2998 3753 4114 5983 6862 7354 7652 2998
Size: 755 361 1869 879 492 298 4153
Fragments arranged by size:
4153 1869 879 755 492 361 298
BsaI GGTCTCn'nnnn_
Cuts at: 2443 4055 4360 4483 8511 2443
Size: 1612 305 123 4028 2739
Fragments arranged by size:
4028 2739 1612 305 123
BsaAI yAC'GTr
Cuts at: 894 2352 4178 4818 4872 4907 6160 894
Size: 1458 1826 640 54 35 1253 3541
Fragments arranged by size:
3541 1826 1458 1253 640 54 35
BsaBI GATnn'nnATC
Cuts at: 6 6321 8358 6
Size: 6315 2037 455
Fragments arranged by size:
6315 2037 455
BsaHI Gr'CG_yC
Cuts at: 185 1342 1880 2399 3157 3523 4277 6238 6486
Size: 1157 538 519 758 366 754 1961 248
Cuts at: 6486 7188 8684 185
Size: 702 1496 308
Fragments arranged by size:
1961 1496 1157 758 754 702 538 519
366 308 248
BsaWI w'CCGG_w
Cuts at: 1464 3726 5996 6517 7195 7469 7616 8027 1464
Size: 2262 2270 521 678 274 147 411 2244
Fragments arranged by size:
2270 2262 2244 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1783 1813 3103 3133 3850 3880 3936 3966 4135
Size: 30 1290 30 717 30 56 30 169
Cuts at: 4135 4165 4429 4459 4852 4882 4990 5020 1783
Size: 30 264 30 393 30 108 30 5570
Fragments arranged by size:
5570 1290 717 393 264 169 108 56
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 791 931 1002 1435 1510 1791 2800 3607 3865
Size: 140 71 433 75 281 1009 807 258
Cuts at: 3865 3999 4453 791
Size: 134 454 5145
Fragments arranged by size:
5145 1009 807 454 433 281 258 140
134 75 71
BseYI C'CCAG_C
Cuts at: 332 820 1017 1306 1603 1646 2111 2230 2308
Size: 488 197 289 297 43 465 119 78
Cuts at: 2308 2631 2824 3082 3173 3839 4959 6232 6990
Size: 323 193 258 91 666 1120 1273 758
Cuts at: 6990 7567 8063 332
Size: 577 496 1076
Fragments arranged by size:
1273 1120 1076 758 666 577 496 488
465 323 297 289 258 197 193 119
91 78 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 421 1387 1423 1841 2555 2710 4873 5746
Size: 281 966 36 418 714 155 2163 873
Cuts at: 5746 140
Size: 3201
Fragments arranged by size:
3201 2163 966 873 714 418 281 155
36
BsiEI CG_ry'CG
Cuts at: 104 654 1529 3423 3980 6349 6395 7603 104
Size: 550 875 1894 557 2369 46 1208 1308
Fragments arranged by size:
2369 1894 1308 1208 875 557 550 46
BsiHKAI G_wGCw'C
Cuts at: 482 882 923 968 2105 3538 3662 4721 5003
Size: 400 41 45 1137 1433 124 1059 282
Cuts at: 5003 5239 5270 5594 5821 6599 6789 7581 482
Size: 236 31 324 227 778 190 792 1708
Fragments arranged by size:
1708 1433 1137 1059 792 778 400 324
282 236 227 190 124 45 41 31
BsiWI C'GTAC_G
Cuts at: 978 978
Size: 8807
BsmI GAATG_Cn'
Cuts at: 4338 4338
Size: 8807
Bsp1286I G_dGCh'C
Cuts at: 146 482 882 923 968 1183 1192 1326 1427
Size: 336 400 41 45 215 9 134 101
Cuts at: 1427 1520 1966 2105 2166 2182 2231 2375 2502
Size: 93 446 139 61 16 49 144 127
Cuts at: 2502 3209 3254 3538 3662 4357 4709 4721 5003
Size: 707 45 284 124 695 352 12 282
Cuts at: 5003 5155 5239 5270 5507 5594 5821 6432 6525
Size: 152 84 31 237 87 227 611 93
Cuts at: 6525 6599 6789 6851 7581 146
Size: 74 190 62 730 1372
Fragments arranged by size:
1372 730 707 695 611 446 400 352
336 284 282 237 227 215 190 152
144 139 134 127 124 101 93 93
87 84 74 62 61 49 45 45
41 31 16 12 9
BspEI T'CCGG_A
Cuts at: 1464 3726 8027 1464
Size: 2262 4301 2244
Fragments arranged by size:
4301 2262 2244
BspHI T'CATG_A
Cuts at: 4421 7983 4421
Size: 3562 5245
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2828 2852 3287 4831 5069 5532 5857 6071 6373
Size: 24 435 1544 238 463 325 214 302
Cuts at: 6373 6754 2828
Size: 381 4881
Fragments arranged by size:
4881 1544 463 435 381 325 302 238
214 24
BsrBI CCG'CTC
Cuts at: 50 1722 4779 7099 7153 8637 8802 50
Size: 1672 3057 2320 54 1484 165 55
Fragments arranged by size:
3057 2320 1672 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 391 978 3155 6719 391
Size: 587 2177 3564 2479
Fragments arranged by size:
3564 2479 2177 587
BsrFI r'CCGG_y
Cuts at: 2546 2639 2719 3105 3713 4744 5084 6805 6986
Size: 93 80 386 608 1031 340 1721 181
Cuts at: 6986 7195 2546
Size: 209 4158
Fragments arranged by size:
4158 1721 1031 608 386 340 209 181
93 80
BsrGI T'GTAC_A
Cuts at: 427 3221 427
Size: 2794 6013
BssSI C'ACGA_G
Cuts at: 642 1698 4789 7078 7436 642
Size: 1056 3091 2289 358 2013
Fragments arranged by size:
3091 2289 2013 1056 358
BstAPI GCAn_nnn'nTGC
Cuts at: 877 1638 3258 4748 5502 6184 877
Size: 761 1620 1490 754 682 3500
Fragments arranged by size:
3500 1620 1490 761 754 682
BstBI TT'CG_AA
Cuts at: 7168 7168
Size: 8807
BstEII G'GTnAC_C
Cuts at: 1782 2343 4509 1782
Size: 561 2166 6080
Fragments arranged by size:
6080 2166 561
BstXI CCAn_nnnn'nTGG
Cuts at: 522 1434 2880 3108 4573 5178 522
Size: 912 1446 228 1465 605 4151
Fragments arranged by size:
4151 1465 1446 912 605 228
BstYI r'GATC_y
Cuts at: 1 383 1688 2327 5832 6299 6322 6657 6903
Size: 382 1305 639 3505 467 23 335 246
Cuts at: 6903 7915 8001 8013 8359 1
Size: 1012 86 12 346 449
Fragments arranged by size:
3505 1305 1012 639 467 449 382 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 224 1715 224
Size: 1491 7316
BtgI C'CryG_G
Cuts at: 107 189 1529 1586 1652 1970 2036 2067 2242
Size: 82 1340 57 66 318 66 31 175
Cuts at: 2242 2331 2376 2570 2936 3318 3773 4370 4479
Size: 89 45 194 366 382 455 597 109
Cuts at: 4479 4629 4709 4994 5139 5720 107
Size: 150 80 285 145 581 3194
Fragments arranged by size:
3194 1340 597 581 455 382 366 318
285 194 175 150 145 109 89 82
80 66 66 57 45 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1607 3900 4218 4326 6757 6938 7101 1607
Size: 2293 318 108 2431 181 163 3313
Fragments arranged by size:
3313 2431 2293 318 181 163 108
BtsI GCAGTG_nn'
Cuts at: 702 878 1322 1411 2273 2582 4719 5592 5604
Size: 176 444 89 862 309 2137 873 12
Cuts at: 5604 6050 702
Size: 446 3459
Fragments arranged by size:
3459 2137 873 862 446 444 309 176
89 12
ClaI AT'CG_AT
Cuts at: 4271 4313 4271
Size: 42 8765
DraI TTT'AAA
Cuts at: 5806 5806
Size: 8807
DraIII CAC_nnn'GTG
Cuts at: 1782 3351 1782
Size: 1569 7238
DrdI GACnn_nn'nnGTC
Cuts at: 240 3040 6513 7371 240
Size: 2800 3473 858 1676
Fragments arranged by size:
3473 2800 1676 858
EaeI y'GGCC_r
Cuts at: 651 948 1004 1214 2322 2573 3420 4409 4471
Size: 297 56 210 1108 251 847 989 62
Cuts at: 4471 4535 4621 4812 4911 5018 5338 5488 6392
Size: 64 86 191 99 107 320 150 904
Cuts at: 6392 6566 6957 6984 8329 651
Size: 174 391 27 1345 1129
Fragments arranged by size:
1345 1129 1108 989 904 847 391 320
297 251 210 191 174 150 107 99
86 64 62 56 27
EagI C'GGCC_G
Cuts at: 651 3420 6392 651
Size: 2769 2972 3066
Fragments arranged by size:
3066 2972 2769
EarI CTCTTCn'nnn_
Cuts at: 479 1064 6830 7040 479
Size: 585 5766 210 2246
Fragments arranged by size:
5766 2246 585 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 599 1917 2920 3602 4108 7015 7325 7471 8617
Size: 1318 1003 682 506 2907 310 146 1146
Cuts at: 8617 599
Size: 789
Fragments arranged by size:
2907 1318 1146 1003 789 682 506 310
146
EcoICRI GAG'CTC
Cuts at: 921 966 5001 5819 921
Size: 45 4035 818 3909
Fragments arranged by size:
4035 3909 818 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 982 1332 1843 2091 2149 2380 2578 3571
Size: 426 350 511 248 58 231 198 993
Cuts at: 3571 3649 3780 4006 4216 4404 4456 4563 4915
Size: 78 131 226 210 188 52 107 352
Cuts at: 4915 4958 5668 5788 6632 7064 7811 8079 556
Size: 43 710 120 844 432 747 268 1284
Fragments arranged by size:
1284 993 844 747 710 511 432 426
352 350 268 248 231 226 210 198
188 131 120 107 78 58 52 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2728 4504 2728
Size: 1776 7031
EcoO109I rG'GnC_Cy
Cuts at: 526 1188 1189 1818 1989 2115 2179 2807 2817
Size: 662 1 629 171 126 64 628 10
Cuts at: 2817 2889 3336 3877 3944 4353 4354 4705 5221
Size: 72 447 541 67 409 1 351 516
Cuts at: 5221 5946 526
Size: 725 3387
Fragments arranged by size:
3387 725 662 629 628 541 516 447
409 351 171 126 72 67 64 10
1 1
EcoRI G'AATT_C
Cuts at: 4185 5811 4185
Size: 1626 7181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1803 4011 6826 1803
Size: 2208 2815 3784
Fragments arranged by size:
3784 2815 2208
FseI GG_CCGG'CC
Cuts at: 2550 2550
Size: 8807
FspI TGC'GCA
Cuts at: 2212 4552 6588 2212
Size: 2340 2036 4431
Fragments arranged by size:
4431 2340 2036
FspAI rTGC'GCAy
Cuts at: 2212 4552 2212
Size: 2340 6467
HaeII r_GCGC'y
Cuts at: 188 339 1345 1388 3789 4110 4891 5057 5352
Size: 151 1006 43 2401 321 781 166 295
Cuts at: 5352 6241 6489 7511 8490 188
Size: 889 248 1022 979 505
Fragments arranged by size:
2401 1022 1006 979 889 781 505 321
295 248 166 151 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1436 1468 1660 1692 1783 1815 2372 2404 3238
Size: 32 192 32 91 32 557 32 834
Cuts at: 3238 3270 3936 3958 3968 3990 4135 4167 4429
Size: 32 666 22 10 22 145 32 262
Cuts at: 4429 4461 5437 5469 6649 6679 6681 6711 1436
Size: 32 976 32 1180 30 2 30 3532
Fragments arranged by size:
3532 1180 976 834 666 557 262 192
145 91 32 32 32 32 32 32
32 32 30 30 22 22 10 2
HincII GTy'rAC
Cuts at: 5379 5676 5846 5379
Size: 297 170 8340
Fragments arranged by size:
8340 297 170
HindIII A'AGCT_T
Cuts at: 5299 5299
Size: 8807
KasI G'GCGC_C
Cuts at: 184 1341 6237 6485 184
Size: 1157 4896 248 2506
Fragments arranged by size:
4896 2506 1157 248
KpnI G_GTAC'C
Cuts at: 3493 5827 3493
Size: 2334 6473
MluI A'CGCG_T
Cuts at: 8240 8240
Size: 8807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 731 1658 4499 4754 7478 7662 731
Size: 927 2841 255 2724 184 1876
Fragments arranged by size:
2841 2724 1876 927 255 184
MscI TGG'CCA
Cuts at: 1006 1216 2324 4473 4537 4623 4814 5020 5340
Size: 210 1108 2149 64 86 191 206 320
Cuts at: 5340 5490 6568 1006
Size: 150 1078 3245
Fragments arranged by size:
3245 2149 1108 1078 320 210 206 191
150 86 64
MslI CAynn'nnrTG
Cuts at: 304 520 1000 1399 1528 1585 2173 2278 2350
Size: 216 480 399 129 57 588 105 72
Cuts at: 2350 2557 2575 2938 3106 3484 4571 4714 4921
Size: 207 18 363 168 378 1087 143 207
Cuts at: 4921 5287 5597 5620 6796 6923 304
Size: 366 310 23 1176 127 2188
Fragments arranged by size:
2188 1176 1087 588 480 399 378 366
363 310 216 207 207 168 143 129
127 105 72 57 23 18
MspA1I CmG'CkG
Cuts at: 495 534 666 1177 1654 1851 2069 2111 2234
Size: 39 132 511 477 197 218 42 123
Cuts at: 2234 2244 2333 2435 2828 3173 3202 3393 3600
Size: 10 89 102 393 345 29 191 207
Cuts at: 3600 5748 5918 6232 6592 7605 7850 495
Size: 2148 170 314 360 1013 245 1452
Fragments arranged by size:
2148 1452 1013 511 477 393 360 345
314 245 218 207 197 191 170 132
123 102 89 42 39 29 10
NaeI GCC'GGC
Cuts at: 2548 6988 2548
Size: 4440 4367
NarI GG'CG_CC
Cuts at: 185 1342 6238 6486 185
Size: 1157 4896 248 2506
Fragments arranged by size:
4896 2506 1157 248
NcoI C'CATG_G
Cuts at: 107 189 3773 4370 4629 4709 4994 5720 107
Size: 82 3584 597 259 80 285 726 3194
Fragments arranged by size:
3584 3194 726 597 285 259 82 80
NdeI CA'TA_TG
Cuts at: 3636 3636
Size: 8807
NgoMIV G'CCGG_C
Cuts at: 2546 6986 2546
Size: 4440 4367
NheI G'CTAG_C
Cuts at: 2406 2406
Size: 8807
NspI r_CATG'y
Cuts at: 866 1380 2080 2287 2851 3519 4830 5259 5403
Size: 514 700 207 564 668 1311 429 144
Cuts at: 5403 5658 5682 5860 6360 7267 866
Size: 255 24 178 500 907 2406
Fragments arranged by size:
2406 1311 907 700 668 564 514 500
429 255 207 178 144 24
PasI CC'CwG_GG
Cuts at: 127 502 127
Size: 375 8432
PciI A'CATG_T
Cuts at: 5678 7263 5678
Size: 1585 7222
PflMI CCAn_nnn'nTGG
Cuts at: 4480 4630 4959 4480
Size: 150 329 8328
Fragments arranged by size:
8328 329 150
PfoI T'CCnGG_A
Cuts at: 959 3094 3662 5177 5645 959
Size: 2135 568 1515 468 4121
Fragments arranged by size:
4121 2135 1515 568 468
PmeI GTTT'AAAC
Cuts at: 5806 5806
Size: 8807
PmlI CAC'GTG
Cuts at: 4178 4818 4907 4178
Size: 640 89 8078
Fragments arranged by size:
8078 640 89
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4990 5022 7946 7978 4990
Size: 32 2924 32 5819
Fragments arranged by size:
5819 2924 32 32
PpuMI rG'GwC_Cy
Cuts at: 1818 1989 2115 2807 2817 2889 3877 3944 1818
Size: 171 126 692 10 72 988 67 6681
Fragments arranged by size:
6681 988 692 171 126 72 67 10
PspOMI G'GGCC_C
Cuts at: 1188 2178 4353 4705 1188
Size: 990 2175 352 5290
Fragments arranged by size:
5290 2175 990 352
PspXI vC'TCGA_Gb
Cuts at: 5002 5002
Size: 8807
PstI C_TGCA'G
Cuts at: 2648 2975 3284 3344 4726 5132 5599 5854 6539
Size: 327 309 60 1382 406 467 255 685
Cuts at: 6539 2648
Size: 4916
Fragments arranged by size:
4916 1382 685 467 406 327 309 255
60
PvuI CG_AT'CG
Cuts at: 104 3980 104
Size: 3876 4931
PvuII CAG'CTG
Cuts at: 495 534 666 1851 2111 3173 3202 5748 6232
Size: 39 132 1185 260 1062 29 2546 484
Cuts at: 6232 6592 495
Size: 360 2710
Fragments arranged by size:
2710 2546 1185 1062 484 360 260 132
39 29
RsrII CG'GwC_CG
Cuts at: 1974 7002 1974
Size: 5028 3779
SacI G_AGCT'C
Cuts at: 923 968 5003 5821 923
Size: 45 4035 818 3909
Fragments arranged by size:
4035 3909 818 45
SacII CC_GC'GG
Cuts at: 1655 2070 2245 2334 1655
Size: 415 175 89 8128
Fragments arranged by size:
8128 415 175 89
SalI G'TCGA_C
Cuts at: 5674 5844 5674
Size: 170 8637
SanDI GG'GwC_CC
Cuts at: 1989 2889 3877 1989
Size: 900 988 6919
Fragments arranged by size:
6919 988 900
SapI GCTCTTCn'nnn_
Cuts at: 1064 6830 7040 1064
Size: 5766 210 2831
Fragments arranged by size:
5766 2831 210
SbfI CC_TGCA'GG
Cuts at: 5854 5854
Size: 8807
ScaI AGT'ACT
Cuts at: 2032 2650 3298 4468 5335 5368 8324 2032
Size: 618 648 1170 867 33 2956 2515
Fragments arranged by size:
2956 2515 1170 867 648 618 33
SexAI A'CCwGG_T
Cuts at: 2117 2339 3347 2117
Size: 222 1008 7577
Fragments arranged by size:
7577 1008 222
SfcI C'TryA_G
Cuts at: 33 275 629 1227 1557 2644 2971 3280 3340
Size: 242 354 598 330 1087 327 309 60
Cuts at: 3340 4722 5128 5595 5850 6535 7528 7719 33
Size: 1382 406 467 255 685 993 191 1121
Fragments arranged by size:
1382 1121 1087 993 685 598 467 406
354 330 327 309 255 242 191 60
SfoI GGC'GCC
Cuts at: 186 1343 6239 6487 186
Size: 1157 4896 248 2506
Fragments arranged by size:
4896 2506 1157 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8807
SmaI CCC'GGG
Cuts at: 4284 4450 5443 5829 4284
Size: 166 993 386 7262
Fragments arranged by size:
7262 993 386 166
SmlI C'TyrA_G
Cuts at: 3013 3732 4129 4250 5002 5962 6877 7369 7631
Size: 719 397 121 752 960 915 492 262
Cuts at: 7631 3013
Size: 4189
Fragments arranged by size:
4189 960 915 752 719 492 397 262
121
SpeI A'CTAG_T
Cuts at: 67 460 67
Size: 393 8414
SphI G_CATG'C
Cuts at: 1380 3519 4830 5259 5658 5860 6360 1380
Size: 2139 1311 429 399 202 500 3827
Fragments arranged by size:
3827 2139 1311 500 429 399 202
StuI AGG'CCT
Cuts at: 4684 5203 4684
Size: 519 8288
StyI C'CwwG_G
Cuts at: 107 189 711 926 2486 2609 3526 3677 3773
Size: 82 522 215 1560 123 917 151 96
Cuts at: 3773 4370 4629 4709 4994 5027 5555 5720 5941
Size: 597 259 80 285 33 528 165 221
Cuts at: 5941 6295 107
Size: 354 2619
Fragments arranged by size:
2619 1560 917 597 528 522 354 285
259 221 215 165 151 123 96 82
80 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 436 865 897 2041 2145 2618 2622 2770 3858
Size: 429 32 1144 104 473 4 148 1088
Cuts at: 3858 4082 4086 6390 6523 6982 7191 436
Size: 224 4 2304 133 459 209 2052
Fragments arranged by size:
2304 2052 1144 1088 473 459 429 224
209 148 133 104 32 4 4
TatI w'GTAC_w
Cuts at: 427 1745 2030 2648 3221 3296 4466 5333 5366
Size: 1318 285 618 573 75 1170 867 33
Cuts at: 5366 5393 8322 427
Size: 27 2929 912
Fragments arranged by size:
2929 1318 1170 912 867 618 573 285
75 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3337 3369 3850 3882 3940 3972 8324 8356 3337
Size: 32 481 32 58 32 4352 32 3788
Fragments arranged by size:
4352 3788 481 58 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2865 6604 2865
Size: 3739 5068
XbaI T'CTAG_A
Cuts at: 3567 5838 3567
Size: 2271 6536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1014 1395 2115 3501 4086 5142 6302 1014
Size: 381 720 1386 585 1056 1160 3519
Fragments arranged by size:
3519 1386 1160 1056 720 585 381
XhoI C'TCGA_G
Cuts at: 4250 5002 4250
Size: 752 8055
XmaI C'CCGG_G
Cuts at: 4282 4448 5441 5827 4282
Size: 166 993 386 7262
Fragments arranged by size:
7262 993 386 166
ZraI GAC'GTC
Cuts at: 2400 3158 2400
Size: 758 8049
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BaeI BcgI BfrBI BsmBI
BssHII BstZ17I CspCI EcoRV HpaI MfeI NotI NruI
NsiI PacI PshAI PsiI PsrI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI