Restriction map for
pF1KB2323
(Circular) (Six-base) MAPSORT of: pF1KB2323.seq Check: 5667 from: 1 to: 6176
pF1KB2323 6176 bp
With 182 enzymes: SgfI *
July 25, 2014 11:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1269 2759 1269
Size: 1490 4686
AatII G_ACGT'C
Cuts at: 389 389
Size: 6176
AccI GT'mk_AC
Cuts at: 389 745 1354 2464 3214 389
Size: 356 609 1110 750 3351
Fragments arranged by size:
3351 1110 750 609 356
Acc65I G'GTAC_C
Cuts at: 659 1536 3192 659
Size: 877 1656 3643
Fragments arranged by size:
3643 1656 877
AclI AA'CG_TT
Cuts at: 3170 6016 3170
Size: 2846 3330
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 1013 1138 1270 2212 2809 2912 4001 4433
Size: 783 125 132 942 597 103 1089 432
Cuts at: 4433 4786 5448 230
Size: 353 662 958
Fragments arranged by size:
1089 958 942 783 662 597 432 353
132 125 103
AflIII A'CryG_T
Cuts at: 2547 3528 5328 5609 2547
Size: 981 1800 281 3114
Fragments arranged by size:
3114 1800 981 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1538 1538
Size: 6176
AlwNI CAG_nnn'CTG
Cuts at: 1705 2954 3481 4919 1705
Size: 1249 527 1438 2962
Fragments arranged by size:
2962 1438 1249 527
ApaI G_GGCC'C
Cuts at: 740 1213 740
Size: 473 5703
ApaLI G'TGCA_C
Cuts at: 1315 2695 5014 1315
Size: 1380 2319 2477
Fragments arranged by size:
2477 2319 1380
ApoI r'AATT_y
Cuts at: 1920 2049 2142 2356 3180 5529 5785 1920
Size: 129 93 214 824 2349 256 2311
Fragments arranged by size:
2349 2311 824 256 214 129 93
AseI AT'TA_AT
Cuts at: 20 2879 20
Size: 2859 3317
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6176
AvaI C'yCGr_G
Cuts at: 1477 2073 3196 1477
Size: 596 1123 4457
Fragments arranged by size:
4457 1123 596
BamHI G'GATC_C
Cuts at: 876 935 3201 876
Size: 59 2266 3851
Fragments arranged by size:
3851 2266 59
BanI G'GyrC_C
Cuts at: 659 1225 1522 1536 1824 2747 3192 3606 3854
Size: 566 297 14 288 923 445 414 248
Cuts at: 3854 3889 659
Size: 35 2946
Fragments arranged by size:
2946 923 566 445 414 297 288 248
35 14
BanII G_rGCy'C
Cuts at: 740 1157 1213 2168 2741 2936 3190 4220 740
Size: 417 56 955 573 195 254 1030 2696
Fragments arranged by size:
2696 1030 955 573 417 254 195 56
BbeI G_GCGC'C
Cuts at: 1229 3610 3858 1229
Size: 2381 248 3547
Fragments arranged by size:
3547 2381 248
BbsI GAAGACnn'nnnn_
Cuts at: 1951 2420 3152 1951
Size: 469 732 4975
Fragments arranged by size:
4975 732 469
BciVI GTATCCnnnnn_n'
Cuts at: 4074 5130 6176 4074
Size: 1056 1046 4074
Fragments arranged by size:
4074 1056 1046
BclI T'GATC_A
Cuts at: 3696 3696
Size: 6176
BglII A'GATC_T
Cuts at: 1196 1674 3691 5728 1196
Size: 478 2017 2037 1644
Fragments arranged by size:
2037 2017 1644 478
BlpI GC'TnA_GC
Cuts at: 157 2631 3288 157
Size: 2474 657 3045
Fragments arranged by size:
3045 2474 657
Bme1580I G_kGCm'C
Cuts at: 740 1213 1319 2699 3801 3894 5018 740
Size: 473 106 1380 1102 93 1124 1898
Fragments arranged by size:
1898 1380 1124 1102 473 106 93
BmgBI CAC'GTC
Cuts at: 1841 1841
Size: 6176
BmrI ACTGGGnnnn_n'
Cuts at: 214 796 1535 1587 2330 3388 3469 3804 5948
Size: 582 739 52 743 1058 81 335 2144
Cuts at: 5948 214
Size: 442
Fragments arranged by size:
2144 1058 743 739 582 442 335 81
52
BmtI G_CTAG'C
Cuts at: 1862 1862
Size: 6176
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1411 1703 4543 1411
Size: 292 2840 3044
Fragments arranged by size:
3044 2840 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 808 1141 1585 1615 1738 2311 2635 2740
Size: 384 333 444 30 123 573 324 105
Cuts at: 2740 3140 424
Size: 400 3460
Fragments arranged by size:
3460 573 444 400 384 333 324 123
105 30
Bpu10I CC'TnA_GC
Cuts at: 2740 2740
Size: 6176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 1804 2509 3352 4231 4945 5243 1177
Size: 627 705 843 879 714 298 2110
Fragments arranged by size:
2110 879 843 714 705 627 298
BsaI GGTCTCn'nnnn_
Cuts at: 5880 5880
Size: 6176
BsaAI yAC'GTr
Cuts at: 393 1314 2548 3529 393
Size: 921 1234 981 3040
Fragments arranged by size:
3040 1234 981 921
BsaBI GATnn'nnATC
Cuts at: 6 898 3690 5727 6
Size: 892 2792 2037 455
Fragments arranged by size:
2792 2037 892 455
BsaHI Gr'CG_yC
Cuts at: 386 1226 3607 3855 4557 6053 386
Size: 840 2381 248 702 1496 509
Fragments arranged by size:
2381 1496 840 702 509 248
BsaWI w'CCGG_w
Cuts at: 3365 3886 4564 4975 5122 5396 3365
Size: 521 678 411 147 274 4145
Fragments arranged by size:
4145 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 692 2949 2998 3019 3166 692
Size: 2257 49 21 147 3702
Fragments arranged by size:
3702 2257 147 49 21
BseYI C'CCAG_C
Cuts at: 1518 3601 4359 5024 5432 1518
Size: 2083 758 665 408 2262
Fragments arranged by size:
2262 2083 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1534 1659 2380 2737 1534
Size: 125 721 357 4973
Fragments arranged by size:
4973 721 357 125
BsiEI CG_ry'CG
Cuts at: 104 2424 3718 3764 4994 104
Size: 2320 1294 46 1230 1286
Fragments arranged by size:
2320 1294 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1157 1319 2168 2588 2699 2915 3190 3968 4158
Size: 162 849 420 111 216 275 778 190
Cuts at: 4158 5018 1157
Size: 860 2315
Fragments arranged by size:
2315 860 849 778 420 275 216 190
162 111
BsmBI CGTCTCn'nnnn_
Cuts at: 377 377
Size: 6176
Bsp1286I G_dGCh'C
Cuts at: 740 1157 1213 1319 2168 2588 2699 2741 2915
Size: 417 56 106 849 420 111 42 174
Cuts at: 2915 2936 3190 3801 3894 3968 4158 4220 5018
Size: 21 254 611 93 74 190 62 798
Cuts at: 5018 740
Size: 1898
Fragments arranged by size:
1898 849 798 611 420 417 254 190
174 111 106 93 74 62 56 42
21
BspHI T'CATG_A
Cuts at: 1253 4608 1253
Size: 3355 2821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 299 1269 2759 3226 3440 3742 4123 299
Size: 970 1490 467 214 302 381 2352
Fragments arranged by size:
2352 1490 970 467 381 302 214
BsrBI CCG'CTC
Cuts at: 50 753 4468 4522 6006 6171 50
Size: 703 3715 54 1484 165 55
Fragments arranged by size:
3715 1484 703 165 55 54
BsrDI GCAATG_nn'
Cuts at: 243 4088 243
Size: 3845 2331
BsrFI r'CCGG_y
Cuts at: 1804 4174 4355 1804
Size: 2370 181 3625
Fragments arranged by size:
3625 2370 181
BssSI C'ACGA_G
Cuts at: 4447 5155 4447
Size: 708 5468
BstAPI GCAn_nnn'nTGC
Cuts at: 233 372 3553 233
Size: 139 3181 2856
Fragments arranged by size:
3181 2856 139
BstBI TT'CG_AA
Cuts at: 1887 4537 1887
Size: 2650 3526
BstEII G'GTnAC_C
Cuts at: 214 214
Size: 6176
BstXI CCAn_nnnn'nTGG
Cuts at: 2873 2873
Size: 6176
BstYI r'GATC_y
Cuts at: 1 876 935 1196 1674 2285 2951 3201 3668
Size: 875 59 261 478 611 666 250 467
Cuts at: 3668 3691 4026 4272 4578 4590 4676 5728 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 875 666 611 478 467 449 335
306 261 250 246 86 59 23 12
BstZ17I GTA'TAC
Cuts at: 746 746
Size: 6176
Bsu36I CC'TnA_GG
Cuts at: 1321 1321
Size: 6176
BtgI C'CryG_G
Cuts at: 107 1459 1548 2856 107
Size: 1352 89 1308 3427
Fragments arranged by size:
3427 1352 1308 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4126 4307 4470 4126
Size: 181 163 5832
Fragments arranged by size:
5832 181 163
BtsI GCAGTG_nn'
Cuts at: 953 1038 1607 3064 3419 953
Size: 85 569 1457 355 3710
Fragments arranged by size:
3710 1457 569 355 85
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1325 1360 1395 1325
Size: 35 35 6106
Fragments arranged by size:
6106 35 35
DraI TTT'AAA
Cuts at: 3175 3175
Size: 6176
DrdI GACnn_nn'nnGTC
Cuts at: 1953 3882 5226 1953
Size: 1929 1344 2903
Fragments arranged by size:
2903 1929 1344
EaeI y'GGCC_r
Cuts at: 165 246 1035 2597 3761 3935 4326 4353 5698
Size: 81 789 1562 1164 174 391 27 1345
Cuts at: 5698 165
Size: 643
Fragments arranged by size:
1562 1345 1164 789 643 391 174 81
27
EagI C'GGCC_G
Cuts at: 3761 3761
Size: 6176
EarI CTCTTCn'nnn_
Cuts at: 2000 2726 4199 4409 2000
Size: 726 1473 210 3767
Fragments arranged by size:
3767 1473 726 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4384 5126 5272 5986 4384
Size: 742 146 714 4574
Fragments arranged by size:
4574 742 714 146
EcoICRI GAG'CTC
Cuts at: 1155 2166 3188 1155
Size: 1011 1022 4143
Fragments arranged by size:
4143 1022 1011
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 424 808 1013 1138 1141 1270 1585 1615
Size: 194 384 205 125 3 129 315 30
Cuts at: 1615 1738 2212 2311 2635 2740 2809 2912 3140
Size: 123 474 99 324 105 69 103 228
Cuts at: 3140 4001 4433 4786 5448 230
Size: 861 432 353 662 958
Fragments arranged by size:
958 861 662 474 432 384 353 324
315 228 205 194 129 125 123 105
103 99 69 30 3
EcoNI CCTnn'n_nnAGG
Cuts at: 667 667
Size: 6176
EcoO109I rG'GnC_Cy
Cuts at: 256 737 790 1174 3315 256
Size: 481 53 384 2141 3117
Fragments arranged by size:
3117 2141 481 384 53
EcoRI G'AATT_C
Cuts at: 2142 3180 2142
Size: 1038 5138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4195 4195
Size: 6176
FspI TGC'GCA
Cuts at: 3957 3957
Size: 6176
HaeII r_GCGC'y
Cuts at: 1229 3610 3858 5088 5859 1229
Size: 2381 248 1230 771 1546
Fragments arranged by size:
2381 1546 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 532 564 690 722 861 893 1128 1160 1188
Size: 32 126 32 139 32 235 32 28
Cuts at: 1188 1220 1734 1766 4018 4048 4050 4080 532
Size: 32 514 32 2252 30 2 30 2628
Fragments arranged by size:
2628 2252 514 235 139 126 32 32
32 32 32 32 30 30 28 2
HincII GTy'rAC
Cuts at: 966 1769 3062 3215 966
Size: 803 1293 153 3927
Fragments arranged by size:
3927 1293 803 153
HindIII A'AGCT_T
Cuts at: 2485 2791 2485
Size: 306 5870
HpaI GTT'AAC
Cuts at: 1769 1769
Size: 6176
KasI G'GCGC_C
Cuts at: 1225 3606 3854 1225
Size: 2381 248 3547
Fragments arranged by size:
3547 2381 248
KpnI G_GTAC'C
Cuts at: 663 1540 3196 663
Size: 877 1656 3643
Fragments arranged by size:
3643 1656 877
MluI A'CGCG_T
Cuts at: 5609 5609
Size: 6176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2204 3098 4935 5119 2204
Size: 894 1837 184 3261
Fragments arranged by size:
3261 1837 894 184
MscI TGG'CCA
Cuts at: 167 248 2599 3937 167
Size: 81 2351 1338 2406
Fragments arranged by size:
2406 2351 1338 81
MslI CAynn'nnrTG
Cuts at: 1094 1635 4165 4292 1094
Size: 541 2530 127 2978
Fragments arranged by size:
2978 2530 541 127
MspA1I CmG'CkG
Cuts at: 369 846 976 1518 1593 2394 2606 2904 3287
Size: 477 130 542 75 801 212 298 383
Cuts at: 3287 3601 3961 4745 4990 369
Size: 314 360 784 245 1555
Fragments arranged by size:
1555 801 784 542 477 383 360 314
298 245 212 130 75
NaeI GCC'GGC
Cuts at: 4357 4357
Size: 6176
NarI GG'CG_CC
Cuts at: 1226 3607 3855 1226
Size: 2381 248 3547
Fragments arranged by size:
3547 2381 248
NcoI C'CATG_G
Cuts at: 107 1548 2856 107
Size: 1441 1308 3427
Fragments arranged by size:
3427 1441 1308
NdeI CA'TA_TG
Cuts at: 123 1632 123
Size: 1509 4667
NgoMIV G'CCGG_C
Cuts at: 4355 4355
Size: 6176
NheI G'CTAG_C
Cuts at: 1858 1858
Size: 6176
NspI r_CATG'y
Cuts at: 3229 3729 5332 3229
Size: 500 1603 4073
Fragments arranged by size:
4073 1603 500
PasI CC'CwG_GG
Cuts at: 733 733
Size: 6176
PciI A'CATG_T
Cuts at: 5328 5328
Size: 6176
PflMI CCAn_nnn'nTGG
Cuts at: 2243 2243
Size: 6176
PfoI T'CCnGG_A
Cuts at: 2288 2947 2288
Size: 659 5517
PmeI GTTT'AAAC
Cuts at: 3175 3175
Size: 6176
PmlI CAC'GTG
Cuts at: 1314 1314
Size: 6176
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4622 4654 4622
Size: 32 6144
PpuMI rG'GwC_Cy
Cuts at: 256 790 256
Size: 534 5642
PsiI TTA'TAA
Cuts at: 2314 2314
Size: 6176
PspOMI G'GGCC_C
Cuts at: 736 1209 736
Size: 473 5703
PstI C_TGCA'G
Cuts at: 2611 3223 3908 2611
Size: 612 685 4879
Fragments arranged by size:
4879 685 612
PvuI CG_AT'CG
Cuts at: 104 2424 104
Size: 2320 3856
PvuII CAG'CTG
Cuts at: 846 976 1518 1593 2394 3601 3961 846
Size: 130 542 75 801 1207 360 3061
Fragments arranged by size:
3061 1207 801 542 360 130 75
RsrII CG'GwC_CG
Cuts at: 4371 4371
Size: 6176
SacI G_AGCT'C
Cuts at: 1157 2168 3190 1157
Size: 1011 1022 4143
Fragments arranged by size:
4143 1022 1011
SalI G'TCGA_C
Cuts at: 3213 3213
Size: 6176
SanDI GG'GwC_CC
Cuts at: 790 790
Size: 6176
SapI GCTCTTCn'nnn_
Cuts at: 4199 4409 4199
Size: 210 5966
SbfI CC_TGCA'GG
Cuts at: 3223 3223
Size: 6176
ScaI AGT'ACT
Cuts at: 5693 5693
Size: 6176
SexAI A'CCwGG_T
Cuts at: 655 655
Size: 6176
SfcI C'TryA_G
Cuts at: 33 1230 2607 2774 3219 3904 4872 5063 33
Size: 1197 1377 167 445 685 968 191 1146
Fragments arranged by size:
1377 1197 1146 968 685 445 191 167
SfoI GGC'GCC
Cuts at: 1227 3608 3856 1227
Size: 2381 248 3547
Fragments arranged by size:
3547 2381 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6176
SmaI CCC'GGG
Cuts at: 3198 3198
Size: 6176
SmlI C'TyrA_G
Cuts at: 1192 1819 2524 3331 4246 4960 5222 1192
Size: 627 705 807 915 714 262 2146
Fragments arranged by size:
2146 915 807 714 705 627 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6176
SphI G_CATG'C
Cuts at: 3229 3729 3229
Size: 500 5676
StyI C'CwwG_G
Cuts at: 107 332 1178 1548 2856 3310 3664 107
Size: 225 846 370 1308 454 354 2619
Fragments arranged by size:
2619 1308 846 454 370 354 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 931 1510 1909 2111 2584 2879 3090 3759 3892
Size: 579 399 202 473 295 211 669 133
Cuts at: 3892 4351 4560 931
Size: 459 209 2547
Fragments arranged by size:
2547 669 579 473 459 399 295 211
209 202 133
TatI w'GTAC_w
Cuts at: 2561 2642 2825 5691 2561
Size: 81 183 2866 3046
Fragments arranged by size:
3046 2866 183 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5693 5725 5693
Size: 32 6144
Tth111I GACn'n_nGTC
Cuts at: 2782 3973 2782
Size: 1191 4985
XbaI T'CTAG_A
Cuts at: 3207 3207
Size: 6176
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 887 1590 3671 887
Size: 703 2081 3392
Fragments arranged by size:
3392 2081 703
XmaI C'CCGG_G
Cuts at: 3196 3196
Size: 6176
XmnI GAAnn'nnTTC
Cuts at: 869 869
Size: 6176
ZraI GAC'GTC
Cuts at: 387 387
Size: 6176
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BsaXI BsiWI BsmI
BspEI BsrGI BssHII ClaI DraIII EcoRV FseI FspAI
MfeI NotI NruI NsiI PacI PshAI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI