Restriction map for
pF1KB3015
(Circular) (Six-base) MAPSORT of: pF1KB3015.seq Check: 5829 from: 1 to: 5321
pF1KB3015 5321 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 918 1368 918
Size: 450 4871
AatII G_ACGT'C
Cuts at: 1175 1175
Size: 5321
AccI GT'mk_AC
Cuts at: 1851 2359 1851
Size: 508 4813
Acc65I G'GTAC_C
Cuts at: 2337 2337
Size: 5321
AclI AA'CG_TT
Cuts at: 5161 5161
Size: 5321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 992 1535 3146 3578 3931 4593 992
Size: 543 1611 432 353 662 1720
Fragments arranged by size:
1720 1611 662 543 432 353
AfeI AGC'GCT
Cuts at: 1825 1825
Size: 5321
AflIII A'CryG_T
Cuts at: 2673 4473 4754 2673
Size: 1800 281 3240
Fragments arranged by size:
3240 1800 281
AgeI A'CCGG_T
Cuts at: 251 251
Size: 5321
AleI CACnn'nnGTG
Cuts at: 1138 1138
Size: 5321
AlwNI CAG_nnn'CTG
Cuts at: 483 2626 4064 483
Size: 2143 1438 1740
Fragments arranged by size:
2143 1740 1438
ApaLI G'TGCA_C
Cuts at: 4159 4159
Size: 5321
ApoI r'AATT_y
Cuts at: 487 622 793 1113 2325 4674 4930 487
Size: 135 171 320 1212 2349 256 878
Fragments arranged by size:
2349 1212 878 320 256 171 135
AseI AT'TA_AT
Cuts at: 20 20
Size: 5321
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5321
AvaI C'yCGr_G
Cuts at: 2341 2341
Size: 5321
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1157 1190 1157
Size: 33 5288
BamHI G'GATC_C
Cuts at: 2346 2346
Size: 5321
BanI G'GyrC_C
Cuts at: 1228 2337 2751 2999 3034 1228
Size: 1109 414 248 35 3515
Fragments arranged by size:
3515 1109 414 248 35
BanII G_rGCy'C
Cuts at: 1444 2335 3365 1444
Size: 891 1030 3400
Fragments arranged by size:
3400 1030 891
BbeI G_GCGC'C
Cuts at: 2755 3003 2755
Size: 248 5073
BciVI GTATCCnnnnn_n'
Cuts at: 1789 3219 4275 5321 1789
Size: 1430 1056 1046 1789
Fragments arranged by size:
1789 1430 1056 1046
BclI T'GATC_A
Cuts at: 2841 2841
Size: 5321
BglII A'GATC_T
Cuts at: 1388 2836 4873 1388
Size: 1448 2037 1836
Fragments arranged by size:
2037 1836 1448
BlpI GC'TnA_GC
Cuts at: 2433 2433
Size: 5321
Bme1580I G_kGCm'C
Cuts at: 1006 1231 2946 3039 4163 1006
Size: 225 1715 93 1124 2164
Fragments arranged by size:
2164 1715 1124 225 93
BmrI ACTGGGnnnn_n'
Cuts at: 839 2533 2614 2949 5093 839
Size: 1694 81 335 2144 1067
Fragments arranged by size:
2144 1694 1067 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3688 3688
Size: 5321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1332 1745 2120 1332
Size: 413 375 4533
Fragments arranged by size:
4533 413 375
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 2497 3376 4090 4388 273
Size: 2224 879 714 298 1206
Fragments arranged by size:
2224 1206 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5025 5025
Size: 5321
BsaAI yAC'GTr
Cuts at: 2674 2674
Size: 5321
BsaBI GATnn'nnATC
Cuts at: 6 2835 4872 6
Size: 2829 2037 455
Fragments arranged by size:
2829 2037 455
BsaHI Gr'CG_yC
Cuts at: 1172 2752 3000 3702 5198 1172
Size: 1580 248 702 1496 1295
Fragments arranged by size:
1580 1496 1295 702 248
BsaWI w'CCGG_w
Cuts at: 251 2510 3031 3709 4120 4267 4541 251
Size: 2259 521 678 411 147 274 1031
Fragments arranged by size:
2259 1031 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1320 1350 1888 1918 1320
Size: 30 538 30 4723
Fragments arranged by size:
4723 538 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 641 689 917 641
Size: 48 228 5045
Fragments arranged by size:
5045 228 48
BseYI C'CCAG_C
Cuts at: 1568 2201 2233 2746 3504 4169 4577 1568
Size: 633 32 513 758 665 408 2312
Fragments arranged by size:
2312 758 665 633 513 408 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1802 1802
Size: 5321
BsiEI CG_ry'CG
Cuts at: 104 2863 2909 4139 104
Size: 2759 46 1230 1286
Fragments arranged by size:
2759 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2335 3113 3303 4163 2335
Size: 778 190 860 3493
Fragments arranged by size:
3493 860 778 190
BsmI GAATG_Cn'
Cuts at: 529 898 529
Size: 369 4952
BsmBI CGTCTCn'nnnn_
Cuts at: 1604 1615 1604
Size: 11 5310
Bsp1286I G_dGCh'C
Cuts at: 1006 1231 1444 2335 2946 3039 3113 3303 3365
Size: 225 213 891 611 93 74 190 62
Cuts at: 3365 4163 1006
Size: 798 2164
Fragments arranged by size:
2164 891 798 611 225 213 190 93
74 62
BspHI T'CATG_A
Cuts at: 3753 3753
Size: 5321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 659 918 1368 2371 2585 2887 3268 659
Size: 259 450 1003 214 302 381 2712
Fragments arranged by size:
2712 1003 450 381 302 259 214
BsrBI CCG'CTC
Cuts at: 50 3613 3667 5151 5316 50
Size: 3563 54 1484 165 55
Fragments arranged by size:
3563 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 968 3233 968
Size: 2265 3056
BsrFI r'CCGG_y
Cuts at: 251 2129 3319 3500 251
Size: 1878 1190 181 2072
Fragments arranged by size:
2072 1878 1190 181
BsrGI T'GTAC_A
Cuts at: 450 450
Size: 5321
BssSI C'ACGA_G
Cuts at: 3592 4300 3592
Size: 708 4613
BstAPI GCAn_nnn'nTGC
Cuts at: 2698 2698
Size: 5321
BstBI TT'CG_AA
Cuts at: 1791 3682 1791
Size: 1891 3430
BstXI CCAn_nnnn'nTGG
Cuts at: 225 310 225
Size: 85 5236
BstYI r'GATC_y
Cuts at: 1 597 987 1388 2267 2346 2813 2836 3171
Size: 596 390 401 879 79 467 23 335
Cuts at: 3171 3417 3723 3735 3821 4873 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 879 596 467 449 401 390 335
306 246 86 79 23 12
BstZ17I GTA'TAC
Cuts at: 1852 1852
Size: 5321
BtgI C'CryG_G
Cuts at: 1414 1414
Size: 5321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 224 3271 3452 3615 224
Size: 3047 181 163 1930
Fragments arranged by size:
3047 1930 181 163
BtsI GCAGTG_nn'
Cuts at: 383 506 2147 2564 383
Size: 123 1641 417 3140
Fragments arranged by size:
3140 1641 417 123
DraI TTT'AAA
Cuts at: 2320 2320
Size: 5321
DrdI GACnn_nn'nnGTC
Cuts at: 729 3027 4371 729
Size: 2298 1344 1679
Fragments arranged by size:
2298 1679 1344
EaeI y'GGCC_r
Cuts at: 1367 2906 3080 3471 3498 4843 1367
Size: 1539 174 391 27 1345 1845
Fragments arranged by size:
1845 1539 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2906 2906
Size: 5321
EarI CTCTTCn'nnn_
Cuts at: 332 335 3344 3554 332
Size: 3 3009 210 2099
Fragments arranged by size:
3009 2099 210 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3529 4271 4417 5131 3529
Size: 742 146 714 3719
Fragments arranged by size:
3719 742 714 146
EcoICRI GAG'CTC
Cuts at: 2333 2333
Size: 5321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 992 1332 1535 1745 2120 3146 3578 3931 4593
Size: 340 203 210 375 1026 432 353 662
Cuts at: 4593 992
Size: 1720
Fragments arranged by size:
1720 1026 662 432 375 353 340 210
203
EcoNI CCTnn'n_nnAGG
Cuts at: 1160 1160
Size: 5321
EcoO109I rG'GnC_Cy
Cuts at: 2460 2460
Size: 5321
EcoRI G'AATT_C
Cuts at: 2325 2325
Size: 5321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1917 3340 1917
Size: 1423 3898
FspI TGC'GCA
Cuts at: 3102 3102
Size: 5321
HaeII r_GCGC'y
Cuts at: 1827 2755 3003 4233 5004 1827
Size: 928 248 1230 771 2144
Fragments arranged by size:
2144 1230 928 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1630 1662 1717 1749 3163 3193 3195 3225 1630
Size: 32 55 32 1414 30 2 30 3726
Fragments arranged by size:
3726 1414 55 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1145 1485 1931 2360 1145
Size: 340 446 429 4106
Fragments arranged by size:
4106 446 429 340
HindIII A'AGCT_T
Cuts at: 1753 1753
Size: 5321
HpaI GTT'AAC
Cuts at: 1145 1145
Size: 5321
KasI G'GCGC_C
Cuts at: 2751 2999 2751
Size: 248 5073
KpnI G_GTAC'C
Cuts at: 2341 2341
Size: 5321
MluI A'CGCG_T
Cuts at: 4754 4754
Size: 5321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1244 4080 4264 1244
Size: 2836 184 2301
Fragments arranged by size:
2836 2301 184
MscI TGG'CCA
Cuts at: 1369 3082 1369
Size: 1713 3608
MslI CAynn'nnrTG
Cuts at: 223 1091 1138 3310 3437 223
Size: 868 47 2172 127 2107
Fragments arranged by size:
2172 2107 868 127 47
MspA1I CmG'CkG
Cuts at: 189 237 1770 2035 2432 2746 3106 3890 4135
Size: 48 1533 265 397 314 360 784 245
Cuts at: 4135 189
Size: 1375
Fragments arranged by size:
1533 1375 784 397 360 314 265 245
48
NaeI GCC'GGC
Cuts at: 3502 3502
Size: 5321
NarI GG'CG_CC
Cuts at: 2752 3000 2752
Size: 248 5073
NdeI CA'TA_TG
Cuts at: 726 1092 1587 726
Size: 366 495 4460
Fragments arranged by size:
4460 495 366
NgoMIV G'CCGG_C
Cuts at: 3500 3500
Size: 5321
NspI r_CATG'y
Cuts at: 272 2374 2874 4477 272
Size: 2102 500 1603 1116
Fragments arranged by size:
2102 1603 1116 500
PciI A'CATG_T
Cuts at: 4473 4473
Size: 5321
PfoI T'CCnGG_A
Cuts at: 908 908
Size: 5321
PmeI GTTT'AAAC
Cuts at: 2320 2320
Size: 5321
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 5289
PsiI TTA'TAA
Cuts at: 1498 1498
Size: 5321
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2087 2119 2087
Size: 32 5289
PstI C_TGCA'G
Cuts at: 513 2040 2368 3053 513
Size: 1527 328 685 2781
Fragments arranged by size:
2781 1527 685 328
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5321
PvuII CAG'CTG
Cuts at: 1770 2035 2746 3106 1770
Size: 265 711 360 3985
Fragments arranged by size:
3985 711 360 265
RsrII CG'GwC_CG
Cuts at: 254 3516 254
Size: 3262 2059
SacI G_AGCT'C
Cuts at: 2335 2335
Size: 5321
SalI G'TCGA_C
Cuts at: 2358 2358
Size: 5321
SapI GCTCTTCn'nnn_
Cuts at: 3344 3554 3344
Size: 210 5111
SbfI CC_TGCA'GG
Cuts at: 2368 2368
Size: 5321
ScaI AGT'ACT
Cuts at: 1503 1512 2029 2082 4838 1503
Size: 9 517 53 2756 1986
Fragments arranged by size:
2756 1986 517 53 9
SexAI A'CCwGG_T
Cuts at: 1449 1449
Size: 5321
SfcI C'TryA_G
Cuts at: 33 509 2036 2364 3049 4017 4208 33
Size: 476 1527 328 685 968 191 1146
Fragments arranged by size:
1527 1146 968 685 476 328 191
SfoI GGC'GCC
Cuts at: 2753 3001 2753
Size: 248 5073
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5321
SmaI CCC'GGG
Cuts at: 2343 2343
Size: 5321
SmlI C'TyrA_G
Cuts at: 288 2476 3391 4105 4367 288
Size: 2188 915 714 262 1242
Fragments arranged by size:
2188 1242 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5321
SphI G_CATG'C
Cuts at: 272 2374 2874 272
Size: 2102 500 2719
Fragments arranged by size:
2719 2102 500
SspI AAT'ATT
Cuts at: 459 459
Size: 5321
StyI C'CwwG_G
Cuts at: 2455 2809 2455
Size: 354 4967
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2904 3037 3496 3705 2904
Size: 133 459 209 4520
Fragments arranged by size:
4520 459 209 133
TatI w'GTAC_w
Cuts at: 450 1501 1510 2027 2080 2302 4836 450
Size: 1051 9 517 53 222 2534 935
Fragments arranged by size:
2534 1051 935 517 222 53 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 409 441 1030 1062 1320 1352 4838 4870 409
Size: 32 589 32 258 32 3486 32 860
Fragments arranged by size:
3486 860 589 258 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 820 3118 820
Size: 2298 3023
XbaI T'CTAG_A
Cuts at: 600 2352 600
Size: 1752 3569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 805 2816 805
Size: 2011 3310
XmaI C'CCGG_G
Cuts at: 2341 2341
Size: 5321
ZraI GAC'GTC
Cuts at: 1173 1173
Size: 5321
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PfoI PmeI PpiI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI ApaI AscI AvrII BbsI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BspEI BssHII BstEII Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PspOMI PspXI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI