Restriction map for
pF1KB3021
(Circular) (Six-base) MAPSORT of: pF1KB3021.seq Check: 2381 from: 1 to: 5897
pF1KB3021 5897 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1350 2935 1350
Size: 1585 4312
Acc65I G'GTAC_C
Cuts at: 2913 2913
Size: 5897
AclI AA'CG_TT
Cuts at: 5737 5737
Size: 5897
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 576 1120 1280 2554 2717 3722 4154 4507 5169
Size: 544 160 1274 163 1005 432 353 662
Cuts at: 5169 576
Size: 1304
Fragments arranged by size:
1304 1274 1005 662 544 432 353 163
160
AflIII A'CryG_T
Cuts at: 610 1966 2328 3249 5049 5330 610
Size: 1356 362 921 1800 281 1177
Fragments arranged by size:
1800 1356 1177 921 362 281
AlwNI CAG_nnn'CTG
Cuts at: 2397 2808 3202 4640 2397
Size: 411 394 1438 3654
Fragments arranged by size:
3654 1438 411 394
ApaLI G'TGCA_C
Cuts at: 887 4735 887
Size: 3848 2049
ApoI r'AATT_y
Cuts at: 496 663 990 1008 1076 1275 1393 1521 1974
Size: 167 327 18 68 199 118 128 453
Cuts at: 1974 2266 2549 2794 2901 5250 5506 496
Size: 292 283 245 107 2349 256 887
Fragments arranged by size:
2349 887 453 327 292 283 256 245
199 167 128 118 107 68 18
AseI AT'TA_AT
Cuts at: 20 845 1261 20
Size: 825 416 4656
Fragments arranged by size:
4656 825 416
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5897
AvaI C'yCGr_G
Cuts at: 2917 2917
Size: 5897
BamHI G'GATC_C
Cuts at: 2922 2922
Size: 5897
BanI G'GyrC_C
Cuts at: 171 2913 3327 3575 3610 171
Size: 2742 414 248 35 2458
Fragments arranged by size:
2742 2458 414 248 35
BanII G_rGCy'C
Cuts at: 2911 3941 2911
Size: 1030 4867
BbeI G_GCGC'C
Cuts at: 3331 3579 3331
Size: 248 5649
BbsI GAAGACnn'nnnn_
Cuts at: 2376 2376
Size: 5897
BbvCI CC'TCA_GC
Cuts at: 2007 2007
Size: 5897
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5863
BciVI GTATCCnnnnn_n'
Cuts at: 1369 2862 3795 4851 5897 1369
Size: 1493 933 1056 1046 1369
Fragments arranged by size:
1493 1369 1056 1046 933
BclI T'GATC_A
Cuts at: 1287 1857 2336 3417 1287
Size: 570 479 1081 3767
Fragments arranged by size:
3767 1081 570 479
BglII A'GATC_T
Cuts at: 1095 1939 3412 5449 1095
Size: 844 1473 2037 1543
Fragments arranged by size:
2037 1543 1473 844
BlpI GC'TnA_GC
Cuts at: 3009 3009
Size: 5897
Bme1580I G_kGCm'C
Cuts at: 891 3522 3615 4739 891
Size: 2631 93 1124 2049
Fragments arranged by size:
2631 2049 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3109 3190 3525 5669 3109
Size: 81 335 2144 3337
Fragments arranged by size:
3337 2144 335 81
BmtI G_CTAG'C
Cuts at: 2080 2080
Size: 5897
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4264 4264
Size: 5897
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1211 1879 2528 1211
Size: 668 649 4580
Fragments arranged by size:
4580 668 649
Bpu10I CC'TnA_GC
Cuts at: 2007 2007
Size: 5897
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1600 3073 3952 4666 4964 268
Size: 1332 1473 879 714 298 1201
Fragments arranged by size:
1473 1332 1201 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5601 5601
Size: 5897
BsaAI yAC'GTr
Cuts at: 3250 3250
Size: 5897
BsaBI GATnn'nnATC
Cuts at: 6 1922 3411 5448 6
Size: 1916 1489 2037 455
Fragments arranged by size:
2037 1916 1489 455
BsaHI Gr'CG_yC
Cuts at: 3328 3576 4278 5774 3328
Size: 248 702 1496 3451
Fragments arranged by size:
3451 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3086 3607 4285 4696 4843 5117 3086
Size: 521 678 411 147 274 3866
Fragments arranged by size:
3866 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 2362 211
Size: 2151 3746
BseYI C'CCAG_C
Cuts at: 637 3322 4080 4745 5153 637
Size: 2685 758 665 408 1381
Fragments arranged by size:
2685 1381 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1311 1311
Size: 5897
BsiEI CG_ry'CG
Cuts at: 104 137 2676 3439 3485 4715 104
Size: 33 2539 763 46 1230 1286
Fragments arranged by size:
2539 1286 1230 763 46 33
BsiHKAI G_wGCw'C
Cuts at: 891 2126 2684 2911 3689 3879 4739 891
Size: 1235 558 227 778 190 860 2049
Fragments arranged by size:
2049 1235 860 778 558 227 190
BsmI GAATG_Cn'
Cuts at: 2843 2843
Size: 5897
Bsp1286I G_dGCh'C
Cuts at: 891 2126 2684 2911 3522 3615 3689 3879 3941
Size: 1235 558 227 611 93 74 190 62
Cuts at: 3941 4739 891
Size: 798 2049
Fragments arranged by size:
2049 1235 798 611 558 227 190 93
74 62
BspHI T'CATG_A
Cuts at: 1122 2189 4329 1122
Size: 1067 2140 2690
Fragments arranged by size:
2690 2140 1067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 239 2947 3161 3463 3844 239
Size: 2708 214 302 381 2292
Fragments arranged by size:
2708 2292 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4189 4243 5727 5892 50
Size: 4139 54 1484 165 55
Fragments arranged by size:
4139 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3809 3809
Size: 5897
BsrFI r'CCGG_y
Cuts at: 3895 4076 3895
Size: 181 5716
BssSI C'ACGA_G
Cuts at: 4168 4876 4168
Size: 708 5189
BstAPI GCAn_nnn'nTGC
Cuts at: 2169 3274 2169
Size: 1105 4792
BstBI TT'CG_AA
Cuts at: 4258 4258
Size: 5897
BstYI r'GATC_y
Cuts at: 1 1095 1939 2295 2922 3389 3412 3747 3993
Size: 1094 844 356 627 467 23 335 246
Cuts at: 3993 4299 4311 4397 5449 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1094 1052 844 627 467 449 356 335
306 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1351 1351
Size: 5897
Bsu36I CC'TnA_GG
Cuts at: 194 194
Size: 5897
BtgI C'CryG_G
Cuts at: 158 1651 158
Size: 1493 4404
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3847 4028 4191 3847
Size: 181 163 5553
Fragments arranged by size:
5553 181 163
BtsI GCAGTG_nn'
Cuts at: 1907 3140 1907
Size: 1233 4664
DraI TTT'AAA
Cuts at: 1274 1386 1676 2896 1274
Size: 112 290 1220 4275
Fragments arranged by size:
4275 1220 290 112
DraIII CAC_nnn'GTG
Cuts at: 2104 2104
Size: 5897
DrdI GACnn_nn'nnGTC
Cuts at: 3603 4947 3603
Size: 1344 4553
EaeI y'GGCC_r
Cuts at: 134 1592 3482 3656 4047 4074 5419 134
Size: 1458 1890 174 391 27 1345 612
Fragments arranged by size:
1890 1458 1345 612 391 174 27
EagI C'GGCC_G
Cuts at: 134 3482 134
Size: 3348 2549
EarI CTCTTCn'nnn_
Cuts at: 595 1139 1193 2339 2530 2879 3920 4130 595
Size: 544 54 1146 191 349 1041 210 2362
Fragments arranged by size:
2362 1146 1041 544 349 210 191 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4105 4847 4993 5707 4105
Size: 742 146 714 4295
Fragments arranged by size:
4295 742 714 146
EcoICRI GAG'CTC
Cuts at: 2909 2909
Size: 5897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 576 1120 1211 1280 1879 2528 2554 2717 3722
Size: 544 91 69 599 649 26 163 1005
Cuts at: 3722 4154 4507 5169 576
Size: 432 353 662 1304
Fragments arranged by size:
1304 1005 662 649 599 544 432 353
163 91 69 26
EcoO109I rG'GnC_Cy
Cuts at: 201 228 2432 3036 201
Size: 27 2204 604 3062
Fragments arranged by size:
3062 2204 604 27
EcoRI G'AATT_C
Cuts at: 1008 2901 1008
Size: 1893 4004
EcoRV GAT'ATC
Cuts at: 650 650
Size: 5897
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3916 3916
Size: 5897
FspI TGC'GCA
Cuts at: 3678 3678
Size: 5897
HaeII r_GCGC'y
Cuts at: 3331 3579 4809 5580 3331
Size: 248 1230 771 3648
Fragments arranged by size:
3648 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 294 326 430 462 2501 2533 2582 2614 2811
Size: 32 104 32 2039 32 49 32 197
Cuts at: 2811 2843 3739 3769 3771 3801 294
Size: 32 896 30 2 30 2390
Fragments arranged by size:
2390 2039 896 197 104 49 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 335 432 2936 335
Size: 97 2504 3296
Fragments arranged by size:
3296 2504 97
HpaI GTT'AAC
Cuts at: 335 335
Size: 5897
KasI G'GCGC_C
Cuts at: 3327 3575 3327
Size: 248 5649
KpnI G_GTAC'C
Cuts at: 2917 2917
Size: 5897
MfeI C'AATT_G
Cuts at: 2609 2609
Size: 5897
MluI A'CGCG_T
Cuts at: 1966 5330 1966
Size: 3364 2533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1278 4656 4840 1278
Size: 3378 184 2335
Fragments arranged by size:
3378 2335 184
MscI TGG'CCA
Cuts at: 1594 3658 1594
Size: 2064 3833
MslI CAynn'nnrTG
Cuts at: 3886 4013 3886
Size: 127 5770
MspA1I CmG'CkG
Cuts at: 151 160 218 832 3008 3322 3682 4466 4711
Size: 9 58 614 2176 314 360 784 245
Cuts at: 4711 151
Size: 1337
Fragments arranged by size:
2176 1337 784 614 360 314 245 58
9
NaeI GCC'GGC
Cuts at: 4078 4078
Size: 5897
NarI GG'CG_CC
Cuts at: 3328 3576 3328
Size: 248 5649
NcoI C'CATG_G
Cuts at: 1651 1651
Size: 5897
NdeI CA'TA_TG
Cuts at: 1556 2239 1556
Size: 683 5214
NgoMIV G'CCGG_C
Cuts at: 4076 4076
Size: 5897
NheI G'CTAG_C
Cuts at: 2076 2076
Size: 5897
NspI r_CATG'y
Cuts at: 614 2950 3450 5053 614
Size: 2336 500 1603 1458
Fragments arranged by size:
2336 1603 1458 500
PciI A'CATG_T
Cuts at: 610 5049 610
Size: 4439 1458
PfoI T'CCnGG_A
Cuts at: 2505 2505
Size: 5897
PmeI GTTT'AAAC
Cuts at: 2896 2896
Size: 5897
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 433 465 4343 4375 433
Size: 32 3878 32 1955
Fragments arranged by size:
3878 1955 32 32
PpuMI rG'GwC_Cy
Cuts at: 201 228 201
Size: 27 5870
PsiI TTA'TAA
Cuts at: 2060 2060
Size: 5897
PstI C_TGCA'G
Cuts at: 1914 2174 2944 3629 1914
Size: 260 770 685 4182
Fragments arranged by size:
4182 770 685 260
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5897
PvuII CAG'CTG
Cuts at: 832 3322 3682 832
Size: 2490 360 3047
Fragments arranged by size:
3047 2490 360
RsrII CG'GwC_CG
Cuts at: 4092 4092
Size: 5897
SacI G_AGCT'C
Cuts at: 2911 2911
Size: 5897
SacII CC_GC'GG
Cuts at: 161 161
Size: 5897
SalI G'TCGA_C
Cuts at: 2934 2934
Size: 5897
SapI GCTCTTCn'nnn_
Cuts at: 3920 4130 3920
Size: 210 5687
SbfI CC_TGCA'GG
Cuts at: 2944 2944
Size: 5897
ScaI AGT'ACT
Cuts at: 5414 5414
Size: 5897
SfcI C'TryA_G
Cuts at: 33 466 1148 1910 2170 2940 3625 4593 4784
Size: 433 682 762 260 770 685 968 191
Cuts at: 4784 33
Size: 1146
Fragments arranged by size:
1146 968 770 762 685 682 433 260
191
SfoI GGC'GCC
Cuts at: 3329 3577 3329
Size: 248 5649
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5897
SmaI CCC'GGG
Cuts at: 2919 2919
Size: 5897
SmlI C'TyrA_G
Cuts at: 247 1579 3052 3967 4681 4943 247
Size: 1332 1473 915 714 262 1201
Fragments arranged by size:
1473 1332 1201 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5897
SphI G_CATG'C
Cuts at: 2950 3450 2950
Size: 500 5397
SspI AAT'ATT
Cuts at: 2409 2409
Size: 5897
StyI C'CwwG_G
Cuts at: 1651 3031 3385 1651
Size: 1380 354 4163
Fragments arranged by size:
4163 1380 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 879 1856 2141 2370 3480 3613 4072 4281 879
Size: 977 285 229 1110 133 459 209 2495
Fragments arranged by size:
2495 1110 977 459 285 229 209 133
TatI w'GTAC_w
Cuts at: 1609 5412 1609
Size: 3803 2094
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1320 1352 5414 5446 1320
Size: 32 4062 32 1771
Fragments arranged by size:
4062 1771 32 32
Tth111I GACn'n_nGTC
Cuts at: 3694 3694
Size: 5897
XbaI T'CTAG_A
Cuts at: 1098 2928 1098
Size: 1830 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3392 3392
Size: 5897
XmaI C'CCGG_G
Cuts at: 2917 2917
Size: 5897
XmnI GAAnn'nnTTC
Cuts at: 2111 2111
Size: 5897
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PciI PfoI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BfrBI BglI BmgBI
BsaXI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI
ClaI CspCI EcoNI FseI FspAI HindIII NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI