Restriction map for
pF1KB2501
(Circular) (Six-base) MAPSORT of: pF1KB2501.seq Check: 3229 from: 1 to: 5027
pF1KB2501 5027 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2065 2065
Size: 5027
Acc65I G'GTAC_C
Cuts at: 2043 2043
Size: 5027
AclI AA'CG_TT
Cuts at: 4867 4867
Size: 5027
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 2852 3284 3637 4299 1331
Size: 1521 432 353 662 2059
Fragments arranged by size:
2059 1521 662 432 353
AflIII A'CryG_T
Cuts at: 491 1678 1828 1856 1858 2379 4179 4460 491
Size: 1187 150 28 2 521 1800 281 1058
Fragments arranged by size:
1800 1187 1058 521 281 150 28 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 510 510
Size: 5027
AlwNI CAG_nnn'CTG
Cuts at: 1516 2332 3770 1516
Size: 816 1438 2773
Fragments arranged by size:
2773 1438 816
ApaI G_GGCC'C
Cuts at: 709 759 709
Size: 50 4977
ApaLI G'TGCA_C
Cuts at: 3865 3865
Size: 5027
ApoI r'AATT_y
Cuts at: 994 1033 1178 1249 2031 4380 4636 994
Size: 39 145 71 782 2349 256 1385
Fragments arranged by size:
2349 1385 782 256 145 71 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 5027
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
AvaI C'yCGr_G
Cuts at: 322 429 700 2047 322
Size: 107 271 1347 3302
Fragments arranged by size:
3302 1347 271 107
BamHI G'GATC_C
Cuts at: 942 1169 2052 942
Size: 227 883 3917
Fragments arranged by size:
3917 883 227
BanI G'GyrC_C
Cuts at: 154 425 2043 2457 2705 2740 154
Size: 271 1618 414 248 35 2441
Fragments arranged by size:
2441 1618 414 271 248 35
BanII G_rGCy'C
Cuts at: 233 557 701 709 759 1332 1473 2041 3071
Size: 324 144 8 50 573 141 568 1030
Cuts at: 3071 233
Size: 2189
Fragments arranged by size:
2189 1030 573 568 324 144 141 50
8
BbeI G_GCGC'C
Cuts at: 2461 2709 2461
Size: 248 4779
BbsI GAAGACnn'nnnn_
Cuts at: 429 773 429
Size: 344 4683
BciVI GTATCCnnnnn_n'
Cuts at: 2925 3981 5027 2925
Size: 1056 1046 2925
Fragments arranged by size:
2925 1056 1046
BclI T'GATC_A
Cuts at: 2547 2547
Size: 5027
BglI GCCn_nnn'nGGC
Cuts at: 598 713 598
Size: 115 4912
BglII A'GATC_T
Cuts at: 2542 4579 2542
Size: 2037 2990
BlpI GC'TnA_GC
Cuts at: 2139 2139
Size: 5027
Bme1580I G_kGCm'C
Cuts at: 157 709 759 2652 2745 3869 157
Size: 552 50 1893 93 1124 1315
Fragments arranged by size:
1893 1315 1124 552 93 50
BmgBI CAC'GTC
Cuts at: 492 549 492
Size: 57 4970
BmrI ACTGGGnnnn_n'
Cuts at: 2239 2320 2655 4799 2239
Size: 81 335 2144 2467
Fragments arranged by size:
2467 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1967 2012 3394 1967
Size: 45 1382 3600
Fragments arranged by size:
3600 1382 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 750 1508 1937 750
Size: 758 429 3840
Fragments arranged by size:
3840 758 429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 2203 3082 3796 4094 169
Size: 2034 879 714 298 1102
Fragments arranged by size:
2034 1102 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 535 610 4731 535
Size: 75 4121 831
Fragments arranged by size:
4121 831 75
BsaAI yAC'GTr
Cuts at: 1859 2380 1859
Size: 521 4506
BsaBI GATnn'nnATC
Cuts at: 6 2541 4578 6
Size: 2535 2037 455
Fragments arranged by size:
2535 2037 455
BsaHI Gr'CG_yC
Cuts at: 434 590 2458 2706 3408 4904 434
Size: 156 1868 248 702 1496 557
Fragments arranged by size:
1868 1496 702 557 248 156
BsaWI w'CCGG_w
Cuts at: 2216 2737 3415 3826 3973 4247 2216
Size: 521 678 411 147 274 2996
Fragments arranged by size:
2996 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 959 989 959
Size: 30 4997
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 213 526 529 610 747 1598 213
Size: 313 3 81 137 851 3642
Fragments arranged by size:
3642 851 313 137 81 3
BseYI C'CCAG_C
Cuts at: 675 2452 3210 3875 4283 675
Size: 1777 758 665 408 1419
Fragments arranged by size:
1777 1419 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 460
Size: 5027
BsiEI CG_ry'CG
Cuts at: 104 405 423 2569 2615 3845 104
Size: 301 18 2146 46 1230 1286
Fragments arranged by size:
2146 1286 1230 301 46 18
BsiHKAI G_wGCw'C
Cuts at: 452 1332 1361 1482 2041 2819 3009 3869 452
Size: 880 29 121 559 778 190 860 1610
Fragments arranged by size:
1610 880 860 778 559 190 121 29
Bsp1286I G_dGCh'C
Cuts at: 157 233 452 557 701 709 759 1332 1361
Size: 76 219 105 144 8 50 573 29
Cuts at: 1361 1473 1482 2041 2652 2745 2819 3009 3071
Size: 112 9 559 611 93 74 190 62
Cuts at: 3071 3869 157
Size: 798 1315
Fragments arranged by size:
1315 798 611 573 559 219 190 144
112 105 93 76 74 62 50 29
9 8
BspHI T'CATG_A
Cuts at: 3459 3459
Size: 5027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2077 2291 2593 2974 2077
Size: 214 302 381 4130
Fragments arranged by size:
4130 381 302 214
BsrBI CCG'CTC
Cuts at: 50 504 3319 3373 4857 5022 50
Size: 454 2815 54 1484 165 55
Fragments arranged by size:
2815 1484 454 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1562 1941 2939 1562
Size: 379 998 3650
Fragments arranged by size:
3650 998 379
BsrFI r'CCGG_y
Cuts at: 422 466 575 3025 3206 422
Size: 44 109 2450 181 2243
Fragments arranged by size:
2450 2243 181 109 44
BssSI C'ACGA_G
Cuts at: 3298 4006 3298
Size: 708 4319
BstAPI GCAn_nnn'nTGC
Cuts at: 1936 2404 1936
Size: 468 4559
BstBI TT'CG_AA
Cuts at: 764 3388 764
Size: 2624 2403
BstXI CCAn_nnnn'nTGG
Cuts at: 397 675 397
Size: 278 4749
BstYI r'GATC_y
Cuts at: 1 942 1169 2052 2519 2542 2877 3123 3429
Size: 941 227 883 467 23 335 246 306
Cuts at: 3429 3441 3527 4579 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 941 883 467 449 335 306 246
227 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1147 1610 1147
Size: 463 4564
BtgI C'CryG_G
Cuts at: 107 251 369 107
Size: 144 118 4765
Fragments arranged by size:
4765 144 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2977 3158 3321 2977
Size: 181 163 4683
Fragments arranged by size:
4683 181 163
BtsI GCAGTG_nn'
Cuts at: 477 2270 477
Size: 1793 3234
DraI TTT'AAA
Cuts at: 2026 2026
Size: 5027
DraIII CAC_nnn'GTG
Cuts at: 1672 1672
Size: 5027
DrdI GACnn_nn'nnGTC
Cuts at: 2733 4077 2733
Size: 1344 3683
EaeI y'GGCC_r
Cuts at: 402 665 723 1070 2612 2786 3177 3204 4549
Size: 263 58 347 1542 174 391 27 1345
Cuts at: 4549 402
Size: 880
Fragments arranged by size:
1542 1345 880 391 347 263 174 58
27
EagI C'GGCC_G
Cuts at: 402 2612 402
Size: 2210 2817
EarI CTCTTCn'nnn_
Cuts at: 511 766 1235 1812 3050 3260 511
Size: 255 469 577 1238 210 2278
Fragments arranged by size:
2278 1238 577 469 255 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3235 3977 4123 4837 3235
Size: 742 146 714 3425
Fragments arranged by size:
3425 742 714 146
EcoICRI GAG'CTC
Cuts at: 1330 2039 1330
Size: 709 4318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 750 1331 1508 1937 2852 3284 3637 4299 750
Size: 581 177 429 915 432 353 662 1478
Fragments arranged by size:
1478 915 662 581 432 429 353 177
EcoO109I rG'GnC_Cy
Cuts at: 657 705 706 756 2166 657
Size: 48 1 50 1410 3518
Fragments arranged by size:
3518 1410 50 48 1
EcoRI G'AATT_C
Cuts at: 1033 2031 1033
Size: 998 4029
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1153 3046 1153
Size: 1893 3134
FspI TGC'GCA
Cuts at: 2808 2808
Size: 5027
HaeII r_GCGC'y
Cuts at: 310 604 1995 2461 2709 3939 4710 310
Size: 294 1391 466 248 1230 771 627
Fragments arranged by size:
1391 1230 771 627 466 294 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 703 735 760 792 1991 2023 2869 2899 2901
Size: 32 25 32 1199 32 846 30 2
Cuts at: 2901 2931 703
Size: 30 2799
Fragments arranged by size:
2799 1199 846 32 32 32 30 30
25 2
HincII GTy'rAC
Cuts at: 2066 2066
Size: 5027
HindIII A'AGCT_T
Cuts at: 863 1905 863
Size: 1042 3985
KasI G'GCGC_C
Cuts at: 2457 2705 2457
Size: 248 4779
KpnI G_GTAC'C
Cuts at: 2047 2047
Size: 5027
MluI A'CGCG_T
Cuts at: 4460 4460
Size: 5027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3786 3970 3786
Size: 184 4843
MscI TGG'CCA
Cuts at: 1072 2788 1072
Size: 1716 3311
MslI CAynn'nnrTG
Cuts at: 395 1462 1510 3016 3143 395
Size: 1067 48 1506 127 2279
Fragments arranged by size:
2279 1506 1067 127 48
MspA1I CmG'CkG
Cuts at: 315 371 728 2138 2452 2812 3596 3841 315
Size: 56 357 1410 314 360 784 245 1501
Fragments arranged by size:
1501 1410 784 360 357 314 245 56
NaeI GCC'GGC
Cuts at: 3208 3208
Size: 5027
NarI GG'CG_CC
Cuts at: 2458 2706 2458
Size: 248 4779
NcoI C'CATG_G
Cuts at: 107 107
Size: 5027
NgoMIV G'CCGG_C
Cuts at: 3206 3206
Size: 5027
NotI GC'GGCC_GC
Cuts at: 402 402
Size: 5027
NspI r_CATG'y
Cuts at: 753 1461 1682 1832 2080 2580 4183 753
Size: 708 221 150 248 500 1603 1597
Fragments arranged by size:
1603 1597 708 500 248 221 150
PasI CC'CwG_GG
Cuts at: 1419 1419
Size: 5027
PciI A'CATG_T
Cuts at: 1678 1828 4179 1678
Size: 150 2351 2526
Fragments arranged by size:
2526 2351 150
PmeI GTTT'AAAC
Cuts at: 2026 2026
Size: 5027
PmlI CAC'GTG
Cuts at: 1859 1859
Size: 5027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4995
PpuMI rG'GwC_Cy
Cuts at: 657 657
Size: 5027
PshAI GACnn'nnGTC
Cuts at: 1679 1679
Size: 5027
PspOMI G'GGCC_C
Cuts at: 705 755 705
Size: 50 4977
PstI C_TGCA'G
Cuts at: 272 364 743 2074 2759 272
Size: 92 379 1331 685 2540
Fragments arranged by size:
2540 1331 685 379 92
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5027
PvuII CAG'CTG
Cuts at: 2452 2812 2452
Size: 360 4667
RsrII CG'GwC_CG
Cuts at: 3222 3222
Size: 5027
SacI G_AGCT'C
Cuts at: 1332 2041 1332
Size: 709 4318
SacII CC_GC'GG
Cuts at: 372 372
Size: 5027
SalI G'TCGA_C
Cuts at: 2064 2064
Size: 5027
SapI GCTCTTCn'nnn_
Cuts at: 511 3050 3260 511
Size: 2539 210 2278
Fragments arranged by size:
2539 2278 210
SbfI CC_TGCA'GG
Cuts at: 2074 2074
Size: 5027
ScaI AGT'ACT
Cuts at: 1242 4544 1242
Size: 3302 1725
SfcI C'TryA_G
Cuts at: 33 268 360 739 922 1400 2070 2755 3723
Size: 235 92 379 183 478 670 685 968
Cuts at: 3723 3914 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 670 478 379 235 191
183 92
SfoI GGC'GCC
Cuts at: 2459 2707 2459
Size: 248 4779
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
SmaI CCC'GGG
Cuts at: 324 431 2049 324
Size: 107 1618 3302
Fragments arranged by size:
3302 1618 107
SmlI C'TyrA_G
Cuts at: 184 2182 3097 3811 4073 184
Size: 1998 915 714 262 1138
Fragments arranged by size:
1998 1138 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5027
SphI G_CATG'C
Cuts at: 1461 2080 2580 1461
Size: 619 500 3908
Fragments arranged by size:
3908 619 500
SspI AAT'ATT
Cuts at: 873 873
Size: 5027
StyI C'CwwG_G
Cuts at: 107 1550 1576 2161 2515 107
Size: 1443 26 585 354 2619
Fragments arranged by size:
2619 1443 585 354 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 559 2610 2743 3202 3411 559
Size: 2051 133 459 209 2175
Fragments arranged by size:
2175 2051 459 209 133
TatI w'GTAC_w
Cuts at: 1240 1541 1663 1741 4542 1240
Size: 301 122 78 2801 1725
Fragments arranged by size:
2801 1725 301 122 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 959 991 4544 4576 959
Size: 32 3553 32 1410
Fragments arranged by size:
3553 1410 32 32
Tth111I GACn'n_nGTC
Cuts at: 2824 2824
Size: 5027
XbaI T'CTAG_A
Cuts at: 2058 2058
Size: 5027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1416 2522 1416
Size: 1106 3921
XmaI C'CCGG_G
Cuts at: 322 429 2047 322
Size: 107 1618 3302
Fragments arranged by size:
3302 1618 107
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstZ17I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NruI NsiI PacI PflMI PfoI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI