Restriction map for
pF1KB3017
(Circular) (Six-base) MAPSORT of: pF1KB3017.seq Check: 1082 from: 1 to: 5507
pF1KB3017 5507 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1172 2545 1172
Size: 1373 4134
Acc65I G'GTAC_C
Cuts at: 2523 2523
Size: 5507
AclI AA'CG_TT
Cuts at: 5347 5347
Size: 5507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 953 2061 3332 3764 4117 4779 902
Size: 51 1108 1271 432 353 662 1630
Fragments arranged by size:
1630 1271 1108 662 432 353 51
AfeI AGC'GCT
Cuts at: 772 772
Size: 5507
AflIII A'CryG_T
Cuts at: 615 2859 4659 4940 615
Size: 2244 1800 281 1182
Fragments arranged by size:
2244 1800 1182 281
AleI CACnn'nnGTG
Cuts at: 1571 2236 1571
Size: 665 4842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1036 1914 2254 1036
Size: 878 340 4289
Fragments arranged by size:
4289 878 340
AlwNI CAG_nnn'CTG
Cuts at: 1182 2812 4250 1182
Size: 1630 1438 2439
Fragments arranged by size:
2439 1630 1438
ApaI G_GGCC'C
Cuts at: 1485 1712 1485
Size: 227 5280
ApaLI G'TGCA_C
Cuts at: 4345 4345
Size: 5507
ApoI r'AATT_y
Cuts at: 2511 4860 5116 2511
Size: 2349 256 2902
Fragments arranged by size:
2902 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5507
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
AvaI C'yCGr_G
Cuts at: 371 628 1930 2527 371
Size: 257 1302 597 3351
Fragments arranged by size:
3351 1302 597 257
AvrII C'CTAG_G
Cuts at: 1626 1626
Size: 5507
BamHI G'GATC_C
Cuts at: 2532 2532
Size: 5507
BanI G'GyrC_C
Cuts at: 448 463 477 848 1685 2523 2937 3185 3220
Size: 15 14 371 837 838 414 248 35
Cuts at: 3220 448
Size: 2735
Fragments arranged by size:
2735 838 837 414 371 248 35 15
14
BanII G_rGCy'C
Cuts at: 404 518 1463 1485 1695 1712 1771 1943 2521
Size: 114 945 22 210 17 59 172 578
Cuts at: 2521 3551 404
Size: 1030 2360
Fragments arranged by size:
2360 1030 945 578 210 172 114 59
22 17
BbeI G_GCGC'C
Cuts at: 452 481 852 2941 3189 452
Size: 29 371 2089 248 2770
Fragments arranged by size:
2770 2089 371 248 29
BbsI GAAGACnn'nnnn_
Cuts at: 1268 1268
Size: 5507
BbvCI CC'TCA_GC
Cuts at: 548 1373 548
Size: 825 4682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1370 1404 1370
Size: 34 5473
BciVI GTATCCnnnnn_n'
Cuts at: 547 3405 4461 5507 547
Size: 2858 1056 1046 547
Fragments arranged by size:
2858 1056 1046 547
BclI T'GATC_A
Cuts at: 3027 3027
Size: 5507
BglII A'GATC_T
Cuts at: 3022 5059 3022
Size: 2037 3470
BlpI GC'TnA_GC
Cuts at: 439 2619 439
Size: 2180 3327
Bme1580I G_kGCm'C
Cuts at: 635 1037 1485 1617 1712 2121 3132 3225 4349
Size: 402 448 132 95 409 1011 93 1124
Cuts at: 4349 635
Size: 1793
Fragments arranged by size:
1793 1124 1011 448 409 402 132 95
93
BmgBI CAC'GTC
Cuts at: 1398 1398
Size: 5507
BmrI ACTGGGnnnn_n'
Cuts at: 1019 1332 2719 2800 3135 5279 1019
Size: 313 1387 81 335 2144 1247
Fragments arranged by size:
2144 1387 1247 335 313 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1680 3874 1680
Size: 2194 3313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 415 511 787 957 1215 406
Size: 9 96 276 170 258 4698
Fragments arranged by size:
4698 276 258 170 96 9
Bpu10I CC'TnA_GC
Cuts at: 148 548 586 1373 148
Size: 400 38 787 4282
Fragments arranged by size:
4282 787 400 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2683 3562 4276 4574 2683
Size: 879 714 298 3616
Fragments arranged by size:
3616 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2244 5211 2244
Size: 2967 2540
BsaAI yAC'GTr
Cuts at: 2860 2860
Size: 5507
BsaBI GATnn'nnATC
Cuts at: 6 3021 5058 6
Size: 3015 2037 455
Fragments arranged by size:
3015 2037 455
BsaHI Gr'CG_yC
Cuts at: 173 449 478 849 2222 2938 3186 3888 5384
Size: 276 29 371 1373 716 248 702 1496
Cuts at: 5384 173
Size: 296
Fragments arranged by size:
1496 1373 716 702 371 296 276 248
29
BsaWI w'CCGG_w
Cuts at: 2696 3217 3895 4306 4453 4727 2696
Size: 521 678 411 147 274 3476
Fragments arranged by size:
3476 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 403 412 1513 373
Size: 30 9 1101 4367
Fragments arranged by size:
4367 1101 30 9
BseYI C'CCAG_C
Cuts at: 231 283 470 518 991 1550 2292 2325 2932
Size: 52 187 48 473 559 742 33 607
Cuts at: 2932 3690 4355 4763 231
Size: 758 665 408 975
Fragments arranged by size:
975 758 742 665 607 559 473 408
187 52 48 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 896 1737 2081 896
Size: 841 344 4322
Fragments arranged by size:
4322 841 344
BsiEI CG_ry'CG
Cuts at: 104 2237 3049 3095 4325 104
Size: 2133 812 46 1230 1286
Fragments arranged by size:
2133 1286 1230 812 46
BsiHKAI G_wGCw'C
Cuts at: 727 1648 1771 2007 2243 2521 3299 3489 4349
Size: 921 123 236 236 278 778 190 860
Cuts at: 4349 727
Size: 1885
Fragments arranged by size:
1885 921 860 778 278 236 236 190
123
BsmI GAATG_Cn'
Cuts at: 1903 1903
Size: 5507
Bsp1286I G_dGCh'C
Cuts at: 404 518 635 727 1037 1463 1485 1617 1648
Size: 114 117 92 310 426 22 132 31
Cuts at: 1648 1695 1712 1771 1943 2007 2121 2243 2521
Size: 47 17 59 172 64 114 122 278
Cuts at: 2521 3132 3225 3299 3489 3551 4349 404
Size: 611 93 74 190 62 798 1562
Fragments arranged by size:
1562 798 611 426 310 278 190 172
132 122 117 114 114 93 92 74
64 62 59 47 31 22 17
BspHI T'CATG_A
Cuts at: 3939 3939
Size: 5507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2557 2771 3073 3454 2557
Size: 214 302 381 4610
Fragments arranged by size:
4610 381 302 214
BsrBI CCG'CTC
Cuts at: 50 438 837 2178 3799 3853 5337 5502 50
Size: 388 399 1341 1621 54 1484 165 55
Fragments arranged by size:
1621 1484 1341 399 388 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1059 3419 1059
Size: 2360 3147
BsrFI r'CCGG_y
Cuts at: 2345 3505 3686 2345
Size: 1160 181 4166
Fragments arranged by size:
4166 1160 181
BssSI C'ACGA_G
Cuts at: 3778 4486 3778
Size: 708 4799
BstAPI GCAn_nnn'nTGC
Cuts at: 420 639 1826 1892 1976 2884 420
Size: 219 1187 66 84 908 3043
Fragments arranged by size:
3043 1187 908 219 84 66
BstBI TT'CG_AA
Cuts at: 3868 3868
Size: 5507
BstXI CCAn_nnnn'nTGG
Cuts at: 474 765 991 1239 2292 474
Size: 291 226 248 1053 3689
Fragments arranged by size:
3689 1053 291 248 226
BstYI r'GATC_y
Cuts at: 1 179 2532 2999 3022 3357 3603 3909 3921
Size: 178 2353 467 23 335 246 306 12
Cuts at: 3921 4007 5059 1
Size: 86 1052 449
Fragments arranged by size:
2353 1052 467 449 335 306 246 178
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1471 1471
Size: 5507
BtgI C'CryG_G
Cuts at: 107 405 453 107
Size: 298 48 5161
Fragments arranged by size:
5161 298 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3457 3638 3801 3457
Size: 181 163 5163
Fragments arranged by size:
5163 181 163
BtsI GCAGTG_nn'
Cuts at: 1035 1162 1446 1548 1566 1578 1725 1764 1908
Size: 127 284 102 18 12 147 39 144
Cuts at: 1908 1953 2324 2750 1035
Size: 45 371 426 3792
Fragments arranged by size:
3792 426 371 284 147 144 127 102
45 39 18 12
DraI TTT'AAA
Cuts at: 2506 2506
Size: 5507
DraIII CAC_nnn'GTG
Cuts at: 801 1526 801
Size: 725 4782
DrdI GACnn_nn'nnGTC
Cuts at: 3213 4557 3213
Size: 1344 4163
EaeI y'GGCC_r
Cuts at: 408 1578 1620 2093 3092 3266 3657 3684 5029
Size: 1170 42 473 999 174 391 27 1345
Cuts at: 5029 408
Size: 886
Fragments arranged by size:
1345 1170 999 886 473 391 174 42
27
EagI C'GGCC_G
Cuts at: 3092 3092
Size: 5507
EarI CTCTTCn'nnn_
Cuts at: 1305 1351 3530 3740 1305
Size: 46 2179 210 3072
Fragments arranged by size:
3072 2179 210 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 225 346 3715 4457 4603 5317 225
Size: 121 3369 742 146 714 415
Fragments arranged by size:
3369 742 714 415 146 121
EcoICRI GAG'CTC
Cuts at: 1769 2519 1769
Size: 750 4757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 415 511 787 902 953 957 1215 2061
Size: 9 96 276 115 51 4 258 846
Cuts at: 2061 3332 3764 4117 4779 406
Size: 1271 432 353 662 1134
Fragments arranged by size:
1271 1134 846 662 432 353 276 258
115 96 51 9 4
EcoO109I rG'GnC_Cy
Cuts at: 236 1113 1348 1482 1708 1709 2072 2646 236
Size: 877 235 134 226 1 363 574 3097
Fragments arranged by size:
3097 877 574 363 235 226 134 1
EcoRI G'AATT_C
Cuts at: 2511 2511
Size: 5507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3526 3526
Size: 5507
FspI TGC'GCA
Cuts at: 676 3288 676
Size: 2612 2895
FspAI rTGC'GCAy
Cuts at: 676 676
Size: 5507
HaeII r_GCGC'y
Cuts at: 345 452 481 774 852 2941 3189 4419 5190
Size: 107 29 293 78 2089 248 1230 771
Cuts at: 5190 345
Size: 662
Fragments arranged by size:
2089 1230 771 662 293 248 107 78
29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1663 1695 2219 2251 3349 3379 3381 3411 1663
Size: 32 524 32 1098 30 2 30 3759
Fragments arranged by size:
3759 1098 524 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2546 2546
Size: 5507
KasI G'GCGC_C
Cuts at: 448 477 848 2937 3185 448
Size: 29 371 2089 248 2770
Fragments arranged by size:
2770 2089 371 248 29
KpnI G_GTAC'C
Cuts at: 2527 2527
Size: 5507
MluI A'CGCG_T
Cuts at: 615 4940 615
Size: 4325 1182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4266 4450 4266
Size: 184 5323
MscI TGG'CCA
Cuts at: 1580 2095 3268 1580
Size: 515 1173 3819
Fragments arranged by size:
3819 1173 515
MslI CAynn'nnrTG
Cuts at: 1018 1571 1868 1896 2126 2236 3496 3623 1018
Size: 553 297 28 230 110 1260 127 2902
Fragments arranged by size:
2902 1260 553 297 230 127 110 28
MspA1I CmG'CkG
Cuts at: 287 384 407 455 474 486 991 1610 1754
Size: 97 23 48 19 12 505 619 144
Cuts at: 1754 1802 1976 2196 2618 2932 3292 4076 4321
Size: 48 174 220 422 314 360 784 245
Cuts at: 4321 287
Size: 1473
Fragments arranged by size:
1473 784 619 505 422 360 314 245
220 174 144 97 48 48 23 19
12
NaeI GCC'GGC
Cuts at: 3688 3688
Size: 5507
NarI GG'CG_CC
Cuts at: 449 478 849 2938 3186 449
Size: 29 371 2089 248 2770
Fragments arranged by size:
2770 2089 371 248 29
NcoI C'CATG_G
Cuts at: 107 107
Size: 5507
NgoMIV G'CCGG_C
Cuts at: 3686 3686
Size: 5507
NspI r_CATG'y
Cuts at: 1895 2560 3060 4663 1895
Size: 665 500 1603 2739
Fragments arranged by size:
2739 1603 665 500
PciI A'CATG_T
Cuts at: 4659 4659
Size: 5507
PflMI CCAn_nnn'nTGG
Cuts at: 206 1986 206
Size: 1780 3727
PmeI GTTT'AAAC
Cuts at: 2506 2506
Size: 5507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 3953
Size: 32 5475
PpuMI rG'GwC_Cy
Cuts at: 236 1348 236
Size: 1112 4395
PsiI TTA'TAA
Cuts at: 1265 1265
Size: 5507
PspOMI G'GGCC_C
Cuts at: 1481 1708 1481
Size: 227 5280
PspXI vC'TCGA_Gb
Cuts at: 371 371
Size: 5507
PstI C_TGCA'G
Cuts at: 428 656 908 1125 1209 1573 1753 1759 1948
Size: 228 252 217 84 364 180 6 189
Cuts at: 1948 2107 2554 3239 428
Size: 159 447 685 2696
Fragments arranged by size:
2696 685 447 364 252 228 217 189
180 159 84 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5507
PvuII CAG'CTG
Cuts at: 287 474 991 1610 1754 1802 2196 2932 3292
Size: 187 517 619 144 48 394 736 360
Cuts at: 3292 287
Size: 2502
Fragments arranged by size:
2502 736 619 517 394 360 187 144
48
RsrII CG'GwC_CG
Cuts at: 3702 3702
Size: 5507
SacI G_AGCT'C
Cuts at: 1771 2521 1771
Size: 750 4757
SacII CC_GC'GG
Cuts at: 408 456 408
Size: 48 5459
SalI G'TCGA_C
Cuts at: 2544 2544
Size: 5507
SapI GCTCTTCn'nnn_
Cuts at: 3530 3740 3530
Size: 210 5297
SbfI CC_TGCA'GG
Cuts at: 2554 2554
Size: 5507
ScaI AGT'ACT
Cuts at: 5024 5024
Size: 5507
SfcI C'TryA_G
Cuts at: 33 424 652 904 1062 1121 1205 1569 1749
Size: 391 228 252 158 59 84 364 180
Cuts at: 1749 1755 1944 2103 2550 3235 4203 4394 33
Size: 6 189 159 447 685 968 191 1146
Fragments arranged by size:
1146 968 685 447 391 364 252 228
191 189 180 159 158 84 59 6
SfoI GGC'GCC
Cuts at: 450 479 850 2939 3187 450
Size: 29 371 2089 248 2770
Fragments arranged by size:
2770 2089 371 248 29
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
SmaI CCC'GGG
Cuts at: 2529 2529
Size: 5507
SmlI C'TyrA_G
Cuts at: 371 2662 3577 4291 4553 371
Size: 2291 915 714 262 1325
Fragments arranged by size:
2291 1325 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5507
SphI G_CATG'C
Cuts at: 1895 2560 3060 1895
Size: 665 500 4342
Fragments arranged by size:
4342 665 500
StyI C'CwwG_G
Cuts at: 107 1485 1626 2141 2641 2995 107
Size: 1378 141 515 500 354 2619
Fragments arranged by size:
2619 1378 515 500 354 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 484 679 1459 1564 1673 3090 3223 3682 3891
Size: 195 780 105 109 1417 133 459 209
Cuts at: 3891 484
Size: 2100
Fragments arranged by size:
2100 1417 780 459 209 195 133 109
105
TatI w'GTAC_w
Cuts at: 569 2107 5022 569
Size: 1538 2915 1054
Fragments arranged by size:
2915 1538 1054
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 545 577 1669 1701 2451 2483 5024 5056 545
Size: 32 1092 32 750 32 2541 32 996
Fragments arranged by size:
2541 1092 996 750 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3304 3304
Size: 5507
XbaI T'CTAG_A
Cuts at: 2538 2538
Size: 5507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 928 1573 2133 3002 928
Size: 645 560 869 3433
Fragments arranged by size:
3433 869 645 560
XhoI C'TCGA_G
Cuts at: 371 371
Size: 5507
XmaI C'CCGG_G
Cuts at: 2527 2527
Size: 5507
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AloI AscI BaeI
BfrBI BglI BmtI BsaXI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI