Restriction map for
pF1KB2497
(Circular) (Six-base) MAPSORT of: pF1KB2497.seq Check: 6718 from: 1 to: 5522
pF1KB2497 5522 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 989 1088 1448 1730 989
Size: 99 360 282 4781
Fragments arranged by size:
4781 360 282 99
AccI GT'mk_AC
Cuts at: 2560 2560
Size: 5522
Acc65I G'GTAC_C
Cuts at: 2538 2538
Size: 5522
AclI AA'CG_TT
Cuts at: 5362 5362
Size: 5522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 3347 3779 4132 4794 991
Size: 2356 432 353 662 1719
Fragments arranged by size:
2356 1719 662 432 353
AflII C'TTAA_G
Cuts at: 1521 1521
Size: 5522
AflIII A'CryG_T
Cuts at: 2874 4674 4955 2874
Size: 1800 281 3441
Fragments arranged by size:
3441 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2094 2094
Size: 5522
AleI CACnn'nnGTG
Cuts at: 770 1147 770
Size: 377 5145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2069 2069
Size: 5522
AlwNI CAG_nnn'CTG
Cuts at: 451 633 1374 2827 4265 451
Size: 182 741 1453 1438 1708
Fragments arranged by size:
1708 1453 1438 741 182
ApaI G_GGCC'C
Cuts at: 256 272 313 481 524 862 880 256
Size: 16 41 168 43 338 18 4898
Fragments arranged by size:
4898 338 168 43 41 18 16
ApaLI G'TGCA_C
Cuts at: 976 4360 976
Size: 3384 2138
ApoI r'AATT_y
Cuts at: 1433 1863 2526 4875 5131 1433
Size: 430 663 2349 256 1824
Fragments arranged by size:
2349 1824 663 430 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5522
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
AvaI C'yCGr_G
Cuts at: 271 480 721 768 928 2542 271
Size: 209 241 47 160 1614 3251
Fragments arranged by size:
3251 1614 241 209 160 47
BamHI G'GATC_C
Cuts at: 2547 2547
Size: 5522
BanI G'GyrC_C
Cuts at: 330 837 1076 2045 2538 2952 3200 3235 330
Size: 507 239 969 493 414 248 35 2617
Fragments arranged by size:
2617 969 507 493 414 248 239 35
BanII G_rGCy'C
Cuts at: 256 272 313 433 481 488 524 862 880
Size: 16 41 120 48 7 36 338 18
Cuts at: 880 898 2032 2182 2536 3566 256
Size: 18 1134 150 354 1030 2212
Fragments arranged by size:
2212 1134 1030 354 338 150 120 48
41 36 18 18 16 7
BbeI G_GCGC'C
Cuts at: 334 841 2956 3204 334
Size: 507 2115 248 2652
Fragments arranged by size:
2652 2115 507 248
BbsI GAAGACnn'nnnn_
Cuts at: 1318 1807 1318
Size: 489 5033
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1248 1282 1248
Size: 34 5488
BciVI GTATCCnnnnn_n'
Cuts at: 178 2127 3420 4476 5522 178
Size: 1949 1293 1056 1046 178
Fragments arranged by size:
1949 1293 1056 1046 178
BclI T'GATC_A
Cuts at: 3042 3042
Size: 5522
BglI GCCn_nnn'nGGC
Cuts at: 276 298 276
Size: 22 5500
BglII A'GATC_T
Cuts at: 2481 3037 5074 2481
Size: 556 2037 2929
Fragments arranged by size:
2929 2037 556
BlpI GC'TnA_GC
Cuts at: 809 1847 2634 809
Size: 1038 787 3697
Fragments arranged by size:
3697 1038 787
Bme1580I G_kGCm'C
Cuts at: 256 272 313 481 524 610 862 880 928
Size: 16 41 168 43 86 252 18 48
Cuts at: 928 980 1567 1639 2048 3147 3240 4364 256
Size: 52 587 72 409 1099 93 1124 1414
Fragments arranged by size:
1414 1124 1099 587 409 252 168 93
86 72 52 48 43 41 18 16
BmgBI CAC'GTC
Cuts at: 417 417
Size: 5522
BmrI ACTGGGnnnn_n'
Cuts at: 2424 2734 2815 3150 5294 2424
Size: 310 81 335 2144 2652
Fragments arranged by size:
2652 2144 335 310 81
BmtI G_CTAG'C
Cuts at: 808 808
Size: 5522
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2051 3889 2051
Size: 1838 3684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 2241 472
Size: 1769 3753
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1144 2187 2698 3577 4291 4589 1054
Size: 90 1043 511 879 714 298 1987
Fragments arranged by size:
1987 1043 879 714 511 298 90
BsaI GGTCTCn'nnnn_
Cuts at: 415 1455 2205 5226 415
Size: 1040 750 3021 711
Fragments arranged by size:
3021 1040 750 711
BsaAI yAC'GTr
Cuts at: 2875 2875
Size: 5522
BsaBI GATnn'nnATC
Cuts at: 6 3036 5073 6
Size: 3030 2037 455
Fragments arranged by size:
3030 2037 455
BsaHI Gr'CG_yC
Cuts at: 331 656 838 2953 3201 3903 5399 331
Size: 325 182 2115 248 702 1496 454
Fragments arranged by size:
2115 1496 702 454 325 248 182
BsaWI w'CCGG_w
Cuts at: 433 1550 2711 3232 3910 4321 4468 4742 433
Size: 1117 1161 521 678 411 147 274 1213
Fragments arranged by size:
1213 1161 1117 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1693 1723 1693
Size: 30 5492
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 301 478 487 859 862 1231 1752 2111 301
Size: 177 9 372 3 369 521 359 3712
Fragments arranged by size:
3712 521 372 369 359 177 9 3
BseYI C'CCAG_C
Cuts at: 713 745 891 1876 2284 2947 3705 4370 4778
Size: 32 146 985 408 663 758 665 408
Cuts at: 4778 713
Size: 1457
Fragments arranged by size:
1457 985 758 665 663 408 408 146
32
BsiEI CG_ry'CG
Cuts at: 104 186 818 866 3064 3110 4340 104
Size: 82 632 48 2198 46 1230 1286
Fragments arranged by size:
2198 1286 1230 632 82 48 46
BsiHKAI G_wGCw'C
Cuts at: 980 1335 2032 2182 2332 2536 3314 3504 4364
Size: 355 697 150 150 204 778 190 860
Cuts at: 4364 980
Size: 2138
Fragments arranged by size:
2138 860 778 697 355 204 190 150
150
Bsp1286I G_dGCh'C
Cuts at: 256 272 313 433 481 488 524 610 862
Size: 16 41 120 48 7 36 86 252
Cuts at: 862 880 898 928 980 1335 1567 1639 2032
Size: 18 18 30 52 355 232 72 393
Cuts at: 2032 2048 2182 2332 2536 3147 3240 3314 3504
Size: 16 134 150 204 611 93 74 190
Cuts at: 3504 3566 4364 256
Size: 62 798 1414
Fragments arranged by size:
1414 798 611 393 355 252 232 204
190 150 134 120 93 86 74 72
62 52 48 41 36 30 18 18
16 16 7
BspHI T'CATG_A
Cuts at: 3954 3954
Size: 5522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 641 989 1088 1448 1730 2572 2786 3088 3469
Size: 348 99 360 282 842 214 302 381
Cuts at: 3469 641
Size: 2694
Fragments arranged by size:
2694 842 381 360 348 302 282 214
99
BsrBI CCG'CTC
Cuts at: 50 503 706 3814 3868 5352 5517 50
Size: 453 203 3108 54 1484 165 55
Fragments arranged by size:
3108 1484 453 203 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1969 3434 1969
Size: 1465 4057
BsrFI r'CCGG_y
Cuts at: 210 813 1453 3520 3701 210
Size: 603 640 2067 181 2031
Fragments arranged by size:
2067 2031 640 603 181
BsrGI T'GTAC_A
Cuts at: 1151 1151
Size: 5522
BssSI C'ACGA_G
Cuts at: 1593 3793 4501 1593
Size: 2200 708 2614
Fragments arranged by size:
2614 2200 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2899 2899
Size: 5522
BstBI TT'CG_AA
Cuts at: 1268 3883 1268
Size: 2615 2907
BstXI CCAn_nnnn'nTGG
Cuts at: 689 689
Size: 5522
BstYI r'GATC_y
Cuts at: 1 1836 2227 2481 2547 3014 3037 3372 3618
Size: 1835 391 254 66 467 23 335 246
Cuts at: 3618 3924 3936 4022 5074 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1835 1052 467 449 391 335 306 254
246 86 66 23 12
Bsu36I CC'TnA_GG
Cuts at: 842 2270 842
Size: 1428 4094
BtgI C'CryG_G
Cuts at: 107 173 425 515 551 593 882 2460 107
Size: 66 252 90 36 42 289 1578 3169
Fragments arranged by size:
3169 1578 289 252 90 66 42 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3472 3653 3816 3472
Size: 181 163 5178
Fragments arranged by size:
5178 181 163
BtsI GCAGTG_nn'
Cuts at: 524 943 1887 2765 524
Size: 419 944 878 3281
Fragments arranged by size:
3281 944 878 419
DraI TTT'AAA
Cuts at: 2521 2521
Size: 5522
DrdI GACnn_nn'nnGTC
Cuts at: 3228 4572 3228
Size: 1344 4178
EaeI y'GGCC_r
Cuts at: 183 216 668 815 863 2105 2159 2243 3107
Size: 33 452 147 48 1242 54 84 864
Cuts at: 3107 3281 3672 3699 5044 183
Size: 174 391 27 1345 661
Fragments arranged by size:
1345 1242 864 661 452 391 174 147
84 54 48 33 27
EagI C'GGCC_G
Cuts at: 183 815 863 3107 183
Size: 632 48 2244 2598
Fragments arranged by size:
2598 2244 632 48
EarI CTCTTCn'nnn_
Cuts at: 1783 1942 3545 3755 1783
Size: 159 1603 210 3550
Fragments arranged by size:
3550 1603 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 567 868 1615 3730 4472 4618 5332 567
Size: 301 747 2115 742 146 714 757
Fragments arranged by size:
2115 757 747 742 714 301 146
EcoICRI GAG'CTC
Cuts at: 2030 2180 2534 2030
Size: 150 354 5018
Fragments arranged by size:
5018 354 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 991 2241 3347 3779 4132 4794 472
Size: 519 1250 1106 432 353 662 1200
Fragments arranged by size:
1250 1200 1106 662 519 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1571 1661 1571
Size: 90 5432
EcoO109I rG'GnC_Cy
Cuts at: 199 222 252 253 309 310 448 477 520
Size: 23 30 1 56 1 138 29 43
Cuts at: 520 535 630 858 877 1013 2661 199
Size: 15 95 228 19 136 1648 3060
Fragments arranged by size:
3060 1648 228 138 136 95 56 43
30 29 23 19 15 1 1
EcoRI G'AATT_C
Cuts at: 2526 2526
Size: 5522
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3541 3541
Size: 5522
FspI TGC'GCA
Cuts at: 3303 3303
Size: 5522
HaeII r_GCGC'y
Cuts at: 334 841 1250 2956 3204 4434 5205 334
Size: 507 409 1706 248 1230 771 651
Fragments arranged by size:
1706 1230 771 651 507 409 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 327 359 1915 1917 1947 1949 2080 2112 2185
Size: 32 1556 2 30 2 131 32 73
Cuts at: 2185 2217 3364 3394 3396 3426 327
Size: 32 1147 30 2 30 2423
Fragments arranged by size:
2423 1556 1147 131 73 32 32 32
30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 2561 2561
Size: 5522
KasI G'GCGC_C
Cuts at: 330 837 2952 3200 330
Size: 507 2115 248 2652
Fragments arranged by size:
2652 2115 507 248
KpnI G_GTAC'C
Cuts at: 2542 2542
Size: 5522
MluI A'CGCG_T
Cuts at: 4955 4955
Size: 5522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4281 4465 4281
Size: 184 5338
MscI TGG'CCA
Cuts at: 218 670 2107 2245 3283 218
Size: 452 1437 138 1038 2457
Fragments arranged by size:
2457 1437 1038 452 138
MslI CAynn'nnrTG
Cuts at: 770 1147 1200 1507 1544 3511 3638 770
Size: 377 53 307 37 1967 127 2654
Fragments arranged by size:
2654 1967 377 307 127 53 37
MspA1I CmG'CkG
Cuts at: 175 427 517 542 553 595 884 891 969
Size: 252 90 25 11 42 289 7 78
Cuts at: 969 2633 2947 3307 4091 4336 175
Size: 1664 314 360 784 245 1361
Fragments arranged by size:
1664 1361 784 360 314 289 252 245
90 78 42 25 11 7
NaeI GCC'GGC
Cuts at: 212 815 3703 212
Size: 603 2888 2031
Fragments arranged by size:
2888 2031 603
NarI GG'CG_CC
Cuts at: 331 838 2953 3201 331
Size: 507 2115 248 2652
Fragments arranged by size:
2652 2115 507 248
NcoI C'CATG_G
Cuts at: 107 2460 107
Size: 2353 3169
NgoMIV G'CCGG_C
Cuts at: 210 813 3701 210
Size: 603 2888 2031
Fragments arranged by size:
2888 2031 603
NheI G'CTAG_C
Cuts at: 804 804
Size: 5522
NspI r_CATG'y
Cuts at: 760 1512 2575 3075 4678 760
Size: 752 1063 500 1603 1604
Fragments arranged by size:
1604 1603 1063 752 500
PasI CC'CwG_GG
Cuts at: 873 1029 873
Size: 156 5366
PciI A'CATG_T
Cuts at: 4674 4674
Size: 5522
PmeI GTTT'AAAC
Cuts at: 2521 2521
Size: 5522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 5490
PpuMI rG'GwC_Cy
Cuts at: 222 630 222
Size: 408 5114
PspOMI G'GGCC_C
Cuts at: 252 268 309 477 520 858 876 252
Size: 16 41 168 43 338 18 4898
Fragments arranged by size:
4898 338 168 43 41 18 16
PstI C_TGCA'G
Cuts at: 1085 1402 1727 2569 3254 1085
Size: 317 325 842 685 3353
Fragments arranged by size:
3353 842 685 325 317
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5522
PvuII CAG'CTG
Cuts at: 969 2947 3307 969
Size: 1978 360 3184
Fragments arranged by size:
3184 1978 360
RsrII CG'GwC_CG
Cuts at: 886 3717 886
Size: 2831 2691
SacI G_AGCT'C
Cuts at: 2032 2182 2536 2032
Size: 150 354 5018
Fragments arranged by size:
5018 354 150
SacII CC_GC'GG
Cuts at: 176 428 518 554 596 885 176
Size: 252 90 36 42 289 4813
Fragments arranged by size:
4813 289 252 90 42 36
SalI G'TCGA_C
Cuts at: 2559 2559
Size: 5522
SapI GCTCTTCn'nnn_
Cuts at: 3545 3755 3545
Size: 210 5312
SbfI CC_TGCA'GG
Cuts at: 1727 2569 1727
Size: 842 4680
ScaI AGT'ACT
Cuts at: 1959 5039 1959
Size: 3080 2442
SfcI C'TryA_G
Cuts at: 33 145 1081 1398 1723 2565 3250 4218 4409
Size: 112 936 317 325 842 685 968 191
Cuts at: 4409 33
Size: 1146
Fragments arranged by size:
1146 968 936 842 685 325 317 191
112
SfoI GGC'GCC
Cuts at: 332 839 2954 3202 332
Size: 507 2115 248 2652
Fragments arranged by size:
2652 2115 507 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
SgrAI Cr'CCGG_yG
Cuts at: 1453 1453
Size: 5522
SmaI CCC'GGG
Cuts at: 273 482 723 770 2544 273
Size: 209 241 47 1774 3251
Fragments arranged by size:
3251 1774 241 209 47
SmlI C'TyrA_G
Cuts at: 1069 1159 1521 2202 2677 3592 4306 4568 1069
Size: 90 362 681 475 915 714 262 2023
Fragments arranged by size:
2023 915 714 681 475 362 262 90
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5522
SphI G_CATG'C
Cuts at: 760 2575 3075 760
Size: 1815 500 3207
Fragments arranged by size:
3207 1815 500
SrfI GCCC'GGGC
Cuts at: 273 273
Size: 5522
StuI AGG'CCT
Cuts at: 1281 1281
Size: 5522
StyI C'CwwG_G
Cuts at: 107 2460 2656 3010 107
Size: 2353 196 354 2619
Fragments arranged by size:
2619 2353 354 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 355 397 1021 1890 2298 3105 3238 3697 3906
Size: 42 624 869 408 807 133 459 209
Cuts at: 3906 355
Size: 1971
Fragments arranged by size:
1971 869 807 624 459 408 209 133
42
TatI w'GTAC_w
Cuts at: 917 1151 1957 5037 917
Size: 234 806 3080 1402
Fragments arranged by size:
3080 1402 806 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5039 5071 5039
Size: 32 5490
Tth111I GACn'n_nGTC
Cuts at: 3319 3319
Size: 5522
XbaI T'CTAG_A
Cuts at: 2553 2553
Size: 5522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 686 3017 686
Size: 2331 3191
XmaI C'CCGG_G
Cuts at: 271 480 721 768 2542 271
Size: 209 241 47 1774 3251
Fragments arranged by size:
3251 1774 241 209 47
XmnI GAAnn'nnTTC
Cuts at: 1195 1588 1195
Size: 393 5129
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AloI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsgI BsiWI BsmI BsmBI BspEI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SnaBI SspI SwaI XhoI ZraI