Restriction map for
pF1KB3004
(Circular) (Six-base) MAPSORT of: pF1KB3004.seq Check: 7858 from: 1 to: 6263
pF1KB3004 6263 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2960 3301 2960
Size: 341 5922
Acc65I G'GTAC_C
Cuts at: 3279 3279
Size: 6263
AclI AA'CG_TT
Cuts at: 406 2547 6103 406
Size: 2141 3556 566
Fragments arranged by size:
3556 2141 566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 256 1379 1878 2231 2320 2623 4088 4520
Size: 42 1123 499 353 89 303 1465 432
Cuts at: 4520 4873 5535 214
Size: 353 662 942
Fragments arranged by size:
1465 1123 942 662 499 432 353 353
303 89 42
AflIII A'CryG_T
Cuts at: 3615 5415 5696 3615
Size: 1800 281 4182
Fragments arranged by size:
4182 1800 281
AleI CACnn'nnGTG
Cuts at: 2271 2271
Size: 6263
AlwNI CAG_nnn'CTG
Cuts at: 2298 2913 3136 3568 5006 2298
Size: 615 223 432 1438 3555
Fragments arranged by size:
3555 1438 615 432 223
ApaLI G'TGCA_C
Cuts at: 5101 5101
Size: 6263
ApoI r'AATT_y
Cuts at: 302 1333 1349 1846 2213 3091 3267 5616 5872
Size: 1031 16 497 367 878 176 2349 256
Cuts at: 5872 302
Size: 693
Fragments arranged by size:
2349 1031 878 693 497 367 256 176
16
AseI AT'TA_AT
Cuts at: 20 20
Size: 6263
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6263
AvaI C'yCGr_G
Cuts at: 701 3283 701
Size: 2582 3681
BamHI G'GATC_C
Cuts at: 3288 3288
Size: 6263
BanI G'GyrC_C
Cuts at: 2457 2612 3279 3693 3941 3976 2457
Size: 155 667 414 248 35 4744
Fragments arranged by size:
4744 667 414 248 155 35
BanII G_rGCy'C
Cuts at: 828 2119 3277 4307 828
Size: 1291 1158 1030 2784
Fragments arranged by size:
2784 1291 1158 1030
BbeI G_GCGC'C
Cuts at: 2461 3697 3945 2461
Size: 1236 248 4779
Fragments arranged by size:
4779 1236 248
BbsI GAAGACnn'nnnn_
Cuts at: 755 1779 1931 2135 2200 2351 3012 755
Size: 1024 152 204 65 151 661 4006
Fragments arranged by size:
4006 1024 661 204 152 151 65
BbvCI CC'TCA_GC
Cuts at: 2887 2887
Size: 6263
BciVI GTATCCnnnnn_n'
Cuts at: 1971 4161 5217 6263 1971
Size: 2190 1056 1046 1971
Fragments arranged by size:
2190 1971 1056 1046
BclI T'GATC_A
Cuts at: 1503 2565 3783 1503
Size: 1062 1218 3983
Fragments arranged by size:
3983 1218 1062
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 6263
BglII A'GATC_T
Cuts at: 2034 3778 5815 2034
Size: 1744 2037 2482
Fragments arranged by size:
2482 2037 1744
BlpI GC'TnA_GC
Cuts at: 2113 3375 2113
Size: 1262 5001
Bme1580I G_kGCm'C
Cuts at: 3888 3981 5105 3888
Size: 93 1124 5046
Fragments arranged by size:
5046 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1115 1673 3475 3556 3891 6035 1115
Size: 558 1802 81 335 2144 1343
Fragments arranged by size:
2144 1802 1343 558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 868 4630 868
Size: 3762 2501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1814 178
Size: 1636 4627
Bpu10I CC'TnA_GC
Cuts at: 1204 2887 1204
Size: 1683 4580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 751 838 1887 2518 2775 3439 4318 5032
Size: 389 87 1049 631 257 664 879 714
Cuts at: 5032 5330 362
Size: 298 1295
Fragments arranged by size:
1295 1049 879 714 664 631 389 298
257 87
BsaI GGTCTCn'nnnn_
Cuts at: 5967 5967
Size: 6263
BsaAI yAC'GTr
Cuts at: 3616 3616
Size: 6263
BsaBI GATnn'nnATC
Cuts at: 6 2564 3777 5814 6
Size: 2558 1213 2037 455
Fragments arranged by size:
2558 2037 1213 455
BsaHI Gr'CG_yC
Cuts at: 2458 3694 3942 4644 6140 2458
Size: 1236 248 702 1496 2581
Fragments arranged by size:
2581 1496 1236 702 248
BsaWI w'CCGG_w
Cuts at: 1367 2025 3452 3973 4651 5062 5209 5483 1367
Size: 658 1427 521 678 411 147 274 2147
Fragments arranged by size:
2147 1427 678 658 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 760 1291 2142 2184 760
Size: 531 851 42 4839
Fragments arranged by size:
4839 851 531 42
BseYI C'CCAG_C
Cuts at: 680 3688 4446 5111 5519 680
Size: 3008 758 665 408 1424
Fragments arranged by size:
3008 1424 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 1433 2887 1133
Size: 300 1454 4509
Fragments arranged by size:
4509 1454 300
BsiEI CG_ry'CG
Cuts at: 104 3805 3851 5081 104
Size: 3701 46 1230 1286
Fragments arranged by size:
3701 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 138 240 828 852 3277 4055 4245 5105 138
Size: 102 588 24 2425 778 190 860 1296
Fragments arranged by size:
2425 1296 860 778 588 190 102 24
BsmI GAATG_Cn'
Cuts at: 3117 3117
Size: 6263
BsmBI CGTCTCn'nnnn_
Cuts at: 1510 1510
Size: 6263
Bsp1286I G_dGCh'C
Cuts at: 138 240 828 852 2119 3277 3888 3981 4055
Size: 102 588 24 1267 1158 611 93 74
Cuts at: 4055 4245 4307 5105 138
Size: 190 62 798 1296
Fragments arranged by size:
1296 1267 1158 798 611 588 190 102
93 74 62 24
BspEI T'CCGG_A
Cuts at: 1367 1367
Size: 6263
BspHI T'CATG_A
Cuts at: 1974 4695 1974
Size: 2721 3542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1046 3313 3527 3829 4210 1046
Size: 2267 214 302 381 3099
Fragments arranged by size:
3099 2267 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4555 4609 6093 6258 50
Size: 4505 54 1484 165 55
Fragments arranged by size:
4505 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 510 4175 510
Size: 3665 2598
BsrFI r'CCGG_y
Cuts at: 4261 4442 4261
Size: 181 6082
BsrGI T'GTAC_A
Cuts at: 192 521 2904 192
Size: 329 2383 3551
Fragments arranged by size:
3551 2383 329
BssSI C'ACGA_G
Cuts at: 1076 1754 4534 5242 1076
Size: 678 2780 708 2097
Fragments arranged by size:
2780 2097 708 678
BstAPI GCAn_nnn'nTGC
Cuts at: 3640 3640
Size: 6263
BstBI TT'CG_AA
Cuts at: 4624 4624
Size: 6263
BstXI CCAn_nnnn'nTGG
Cuts at: 156 547 156
Size: 391 5872
BstYI r'GATC_y
Cuts at: 1 1236 1746 1995 2034 2643 2909 2927 3288
Size: 1235 510 249 39 609 266 18 361
Cuts at: 3288 3755 3778 4113 4359 4665 4677 4763 5815
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5815 1
Size: 449
Fragments arranged by size:
1235 1052 609 510 467 449 361 335
306 266 249 246 86 39 23 18
12
Bsu36I CC'TnA_GG
Cuts at: 2860 2860
Size: 6263
BtgI C'CryG_G
Cuts at: 107 988 2269 2512 2745 107
Size: 881 1281 243 233 3625
Fragments arranged by size:
3625 1281 881 243 233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4213 4394 4557 4213
Size: 181 163 5919
Fragments arranged by size:
5919 181 163
BtsI GCAGTG_nn'
Cuts at: 3506 3506
Size: 6263
DraI TTT'AAA
Cuts at: 2552 3262 2552
Size: 710 5553
DrdI GACnn_nn'nnGTC
Cuts at: 3969 5313 3969
Size: 1344 4919
EaeI y'GGCC_r
Cuts at: 3848 4022 4413 4440 5785 3848
Size: 174 391 27 1345 4326
Fragments arranged by size:
4326 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3848 3848
Size: 6263
EarI CTCTTCn'nnn_
Cuts at: 275 737 1136 1414 1442 1508 4286 4496 275
Size: 462 399 278 28 66 2778 210 2042
Fragments arranged by size:
2778 2042 462 399 278 210 66 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 228 4471 5213 5359 6073 228
Size: 4243 742 146 714 418
Fragments arranged by size:
4243 742 714 418 146
EcoICRI GAG'CTC
Cuts at: 826 3275 826
Size: 2449 3814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 214 256 1379 1814 1878 2231 2320 2623
Size: 36 42 1123 435 64 353 89 303
Cuts at: 2623 4088 4520 4873 5535 178
Size: 1465 432 353 662 906
Fragments arranged by size:
1465 1123 906 662 435 432 353 353
303 89 64 42 36
EcoO109I rG'GnC_Cy
Cuts at: 1039 1058 1675 3032 3402 1039
Size: 19 617 1357 370 3900
Fragments arranged by size:
3900 1357 617 370 19
EcoRI G'AATT_C
Cuts at: 1333 3267 1333
Size: 1934 4329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4282 4282
Size: 6263
FspI TGC'GCA
Cuts at: 4044 4044
Size: 6263
HaeII r_GCGC'y
Cuts at: 2461 3697 3945 5175 5946 2461
Size: 1236 248 1230 771 2778
Fragments arranged by size:
2778 1236 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 815 847 1969 2001 2912 2944 4105 4135 4137
Size: 32 1122 32 911 32 1161 30 2
Cuts at: 4137 4167 815
Size: 30 2911
Fragments arranged by size:
2911 1161 1122 911 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 404 2856 3230 3302 404
Size: 2452 374 72 3365
Fragments arranged by size:
3365 2452 374 72
HindIII A'AGCT_T
Cuts at: 2822 2822
Size: 6263
HpaI GTT'AAC
Cuts at: 3230 3230
Size: 6263
KasI G'GCGC_C
Cuts at: 2457 3693 3941 2457
Size: 1236 248 4779
Fragments arranged by size:
4779 1236 248
KpnI G_GTAC'C
Cuts at: 3283 3283
Size: 6263
MluI A'CGCG_T
Cuts at: 5696 5696
Size: 6263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2345 2676 5022 5206 2345
Size: 331 2346 184 3402
Fragments arranged by size:
3402 2346 331 184
MscI TGG'CCA
Cuts at: 4024 4024
Size: 6263
MslI CAynn'nnrTG
Cuts at: 2271 4252 4379 2271
Size: 1981 127 4155
Fragments arranged by size:
4155 1981 127
MspA1I CmG'CkG
Cuts at: 356 1111 2579 2871 2891 3374 3688 4048 4832
Size: 755 1468 292 20 483 314 360 784
Cuts at: 4832 5077 356
Size: 245 1542
Fragments arranged by size:
1542 1468 784 755 483 360 314 292
245 20
NaeI GCC'GGC
Cuts at: 4444 4444
Size: 6263
NarI GG'CG_CC
Cuts at: 2458 3694 3942 2458
Size: 1236 248 4779
Fragments arranged by size:
4779 1236 248
NcoI C'CATG_G
Cuts at: 107 988 2269 2512 2745 107
Size: 881 1281 243 233 3625
Fragments arranged by size:
3625 1281 881 243 233
NdeI CA'TA_TG
Cuts at: 1134 1134
Size: 6263
NgoMIV G'CCGG_C
Cuts at: 4442 4442
Size: 6263
NspI r_CATG'y
Cuts at: 3316 3816 5419 3316
Size: 500 1603 4160
Fragments arranged by size:
4160 1603 500
PasI CC'CwG_GG
Cuts at: 1010 1010
Size: 6263
PciI A'CATG_T
Cuts at: 5415 5415
Size: 6263
PfoI T'CCnGG_A
Cuts at: 2912 2912
Size: 6263
PmeI GTTT'AAAC
Cuts at: 3262 3262
Size: 6263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4709 4741 4709
Size: 32 6231
PpuMI rG'GwC_Cy
Cuts at: 1058 1675 3032 1058
Size: 617 1357 4289
Fragments arranged by size:
4289 1357 617
PshAI GACnn'nnGTC
Cuts at: 675 2756 675
Size: 2081 4182
PsiI TTA'TAA
Cuts at: 2699 2699
Size: 6263
PspXI vC'TCGA_Gb
Cuts at: 701 701
Size: 6263
PstI C_TGCA'G
Cuts at: 1746 2331 2475 3310 3995 1746
Size: 585 144 835 685 4014
Fragments arranged by size:
4014 835 685 585 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6263
PvuII CAG'CTG
Cuts at: 356 2579 2871 3688 4048 356
Size: 2223 292 817 360 2571
Fragments arranged by size:
2571 2223 817 360 292
RsrII CG'GwC_CG
Cuts at: 4458 4458
Size: 6263
SacI G_AGCT'C
Cuts at: 828 3277 828
Size: 2449 3814
SalI G'TCGA_C
Cuts at: 3300 3300
Size: 6263
SapI GCTCTTCn'nnn_
Cuts at: 4286 4496 4286
Size: 210 6053
SbfI CC_TGCA'GG
Cuts at: 3310 3310
Size: 6263
ScaI AGT'ACT
Cuts at: 5780 5780
Size: 6263
SexAI A'CCwGG_T
Cuts at: 2954 2954
Size: 6263
SfcI C'TryA_G
Cuts at: 33 422 673 956 977 1742 2085 2327 2471
Size: 389 251 283 21 765 343 242 144
Cuts at: 2471 2847 3013 3306 3991 4959 5150 33
Size: 376 166 293 685 968 191 1146
Fragments arranged by size:
1146 968 765 685 389 376 343 293
283 251 242 191 166 144 21
SfoI GGC'GCC
Cuts at: 2459 3695 3943 2459
Size: 1236 248 4779
Fragments arranged by size:
4779 1236 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6263
SmaI CCC'GGG
Cuts at: 3285 3285
Size: 6263
SmlI C'TyrA_G
Cuts at: 377 701 730 853 1866 2533 2754 3418 4333
Size: 324 29 123 1013 667 221 664 915
Cuts at: 4333 5047 5309 377
Size: 714 262 1331
Fragments arranged by size:
1331 1013 915 714 667 664 324 262
221 123 29
SpeI A'CTAG_T
Cuts at: 67 3232 67
Size: 3165 3098
SphI G_CATG'C
Cuts at: 3316 3816 3316
Size: 500 5763
SspI AAT'ATT
Cuts at: 3057 3057
Size: 6263
StuI AGG'CCT
Cuts at: 1074 1074
Size: 6263
StyI C'CwwG_G
Cuts at: 107 540 988 1042 1061 2269 2512 2745 2761
Size: 433 448 54 19 1208 243 233 16
Cuts at: 2761 3397 3751 107
Size: 636 354 2619
Fragments arranged by size:
2619 1208 636 448 433 354 243 233
54 19 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2442 3846 3979 4438 4647 2442
Size: 1404 133 459 209 4058
Fragments arranged by size:
4058 1404 459 209 133
TatI w'GTAC_w
Cuts at: 192 521 2904 5778 192
Size: 329 2383 2874 677
Fragments arranged by size:
2874 2383 677 329
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5780 5812 5780
Size: 32 6231
Tth111I GACn'n_nGTC
Cuts at: 4060 4060
Size: 6263
XbaI T'CTAG_A
Cuts at: 2037 3294 2037
Size: 1257 5006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3758 3758
Size: 6263
XhoI C'TCGA_G
Cuts at: 701 701
Size: 6263
XmaI C'CCGG_G
Cuts at: 3283 3283
Size: 6263
XmnI GAAnn'nnTTC
Cuts at: 641 1349 3091 641
Size: 708 1742 3813
Fragments arranged by size:
3813 1742 708
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsaXI BsiWI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI MfeI NheI NotI NruI
NsiI PacI PflMI PmlI PspOMI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SwaI ZraI