Restriction map for
pF1KB3013
(Circular) (Six-base) MAPSORT of: pF1KB3013.seq Check: 9961 from: 1 to: 4481
pF1KB3013 4481 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 873 1325 1519 873
Size: 452 194 3835
Fragments arranged by size:
3835 452 194
Acc65I G'GTAC_C
Cuts at: 1497 1497
Size: 4481
AclI AA'CG_TT
Cuts at: 4321 4321
Size: 4481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 2306 2738 3091 3753 928
Size: 1378 432 353 662 1656
Fragments arranged by size:
1656 1378 662 432 353
AflIII A'CryG_T
Cuts at: 1833 3633 3914 1833
Size: 1800 281 2400
Fragments arranged by size:
2400 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 906 1251 1401 1786 3224 906
Size: 345 150 385 1438 2163
Fragments arranged by size:
2163 1438 385 345 150
ApaLI G'TGCA_C
Cuts at: 3319 3319
Size: 4481
ApoI r'AATT_y
Cuts at: 658 742 1001 1485 3834 4090 658
Size: 84 259 484 2349 256 1049
Fragments arranged by size:
2349 1049 484 259 256 84
AseI AT'TA_AT
Cuts at: 20 478 1411 20
Size: 458 933 3090
Fragments arranged by size:
3090 933 458
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
AvaI C'yCGr_G
Cuts at: 193 1501 193
Size: 1308 3173
BamHI G'GATC_C
Cuts at: 820 1365 1506 820
Size: 545 141 3795
Fragments arranged by size:
3795 545 141
BanI G'GyrC_C
Cuts at: 1497 1911 2159 2194 1497
Size: 414 248 35 3784
Fragments arranged by size:
3784 414 248 35
BanII G_rGCy'C
Cuts at: 1495 2525 1495
Size: 1030 3451
BbeI G_GCGC'C
Cuts at: 1915 2163 1915
Size: 248 4233
BbsI GAAGACnn'nnnn_
Cuts at: 228 228
Size: 4481
BciVI GTATCCnnnnn_n'
Cuts at: 2379 3435 4481 2379
Size: 1056 1046 2379
Fragments arranged by size:
2379 1056 1046
BclI T'GATC_A
Cuts at: 2001 2001
Size: 4481
BglII A'GATC_T
Cuts at: 1996 4033 1996
Size: 2037 2444
BlpI GC'TnA_GC
Cuts at: 1593 1593
Size: 4481
Bme1580I G_kGCm'C
Cuts at: 2106 2199 3323 2106
Size: 93 1124 3264
Fragments arranged by size:
3264 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1693 1774 2109 4253 1693
Size: 81 335 2144 1921
Fragments arranged by size:
2144 1921 335 81
BmtI G_CTAG'C
Cuts at: 211 211
Size: 4481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 612 2848 612
Size: 2236 2245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 1657 2536 3250 3548 1126
Size: 531 879 714 298 2059
Fragments arranged by size:
2059 879 714 531 298
BsaI GGTCTCn'nnnn_
Cuts at: 1350 1397 4185 1350
Size: 47 2788 1646
Fragments arranged by size:
2788 1646 47
BsaAI yAC'GTr
Cuts at: 1834 1834
Size: 4481
BsaBI GATnn'nnATC
Cuts at: 6 1995 4032 6
Size: 1989 2037 455
Fragments arranged by size:
2037 1989 455
BsaHI Gr'CG_yC
Cuts at: 1912 2160 2862 4358 1912
Size: 248 702 1496 2035
Fragments arranged by size:
2035 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1670 2191 2869 3280 3427 3701 1670
Size: 521 678 411 147 274 2450
Fragments arranged by size:
2450 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1468 1468
Size: 4481
BseYI C'CCAG_C
Cuts at: 1906 2664 3329 3737 1906
Size: 758 665 408 2650
Fragments arranged by size:
2650 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2023 2069 3299 104
Size: 1919 46 1230 1286
Fragments arranged by size:
1919 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1495 2273 2463 3323 1495
Size: 778 190 860 2653
Fragments arranged by size:
2653 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1495 2106 2199 2273 2463 2525 3323 1495
Size: 611 93 74 190 62 798 2653
Fragments arranged by size:
2653 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2913 2913
Size: 4481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1531 1745 2047 2428 1531
Size: 214 302 381 3584
Fragments arranged by size:
3584 381 302 214
BsrBI CCG'CTC
Cuts at: 50 129 2773 2827 4311 4476 50
Size: 79 2644 54 1484 165 55
Fragments arranged by size:
2644 1484 165 79 55 54
BsrDI GCAATG_nn'
Cuts at: 2393 2393
Size: 4481
BsrFI r'CCGG_y
Cuts at: 2479 2660 2479
Size: 181 4300
BssSI C'ACGA_G
Cuts at: 2752 3460 2752
Size: 708 3773
BstAPI GCAn_nnn'nTGC
Cuts at: 1858 1858
Size: 4481
BstBI TT'CG_AA
Cuts at: 708 2842 708
Size: 2134 2347
BstEII G'GTnAC_C
Cuts at: 498 498
Size: 4481
BstYI r'GATC_y
Cuts at: 1 820 1017 1254 1365 1506 1973 1996 2331
Size: 819 197 237 111 141 467 23 335
Cuts at: 2331 2577 2883 2895 2981 4033 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 819 467 449 335 306 246 237
197 141 111 86 23 12
BstZ17I GTA'TAC
Cuts at: 874 874
Size: 4481
BtgI C'CryG_G
Cuts at: 107 173 107
Size: 66 4415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2431 2612 2775 2431
Size: 181 163 4137
Fragments arranged by size:
4137 181 163
BtsI GCAGTG_nn'
Cuts at: 1724 1724
Size: 4481
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1050 1085 1050
Size: 35 4446
DraI TTT'AAA
Cuts at: 1025 1480 1025
Size: 455 4026
DrdI GACnn_nn'nnGTC
Cuts at: 2187 3531 2187
Size: 1344 3137
EaeI y'GGCC_r
Cuts at: 1205 2066 2240 2631 2658 4003 1205
Size: 861 174 391 27 1345 1683
Fragments arranged by size:
1683 1345 861 391 174 27
EagI C'GGCC_G
Cuts at: 2066 2066
Size: 4481
EarI CTCTTCn'nnn_
Cuts at: 904 2504 2714 904
Size: 1600 210 2671
Fragments arranged by size:
2671 1600 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 402 2689 3431 3577 4291 127
Size: 275 2287 742 146 714 317
Fragments arranged by size:
2287 742 714 317 275 146
EcoICRI GAG'CTC
Cuts at: 1493 1493
Size: 4481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 2306 2738 3091 3753 928
Size: 1378 432 353 662 1656
Fragments arranged by size:
1656 1378 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1620 1620
Size: 4481
EcoRI G'AATT_C
Cuts at: 1485 1485
Size: 4481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 241 1120 1329 2500 241
Size: 879 209 1171 2222
Fragments arranged by size:
2222 1171 879 209
FspI TGC'GCA
Cuts at: 2262 2262
Size: 4481
HaeII r_GCGC'y
Cuts at: 173 189 1915 2163 3393 4164 173
Size: 16 1726 248 1230 771 490
Fragments arranged by size:
1726 1230 771 490 248 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 274 306 1452 1484 2323 2353 2355
Size: 32 136 32 1146 32 839 30 2
Cuts at: 2355 2385 106
Size: 30 2202
Fragments arranged by size:
2202 1146 839 136 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1302 1425 1520 1302
Size: 123 95 4263
Fragments arranged by size:
4263 123 95
HindIII A'AGCT_T
Cuts at: 1027 1027
Size: 4481
HpaI GTT'AAC
Cuts at: 1302 1425 1302
Size: 123 4358
KasI G'GCGC_C
Cuts at: 1911 2159 1911
Size: 248 4233
KpnI G_GTAC'C
Cuts at: 1501 1501
Size: 4481
MluI A'CGCG_T
Cuts at: 3914 3914
Size: 4481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3240 3424 3240
Size: 184 4297
MscI TGG'CCA
Cuts at: 1207 2242 1207
Size: 1035 3446
MslI CAynn'nnrTG
Cuts at: 2470 2597 2470
Size: 127 4354
MspA1I CmG'CkG
Cuts at: 804 1592 1906 2266 3050 3295 804
Size: 788 314 360 784 245 1990
Fragments arranged by size:
1990 788 784 360 314 245
NaeI GCC'GGC
Cuts at: 2662 2662
Size: 4481
NarI GG'CG_CC
Cuts at: 1912 2160 1912
Size: 248 4233
NcoI C'CATG_G
Cuts at: 107 107
Size: 4481
NgoMIV G'CCGG_C
Cuts at: 2660 2660
Size: 4481
NheI G'CTAG_C
Cuts at: 207 207
Size: 4481
NspI r_CATG'y
Cuts at: 1534 2034 3637 1534
Size: 500 1603 2378
Fragments arranged by size:
2378 1603 500
PciI A'CATG_T
Cuts at: 3633 3633
Size: 4481
PfoI T'CCnGG_A
Cuts at: 749 749
Size: 4481
PmeI GTTT'AAAC
Cuts at: 1480 1480
Size: 4481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 4449
PstI C_TGCA'G
Cuts at: 1528 2213 1528
Size: 685 3796
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4481
PvuII CAG'CTG
Cuts at: 804 1906 2266 804
Size: 1102 360 3019
Fragments arranged by size:
3019 1102 360
RsrII CG'GwC_CG
Cuts at: 2676 2676
Size: 4481
SacI G_AGCT'C
Cuts at: 1495 1495
Size: 4481
SalI G'TCGA_C
Cuts at: 1518 1518
Size: 4481
SapI GCTCTTCn'nnn_
Cuts at: 2504 2714 2504
Size: 210 4271
SbfI CC_TGCA'GG
Cuts at: 1528 1528
Size: 4481
ScaI AGT'ACT
Cuts at: 784 3998 784
Size: 3214 1267
SfcI C'TryA_G
Cuts at: 33 165 846 1136 1524 2209 3177 3368 33
Size: 132 681 290 388 685 968 191 1146
Fragments arranged by size:
1146 968 685 681 388 290 191 132
SfoI GGC'GCC
Cuts at: 1913 2161 1913
Size: 248 4233
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
SmaI CCC'GGG
Cuts at: 1503 1503
Size: 4481
SmlI C'TyrA_G
Cuts at: 1105 1636 2551 3265 3527 1105
Size: 531 915 714 262 2059
Fragments arranged by size:
2059 915 714 531 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4481
SphI G_CATG'C
Cuts at: 1534 2034 1534
Size: 500 3981
SspI AAT'ATT
Cuts at: 366 1194 366
Size: 828 3653
StyI C'CwwG_G
Cuts at: 107 212 1361 1615 1969 107
Size: 105 1149 254 354 2619
Fragments arranged by size:
2619 1149 354 254 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2064 2197 2656 2865 2064
Size: 133 459 209 3680
Fragments arranged by size:
3680 459 209 133
TatI w'GTAC_w
Cuts at: 433 782 918 3996 433
Size: 349 136 3078 918
Fragments arranged by size:
3078 918 349 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 444 476 3998 4030 444
Size: 32 3522 32 895
Fragments arranged by size:
3522 895 32 32
Tth111I GACn'n_nGTC
Cuts at: 2278 2278
Size: 4481
XbaI T'CTAG_A
Cuts at: 1512 1512
Size: 4481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1976 1976
Size: 4481
XmaI C'CCGG_G
Cuts at: 1501 1501
Size: 4481
XmnI GAAnn'nnTTC
Cuts at: 245 1001 1098 1280 245
Size: 756 97 182 3446
Fragments arranged by size:
3446 756 182 97
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BglI BmgBI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstXI Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI MfeI NdeI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI