Restriction map for
pF1KB1755
(Circular) (Six-base) MAPSORT of: pF1KB1755.seq Check: 1556 from: 1 to: 7508
pF1KB1755 7508 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1148 1148
Size: 7508
AatII G_ACGT'C
Cuts at: 292 941 1987 2210 292
Size: 649 1046 223 5590
Fragments arranged by size:
5590 1046 649 223
AccI GT'mk_AC
Cuts at: 4546 4546
Size: 7508
Acc65I G'GTAC_C
Cuts at: 185 371 1055 4524 185
Size: 186 684 3469 3169
Fragments arranged by size:
3469 3169 684 186
AclI AA'CG_TT
Cuts at: 7348 7348
Size: 7508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 954 1048 1116 2643 2745 3838 3841 5333 5765
Size: 94 68 1527 102 1093 3 1492 432
Cuts at: 5765 6512 6780 954
Size: 747 268 1682
Fragments arranged by size:
1682 1527 1492 1093 747 432 268 102
94 68 3
AfeI AGC'GCT
Cuts at: 415 415
Size: 7508
AflIII A'CryG_T
Cuts at: 2585 2807 2918 4860 5964 6941 2585
Size: 222 111 1942 1104 977 3152
Fragments arranged by size:
3152 1942 1104 977 222 111
AgeI A'CCGG_T
Cuts at: 1413 1651 5896 1413
Size: 238 4245 3025
Fragments arranged by size:
4245 3025 238
AhdI GACnn_n'nnGTC
Cuts at: 3075 3075
Size: 7508
AleI CACnn'nnGTG
Cuts at: 1654 1654
Size: 7508
AlwNI CAG_nnn'CTG
Cuts at: 317 381 4813 6380 317
Size: 64 4432 1567 1445
Fragments arranged by size:
4432 1567 1445 64
ApaI G_GGCC'C
Cuts at: 1254 1254
Size: 7508
ApaLI G'TGCA_C
Cuts at: 1002 1259 1657 2119 6278 1002
Size: 257 398 462 4159 2232
Fragments arranged by size:
4159 2232 462 398 257
ApoI r'AATT_y
Cuts at: 2190 2311 3475 4093 4512 6861 7117 2190
Size: 121 1164 618 419 2349 256 2581
Fragments arranged by size:
2581 2349 1164 618 419 256 121
AseI AT'TA_AT
Cuts at: 20 3260 20
Size: 3240 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7508
AvaI C'yCGr_G
Cuts at: 1184 1809 3109 4344 4528 1184
Size: 625 1300 1235 184 4164
Fragments arranged by size:
4164 1300 1235 625 184
BamHI G'GATC_C
Cuts at: 4533 4533
Size: 7508
BanI G'GyrC_C
Cuts at: 185 371 1055 2842 4524 4938 5186 5221 185
Size: 186 684 1787 1682 414 248 35 2472
Fragments arranged by size:
2472 1787 1682 684 414 248 186 35
BanII G_rGCy'C
Cuts at: 567 1254 1345 2081 2495 2546 3110 3662 4522
Size: 687 91 736 414 51 564 552 860
Cuts at: 4522 5552 567
Size: 1030 2523
Fragments arranged by size:
2523 1030 860 736 687 564 552 414
91 51
BbeI G_GCGC'C
Cuts at: 4942 5190 4942
Size: 248 7260
BbsI GAAGACnn'nnnn_
Cuts at: 257 2751 3401 4192 257
Size: 2494 650 791 3573
Fragments arranged by size:
3573 2494 791 650
BbvCI CC'TCA_GC
Cuts at: 421 1693 4488 421
Size: 1272 2795 3441
Fragments arranged by size:
3441 2795 1272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 882 916 3536 3570 4128 4162 882
Size: 34 2620 34 558 34 4228
Fragments arranged by size:
4228 2620 558 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2095 5406 6167 7508 2095
Size: 3311 761 1341 2095
Fragments arranged by size:
3311 2095 1341 761
BclI T'GATC_A
Cuts at: 5028 5028
Size: 7508
BfrBI ATG'CAT
Cuts at: 3433 3433
Size: 7508
BglI GCCn_nnn'nGGC
Cuts at: 4492 4492
Size: 7508
BglII A'GATC_T
Cuts at: 3917 5023 7060 3917
Size: 1106 2037 4365
Fragments arranged by size:
4365 2037 1106
BlpI GC'TnA_GC
Cuts at: 3221 4267 4620 3221
Size: 1046 353 6109
Fragments arranged by size:
6109 1046 353
Bme1580I G_kGCm'C
Cuts at: 1006 1254 1263 1661 2123 5133 5226 6282 1006
Size: 248 9 398 462 3010 93 1056 2232
Fragments arranged by size:
3010 2232 1056 462 398 248 93 9
BmgBI CAC'GTC
Cuts at: 1068 2588 2810 4164 4398 1068
Size: 1520 222 1354 234 4178
Fragments arranged by size:
4178 1520 1354 234 222
BmrI ACTGGGnnnn_n'
Cuts at: 2498 3528 4720 4801 5136 7280 2498
Size: 1030 1192 81 335 2144 2726
Fragments arranged by size:
2726 2144 1192 1030 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4296 5875 4296
Size: 1579 5929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 769 1497 2696 3066 3199 3661 264
Size: 505 728 1199 370 133 462 4111
Fragments arranged by size:
4111 1199 728 505 462 370 133
Bpu10I CC'TnA_GC
Cuts at: 421 1693 1885 2012 4488 421
Size: 1272 192 127 2476 3441
Fragments arranged by size:
3441 2476 1272 192 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 982 3583 4684 5563 6055 6353 270
Size: 712 2601 1101 879 492 298 1425
Fragments arranged by size:
2601 1425 1101 879 712 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1524 2733 2829 7212 1524
Size: 1209 96 4383 1820
Fragments arranged by size:
4383 1820 1209 96
BsaAI yAC'GTr
Cuts at: 2043 4861 2043
Size: 2818 4690
BsaBI GATnn'nnATC
Cuts at: 6 5022 7059 6
Size: 5016 2037 455
Fragments arranged by size:
5016 2037 455
BsaHI Gr'CG_yC
Cuts at: 289 938 1018 1193 1286 1628 1984 2207 4939
Size: 649 80 175 93 342 356 223 2732
Cuts at: 4939 5187 5889 7385 289
Size: 248 702 1496 412
Fragments arranged by size:
2732 1496 702 649 412 356 342 248
223 175 93 80
BsaWI w'CCGG_w
Cuts at: 1413 1651 3021 4063 4697 5218 5896 6170 6317
Size: 238 1370 1042 634 521 678 274 147
Cuts at: 6317 6728 1413
Size: 411 2193
Fragments arranged by size:
2193 1370 1042 678 634 521 411 274
238 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 598 628 1891 1921 2266 2296 598
Size: 30 1263 30 345 30 5810
Fragments arranged by size:
5810 1263 345 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 457 2035 2254 2680 2965 3652 364
Size: 93 1578 219 426 285 687 4220
Fragments arranged by size:
4220 1578 687 426 285 219 93
BseYI C'CCAG_C
Cuts at: 1697 2031 2398 4451 4933 5691 6268 6764 1697
Size: 334 367 2053 482 758 577 496 2441
Fragments arranged by size:
2441 2053 758 577 496 482 367 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1057 1126 1417 1520 2017 2239 3397 3816
Size: 375 69 291 103 497 222 1158 419
Cuts at: 3816 4188 682
Size: 372 4002
Fragments arranged by size:
4002 1158 497 419 375 372 291 222
103 69
BsiEI CG_ry'CG
Cuts at: 104 262 5050 5096 6304 104
Size: 158 4788 46 1208 1308
Fragments arranged by size:
4788 1308 1208 158 46
BsiHKAI G_wGCw'C
Cuts at: 337 1006 1263 1661 2123 2513 3110 3227 3662
Size: 669 257 398 462 390 597 117 435
Cuts at: 3662 4277 4522 5300 5490 6282 337
Size: 615 245 778 190 792 1563
Fragments arranged by size:
1563 792 778 669 615 597 462 435
398 390 257 245 190 117
BsmI GAATG_Cn'
Cuts at: 3433 3433
Size: 7508
BsmBI CGTCTCn'nnnn_
Cuts at: 854 979 2927 854
Size: 125 1948 5435
Fragments arranged by size:
5435 1948 125
Bsp1286I G_dGCh'C
Cuts at: 337 567 1006 1254 1263 1345 1661 2081 2123
Size: 230 439 248 9 82 316 420 42
Cuts at: 2123 2495 2513 2546 3110 3227 3662 4277 4522
Size: 372 18 33 564 117 435 615 245
Cuts at: 4522 5133 5226 5300 5490 5552 6282 337
Size: 611 93 74 190 62 730 1563
Fragments arranged by size:
1563 730 615 611 564 439 435 420
372 316 248 245 230 190 117 93
82 74 62 42 33 18 9
BspEI T'CCGG_A
Cuts at: 3021 4063 6728 3021
Size: 1042 2665 3801
Fragments arranged by size:
3801 2665 1042
BspHI T'CATG_A
Cuts at: 3384 3863 6684 3384
Size: 479 2821 4208
Fragments arranged by size:
4208 2821 479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 197 359 1148 2036 2744 4130 4210 4558 4772
Size: 162 789 888 708 1386 80 348 214
Cuts at: 4772 5074 5455 197
Size: 302 381 2250
Fragments arranged by size:
2250 1386 888 789 708 381 348 302
214 162 80
BsrBI CCG'CTC
Cuts at: 50 552 1731 1766 5800 5854 7338 7503 50
Size: 502 1179 35 4034 54 1484 165 55
Fragments arranged by size:
4034 1484 1179 502 165 55 54 35
BsrDI GCAATG_nn'
Cuts at: 1410 3138 3791 5420 1410
Size: 1728 653 1629 3498
Fragments arranged by size:
3498 1728 1629 653
BsrFI r'CCGG_y
Cuts at: 1413 1651 1874 2479 5506 5687 5896 1413
Size: 238 223 605 3027 181 209 3025
Fragments arranged by size:
3027 3025 605 238 223 209 181
BsrGI T'GTAC_A
Cuts at: 1163 4412 1163
Size: 3249 4259
BssHII G'CGCG_C
Cuts at: 1044 1044
Size: 7508
BssSI C'ACGA_G
Cuts at: 2380 5779 6137 2380
Size: 3399 358 3751
Fragments arranged by size:
3751 3399 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2779 4885 2779
Size: 2106 5402
BstBI TT'CG_AA
Cuts at: 5869 5869
Size: 7508
BstEII G'GTnAC_C
Cuts at: 963 4167 963
Size: 3204 4304
BstXI CCAn_nnnn'nTGG
Cuts at: 657 2251 657
Size: 1594 5914
BstYI r'GATC_y
Cuts at: 1 572 1804 2499 3917 4430 4533 5000 5023
Size: 571 1232 695 1418 513 103 467 23
Cuts at: 5023 5358 5604 6616 6702 6714 7060 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1418 1232 1012 695 571 513 467 449
346 335 246 103 86 23 12
BtgI C'CryG_G
Cuts at: 656 810 1265 1716 1754 2132 2987 3690 656
Size: 154 455 451 38 378 855 703 4474
Fragments arranged by size:
4474 855 703 455 451 378 154 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1076 5458 5639 5802 1076
Size: 4382 181 163 2782
Fragments arranged by size:
4382 2782 181 163
BtsI GCAGTG_nn'
Cuts at: 670 776 800 1282 1405 1490 2005 3628 4751
Size: 106 24 482 123 85 515 1623 1123
Cuts at: 4751 670
Size: 3427
Fragments arranged by size:
3427 1623 1123 515 482 123 106 85
24
ClaI AT'CG_AT
Cuts at: 913 3342 913
Size: 2429 5079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2771 2806 2771
Size: 35 7473
DraI TTT'AAA
Cuts at: 2723 2976 4507 2723
Size: 253 1531 5724
Fragments arranged by size:
5724 1531 253
DraIII CAC_nnn'GTG
Cuts at: 2936 2936
Size: 7508
DrdI GACnn_nn'nnGTC
Cuts at: 1240 2919 3271 5214 6072 1240
Size: 1679 352 1943 858 2676
Fragments arranged by size:
2676 1943 1679 858 352
EaeI y'GGCC_r
Cuts at: 386 797 2057 2277 2477 5093 5267 5658 5685
Size: 411 1260 220 200 2616 174 391 27
Cuts at: 5685 7030 386
Size: 1345 864
Fragments arranged by size:
2616 1345 1260 864 411 391 220 200
174 27
EagI C'GGCC_G
Cuts at: 5093 5093
Size: 7508
EarI CTCTTCn'nnn_
Cuts at: 133 542 3098 3446 3950 5531 5741 133
Size: 409 2556 348 504 1581 210 1900
Fragments arranged by size:
2556 1900 1581 504 409 348 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5716 6026 6172 7318 5716
Size: 310 146 1146 5906
Fragments arranged by size:
5906 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3108 3660 4520 3108
Size: 552 860 6096
Fragments arranged by size:
6096 860 552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 769 954 1048 1116 1497 2643 2696 2745
Size: 505 185 94 68 381 1146 53 49
Cuts at: 2745 3066 3199 3661 3838 3841 5333 5765 6512
Size: 321 133 462 177 3 1492 432 747
Cuts at: 6512 6780 264
Size: 268 992
Fragments arranged by size:
1492 1146 992 747 505 462 432 381
321 268 185 177 133 94 68 53
49 3
EcoNI CCTnn'n_nnAGG
Cuts at: 972 1144 972
Size: 172 7336
EcoO109I rG'GnC_Cy
Cuts at: 1158 1250 1251 1642 1725 2025 2304 2610 3365
Size: 92 1 391 83 300 279 306 755
Cuts at: 3365 3493 4647 1158
Size: 128 1154 4019
Fragments arranged by size:
4019 1154 755 391 306 300 279 128
92 83 1
EcoRI G'AATT_C
Cuts at: 4093 4512 4093
Size: 419 7089
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1119 3027 3425 3565 3726 5527 1119
Size: 1908 398 140 161 1801 3100
Fragments arranged by size:
3100 1908 1801 398 161 140
FspI TGC'GCA
Cuts at: 1174 5289 1174
Size: 4115 3393
HaeII r_GCGC'y
Cuts at: 417 1211 4942 5190 6212 7191 417
Size: 794 3731 248 1022 979 734
Fragments arranged by size:
3731 1022 979 794 734 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 290 322 598 630 978 1010 1891 1923 2266
Size: 32 276 32 348 32 881 32 343
Cuts at: 2266 2298 2669 2701 3061 3093 3214 3246 4179
Size: 32 371 32 360 32 121 32 933
Cuts at: 4179 4211 5350 5380 5382 5412 290
Size: 32 1139 30 2 30 2386
Fragments arranged by size:
2386 1139 933 881 371 360 348 343
276 121 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1785 2472 4547 1785
Size: 687 2075 4746
Fragments arranged by size:
4746 2075 687
HindIII A'AGCT_T
Cuts at: 4070 4070
Size: 7508
KasI G'GCGC_C
Cuts at: 4938 5186 4938
Size: 248 7260
KpnI G_GTAC'C
Cuts at: 189 375 1059 4528 189
Size: 186 684 3469 3169
Fragments arranged by size:
3469 3169 684 186
MluI A'CGCG_T
Cuts at: 2918 6941 2918
Size: 4023 3485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2792 6179 6363 2792
Size: 3387 184 3937
Fragments arranged by size:
3937 3387 184
MscI TGG'CCA
Cuts at: 799 5269 799
Size: 4470 3038
MslI CAynn'nnrTG
Cuts at: 655 1012 1654 2215 2249 3521 3908 5497 5624
Size: 357 642 561 34 1272 387 1589 127
Cuts at: 5624 655
Size: 2539
Fragments arranged by size:
2539 1589 1272 642 561 387 357 127
34
MspA1I CmG'CkG
Cuts at: 258 1239 1697 1772 1798 4492 4619 4933 5293
Size: 981 458 75 26 2694 127 314 360
Cuts at: 5293 6306 6551 258
Size: 1013 245 1215
Fragments arranged by size:
2694 1215 1013 981 458 360 314 245
127 75 26
NaeI GCC'GGC
Cuts at: 2481 5689 2481
Size: 3208 4300
NarI GG'CG_CC
Cuts at: 4939 5187 4939
Size: 248 7260
NcoI C'CATG_G
Cuts at: 1265 1265
Size: 7508
NdeI CA'TA_TG
Cuts at: 3935 3935
Size: 7508
NgoMIV G'CCGG_C
Cuts at: 2479 5687 2479
Size: 3208 4300
NsiI A_TGCA'T
Cuts at: 3435 3435
Size: 7508
NspI r_CATG'y
Cuts at: 4561 5061 5968 4561
Size: 500 907 6101
Fragments arranged by size:
6101 907 500
PasI CC'CwG_GG
Cuts at: 294 294
Size: 7508
PciI A'CATG_T
Cuts at: 5964 5964
Size: 7508
PflMI CCAn_nnn'nTGG
Cuts at: 381 948 2072 381
Size: 567 1124 5817
Fragments arranged by size:
5817 1124 567
PfoI T'CCnGG_A
Cuts at: 4433 4433
Size: 7508
PmeI GTTT'AAAC
Cuts at: 4507 4507
Size: 7508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6647 6679 6647
Size: 32 7476
PpuMI rG'GwC_Cy
Cuts at: 1725 2025 2304 3365 1725
Size: 300 279 1061 5868
Fragments arranged by size:
5868 1061 300 279
PshAI GACnn'nnGTC
Cuts at: 819 1936 2755 819
Size: 1117 819 5572
Fragments arranged by size:
5572 1117 819
PspOMI G'GGCC_C
Cuts at: 1250 1250
Size: 7508
PspXI vC'TCGA_Gb
Cuts at: 3109 3109
Size: 7508
PstI C_TGCA'G
Cuts at: 313 2741 3974 4555 5240 313
Size: 2428 1233 581 685 2581
Fragments arranged by size:
2581 2428 1233 685 581
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7508
PvuII CAG'CTG
Cuts at: 1697 1772 1798 4933 5293 1697
Size: 75 26 3135 360 3912
Fragments arranged by size:
3912 3135 360 75 26
RsrII CG'GwC_CG
Cuts at: 5703 5703
Size: 7508
SacI G_AGCT'C
Cuts at: 3110 3662 4522 3110
Size: 552 860 6096
Fragments arranged by size:
6096 860 552
SalI G'TCGA_C
Cuts at: 4545 4545
Size: 7508
SapI GCTCTTCn'nnn_
Cuts at: 542 3098 5531 5741 542
Size: 2556 2433 210 2309
Fragments arranged by size:
2556 2433 2309 210
SbfI CC_TGCA'GG
Cuts at: 4555 4555
Size: 7508
ScaI AGT'ACT
Cuts at: 3511 7025 3511
Size: 3514 3994
SexAI A'CCwGG_T
Cuts at: 2932 2932
Size: 7508
SfcI C'TryA_G
Cuts at: 33 309 2737 3970 4551 5236 6229 6420 33
Size: 276 2428 1233 581 685 993 191 1121
Fragments arranged by size:
2428 1233 1121 993 685 581 276 191
SfoI GGC'GCC
Cuts at: 4940 5188 4940
Size: 248 7260
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7508
SgrAI Cr'CCGG_yG
Cuts at: 1413 1651 1413
Size: 238 7270
SmaI CCC'GGG
Cuts at: 4530 4530
Size: 7508
SmlI C'TyrA_G
Cuts at: 249 997 3109 3598 4663 5578 6070 6332 249
Size: 748 2112 489 1065 915 492 262 1425
Fragments arranged by size:
2112 1425 1065 915 748 492 489 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7508
SphI G_CATG'C
Cuts at: 4561 5061 4561
Size: 500 7008
SspI AAT'ATT
Cuts at: 174 174
Size: 7508
StuI AGG'CCT
Cuts at: 1120 2527 1120
Size: 1407 6101
StyI C'CwwG_G
Cuts at: 1265 1547 2591 2846 4642 4996 1265
Size: 282 1044 255 1796 354 3777
Fragments arranged by size:
3777 1796 1044 354 282 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 241 1278 1382 1383 1722 2021 2412 5091 5224
Size: 1037 104 1 339 299 391 2679 133
Cuts at: 5224 5683 5892 241
Size: 459 209 1857
Fragments arranged by size:
2679 1857 1037 459 391 339 299 209
133 104 1
TatI w'GTAC_w
Cuts at: 713 1163 1736 2144 2618 3008 3509 4352 4412
Size: 450 573 408 474 390 501 843 60
Cuts at: 4412 7023 713
Size: 2611 1198
Fragments arranged by size:
2611 1198 843 573 501 474 450 408
390 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1261 1293 2219 2251 2636 2668 7025 7057 1261
Size: 32 926 32 385 32 4357 32 1712
Fragments arranged by size:
4357 1712 926 385 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1300 5305 1300
Size: 4005 3503
XbaI T'CTAG_A
Cuts at: 4539 4539
Size: 7508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 834 1986 3090 5003 834
Size: 1152 1104 1913 3339
Fragments arranged by size:
3339 1913 1152 1104
XhoI C'TCGA_G
Cuts at: 3109 3109
Size: 7508
XmaI C'CCGG_G
Cuts at: 4528 4528
Size: 7508
XmnI GAAnn'nnTTC
Cuts at: 1099 1099
Size: 7508
ZraI GAC'GTC
Cuts at: 290 939 1985 2208 290
Size: 649 1046 223 5590
Fragments arranged by size:
5590 1046 649 223
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AlfI AloI AscI AvrII BaeI BmtI BsiWI
BstZ17I Bsu36I EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PmlI PsiI PsrI SacII SanDI
SfiI SnaBI SrfI SwaI