Restriction map for
pF1KB1986
(Circular) (Six-base) MAPSORT of: pF1KB1986.seq Check: 3767 from: 1 to: 6422
pF1KB1986 6422 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1352 3460 1352
Size: 2108 4314
Acc65I G'GTAC_C
Cuts at: 3438 3438
Size: 6422
AclI AA'CG_TT
Cuts at: 2327 6262 2327
Size: 3935 2487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 2051 2620 2834 4247 4679 5426 5694 1396
Size: 655 569 214 1413 432 747 268 2124
Fragments arranged by size:
2124 1413 747 655 569 432 268 214
AflII C'TTAA_G
Cuts at: 2428 2428
Size: 6422
AflIII A'CryG_T
Cuts at: 2826 3774 4878 5855 2826
Size: 948 1104 977 3393
Fragments arranged by size:
3393 1104 977 948
AgeI A'CCGG_T
Cuts at: 4810 4810
Size: 6422
AleI CACnn'nnGTG
Cuts at: 1508 1508
Size: 6422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2783 2783
Size: 6422
AlwNI CAG_nnn'CTG
Cuts at: 2277 3727 5294 2277
Size: 1450 1567 3405
Fragments arranged by size:
3405 1567 1450
ApaLI G'TGCA_C
Cuts at: 5192 5192
Size: 6422
ApoI r'AATT_y
Cuts at: 405 1183 1648 1769 2157 2249 2806 2858 2923
Size: 778 465 121 388 92 557 52 65
Cuts at: 2923 3426 5775 6031 405
Size: 503 2349 256 796
Fragments arranged by size:
2349 796 778 557 503 465 388 256
121 92 65 52
AseI AT'TA_AT
Cuts at: 20 20
Size: 6422
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6422
AvaI C'yCGr_G
Cuts at: 118 2990 3442 118
Size: 2872 452 3098
Fragments arranged by size:
3098 2872 452
BamHI G'GATC_C
Cuts at: 1518 2256 3447 1518
Size: 738 1191 4493
Fragments arranged by size:
4493 1191 738
BanI G'GyrC_C
Cuts at: 3438 3852 4100 4135 3438
Size: 414 248 35 5725
Fragments arranged by size:
5725 414 248 35
BanII G_rGCy'C
Cuts at: 1854 2485 3436 4466 1854
Size: 631 951 1030 3810
Fragments arranged by size:
3810 1030 951 631
BbeI G_GCGC'C
Cuts at: 3856 4104 3856
Size: 248 6174
BbsI GAAGACnn'nnnn_
Cuts at: 605 2120 2218 2321 2354 3187 605
Size: 1515 98 103 33 833 3840
Fragments arranged by size:
3840 1515 833 103 98 33
BciVI GTATCCnnnnn_n'
Cuts at: 1889 2527 4320 5081 6422 1889
Size: 638 1793 761 1341 1889
Fragments arranged by size:
1889 1793 1341 761 638
BclI T'GATC_A
Cuts at: 3942 3942
Size: 6422
BglI GCCn_nnn'nGGC
Cuts at: 2149 2149
Size: 6422
BglII A'GATC_T
Cuts at: 1218 2133 3937 5974 1218
Size: 915 1804 2037 1666
Fragments arranged by size:
2037 1804 1666 915
BlpI GC'TnA_GC
Cuts at: 377 3534 377
Size: 3157 3265
Bme1580I G_kGCm'C
Cuts at: 4047 4140 5196 4047
Size: 93 1056 5273
Fragments arranged by size:
5273 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2225 2676 3634 3715 4050 6194 2225
Size: 451 958 81 335 2144 2453
Fragments arranged by size:
2453 2144 958 451 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4789 4789
Size: 6422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2356 2356
Size: 6422
Bpu10I CC'TnA_GC
Cuts at: 2396 2396
Size: 6422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 1655 1740 2655 2848 3598 4477 4969 5267
Size: 1323 85 915 193 750 879 492 298
Cuts at: 5267 332
Size: 1487
Fragments arranged by size:
1487 1323 915 879 750 492 298 193
85
BsaI GGTCTCn'nnnn_
Cuts at: 3332 6126 3332
Size: 2794 3628
BsaAI yAC'GTr
Cuts at: 2829 3775 2829
Size: 946 5476
BsaBI GATnn'nnATC
Cuts at: 6 3936 5973 6
Size: 3930 2037 455
Fragments arranged by size:
3930 2037 455
BsaHI Gr'CG_yC
Cuts at: 130 3853 4101 4803 6299 130
Size: 3723 248 702 1496 253
Fragments arranged by size:
3723 1496 702 253 248
BsaWI w'CCGG_w
Cuts at: 708 3611 4132 4810 5084 5231 5642 708
Size: 2903 521 678 274 147 411 1488
Fragments arranged by size:
2903 1488 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1844 1853 1862 1928 1958 1973 1844
Size: 9 9 66 30 15 6293
Fragments arranged by size:
6293 66 30 15 9 9
BseYI C'CCAG_C
Cuts at: 716 3847 4605 5182 5678 716
Size: 3131 758 577 496 1460
Fragments arranged by size:
3131 1460 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3347 3347
Size: 6422
BsiEI CG_ry'CG
Cuts at: 104 643 3964 4010 5218 104
Size: 539 3321 46 1208 1308
Fragments arranged by size:
3321 1308 1208 539 46
BsiHKAI G_wGCw'C
Cuts at: 1854 2485 3436 4214 4404 5196 1854
Size: 631 951 778 190 792 3080
Fragments arranged by size:
3080 951 792 778 631 190
BsmI GAATG_Cn'
Cuts at: 607 3331 607
Size: 2724 3698
BsmBI CGTCTCn'nnnn_
Cuts at: 1315 2512 1315
Size: 1197 5225
Bsp1286I G_dGCh'C
Cuts at: 1854 2485 3436 4047 4140 4214 4404 4466 5196
Size: 631 951 611 93 74 190 62 730
Cuts at: 5196 1854
Size: 3080
Fragments arranged by size:
3080 951 730 631 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 5642 5642
Size: 6422
BspHI T'CATG_A
Cuts at: 630 5598 630
Size: 4968 1454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3472 3686 3988 4369 3472
Size: 214 302 381 5525
Fragments arranged by size:
5525 381 302 214
BsrBI CCG'CTC
Cuts at: 50 139 4714 4768 6252 6417 50
Size: 89 4575 54 1484 165 55
Fragments arranged by size:
4575 1484 165 89 55 54
BsrDI GCAATG_nn'
Cuts at: 1795 4334 1795
Size: 2539 3883
BsrFI r'CCGG_y
Cuts at: 4420 4601 4810 4420
Size: 181 209 6032
Fragments arranged by size:
6032 209 181
BssSI C'ACGA_G
Cuts at: 4693 5051 4693
Size: 358 6064
BstAPI GCAn_nnn'nTGC
Cuts at: 3199 3799 3199
Size: 600 5822
BstBI TT'CG_AA
Cuts at: 1164 1734 4783 1164
Size: 570 3049 2803
Fragments arranged by size:
3049 2803 570
BstXI CCAn_nnnn'nTGG
Cuts at: 2289 2289
Size: 6422
BstYI r'GATC_y
Cuts at: 1 429 1218 1518 2133 2256 3447 3914 3937
Size: 428 789 300 615 123 1191 467 23
Cuts at: 3937 4272 4518 5530 5616 5628 5974 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1191 1012 789 615 467 449 428 346
335 300 246 123 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 685 685
Size: 6422
BtgI C'CryG_G
Cuts at: 107 107
Size: 6422
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 370 4372 4553 4716 370
Size: 4002 181 163 2076
Fragments arranged by size:
4002 2076 181 163
BtsI GCAGTG_nn'
Cuts at: 542 1382 1503 1515 3149 3665 542
Size: 840 121 12 1634 516 3299
Fragments arranged by size:
3299 1634 840 516 121 12
ClaI AT'CG_AT
Cuts at: 1553 1553
Size: 6422
DraI TTT'AAA
Cuts at: 3421 3421
Size: 6422
DrdI GACnn_nn'nnGTC
Cuts at: 4128 4986 4128
Size: 858 5564
EaeI y'GGCC_r
Cuts at: 732 4007 4181 4572 4599 5944 732
Size: 3275 174 391 27 1345 1210
Fragments arranged by size:
3275 1345 1210 391 174 27
EagI C'GGCC_G
Cuts at: 4007 4007
Size: 6422
EarI CTCTTCn'nnn_
Cuts at: 206 464 983 1862 2038 3089 3159 3170 4445
Size: 258 519 879 176 1051 70 11 1275
Cuts at: 4445 4655 206
Size: 210 1973
Fragments arranged by size:
1973 1275 1051 879 519 258 210 176
70 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4630 4940 5086 6232 4630
Size: 310 146 1146 4820
Fragments arranged by size:
4820 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1852 2483 3434 1852
Size: 631 951 4840
Fragments arranged by size:
4840 951 631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 2051 2356 2620 2834 4247 4679 5426 5694
Size: 655 305 264 214 1413 432 747 268
Cuts at: 5694 1396
Size: 2124
Fragments arranged by size:
2124 1413 747 655 432 305 268 264
214
EcoNI CCTnn'n_nnAGG
Cuts at: 1973 1973
Size: 6422
EcoO109I rG'GnC_Cy
Cuts at: 1777 1933 3561 1777
Size: 156 1628 4638
Fragments arranged by size:
4638 1628 156
EcoRI G'AATT_C
Cuts at: 1183 1648 1769 3426 1183
Size: 465 121 1657 4179
Fragments arranged by size:
4179 1657 465 121
EcoRV GAT'ATC
Cuts at: 2799 2799
Size: 6422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1696 2721 4441 1696
Size: 1025 1720 3677
Fragments arranged by size:
3677 1720 1025
FspI TGC'GCA
Cuts at: 4203 4203
Size: 6422
HaeII r_GCGC'y
Cuts at: 3856 4104 5126 6105 3856
Size: 248 1022 979 4173
Fragments arranged by size:
4173 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 382 414 1535 1567 2684 2716 2748 3285 3317
Size: 32 1121 32 1117 32 32 537 32
Cuts at: 3317 4264 4294 4296 4326 382
Size: 947 30 2 30 2478
Fragments arranged by size:
2478 1121 1117 947 537 32 32 32
32 32 30 30 2
HincII GTy'rAC
Cuts at: 723 3461 723
Size: 2738 3684
HindIII A'AGCT_T
Cuts at: 772 2613 772
Size: 1841 4581
HpaI GTT'AAC
Cuts at: 723 723
Size: 6422
KasI G'GCGC_C
Cuts at: 3852 4100 3852
Size: 248 6174
KpnI G_GTAC'C
Cuts at: 3442 3442
Size: 6422
MfeI C'AATT_G
Cuts at: 917 1064 2052 917
Size: 147 988 5287
Fragments arranged by size:
5287 988 147
MluI A'CGCG_T
Cuts at: 5855 5855
Size: 6422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 540 963 2628 5093 5277 540
Size: 423 1665 2465 184 1685
Fragments arranged by size:
2465 1685 1665 423 184
MscI TGG'CCA
Cuts at: 734 4183 734
Size: 3449 2973
MslI CAynn'nnrTG
Cuts at: 1508 3383 4411 4538 1508
Size: 1875 1028 127 3392
Fragments arranged by size:
3392 1875 1028 127
MspA1I CmG'CkG
Cuts at: 716 2737 3533 3847 4207 5220 5465 716
Size: 2021 796 314 360 1013 245 1673
Fragments arranged by size:
2021 1673 1013 796 360 314 245
NaeI GCC'GGC
Cuts at: 4603 4603
Size: 6422
NarI GG'CG_CC
Cuts at: 3853 4101 3853
Size: 248 6174
NcoI C'CATG_G
Cuts at: 107 107
Size: 6422
NdeI CA'TA_TG
Cuts at: 2310 2310
Size: 6422
NgoMIV G'CCGG_C
Cuts at: 4601 4601
Size: 6422
NruI TCG'CGA
Cuts at: 355 355
Size: 6422
NspI r_CATG'y
Cuts at: 1119 2307 2355 3475 3975 4882 1119
Size: 1188 48 1120 500 907 2659
Fragments arranged by size:
2659 1188 1120 907 500 48
PciI A'CATG_T
Cuts at: 4878 4878
Size: 6422
PflMI CCAn_nnn'nTGG
Cuts at: 1954 2688 1954
Size: 734 5688
PmeI GTTT'AAAC
Cuts at: 3421 3421
Size: 6422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2685 5561 5593 2653
Size: 32 2876 32 3482
Fragments arranged by size:
3482 2876 32 32
PpuMI rG'GwC_Cy
Cuts at: 1777 1933 1777
Size: 156 6266
PsiI TTA'TAA
Cuts at: 1126 1466 1126
Size: 340 6082
PstI C_TGCA'G
Cuts at: 375 1510 1623 1788 3469 4154 375
Size: 1135 113 165 1681 685 2643
Fragments arranged by size:
2643 1681 1135 685 165 113
PvuI CG_AT'CG
Cuts at: 104 643 104
Size: 539 5883
PvuII CAG'CTG
Cuts at: 716 3847 4207 716
Size: 3131 360 2931
Fragments arranged by size:
3131 2931 360
RsrII CG'GwC_CG
Cuts at: 4617 4617
Size: 6422
SacI G_AGCT'C
Cuts at: 1854 2485 3436 1854
Size: 631 951 4840
Fragments arranged by size:
4840 951 631
SalI G'TCGA_C
Cuts at: 3459 3459
Size: 6422
SapI GCTCTTCn'nnn_
Cuts at: 4445 4655 4445
Size: 210 6212
SbfI CC_TGCA'GG
Cuts at: 3469 3469
Size: 6422
ScaI AGT'ACT
Cuts at: 1751 5939 1751
Size: 4188 2234
SfcI C'TryA_G
Cuts at: 33 371 554 1506 1619 1784 3465 4150 5143
Size: 338 183 952 113 165 1681 685 993
Cuts at: 5143 5334 33
Size: 191 1121
Fragments arranged by size:
1681 1121 993 952 685 338 191 183
165 113
SfoI GGC'GCC
Cuts at: 3854 4102 3854
Size: 248 6174
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6422
SmaI CCC'GGG
Cuts at: 120 3444 120
Size: 3324 3098
SmlI C'TyrA_G
Cuts at: 347 1670 1755 2428 2670 2863 3577 4492 4984
Size: 1323 85 673 242 193 714 915 492
Cuts at: 4984 5246 347
Size: 262 1523
Fragments arranged by size:
1523 1323 915 714 673 492 262 242
193 85
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6422
SphI G_CATG'C
Cuts at: 1119 2307 3475 3975 1119
Size: 1188 1168 500 3566
Fragments arranged by size:
3566 1188 1168 500
SspI AAT'ATT
Cuts at: 801 1462 801
Size: 661 5761
StyI C'CwwG_G
Cuts at: 107 1953 3358 3556 3910 107
Size: 1846 1405 198 354 2619
Fragments arranged by size:
2619 1846 1405 354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 293 4005 4138 4597 4806 293
Size: 3712 133 459 209 1909
Fragments arranged by size:
3712 1909 459 209 133
TatI w'GTAC_w
Cuts at: 1749 5937 1749
Size: 4188 2234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5939 5971 5939
Size: 32 6390
Tth111I GACn'n_nGTC
Cuts at: 4219 4219
Size: 6422
XbaI T'CTAG_A
Cuts at: 999 3306 3453 999
Size: 2307 147 3968
Fragments arranged by size:
3968 2307 147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 516 742 3917 516
Size: 226 3175 3021
Fragments arranged by size:
3175 3021 226
XmaI C'CCGG_G
Cuts at: 118 3442 118
Size: 3324 3098
XmnI GAAnn'nnTTC
Cuts at: 626 773 1183 2092 626
Size: 147 410 909 4956
Fragments arranged by size:
4956 909 410 147
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NspI PciI PflMI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsrGI BssHII BstEII BstZ17I CspCI DraIII FseI FspAI
NheI NotI NsiI PacI PasI PfoI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI