(Circular) (Six-base) MAPSORT of: pF1KB3013.seq Check: 9961 from: 1 to: 4481 pF1KB3013 4481 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AccI GT'mk_AC Cuts at: 873 1325 1519 873 Size: 452 194 3835 Fragments arranged by size: 3835 452 194 Acc65I G'GTAC_C Cuts at: 1497 1497 Size: 4481 AclI AA'CG_TT Cuts at: 4321 4321 Size: 4481 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 928 2306 2738 3091 3753 928 Size: 1378 432 353 662 1656 Fragments arranged by size: 1656 1378 662 432 353 AflIII A'CryG_T Cuts at: 1833 3633 3914 1833 Size: 1800 281 2400 Fragments arranged by size: 2400 1800 281 AlwNI CAG_nnn'CTG Cuts at: 906 1251 1401 1786 3224 906 Size: 345 150 385 1438 2163 Fragments arranged by size: 2163 1438 385 345 150 ApaLI G'TGCA_C Cuts at: 3319 3319 Size: 4481 ApoI r'AATT_y Cuts at: 658 742 1001 1485 3834 4090 658 Size: 84 259 484 2349 256 1049 Fragments arranged by size: 2349 1049 484 259 256 84 AseI AT'TA_AT Cuts at: 20 478 1411 20 Size: 458 933 3090 Fragments arranged by size: 3090 933 458 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4481 AvaI C'yCGr_G Cuts at: 193 1501 193 Size: 1308 3173 BamHI G'GATC_C Cuts at: 820 1365 1506 820 Size: 545 141 3795 Fragments arranged by size: 3795 545 141 BanI G'GyrC_C Cuts at: 1497 1911 2159 2194 1497 Size: 414 248 35 3784 Fragments arranged by size: 3784 414 248 35 BanII G_rGCy'C Cuts at: 1495 2525 1495 Size: 1030 3451 BbeI G_GCGC'C Cuts at: 1915 2163 1915 Size: 248 4233 BbsI GAAGACnn'nnnn_ Cuts at: 228 228 Size: 4481 BciVI GTATCCnnnnn_n' Cuts at: 2379 3435 4481 2379 Size: 1056 1046 2379 Fragments arranged by size: 2379 1056 1046 BclI T'GATC_A Cuts at: 2001 2001 Size: 4481 BglII A'GATC_T Cuts at: 1996 4033 1996 Size: 2037 2444 BlpI GC'TnA_GC Cuts at: 1593 1593 Size: 4481 Bme1580I G_kGCm'C Cuts at: 2106 2199 3323 2106 Size: 93 1124 3264 Fragments arranged by size: 3264 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 1693 1774 2109 4253 1693 Size: 81 335 2144 1921 Fragments arranged by size: 2144 1921 335 81 BmtI G_CTAG'C Cuts at: 211 211 Size: 4481 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 612 2848 612 Size: 2236 2245 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1126 1657 2536 3250 3548 1126 Size: 531 879 714 298 2059 Fragments arranged by size: 2059 879 714 531 298 BsaI GGTCTCn'nnnn_ Cuts at: 1350 1397 4185 1350 Size: 47 2788 1646 Fragments arranged by size: 2788 1646 47 BsaAI yAC'GTr Cuts at: 1834 1834 Size: 4481 BsaBI GATnn'nnATC Cuts at: 6 1995 4032 6 Size: 1989 2037 455 Fragments arranged by size: 2037 1989 455 BsaHI Gr'CG_yC Cuts at: 1912 2160 2862 4358 1912 Size: 248 702 1496 2035 Fragments arranged by size: 2035 1496 702 248 BsaWI w'CCGG_w Cuts at: 1670 2191 2869 3280 3427 3701 1670 Size: 521 678 411 147 274 2450 Fragments arranged by size: 2450 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1468 1468 Size: 4481 BseYI C'CCAG_C Cuts at: 1906 2664 3329 3737 1906 Size: 758 665 408 2650 Fragments arranged by size: 2650 758 665 408 BsiEI CG_ry'CG Cuts at: 104 2023 2069 3299 104 Size: 1919 46 1230 1286 Fragments arranged by size: 1919 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 1495 2273 2463 3323 1495 Size: 778 190 860 2653 Fragments arranged by size: 2653 860 778 190 Bsp1286I G_dGCh'C Cuts at: 1495 2106 2199 2273 2463 2525 3323 1495 Size: 611 93 74 190 62 798 2653 Fragments arranged by size: 2653 798 611 190 93 74 62 BspHI T'CATG_A Cuts at: 2913 2913 Size: 4481 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1531 1745 2047 2428 1531 Size: 214 302 381 3584 Fragments arranged by size: 3584 381 302 214 BsrBI CCG'CTC Cuts at: 50 129 2773 2827 4311 4476 50 Size: 79 2644 54 1484 165 55 Fragments arranged by size: 2644 1484 165 79 55 54 BsrDI GCAATG_nn' Cuts at: 2393 2393 Size: 4481 BsrFI r'CCGG_y Cuts at: 2479 2660 2479 Size: 181 4300 BssSI C'ACGA_G Cuts at: 2752 3460 2752 Size: 708 3773 BstAPI GCAn_nnn'nTGC Cuts at: 1858 1858 Size: 4481 BstBI TT'CG_AA Cuts at: 708 2842 708 Size: 2134 2347 BstEII G'GTnAC_C Cuts at: 498 498 Size: 4481 BstYI r'GATC_y Cuts at: 1 820 1017 1254 1365 1506 1973 1996 2331 Size: 819 197 237 111 141 467 23 335 Cuts at: 2331 2577 2883 2895 2981 4033 1 Size: 246 306 12 86 1052 449 Fragments arranged by size: 1052 819 467 449 335 306 246 237 197 141 111 86 23 12 BstZ17I GTA'TAC Cuts at: 874 874 Size: 4481 BtgI C'CryG_G Cuts at: 107 173 107 Size: 66 4415 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2431 2612 2775 2431 Size: 181 163 4137 Fragments arranged by size: 4137 181 163 BtsI GCAGTG_nn' Cuts at: 1724 1724 Size: 4481 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1050 1085 1050 Size: 35 4446 DraI TTT'AAA Cuts at: 1025 1480 1025 Size: 455 4026 DrdI GACnn_nn'nnGTC Cuts at: 2187 3531 2187 Size: 1344 3137 EaeI y'GGCC_r Cuts at: 1205 2066 2240 2631 2658 4003 1205 Size: 861 174 391 27 1345 1683 Fragments arranged by size: 1683 1345 861 391 174 27 EagI C'GGCC_G Cuts at: 2066 2066 Size: 4481 EarI CTCTTCn'nnn_ Cuts at: 904 2504 2714 904 Size: 1600 210 2671 Fragments arranged by size: 2671 1600 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 127 402 2689 3431 3577 4291 127 Size: 275 2287 742 146 714 317 Fragments arranged by size: 2287 742 714 317 275 146 EcoICRI GAG'CTC Cuts at: 1493 1493 Size: 4481 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 928 2306 2738 3091 3753 928 Size: 1378 432 353 662 1656 Fragments arranged by size: 1656 1378 662 432 353 EcoO109I rG'GnC_Cy Cuts at: 1620 1620 Size: 4481 EcoRI G'AATT_C Cuts at: 1485 1485 Size: 4481 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 241 1120 1329 2500 241 Size: 879 209 1171 2222 Fragments arranged by size: 2222 1171 879 209 FspI TGC'GCA Cuts at: 2262 2262 Size: 4481 HaeII r_GCGC'y Cuts at: 173 189 1915 2163 3393 4164 173 Size: 16 1726 248 1230 771 490 Fragments arranged by size: 1726 1230 771 490 248 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 106 138 274 306 1452 1484 2323 2353 2355 Size: 32 136 32 1146 32 839 30 2 Cuts at: 2355 2385 106 Size: 30 2202 Fragments arranged by size: 2202 1146 839 136 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1302 1425 1520 1302 Size: 123 95 4263 Fragments arranged by size: 4263 123 95 HindIII A'AGCT_T Cuts at: 1027 1027 Size: 4481 HpaI GTT'AAC Cuts at: 1302 1425 1302 Size: 123 4358 KasI G'GCGC_C Cuts at: 1911 2159 1911 Size: 248 4233 KpnI G_GTAC'C Cuts at: 1501 1501 Size: 4481 MluI A'CGCG_T Cuts at: 3914 3914 Size: 4481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3240 3424 3240 Size: 184 4297 MscI TGG'CCA Cuts at: 1207 2242 1207 Size: 1035 3446 MslI CAynn'nnrTG Cuts at: 2470 2597 2470 Size: 127 4354 MspA1I CmG'CkG Cuts at: 804 1592 1906 2266 3050 3295 804 Size: 788 314 360 784 245 1990 Fragments arranged by size: 1990 788 784 360 314 245 NaeI GCC'GGC Cuts at: 2662 2662 Size: 4481 NarI GG'CG_CC Cuts at: 1912 2160 1912 Size: 248 4233 NcoI C'CATG_G Cuts at: 107 107 Size: 4481 NgoMIV G'CCGG_C Cuts at: 2660 2660 Size: 4481 NheI G'CTAG_C Cuts at: 207 207 Size: 4481 NspI r_CATG'y Cuts at: 1534 2034 3637 1534 Size: 500 1603 2378 Fragments arranged by size: 2378 1603 500 PciI A'CATG_T Cuts at: 3633 3633 Size: 4481 PfoI T'CCnGG_A Cuts at: 749 749 Size: 4481 PmeI GTTT'AAAC Cuts at: 1480 1480 Size: 4481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2927 2959 2927 Size: 32 4449 PstI C_TGCA'G Cuts at: 1528 2213 1528 Size: 685 3796 PvuI CG_AT'CG Cuts at: 104 104 Size: 4481 PvuII CAG'CTG Cuts at: 804 1906 2266 804 Size: 1102 360 3019 Fragments arranged by size: 3019 1102 360 RsrII CG'GwC_CG Cuts at: 2676 2676 Size: 4481 SacI G_AGCT'C Cuts at: 1495 1495 Size: 4481 SalI G'TCGA_C Cuts at: 1518 1518 Size: 4481 SapI GCTCTTCn'nnn_ Cuts at: 2504 2714 2504 Size: 210 4271 SbfI CC_TGCA'GG Cuts at: 1528 1528 Size: 4481 ScaI AGT'ACT Cuts at: 784 3998 784 Size: 3214 1267 SfcI C'TryA_G Cuts at: 33 165 846 1136 1524 2209 3177 3368 33 Size: 132 681 290 388 685 968 191 1146 Fragments arranged by size: 1146 968 685 681 388 290 191 132 SfoI GGC'GCC Cuts at: 1913 2161 1913 Size: 248 4233 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4481 SmaI CCC'GGG Cuts at: 1503 1503 Size: 4481 SmlI C'TyrA_G Cuts at: 1105 1636 2551 3265 3527 1105 Size: 531 915 714 262 2059 Fragments arranged by size: 2059 915 714 531 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4481 SphI G_CATG'C Cuts at: 1534 2034 1534 Size: 500 3981 SspI AAT'ATT Cuts at: 366 1194 366 Size: 828 3653 StyI C'CwwG_G Cuts at: 107 212 1361 1615 1969 107 Size: 105 1149 254 354 2619 Fragments arranged by size: 2619 1149 354 254 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2064 2197 2656 2865 2064 Size: 133 459 209 3680 Fragments arranged by size: 3680 459 209 133 TatI w'GTAC_w Cuts at: 433 782 918 3996 433 Size: 349 136 3078 918 Fragments arranged by size: 3078 918 349 136 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 444 476 3998 4030 444 Size: 32 3522 32 895 Fragments arranged by size: 3522 895 32 32 Tth111I GACn'n_nGTC Cuts at: 2278 2278 Size: 4481 XbaI T'CTAG_A Cuts at: 1512 1512 Size: 4481 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1976 1976 Size: 4481 XmaI C'CCGG_G Cuts at: 1501 1501 Size: 4481 XmnI GAAnn'nnTTC Cuts at: 245 1001 1098 1280 245 Size: 756 97 182 3446 Fragments arranged by size: 3446 756 182 97 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstXI Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI MfeI NdeI NotI NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI