Restriction map for
pF1KB2507
(Circular) (Six-base) MAPSORT of: pF1KB2507.seq Check: 2126 from: 1 to: 4295
pF1KB2507 4295 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1333 1333
Size: 4295
Acc65I G'GTAC_C
Cuts at: 1311 1311
Size: 4295
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2120 2552 2905 3567 2120
Size: 432 353 662 2848
Fragments arranged by size:
2848 662 432 353
AflIII A'CryG_T
Cuts at: 1647 3447 3728 1647
Size: 1800 281 2214
Fragments arranged by size:
2214 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 669 701 669
Size: 32 4263
AlwNI CAG_nnn'CTG
Cuts at: 1600 3038 1600
Size: 1438 2857
ApaLI G'TGCA_C
Cuts at: 3133 3133
Size: 4295
ApoI r'AATT_y
Cuts at: 1299 3648 3904 1299
Size: 2349 256 1690
Fragments arranged by size:
2349 1690 256
AseI AT'TA_AT
Cuts at: 20 470 482 20
Size: 450 12 3833
Fragments arranged by size:
3833 450 12
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 1315 1315
Size: 4295
BamHI G'GATC_C
Cuts at: 1320 1320
Size: 4295
BanI G'GyrC_C
Cuts at: 1311 1725 1973 2008 1311
Size: 414 248 35 3598
Fragments arranged by size:
3598 414 248 35
BanII G_rGCy'C
Cuts at: 1309 2339 1309
Size: 1030 3265
BbeI G_GCGC'C
Cuts at: 1729 1977 1729
Size: 248 4047
BbsI GAAGACnn'nnnn_
Cuts at: 138 138
Size: 4295
BciVI GTATCCnnnnn_n'
Cuts at: 2193 3249 4295 2193
Size: 1056 1046 2193
Fragments arranged by size:
2193 1056 1046
BclI T'GATC_A
Cuts at: 926 1815 926
Size: 889 3406
BfrBI ATG'CAT
Cuts at: 183 183
Size: 4295
BglII A'GATC_T
Cuts at: 1810 3847 1810
Size: 2037 2258
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 1920 2013 3137 1920
Size: 93 1124 3078
Fragments arranged by size:
3078 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1507 1588 1923 4067 1507
Size: 81 335 2144 1735
Fragments arranged by size:
2144 1735 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1251 1251
Size: 4295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 1471 2350 3064 3362 854
Size: 617 879 714 298 1787
Fragments arranged by size:
1787 879 714 617 298
BsaI GGTCTCn'nnnn_
Cuts at: 3999 3999
Size: 4295
BsaAI yAC'GTr
Cuts at: 627 1648 627
Size: 1021 3274
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 1726 1974 2676 4172 1726
Size: 248 702 1496 1849
Fragments arranged by size:
1849 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1484 2005 2683 3094 3241 3515 1484
Size: 521 678 411 147 274 2264
Fragments arranged by size:
2264 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1224 1254 1224
Size: 30 4265
BseYI C'CCAG_C
Cuts at: 1720 2478 3143 3551 1720
Size: 758 665 408 2464
Fragments arranged by size:
2464 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3113 104
Size: 1733 46 1230 1286
Fragments arranged by size:
1733 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 359 1309 2087 2277 3137 359
Size: 950 778 190 860 1517
Fragments arranged by size:
1517 950 860 778 190
BsmI GAATG_Cn'
Cuts at: 997 997
Size: 4295
Bsp1286I G_dGCh'C
Cuts at: 359 1309 1920 2013 2087 2277 2339 3137 359
Size: 950 611 93 74 190 62 798 1517
Fragments arranged by size:
1517 950 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 375 2727 375
Size: 2352 1943
BspMI ACCTGCnnnn'nnnn_
Cuts at: 654 1345 1559 1861 2242 654
Size: 691 214 302 381 2707
Fragments arranged by size:
2707 691 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2587 2641 4125 4290 50
Size: 2537 54 1484 165 55
Fragments arranged by size:
2537 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 803 866 884 1071 2207 803
Size: 63 18 187 1136 2891
Fragments arranged by size:
2891 1136 187 63 18
BsrFI r'CCGG_y
Cuts at: 2293 2474 2293
Size: 181 4114
BsrGI T'GTAC_A
Cuts at: 366 366
Size: 4295
BssSI C'ACGA_G
Cuts at: 2566 3274 2566
Size: 708 3587
BstAPI GCAn_nnn'nTGC
Cuts at: 1672 1672
Size: 4295
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstYI r'GATC_y
Cuts at: 1 294 1320 1787 1810 2145 2391 2697 2709
Size: 293 1026 467 23 335 246 306 12
Cuts at: 2709 2795 3847 1
Size: 86 1052 449
Fragments arranged by size:
1052 1026 467 449 335 306 293 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2245 2426 2589 2245
Size: 181 163 3951
Fragments arranged by size:
3951 181 163
BtsI GCAGTG_nn'
Cuts at: 1538 1538
Size: 4295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 855 890 855
Size: 35 4260
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DraIII CAC_nnn'GTG
Cuts at: 1035 1133 1035
Size: 98 4197
DrdI GACnn_nn'nnGTC
Cuts at: 738 2001 3345 738
Size: 1263 1344 1688
Fragments arranged by size:
1688 1344 1263
EaeI y'GGCC_r
Cuts at: 140 824 1880 2054 2445 2472 3817 140
Size: 684 1056 174 391 27 1345 618
Fragments arranged by size:
1345 1056 684 618 391 174 27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 166 275 2318 2528 166
Size: 109 2043 210 1933
Fragments arranged by size:
2043 1933 210 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2503 3245 3391 4105 2503
Size: 742 146 714 2693
Fragments arranged by size:
2693 742 714 146
EcoICRI GAG'CTC
Cuts at: 1307 1307
Size: 4295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1251 2120 2552 2905 3567 1251
Size: 869 432 353 662 1979
Fragments arranged by size:
1979 869 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1215 1434 1215
Size: 219 4076
EcoRI G'AATT_C
Cuts at: 1299 1299
Size: 4295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 376 2314 376
Size: 1938 2357
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 115 1729 1977 3207 3978 115
Size: 1614 248 1230 771 432
Fragments arranged by size:
1614 1230 771 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 200 232 683 715 1224 1256 2137 2167 2169
Size: 32 451 32 509 32 881 30 2
Cuts at: 2169 2199 200
Size: 30 2296
Fragments arranged by size:
2296 881 509 451 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1334 1334
Size: 4295
HindIII A'AGCT_T
Cuts at: 406 406
Size: 4295
KasI G'GCGC_C
Cuts at: 1725 1973 1725
Size: 248 4047
KpnI G_GTAC'C
Cuts at: 1315 1315
Size: 4295
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 746 829 3054 3238 746
Size: 83 2225 184 1803
Fragments arranged by size:
2225 1803 184 83
MscI TGG'CCA
Cuts at: 826 2056 826
Size: 1230 3065
MslI CAynn'nnrTG
Cuts at: 2284 2411 2284
Size: 127 4168
MspA1I CmG'CkG
Cuts at: 1406 1720 2080 2864 3109 1406
Size: 314 360 784 245 2592
Fragments arranged by size:
2592 784 360 314 245
NaeI GCC'GGC
Cuts at: 2476 2476
Size: 4295
NarI GG'CG_CC
Cuts at: 1726 1974 1726
Size: 248 4047
NcoI C'CATG_G
Cuts at: 107 107
Size: 4295
NgoMIV G'CCGG_C
Cuts at: 2474 2474
Size: 4295
NsiI A_TGCA'T
Cuts at: 185 185
Size: 4295
NspI r_CATG'y
Cuts at: 957 1348 1848 3451 957
Size: 391 500 1603 1801
Fragments arranged by size:
1801 1603 500 391
PciI A'CATG_T
Cuts at: 3447 3447
Size: 4295
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 4263
PpuMI rG'GwC_Cy
Cuts at: 1215 1215
Size: 4295
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 593 625 593
Size: 32 4263
PstI C_TGCA'G
Cuts at: 1342 2027 1342
Size: 685 3610
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 1720 2080 1720
Size: 360 3935
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1309 1309
Size: 4295
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SanDI GG'GwC_CC
Cuts at: 1215 1215
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 3812 3812
Size: 4295
SfcI C'TryA_G
Cuts at: 33 1245 1338 2023 2991 3182 33
Size: 1212 93 685 968 191 1146
Fragments arranged by size:
1212 1146 968 685 191 93
SfoI GGC'GCC
Cuts at: 1727 1975 1727
Size: 248 4047
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 869 1450 2365 3079 3341 869
Size: 581 915 714 262 1823
Fragments arranged by size:
1823 915 714 581 262
SnaBI TAC'GTA
Cuts at: 627 627
Size: 4295
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
StyI C'CwwG_G
Cuts at: 107 128 1429 1783 107
Size: 21 1301 354 2619
Fragments arranged by size:
2619 1301 354 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 857 1878 2011 2470 2679 857
Size: 1021 133 459 209 2473
Fragments arranged by size:
2473 1021 459 209 133
TatI w'GTAC_w
Cuts at: 366 800 3810 366
Size: 434 3010 851
Fragments arranged by size:
3010 851 434
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4263
Tth111I GACn'n_nGTC
Cuts at: 2092 2092
Size: 4295
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1226 1790 114
Size: 1112 564 2619
Fragments arranged by size:
2619 1112 564
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmBI BspEI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
SacII SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI