Restriction map for
pF1KB3020
(Circular) (Six-base) MAPSORT of: pF1KB3020.seq Check: 4970 from: 1 to: 5036
pF1KB3020 5036 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1158 1398 1158
Size: 240 4796
AccI GT'mk_AC
Cuts at: 2074 2074
Size: 5036
Acc65I G'GTAC_C
Cuts at: 600 2052 600
Size: 1452 3584
AclI AA'CG_TT
Cuts at: 4876 4876
Size: 5036
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1585 1711 2042 2861 3293 4040 4308 1145
Size: 440 126 331 819 432 747 268 1873
Fragments arranged by size:
1873 819 747 440 432 331 268 126
AflIII A'CryG_T
Cuts at: 1925 2388 3492 4469 1925
Size: 463 1104 977 2492
Fragments arranged by size:
2492 1104 977 463
AgeI A'CCGG_T
Cuts at: 3424 3424
Size: 5036
AhdI GACnn_n'nnGTC
Cuts at: 1299 1299
Size: 5036
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 199 505 199
Size: 306 4730
AlwNI CAG_nnn'CTG
Cuts at: 597 1604 2341 3908 597
Size: 1007 737 1567 1725
Fragments arranged by size:
1725 1567 1007 737
ApaI G_GGCC'C
Cuts at: 1586 1873 1891 1586
Size: 287 18 4731
Fragments arranged by size:
4731 287 18
ApaLI G'TGCA_C
Cuts at: 1411 3806 1411
Size: 2395 2641
ApoI r'AATT_y
Cuts at: 2040 4389 4645 2040
Size: 2349 256 2431
Fragments arranged by size:
2431 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5036
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
AvaI C'yCGr_G
Cuts at: 746 1466 1864 1936 2056 746
Size: 720 398 72 120 3726
Fragments arranged by size:
3726 720 398 120 72
BamHI G'GATC_C
Cuts at: 1638 2061 1638
Size: 423 4613
BanI G'GyrC_C
Cuts at: 600 976 1509 2052 2466 2714 2749 600
Size: 376 533 543 414 248 35 2887
Fragments arranged by size:
2887 543 533 414 376 248 35
BanII G_rGCy'C
Cuts at: 1586 1654 1863 1873 1891 2050 3080 1586
Size: 68 209 10 18 159 1030 3542
Fragments arranged by size:
3542 1030 209 159 68 18 10
BbeI G_GCGC'C
Cuts at: 2470 2718 2470
Size: 248 4788
BbsI GAAGACnn'nnnn_
Cuts at: 1133 1508 1699 1133
Size: 375 191 4470
Fragments arranged by size:
4470 375 191
BbvCI CC'TCA_GC
Cuts at: 1916 1916
Size: 5036
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 693 727 693
Size: 34 5002
BciVI GTATCCnnnnn_n'
Cuts at: 2934 3695 5036 2934
Size: 761 1341 2934
Fragments arranged by size:
2934 1341 761
BclI T'GATC_A
Cuts at: 1383 2556 1383
Size: 1173 3863
BglI GCCn_nnn'nGGC
Cuts at: 143 283 2016 143
Size: 140 1733 3163
Fragments arranged by size:
3163 1733 140
BglII A'GATC_T
Cuts at: 557 1181 1193 2551 4588 557
Size: 624 12 1358 2037 1005
Fragments arranged by size:
2037 1358 1005 624 12
BlpI GC'TnA_GC
Cuts at: 2148 2148
Size: 5036
Bme1580I G_kGCm'C
Cuts at: 882 1415 1514 1586 1873 1891 2661 2754 3810
Size: 533 99 72 287 18 770 93 1056
Cuts at: 3810 882
Size: 2108
Fragments arranged by size:
2108 1056 770 533 287 99 93 72
18
BmgBI CAC'GTC
Cuts at: 234 234
Size: 5036
BmrI ACTGGGnnnn_n'
Cuts at: 318 857 2248 2329 2664 4808 318
Size: 539 1391 81 335 2144 546
Fragments arranged by size:
2144 1391 546 539 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3403 3403
Size: 5036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1048 1359 439
Size: 609 311 4116
Fragments arranged by size:
4116 609 311
Bpu10I CC'TnA_GC
Cuts at: 1916 1916
Size: 5036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2212 3091 3583 3881 2212
Size: 879 492 298 3367
Fragments arranged by size:
3367 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1463 4740 1463
Size: 3277 1759
BsaAI yAC'GTr
Cuts at: 2389 2389
Size: 5036
BsaBI GATnn'nnATC
Cuts at: 6 1208 2550 4587 6
Size: 1202 1342 2037 455
Fragments arranged by size:
2037 1342 1202 455
BsaHI Gr'CG_yC
Cuts at: 1696 2467 2715 3417 4913 1696
Size: 771 248 702 1496 1819
Fragments arranged by size:
1819 1496 771 702 248
BsaWI w'CCGG_w
Cuts at: 2225 2746 3424 3698 3845 4256 2225
Size: 521 678 274 147 411 3005
Fragments arranged by size:
3005 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1765 1795 1765
Size: 30 5006
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 748 751 1079 1164 1904 1924 748
Size: 3 328 85 740 20 3860
Fragments arranged by size:
3860 740 328 85 20 3
BseYI C'CCAG_C
Cuts at: 264 1043 1068 1098 1585 2461 3219 3796 4292
Size: 779 25 30 487 876 758 577 496
Cuts at: 4292 264
Size: 1008
Fragments arranged by size:
1008 876 779 758 577 496 487 30
25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 779 172
Size: 607 4429
BsiEI CG_ry'CG
Cuts at: 104 524 2578 2624 3832 104
Size: 420 2054 46 1208 1308
Fragments arranged by size:
2054 1308 1208 420 46
BsiHKAI G_wGCw'C
Cuts at: 239 1415 1654 2050 2828 3018 3810 239
Size: 1176 239 396 778 190 792 1465
Fragments arranged by size:
1465 1176 792 778 396 239 190
Bsp1286I G_dGCh'C
Cuts at: 239 882 1415 1514 1586 1654 1863 1873 1891
Size: 643 533 99 72 68 209 10 18
Cuts at: 1891 2050 2661 2754 2828 3018 3080 3810 239
Size: 159 611 93 74 190 62 730 1465
Fragments arranged by size:
1465 730 643 611 533 209 190 159
99 93 74 72 68 62 18 10
BspEI T'CCGG_A
Cuts at: 4256 4256
Size: 5036
BspHI T'CATG_A
Cuts at: 515 1497 4212 515
Size: 982 2715 1339
Fragments arranged by size:
2715 1339 982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 938 1158 1398 1453 2086 2300 2602 2983 938
Size: 220 240 55 633 214 302 381 2991
Fragments arranged by size:
2991 633 381 302 240 220 214 55
BsrBI CCG'CTC
Cuts at: 50 114 1425 1814 3328 3382 4866 5031 50
Size: 64 1311 389 1514 54 1484 165 55
Fragments arranged by size:
1514 1484 1311 389 165 64 55 54
BsrDI GCAATG_nn'
Cuts at: 711 764 1455 2948 711
Size: 53 691 1493 2799
Fragments arranged by size:
2799 1493 691 53
BsrFI r'CCGG_y
Cuts at: 715 1022 1220 3034 3215 3424 715
Size: 307 198 1814 181 209 2327
Fragments arranged by size:
2327 1814 307 209 198 181
BssSI C'ACGA_G
Cuts at: 1036 3307 3665 1036
Size: 2271 358 2407
Fragments arranged by size:
2407 2271 358
BstAPI GCAn_nnn'nTGC
Cuts at: 766 877 1595 2413 766
Size: 111 718 818 3389
Fragments arranged by size:
3389 818 718 111
BstBI TT'CG_AA
Cuts at: 3397 3397
Size: 5036
BstEII G'GTnAC_C
Cuts at: 1666 1666
Size: 5036
BstXI CCAn_nnnn'nTGG
Cuts at: 933 1360 1525 933
Size: 427 165 4444
Fragments arranged by size:
4444 427 165
BstYI r'GATC_y
Cuts at: 1 557 593 1181 1193 1638 1989 2061 2528
Size: 556 36 588 12 445 351 72 467
Cuts at: 2528 2551 2886 3132 4144 4230 4242 4588 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 588 556 467 449 445 351 346
335 246 86 72 36 23 12 12
Bsu36I CC'TnA_GG
Cuts at: 964 1994 964
Size: 1030 4006
BtgI C'CryG_G
Cuts at: 107 815 1331 2012 107
Size: 708 516 681 3131
Fragments arranged by size:
3131 708 681 516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 696 2986 3167 3330 696
Size: 2290 181 163 2402
Fragments arranged by size:
2402 2290 181 163
BtsI GCAGTG_nn'
Cuts at: 2279 2279
Size: 5036
DraI TTT'AAA
Cuts at: 2035 2035
Size: 5036
DrdI GACnn_nn'nnGTC
Cuts at: 1741 2742 3600 1741
Size: 1001 858 3177
Fragments arranged by size:
3177 1001 858
EaeI y'GGCC_r
Cuts at: 284 521 2621 2795 3186 3213 4558 284
Size: 237 2100 174 391 27 1345 762
Fragments arranged by size:
2100 1345 762 391 237 174 27
EagI C'GGCC_G
Cuts at: 521 2621 521
Size: 2100 2936
EarI CTCTTCn'nnn_
Cuts at: 3059 3269 3059
Size: 210 4826
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 353 3244 3554 3700 4846 353
Size: 2891 310 146 1146 543
Fragments arranged by size:
2891 1146 543 310 146
EcoICRI GAG'CTC
Cuts at: 1652 2048 1652
Size: 396 4640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1048 1145 1359 1585 1711 2042 2861 3293
Size: 609 97 214 226 126 331 819 432
Cuts at: 3293 4040 4308 439
Size: 747 268 1167
Fragments arranged by size:
1167 819 747 609 432 331 268 226
214 126 97
EcoNI CCTnn'n_nnAGG
Cuts at: 967 967
Size: 5036
EcoO109I rG'GnC_Cy
Cuts at: 644 950 960 1582 1869 1870 1881 1888 2017
Size: 306 10 622 287 1 11 7 129
Cuts at: 2017 2175 644
Size: 158 3505
Fragments arranged by size:
3505 622 306 287 158 129 11 10
7 1
EcoRI G'AATT_C
Cuts at: 2040 2040
Size: 5036
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1842 3055 1842
Size: 1213 3823
FspI TGC'GCA
Cuts at: 2817 2817
Size: 5036
HaeII r_GCGC'y
Cuts at: 225 323 2470 2718 3740 4719 225
Size: 98 2147 248 1022 979 542
Fragments arranged by size:
2147 1022 979 542 248 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1285 1317 2878 2908 2910 2940 1285
Size: 32 1561 30 2 30 3381
Fragments arranged by size:
3381 1561 32 30 30 2
HincII GTy'rAC
Cuts at: 2001 2075 2001
Size: 74 4962
KasI G'GCGC_C
Cuts at: 2466 2714 2466
Size: 248 4788
KpnI G_GTAC'C
Cuts at: 604 2056 604
Size: 1452 3584
MluI A'CGCG_T
Cuts at: 4469 4469
Size: 5036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 608 3707 3891 608
Size: 3099 184 1753
Fragments arranged by size:
3099 1753 184
MscI TGG'CCA
Cuts at: 2797 2797
Size: 5036
MslI CAynn'nnrTG
Cuts at: 181 487 3025 3152 181
Size: 306 2538 127 2065
Fragments arranged by size:
2538 2065 306 127
MspA1I CmG'CkG
Cuts at: 1068 2147 2461 2821 3834 4079 1068
Size: 1079 314 360 1013 245 2025
Fragments arranged by size:
2025 1079 1013 360 314 245
NaeI GCC'GGC
Cuts at: 717 3217 717
Size: 2500 2536
NarI GG'CG_CC
Cuts at: 2467 2715 2467
Size: 248 4788
NcoI C'CATG_G
Cuts at: 107 815 2012 107
Size: 708 1197 3131
Fragments arranged by size:
3131 1197 708
NgoMIV G'CCGG_C
Cuts at: 715 3215 715
Size: 2500 2536
NspI r_CATG'y
Cuts at: 726 1302 1855 1929 2089 2589 3496 726
Size: 576 553 74 160 500 907 2266
Fragments arranged by size:
2266 907 576 553 500 160 74
PasI CC'CwG_GG
Cuts at: 640 955 1875 640
Size: 315 920 3801
Fragments arranged by size:
3801 920 315
PciI A'CATG_T
Cuts at: 1925 3492 1925
Size: 1567 3469
PmeI GTTT'AAAC
Cuts at: 2035 2035
Size: 5036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 298 330 4175 4207 298
Size: 32 3845 32 1127
Fragments arranged by size:
3845 1127 32 32
PpuMI rG'GwC_Cy
Cuts at: 644 950 1881 644
Size: 306 931 3799
Fragments arranged by size:
3799 931 306
PshAI GACnn'nnGTC
Cuts at: 353 353
Size: 5036
PspOMI G'GGCC_C
Cuts at: 1582 1869 1887 1582
Size: 287 18 4731
Fragments arranged by size:
4731 287 18
PspXI vC'TCGA_Gb
Cuts at: 1936 1936
Size: 5036
PstI C_TGCA'G
Cuts at: 295 1600 2083 2768 295
Size: 1305 483 685 2563
Fragments arranged by size:
2563 1305 685 483
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5036
PvuII CAG'CTG
Cuts at: 1068 2461 2821 1068
Size: 1393 360 3283
Fragments arranged by size:
3283 1393 360
RsrII CG'GwC_CG
Cuts at: 3231 3231
Size: 5036
SacI G_AGCT'C
Cuts at: 1654 2050 1654
Size: 396 4640
SalI G'TCGA_C
Cuts at: 2073 2073
Size: 5036
SanDI GG'GwC_CC
Cuts at: 950 950
Size: 5036
SapI GCTCTTCn'nnn_
Cuts at: 3059 3269 3059
Size: 210 4826
SbfI CC_TGCA'GG
Cuts at: 2083 2083
Size: 5036
ScaI AGT'ACT
Cuts at: 301 1534 4553 301
Size: 1233 3019 784
Fragments arranged by size:
3019 1233 784
SexAI A'CCwGG_T
Cuts at: 158 158
Size: 5036
SfcI C'TryA_G
Cuts at: 33 291 1596 2079 2764 3757 3948 33
Size: 258 1305 483 685 993 191 1121
Fragments arranged by size:
1305 1121 993 685 483 258 191
SfoI GGC'GCC
Cuts at: 2468 2716 2468
Size: 248 4788
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
SmaI CCC'GGG
Cuts at: 2058 2058
Size: 5036
SmlI C'TyrA_G
Cuts at: 1936 2191 3106 3598 3860 1936
Size: 255 915 492 262 3112
Fragments arranged by size:
3112 915 492 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5036
SphI G_CATG'C
Cuts at: 726 2089 2589 726
Size: 1363 500 3173
Fragments arranged by size:
3173 1363 500
StuI AGG'CCT
Cuts at: 1380 1380
Size: 5036
StyI C'CwwG_G
Cuts at: 107 440 689 815 916 1896 2012 2170 2524
Size: 333 249 126 101 980 116 158 354
Cuts at: 2524 107
Size: 2619
Fragments arranged by size:
2619 980 354 333 249 158 126 116
101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1090 2619 2752 3211 3420 1090
Size: 1529 133 459 209 2706
Fragments arranged by size:
2706 1529 459 209 133
TatI w'GTAC_w
Cuts at: 299 1532 4551 299
Size: 1233 3019 784
Fragments arranged by size:
3019 1233 784
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1216 1248 4553 4585 1216
Size: 32 3305 32 1667
Fragments arranged by size:
3305 1667 32 32
Tth111I GACn'n_nGTC
Cuts at: 2833 2833
Size: 5036
XbaI T'CTAG_A
Cuts at: 2067 2067
Size: 5036
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1065 1167 1755 2531 1065
Size: 102 588 776 3570
Fragments arranged by size:
3570 776 588 102
XhoI C'TCGA_G
Cuts at: 1936 1936
Size: 5036
XmaI C'CCGG_G
Cuts at: 2056 2056
Size: 5036
XmnI GAAnn'nnTTC
Cuts at: 1369 1369
Size: 5036
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PsiI PsrI SacII SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI