Restriction map for
pF1KB3002
(Circular) (Six-base) MAPSORT of: pF1KB3002.seq Check: 5363 from: 1 to: 4838
pF1KB3002 4838 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1391 1876 1391
Size: 485 4353
Acc65I G'GTAC_C
Cuts at: 1163 1854 1163
Size: 691 4147
AclI AA'CG_TT
Cuts at: 4678 4678
Size: 4838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 577 1258 1422 1770 2663 3095 3448 4110
Size: 129 681 164 348 893 432 353 662
Cuts at: 4110 448
Size: 1176
Fragments arranged by size:
1176 893 681 662 432 353 348 164
129
AflIII A'CryG_T
Cuts at: 2190 3990 4271 2190
Size: 1800 281 2757
Fragments arranged by size:
2757 1800 281
AleI CACnn'nnGTG
Cuts at: 1711 1711
Size: 4838
AlwNI CAG_nnn'CTG
Cuts at: 805 2143 3581 805
Size: 1338 1438 2062
Fragments arranged by size:
2062 1438 1338
ApaI G_GGCC'C
Cuts at: 246 340 936 246
Size: 94 596 4148
Fragments arranged by size:
4148 596 94
ApaLI G'TGCA_C
Cuts at: 830 3676 830
Size: 2846 1992
ApoI r'AATT_y
Cuts at: 152 1842 4191 4447 152
Size: 1690 2349 256 543
Fragments arranged by size:
2349 1690 543 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4838
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
AvaI C'yCGr_G
Cuts at: 275 420 1858 275
Size: 145 1438 3255
Fragments arranged by size:
3255 1438 145
AvrII C'CTAG_G
Cuts at: 193 193
Size: 4838
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1416 1449 1416
Size: 33 4805
BamHI G'GATC_C
Cuts at: 111 1863 111
Size: 1752 3086
BanI G'GyrC_C
Cuts at: 574 660 1163 1562 1568 1587 1854 2268 2516
Size: 86 503 399 6 19 267 414 248
Cuts at: 2516 2551 574
Size: 35 2861
Fragments arranged by size:
2861 503 414 399 267 248 86 35
19 6
BanII G_rGCy'C
Cuts at: 235 246 276 340 390 936 1118 1662 1852
Size: 11 30 64 50 546 182 544 190
Cuts at: 1852 2882 235
Size: 1030 2191
Fragments arranged by size:
2191 1030 546 544 190 182 64 50
30 11
BbeI G_GCGC'C
Cuts at: 1572 2272 2520 1572
Size: 700 248 3890
Fragments arranged by size:
3890 700 248
BbsI GAAGACnn'nnnn_
Cuts at: 1196 1450 1776 1196
Size: 254 326 4258
Fragments arranged by size:
4258 326 254
BbvCI CC'TCA_GC
Cuts at: 1702 1702
Size: 4838
BciVI GTATCCnnnnn_n'
Cuts at: 1021 2736 3792 4838 1021
Size: 1715 1056 1046 1021
Fragments arranged by size:
1715 1056 1046 1021
BclI T'GATC_A
Cuts at: 2358 2358
Size: 4838
BfrBI ATG'CAT
Cuts at: 166 166
Size: 4838
BglI GCCn_nnn'nGGC
Cuts at: 1557 1557
Size: 4838
BglII A'GATC_T
Cuts at: 2353 4390 2353
Size: 2037 2801
BlpI GC'TnA_GC
Cuts at: 229 428 451 1820 1950 229
Size: 199 23 1369 130 3117
Fragments arranged by size:
3117 1369 199 130 23
Bme1580I G_kGCm'C
Cuts at: 246 340 834 936 1592 1614 2463 2556 3680
Size: 94 494 102 656 22 849 93 1124
Cuts at: 3680 246
Size: 1404
Fragments arranged by size:
1404 1124 849 656 494 102 94 93
22
BmgBI CAC'GTC
Cuts at: 1395 1395
Size: 4838
BmrI ACTGGGnnnn_n'
Cuts at: 506 1839 2050 2131 2466 4610 506
Size: 1333 211 81 335 2144 734
Fragments arranged by size:
2144 1333 734 335 211 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1137 3205 1137
Size: 2068 2770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1078
Size: 4838
Bpu10I CC'TnA_GC
Cuts at: 1702 1702
Size: 4838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 825 949 2014 2893 3607 3905 796
Size: 29 124 1065 879 714 298 1729
Fragments arranged by size:
1729 1065 879 714 298 124 29
BsaI GGTCTCn'nnnn_
Cuts at: 4542 4542
Size: 4838
BsaAI yAC'GTr
Cuts at: 2191 2191
Size: 4838
BsaBI GATnn'nnATC
Cuts at: 6 2352 4389 6
Size: 2346 2037 455
Fragments arranged by size:
2346 2037 455
BsaHI Gr'CG_yC
Cuts at: 1569 2269 2517 3219 4715 1569
Size: 700 248 702 1496 1692
Fragments arranged by size:
1692 1496 702 700 248
BsaWI w'CCGG_w
Cuts at: 1718 2027 2548 3226 3637 3784 4058 1718
Size: 309 521 678 411 147 274 2498
Fragments arranged by size:
2498 678 521 411 309 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 754 784 754
Size: 30 4808
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 486 599 602 605 608 629 1291 1516 486
Size: 113 3 3 3 21 662 225 3808
Fragments arranged by size:
3808 662 225 113 21 3 3 3
BseYI C'CCAG_C
Cuts at: 410 538 677 798 1138 1220 2263 3021 3686
Size: 128 139 121 340 82 1043 758 665
Cuts at: 3686 4094 410
Size: 408 1154
Fragments arranged by size:
1154 1043 758 665 408 340 139 128
121 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 1619 749
Size: 870 3968
BsiEI CG_ry'CG
Cuts at: 104 2380 2426 3656 104
Size: 2276 46 1230 1286
Fragments arranged by size:
2276 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 235 429 834 1118 1400 1852 2630 2820 3680
Size: 194 405 284 282 452 778 190 860
Cuts at: 3680 235
Size: 1393
Fragments arranged by size:
1393 860 778 452 405 284 282 194
190
Bsp1286I G_dGCh'C
Cuts at: 235 246 276 340 390 429 834 936 1118
Size: 11 30 64 50 39 405 102 182
Cuts at: 1118 1400 1592 1614 1662 1852 2463 2556 2630
Size: 282 192 22 48 190 611 93 74
Cuts at: 2630 2820 2882 3680 235
Size: 190 62 798 1393
Fragments arranged by size:
1393 798 611 405 282 192 190 190
182 102 93 74 64 62 50 48
39 30 22 11
BspEI T'CCGG_A
Cuts at: 1718 1718
Size: 4838
BspHI T'CATG_A
Cuts at: 3270 3270
Size: 4838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1824 1888 2102 2404 2785 1824
Size: 64 214 302 381 3877
Fragments arranged by size:
3877 381 302 214 64
BsrBI CCG'CTC
Cuts at: 50 1064 1649 1824 3130 3184 4668 4833 50
Size: 1014 585 175 1306 54 1484 165 55
Fragments arranged by size:
1484 1306 1014 585 175 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2750 2750
Size: 4838
BsrFI r'CCGG_y
Cuts at: 1418 1538 1565 1627 2836 3017 1418
Size: 120 27 62 1209 181 3239
Fragments arranged by size:
3239 1209 181 120 62 27
BssSI C'ACGA_G
Cuts at: 3109 3817 3109
Size: 708 4130
BstAPI GCAn_nnn'nTGC
Cuts at: 2215 2215
Size: 4838
BstBI TT'CG_AA
Cuts at: 3199 3199
Size: 4838
BstYI r'GATC_y
Cuts at: 1 111 1052 1863 2330 2353 2688 2934 3240
Size: 110 941 811 467 23 335 246 306
Cuts at: 3240 3252 3338 4390 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 941 811 467 449 335 306 246
110 86 23 12
BtgI C'CryG_G
Cuts at: 107 1728 107
Size: 1621 3217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2788 2969 3132 2788
Size: 181 163 4494
Fragments arranged by size:
4494 181 163
BtsI GCAGTG_nn'
Cuts at: 1706 2081 1706
Size: 375 4463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 756 791 756
Size: 35 4803
DraI TTT'AAA
Cuts at: 1837 1837
Size: 4838
DraIII CAC_nnn'GTG
Cuts at: 357 357
Size: 4838
DrdI GACnn_nn'nnGTC
Cuts at: 2544 3888 2544
Size: 1344 3494
EaeI y'GGCC_r
Cuts at: 315 756 896 1416 1664 1749 2423 2597 2988
Size: 441 140 520 248 85 674 174 391
Cuts at: 2988 3015 4360 315
Size: 27 1345 793
Fragments arranged by size:
1345 793 674 520 441 391 248 174
140 85 27
EagI C'GGCC_G
Cuts at: 2423 2423
Size: 4838
EarI CTCTTCn'nnn_
Cuts at: 197 215 1123 2861 3071 197
Size: 18 908 1738 210 1964
Fragments arranged by size:
1964 1738 908 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 382 3046 3788 3934 4648 382
Size: 2664 742 146 714 572
Fragments arranged by size:
2664 742 714 572 146
EcoICRI GAG'CTC
Cuts at: 233 1116 1850 233
Size: 883 734 3221
Fragments arranged by size:
3221 883 734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 577 1078 1258 1422 1770 2663 3095 3448
Size: 129 501 180 164 348 893 432 353
Cuts at: 3448 4110 448
Size: 662 1176
Fragments arranged by size:
1176 893 662 501 432 353 348 180
164 129
EcoO109I rG'GnC_Cy
Cuts at: 336 585 932 933 1744 1977 336
Size: 249 347 1 811 233 3197
Fragments arranged by size:
3197 811 347 249 233 1
EcoRI G'AATT_C
Cuts at: 1842 1842
Size: 4838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1800 2857 1800
Size: 1057 3781
FspI TGC'GCA
Cuts at: 256 2619 256
Size: 2363 2475
FspAI rTGC'GCAy
Cuts at: 256 256
Size: 4838
HaeII r_GCGC'y
Cuts at: 1572 2272 2520 3750 4521 1572
Size: 700 248 1230 771 1889
Fragments arranged by size:
1889 1230 771 700 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 485 517 1405 1437 2680 2710 2712
Size: 32 299 32 888 32 1243 30 2
Cuts at: 2712 2742 154
Size: 30 2250
Fragments arranged by size:
2250 1243 888 299 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1392 1877 1392
Size: 485 4353
KasI G'GCGC_C
Cuts at: 1568 2268 2516 1568
Size: 700 248 3890
Fragments arranged by size:
3890 700 248
KpnI G_GTAC'C
Cuts at: 1167 1858 1167
Size: 691 4147
MluI A'CGCG_T
Cuts at: 4271 4271
Size: 4838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1148 3597 3781 1148
Size: 2449 184 2205
Fragments arranged by size:
2449 2205 184
MscI TGG'CCA
Cuts at: 758 898 1666 2599 758
Size: 140 768 933 2997
Fragments arranged by size:
2997 933 768 140
MslI CAynn'nnrTG
Cuts at: 161 358 1091 1247 1412 1582 1585 1711 2827
Size: 197 733 156 165 170 3 126 1116
Cuts at: 2827 2954 161
Size: 127 2045
Fragments arranged by size:
2045 1116 733 197 170 165 156 127
126 3
MspA1I CmG'CkG
Cuts at: 414 432 733 1949 2263 2623 3407 3652 414
Size: 18 301 1216 314 360 784 245 1600
Fragments arranged by size:
1600 1216 784 360 314 301 245 18
NaeI GCC'GGC
Cuts at: 1540 3019 1540
Size: 1479 3359
NarI GG'CG_CC
Cuts at: 1569 2269 2517 1569
Size: 700 248 3890
Fragments arranged by size:
3890 700 248
NcoI C'CATG_G
Cuts at: 107 1728 107
Size: 1621 3217
NgoMIV G'CCGG_C
Cuts at: 1538 3017 1538
Size: 1479 3359
NsiI A_TGCA'T
Cuts at: 168 168
Size: 4838
NspI r_CATG'y
Cuts at: 1352 1699 1891 2391 3994 1352
Size: 347 192 500 1603 2196
Fragments arranged by size:
2196 1603 500 347 192
PasI CC'CwG_GG
Cuts at: 116 333 936 1015 1271 1546 116
Size: 217 603 79 256 275 3408
Fragments arranged by size:
3408 603 275 256 217 79
PciI A'CATG_T
Cuts at: 3990 3990
Size: 4838
PflMI CCAn_nnn'nTGG
Cuts at: 1130 1130
Size: 4838
PfoI T'CCnGG_A
Cuts at: 812 1055 1256 812
Size: 243 201 4394
Fragments arranged by size:
4394 243 201
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1685 1717 3284 3316 1685
Size: 32 1567 32 3207
Fragments arranged by size:
3207 1567 32 32
PspOMI G'GGCC_C
Cuts at: 242 336 932 242
Size: 94 596 4148
Fragments arranged by size:
4148 596 94
PstI C_TGCA'G
Cuts at: 1009 1885 2570 1009
Size: 876 685 3277
Fragments arranged by size:
3277 876 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4838
PvuII CAG'CTG
Cuts at: 414 432 733 2263 2623 414
Size: 18 301 1530 360 2629
Fragments arranged by size:
2629 1530 360 301 18
RsrII CG'GwC_CG
Cuts at: 3033 3033
Size: 4838
SacI G_AGCT'C
Cuts at: 235 1118 1852 235
Size: 883 734 3221
Fragments arranged by size:
3221 883 734
SalI G'TCGA_C
Cuts at: 1390 1875 1390
Size: 485 4353
SapI GCTCTTCn'nnn_
Cuts at: 1123 2861 3071 1123
Size: 1738 210 2890
Fragments arranged by size:
2890 1738 210
SbfI CC_TGCA'GG
Cuts at: 1885 1885
Size: 4838
ScaI AGT'ACT
Cuts at: 179 1525 4355 179
Size: 1346 2830 662
Fragments arranged by size:
2830 1346 662
SfcI C'TryA_G
Cuts at: 33 906 1005 1283 1593 1881 2566 3534 3725
Size: 873 99 278 310 288 685 968 191
Cuts at: 3725 33
Size: 1146
Fragments arranged by size:
1146 968 873 685 310 288 278 191
99
SfoI GGC'GCC
Cuts at: 1570 2270 2518 1570
Size: 700 248 3890
Fragments arranged by size:
3890 700 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
SgrAI Cr'CCGG_yG
Cuts at: 1565 1565
Size: 4838
SmaI CCC'GGG
Cuts at: 277 422 1860 277
Size: 145 1438 3255
Fragments arranged by size:
3255 1438 145
SmlI C'TyrA_G
Cuts at: 775 840 964 1993 2908 3622 3884 775
Size: 65 124 1029 915 714 262 1729
Fragments arranged by size:
1729 1029 915 714 262 124 65
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4838
SphI G_CATG'C
Cuts at: 1891 2391 1891
Size: 500 4338
SrfI GCCC'GGGC
Cuts at: 277 277
Size: 4838
StuI AGG'CCT
Cuts at: 630 1003 1207 1321 1799 630
Size: 373 204 114 478 3669
Fragments arranged by size:
3669 478 373 204 114
StyI C'CwwG_G
Cuts at: 107 193 947 1673 1728 1972 2326 107
Size: 86 754 726 55 244 354 2619
Fragments arranged by size:
2619 754 726 354 244 86 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 592 685 1424 1741 2421 2554 3013 3222 592
Size: 93 739 317 680 133 459 209 2208
Fragments arranged by size:
2208 739 680 459 317 209 133 93
TatI w'GTAC_w
Cuts at: 177 1523 4353 177
Size: 1346 2830 662
Fragments arranged by size:
2830 1346 662
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4355 4387 4355
Size: 32 4806
Tth111I GACn'n_nGTC
Cuts at: 2635 2635
Size: 4838
XbaI T'CTAG_A
Cuts at: 1869 1869
Size: 4838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 519 1504 1659 2333 519
Size: 985 155 674 3024
Fragments arranged by size:
3024 985 674 155
XmaI C'CCGG_G
Cuts at: 275 420 1858 275
Size: 145 1438 3255
Fragments arranged by size:
3255 1438 145
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI BcgI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SspI SwaI XhoI XmnI ZraI