Restriction map for
pF1KB2048
(Circular) (Six-base) MAPSORT of: pF1KB2048.seq Check: 1428 from: 1 to: 6164
pF1KB2048 6164 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2930 3202 2930
Size: 272 5892
Acc65I G'GTAC_C
Cuts at: 2939 3180 2939
Size: 241 5923
AclI AA'CG_TT
Cuts at: 6004 6004
Size: 6164
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 2170 2290 3088 3989 4421 4774 5436 139
Size: 2031 120 798 901 432 353 662 867
Fragments arranged by size:
2031 901 867 798 662 432 353 120
AflIII A'CryG_T
Cuts at: 1048 3516 5316 5597 1048
Size: 2468 1800 281 1615
Fragments arranged by size:
2468 1800 1615 281
AlwNI CAG_nnn'CTG
Cuts at: 1344 1611 1669 3469 4907 1344
Size: 267 58 1800 1438 2601
Fragments arranged by size:
2601 1800 1438 267 58
ApaLI G'TGCA_C
Cuts at: 5002 5002
Size: 6164
ApoI r'AATT_y
Cuts at: 602 931 3168 5517 5773 602
Size: 329 2237 2349 256 993
Fragments arranged by size:
2349 2237 993 329 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6164
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6164
AvaI C'yCGr_G
Cuts at: 1560 1919 2793 3184 1560
Size: 359 874 391 4540
Fragments arranged by size:
4540 874 391 359
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1611 1644 1611
Size: 33 6131
BamHI G'GATC_C
Cuts at: 2318 3189 2318
Size: 871 5293
BanI G'GyrC_C
Cuts at: 905 1576 1645 2939 3180 3594 3842 3877 905
Size: 671 69 1294 241 414 248 35 3192
Fragments arranged by size:
3192 1294 671 414 248 241 69 35
BanII G_rGCy'C
Cuts at: 1381 1567 3178 4208 1381
Size: 186 1611 1030 3337
Fragments arranged by size:
3337 1611 1030 186
BbeI G_GCGC'C
Cuts at: 1649 3598 3846 1649
Size: 1949 248 3967
Fragments arranged by size:
3967 1949 248
BbsI GAAGACnn'nnnn_
Cuts at: 889 889
Size: 6164
BbvCI CC'TCA_GC
Cuts at: 1912 1912
Size: 6164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 414 448 2963 2997 414
Size: 34 2515 34 3581
Fragments arranged by size:
3581 2515 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4062 5118 6164 4062
Size: 1056 1046 4062
Fragments arranged by size:
4062 1056 1046
BclI T'GATC_A
Cuts at: 3684 3684
Size: 6164
BglII A'GATC_T
Cuts at: 2585 3679 5716 2585
Size: 1094 2037 3033
Fragments arranged by size:
3033 2037 1094
BlpI GC'TnA_GC
Cuts at: 790 1380 2260 3276 790
Size: 590 880 1016 3678
Fragments arranged by size:
3678 1016 880 590
Bme1580I G_kGCm'C
Cuts at: 908 1579 3789 3882 5006 908
Size: 671 2210 93 1124 2066
Fragments arranged by size:
2210 2066 1124 671 93
BmgBI CAC'GTC
Cuts at: 2346 2346
Size: 6164
BmrI ACTGGGnnnn_n'
Cuts at: 1529 2605 3004 3376 3457 3792 5936 1529
Size: 1076 399 372 81 335 2144 1757
Fragments arranged by size:
2144 1757 1076 399 372 335 81
BmtI G_CTAG'C
Cuts at: 547 547
Size: 6164
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1395 4531 1395
Size: 3136 3028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 230 238 423 1608 1951 2075 2173 218
Size: 12 8 185 1185 343 124 98 4209
Fragments arranged by size:
4209 1185 343 185 124 98 12 8
Bpu10I CC'TnA_GC
Cuts at: 1204 1912 1970 2754 1204
Size: 708 58 784 4614
Fragments arranged by size:
4614 784 708 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 1297 2020 2026 3340 4219 4933 5231 665
Size: 632 723 6 1314 879 714 298 1598
Fragments arranged by size:
1598 1314 879 723 714 632 298 6
BsaI GGTCTCn'nnnn_
Cuts at: 530 727 1916 2839 5868 530
Size: 197 1189 923 3029 826
Fragments arranged by size:
3029 1189 923 826 197
BsaAI yAC'GTr
Cuts at: 410 3517 410
Size: 3107 3057
BsaBI GATnn'nnATC
Cuts at: 6 3678 5715 6
Size: 3672 2037 455
Fragments arranged by size:
3672 2037 455
BsaHI Gr'CG_yC
Cuts at: 1646 2741 3595 3843 4545 6041 1646
Size: 1095 854 248 702 1496 1769
Fragments arranged by size:
1769 1496 1095 854 702 248
BsaWI w'CCGG_w
Cuts at: 488 908 3000 3353 3874 4552 4963 5110 5384
Size: 420 2092 353 521 678 411 147 274
Cuts at: 5384 488
Size: 1268
Fragments arranged by size:
2092 1268 678 521 420 411 353 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1722 1752 2107 2137 1722
Size: 30 355 30 5749
Fragments arranged by size:
5749 355 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1225 1228 2398 2914 1225
Size: 3 1170 516 4475
Fragments arranged by size:
4475 1170 516 3
BseYI C'CCAG_C
Cuts at: 421 1128 1274 3589 4347 5012 5420 421
Size: 707 146 2315 758 665 408 1165
Fragments arranged by size:
2315 1165 758 707 665 408 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2729 2857 2117
Size: 612 128 5424
Fragments arranged by size:
5424 612 128
BsiEI CG_ry'CG
Cuts at: 104 1063 3706 3752 4982 104
Size: 959 2643 46 1230 1286
Fragments arranged by size:
2643 1286 1230 959 46
BsiHKAI G_wGCw'C
Cuts at: 1845 2755 3178 3956 4146 5006 1845
Size: 910 423 778 190 860 3003
Fragments arranged by size:
3003 910 860 778 423 190
BsmI GAATG_Cn'
Cuts at: 588 1165 1226 1537 1731 2179 588
Size: 577 61 311 194 448 4573
Fragments arranged by size:
4573 577 448 311 194 61
BsmBI CGTCTCn'nnnn_
Cuts at: 2065 2293 2410 2065
Size: 228 117 5819
Fragments arranged by size:
5819 228 117
Bsp1286I G_dGCh'C
Cuts at: 908 1381 1567 1579 1845 2755 3178 3789 3882
Size: 473 186 12 266 910 423 611 93
Cuts at: 3882 3956 4146 4208 5006 908
Size: 74 190 62 798 2066
Fragments arranged by size:
2066 910 798 611 473 423 266 190
186 93 74 62 12
BspEI T'CCGG_A
Cuts at: 488 488
Size: 6164
BspHI T'CATG_A
Cuts at: 1262 4596 1262
Size: 3334 2830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1701 3214 3428 3730 4111 1701
Size: 1513 214 302 381 3754
Fragments arranged by size:
3754 1513 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1028 1821 4456 4510 5994 6159 50
Size: 978 793 2635 54 1484 165 55
Fragments arranged by size:
2635 1484 978 793 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2448 4076 2448
Size: 1628 4536
BsrFI r'CCGG_y
Cuts at: 4162 4343 4162
Size: 181 5983
BsrGI T'GTAC_A
Cuts at: 2213 2213
Size: 6164
BssSI C'ACGA_G
Cuts at: 2905 4435 5143 2905
Size: 1530 708 3926
Fragments arranged by size:
3926 1530 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3541 3541
Size: 6164
BstBI TT'CG_AA
Cuts at: 4525 4525
Size: 6164
BstEII G'GTnAC_C
Cuts at: 797 797
Size: 6164
BstXI CCAn_nnnn'nTGG
Cuts at: 1374 1374
Size: 6164
BstYI r'GATC_y
Cuts at: 1 2318 2585 3189 3656 3679 4014 4260 4566
Size: 2317 267 604 467 23 335 246 306
Cuts at: 4566 4578 4664 5716 1
Size: 12 86 1052 449
Fragments arranged by size:
2317 1052 604 467 449 335 306 267
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3148 3148
Size: 6164
BtgI C'CryG_G
Cuts at: 1532 1532
Size: 6164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 518 1964 4114 4295 4458 518
Size: 1446 2150 181 163 2224
Fragments arranged by size:
2224 2150 1446 181 163
BtsI GCAGTG_nn'
Cuts at: 472 851 1947 2324 3407 472
Size: 379 1096 377 1083 3229
Fragments arranged by size:
3229 1096 1083 379 377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3023 3058 3023
Size: 35 6129
DraI TTT'AAA
Cuts at: 3163 3163
Size: 6164
DraIII CAC_nnn'GTG
Cuts at: 1840 3125 1840
Size: 1285 4879
DrdI GACnn_nn'nnGTC
Cuts at: 3870 5214 3870
Size: 1344 4820
EaeI y'GGCC_r
Cuts at: 1060 1476 3749 3923 4314 4341 5686 1060
Size: 416 2273 174 391 27 1345 1538
Fragments arranged by size:
2273 1538 1345 416 391 174 27
EagI C'GGCC_G
Cuts at: 1060 3749 1060
Size: 2689 3475
EarI CTCTTCn'nnn_
Cuts at: 1213 1348 1593 2531 2821 3049 3121 4187 4397
Size: 135 245 938 290 228 72 1066 210
Cuts at: 4397 1213
Size: 2980
Fragments arranged by size:
2980 1066 938 290 245 228 210 135
72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4372 5114 5260 5974 4372
Size: 742 146 714 4562
Fragments arranged by size:
4562 742 714 146
EcoICRI GAG'CTC
Cuts at: 3176 3176
Size: 6164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 218 230 238 423 1608 1951 2075 2170
Size: 79 12 8 185 1185 343 124 95
Cuts at: 2170 2173 2290 3088 3989 4421 4774 5436 139
Size: 3 117 798 901 432 353 662 867
Fragments arranged by size:
1185 901 867 798 662 432 353 343
185 124 117 95 79 12 8 3
EcoNI CCTnn'n_nnAGG
Cuts at: 256 1207 256
Size: 951 5213
EcoO109I rG'GnC_Cy
Cuts at: 654 847 1916 3303 654
Size: 193 1069 1387 3515
Fragments arranged by size:
3515 1387 1069 193
EcoRI G'AATT_C
Cuts at: 3168 3168
Size: 6164
EcoRV GAT'ATC
Cuts at: 1428 1428
Size: 6164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4183 4183
Size: 6164
FspI TGC'GCA
Cuts at: 3945 3945
Size: 6164
HaeII r_GCGC'y
Cuts at: 1649 2476 3114 3598 3846 5076 5847 1649
Size: 827 638 484 248 1230 771 1966
Fragments arranged by size:
1966 1230 827 771 638 484 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 294 326 4006 4036 4038 4068 294
Size: 32 3680 30 2 30 2390
Fragments arranged by size:
3680 2390 32 30 30 2
HincII GTy'rAC
Cuts at: 135 647 1407 1766 3035 3203 135
Size: 512 760 359 1269 168 3096
Fragments arranged by size:
3096 1269 760 512 359 168
HindIII A'AGCT_T
Cuts at: 448 448
Size: 6164
HpaI GTT'AAC
Cuts at: 1766 1766
Size: 6164
KasI G'GCGC_C
Cuts at: 1645 3594 3842 1645
Size: 1949 248 3967
Fragments arranged by size:
3967 1949 248
KpnI G_GTAC'C
Cuts at: 2943 3184 2943
Size: 241 5923
MluI A'CGCG_T
Cuts at: 5597 5597
Size: 6164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 710 1891 2813 4923 5107 710
Size: 1181 922 2110 184 1767
Fragments arranged by size:
2110 1767 1181 922 184
MscI TGG'CCA
Cuts at: 3925 3925
Size: 6164
MslI CAynn'nnrTG
Cuts at: 2068 2221 4153 4280 2068
Size: 153 1932 127 3952
Fragments arranged by size:
3952 1932 153 127
MspA1I CmG'CkG
Cuts at: 794 1065 1344 1512 1873 2095 2339 2482 3275
Size: 271 279 168 361 222 244 143 793
Cuts at: 3275 3589 3949 4733 4978 794
Size: 314 360 784 245 1980
Fragments arranged by size:
1980 793 784 361 360 314 279 271
245 244 222 168 143
NaeI GCC'GGC
Cuts at: 4345 4345
Size: 6164
NarI GG'CG_CC
Cuts at: 1646 3595 3843 1646
Size: 1949 248 3967
Fragments arranged by size:
3967 1949 248
NgoMIV G'CCGG_C
Cuts at: 4343 4343
Size: 6164
NheI G'CTAG_C
Cuts at: 543 543
Size: 6164
NspI r_CATG'y
Cuts at: 323 1052 3217 3717 5320 323
Size: 729 2165 500 1603 1167
Fragments arranged by size:
2165 1603 1167 729 500
PciI A'CATG_T
Cuts at: 1048 5316 1048
Size: 4268 1896
PfoI T'CCnGG_A
Cuts at: 254 1111 1928 254
Size: 857 817 4490
Fragments arranged by size:
4490 857 817
PmeI GTTT'AAAC
Cuts at: 3163 3163
Size: 6164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4610 4642 4610
Size: 32 6132
PpuMI rG'GwC_Cy
Cuts at: 654 654
Size: 6164
PshAI GACnn'nnGTC
Cuts at: 130 2057 130
Size: 1927 4237
PstI C_TGCA'G
Cuts at: 1698 2487 2897 3211 3896 1698
Size: 789 410 314 685 3966
Fragments arranged by size:
3966 789 685 410 314
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6164
PvuII CAG'CTG
Cuts at: 794 1344 1512 1873 2095 2339 3589 3949 794
Size: 550 168 361 222 244 1250 360 3009
Fragments arranged by size:
3009 1250 550 361 360 244 222 168
RsrII CG'GwC_CG
Cuts at: 4359 4359
Size: 6164
SacI G_AGCT'C
Cuts at: 3178 3178
Size: 6164
SalI G'TCGA_C
Cuts at: 3201 3201
Size: 6164
SanDI GG'GwC_CC
Cuts at: 654 654
Size: 6164
SapI GCTCTTCn'nnn_
Cuts at: 1213 2821 4187 4397 1213
Size: 1608 1366 210 2980
Fragments arranged by size:
2980 1608 1366 210
SbfI CC_TGCA'GG
Cuts at: 1698 3211 1698
Size: 1513 4651
ScaI AGT'ACT
Cuts at: 5681 5681
Size: 6164
SexAI A'CCwGG_T
Cuts at: 815 1836 2672 2870 815
Size: 1021 836 198 4109
Fragments arranged by size:
4109 1021 836 198
SfcI C'TryA_G
Cuts at: 33 1694 2483 2893 3207 3892 4860 5051 33
Size: 1661 789 410 314 685 968 191 1146
Fragments arranged by size:
1661 1146 968 789 685 410 314 191
SfoI GGC'GCC
Cuts at: 1647 3596 3844 1647
Size: 1949 248 3967
Fragments arranged by size:
3967 1949 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6164
SmaI CCC'GGG
Cuts at: 3186 3186
Size: 6164
SmlI C'TyrA_G
Cuts at: 680 1312 1999 2005 3319 4234 4948 5210 680
Size: 632 687 6 1314 915 714 262 1634
Fragments arranged by size:
1634 1314 915 714 687 632 262 6
SnaBI TAC'GTA
Cuts at: 410 410
Size: 6164
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6164
SphI G_CATG'C
Cuts at: 3217 3717 3217
Size: 500 5664
SspI AAT'ATT
Cuts at: 282 282
Size: 6164
StyI C'CwwG_G
Cuts at: 3298 3652 3298
Size: 354 5810
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1282 3747 3880 4339 4548 1282
Size: 2465 133 459 209 2898
Fragments arranged by size:
2898 2465 459 209 133
TatI w'GTAC_w
Cuts at: 2213 5679 2213
Size: 3466 2698
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2107 2139 5681 5713 2107
Size: 32 3542 32 2558
Fragments arranged by size:
3542 2558 32 32
Tth111I GACn'n_nGTC
Cuts at: 1773 3961 1773
Size: 2188 3976
XbaI T'CTAG_A
Cuts at: 3195 3195
Size: 6164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1189 1240 2670 3659 1189
Size: 51 1430 989 3694
Fragments arranged by size:
3694 1430 989 51
XmaI C'CCGG_G
Cuts at: 3184 3184
Size: 6164
XmnI GAAnn'nnTTC
Cuts at: 281 281
Size: 6164
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PciI
PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BfrBI BglI BsiWI BssHII
BstZ17I ClaI FseI FspAI MfeI NcoI NdeI NotI
NruI NsiI PacI PasI PflMI PmlI PsiI PspOMI
PspXI PsrI SacII SfiI SgrAI SrfI StuI SwaI
XhoI ZraI