(Circular) (Six-base) MAPSORT of: pF1KB3027.seq Check: 9624 from: 1 to: 6755 pF1KB3027 6755 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1270 1805 2453 1270 Size: 535 648 5572 Fragments arranged by size: 5572 648 535 AccI GT'mk_AC Cuts at: 200 3068 3793 200 Size: 2868 725 3162 Fragments arranged by size: 3162 2868 725 Acc65I G'GTAC_C Cuts at: 2091 3771 2091 Size: 1680 5075 AclI AA'CG_TT Cuts at: 6595 6595 Size: 6755 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1765 2049 2414 3012 3128 4580 5012 5759 6027 Size: 284 365 598 116 1452 432 747 268 Cuts at: 6027 1765 Size: 2493 Fragments arranged by size: 2493 1452 747 598 432 365 284 268 116 AfeI AGC'GCT Cuts at: 3138 3138 Size: 6755 AflIII A'CryG_T Cuts at: 989 2738 4107 5211 6188 989 Size: 1749 1369 1104 977 1556 Fragments arranged by size: 1749 1556 1369 1104 977 AgeI A'CCGG_T Cuts at: 704 5143 704 Size: 4439 2316 AhdI GACnn_n'nnGTC Cuts at: 3111 3111 Size: 6755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2300 3727 2300 Size: 1427 5328 AlwNI CAG_nnn'CTG Cuts at: 1053 1633 1725 1736 2216 3109 3244 3576 4060 Size: 580 92 11 480 893 135 332 484 Cuts at: 4060 5627 1053 Size: 1567 2181 Fragments arranged by size: 2181 1567 893 580 484 480 332 135 92 11 ApaI G_GGCC'C Cuts at: 1155 1857 2897 1155 Size: 702 1040 5013 Fragments arranged by size: 5013 1040 702 ApaLI G'TGCA_C Cuts at: 1006 5525 1006 Size: 4519 2236 ApoI r'AATT_y Cuts at: 2172 3759 6108 6364 2172 Size: 1587 2349 256 2563 Fragments arranged by size: 2563 2349 1587 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6755 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6755 AvaI C'yCGr_G Cuts at: 484 2625 3200 3434 3521 3775 484 Size: 2141 575 234 87 254 3464 Fragments arranged by size: 3464 2141 575 254 234 87 BamHI G'GATC_C Cuts at: 1175 3780 1175 Size: 2605 4150 BanI G'GyrC_C Cuts at: 302 1120 1396 1405 2091 2306 3771 4185 4433 Size: 818 276 9 686 215 1465 414 248 Cuts at: 4433 4468 302 Size: 35 2589 Fragments arranged by size: 2589 1465 818 686 414 276 248 215 35 9 BanII G_rGCy'C Cuts at: 1155 1288 1546 1857 1911 2897 2950 2968 3693 Size: 133 258 311 54 986 53 18 725 Cuts at: 3693 3769 4799 1155 Size: 76 1030 3111 Fragments arranged by size: 3111 1030 986 725 311 258 133 76 54 53 18 BbeI G_GCGC'C Cuts at: 306 1400 2310 4189 4437 306 Size: 1094 910 1879 248 2624 Fragments arranged by size: 2624 1879 1094 910 248 BbsI GAAGACnn'nnnn_ Cuts at: 136 811 1197 2812 3002 136 Size: 675 386 1615 190 3889 Fragments arranged by size: 3889 1615 675 386 190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 703 737 889 923 2479 2513 703 Size: 34 152 34 1556 34 4945 Fragments arranged by size: 4945 1556 152 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3225 4653 5414 6755 3225 Size: 1428 761 1341 3225 Fragments arranged by size: 3225 1428 1341 761 BclI T'GATC_A Cuts at: 2540 3413 4275 2540 Size: 873 862 5020 Fragments arranged by size: 5020 873 862 BfrBI ATG'CAT Cuts at: 2349 2349 Size: 6755 BglI GCCn_nnn'nGGC Cuts at: 1354 2826 2835 2879 1354 Size: 1472 9 44 5230 Fragments arranged by size: 5230 1472 44 9 BglII A'GATC_T Cuts at: 836 4270 6307 836 Size: 3434 2037 1284 Fragments arranged by size: 3434 2037 1284 BlpI GC'TnA_GC Cuts at: 3867 3867 Size: 6755 Bme1580I G_kGCm'C Cuts at: 178 1010 1101 1108 1155 1649 1857 2897 3208 Size: 832 91 7 47 494 208 1040 311 Cuts at: 3208 3271 3476 4380 4473 5529 178 Size: 63 205 904 93 1056 1404 Fragments arranged by size: 1404 1056 1040 904 832 494 311 208 205 93 91 63 47 7 BmgBI CAC'GTC Cuts at: 2599 2599 Size: 6755 BmrI ACTGGGnnnn_n' Cuts at: 1860 2951 3967 4048 4383 6527 1860 Size: 1091 1016 81 335 2144 2088 Fragments arranged by size: 2144 2088 1091 1016 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 641 3252 3276 3547 5122 641 Size: 2611 24 271 1575 2274 Fragments arranged by size: 2611 2274 1575 271 24 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 883 1439 1560 2390 2449 2497 3502 3658 883 Size: 556 121 830 59 48 1005 156 3980 Fragments arranged by size: 3980 1005 830 556 156 121 59 48 Bpu10I CC'TnA_GC Cuts at: 1287 2609 1287 Size: 1322 5433 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1855 2206 3931 4810 5302 5600 1855 Size: 351 1725 879 492 298 3010 Fragments arranged by size: 3010 1725 879 492 351 298 BsaI GGTCTCn'nnnn_ Cuts at: 178 354 3727 6459 178 Size: 176 3373 2732 474 Fragments arranged by size: 3373 2732 474 176 BsaAI yAC'GTr Cuts at: 4108 4108 Size: 6755 BsaBI GATnn'nnATC Cuts at: 6 4269 6306 6 Size: 4263 2037 455 Fragments arranged by size: 4263 2037 455 BsaHI Gr'CG_yC Cuts at: 303 1397 2307 4186 4434 5136 6632 303 Size: 1094 910 1879 248 702 1496 426 Fragments arranged by size: 1879 1496 1094 910 702 426 248 BsaWI w'CCGG_w Cuts at: 704 3149 3944 4465 5143 5417 5564 5975 704 Size: 2445 795 521 678 274 147 411 1484 Fragments arranged by size: 2445 1484 795 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 856 886 856 Size: 30 6725 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 502 742 778 1516 1531 1534 2203 2710 502 Size: 240 36 738 15 3 669 507 4547 Fragments arranged by size: 4547 738 669 507 240 36 15 3 BseYI C'CCAG_C Cuts at: 315 334 392 1148 1349 2869 2900 3405 4180 Size: 19 58 756 201 1520 31 505 775 Cuts at: 4180 4938 5515 6011 315 Size: 758 577 496 1059 Fragments arranged by size: 1520 1059 775 758 756 577 505 496 201 58 31 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1711 1711 Size: 6755 BsiEI CG_ry'CG Cuts at: 104 4297 4343 5551 104 Size: 4193 46 1208 1308 Fragments arranged by size: 4193 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 622 1010 1235 1546 1911 2294 2968 3769 4547 Size: 388 225 311 365 383 674 801 778 Cuts at: 4547 4737 5529 622 Size: 190 792 1848 Fragments arranged by size: 1848 801 792 778 674 388 383 365 311 225 190 BsmI GAATG_Cn' Cuts at: 2502 2502 Size: 6755 BsmBI CGTCTCn'nnnn_ Cuts at: 1951 2332 2584 1951 Size: 381 252 6122 Fragments arranged by size: 6122 381 252 Bsp1286I G_dGCh'C Cuts at: 178 622 1010 1101 1108 1155 1235 1288 1546 Size: 444 388 91 7 47 80 53 258 Cuts at: 1546 1649 1857 1911 2294 2897 2950 2968 3208 Size: 103 208 54 383 603 53 18 240 Cuts at: 3208 3271 3476 3693 3769 4380 4473 4547 4737 Size: 63 205 217 76 611 93 74 190 Cuts at: 4737 4799 5529 178 Size: 62 730 1404 Fragments arranged by size: 1404 730 611 603 444 388 383 258 240 217 208 205 190 103 93 91 80 76 74 63 62 54 53 53 47 18 7 BspEI T'CCGG_A Cuts at: 5975 5975 Size: 6755 BspHI T'CATG_A Cuts at: 788 2912 5931 788 Size: 2124 3019 1612 Fragments arranged by size: 3019 2124 1612 BspMI ACCTGCnnnn'nnnn_ Cuts at: 196 1270 1805 2453 3805 4019 4321 4702 196 Size: 1074 535 648 1352 214 302 381 2249 Fragments arranged by size: 2249 1352 1074 648 535 381 302 214 BsrBI CCG'CTC Cuts at: 50 2415 2481 2522 2613 5047 5101 6585 6750 Size: 2365 66 41 91 2434 54 1484 165 Cuts at: 6750 50 Size: 55 Fragments arranged by size: 2434 2365 1484 165 91 66 55 54 41 BsrDI GCAATG_nn' Cuts at: 951 975 1771 4667 951 Size: 24 796 2896 3039 Fragments arranged by size: 3039 2896 796 24 BsrFI r'CCGG_y Cuts at: 704 2073 2834 4753 4934 5143 704 Size: 1369 761 1919 181 209 2316 Fragments arranged by size: 2316 1919 1369 761 209 181 BssSI C'ACGA_G Cuts at: 1082 1918 3370 5026 5384 1082 Size: 836 1452 1656 358 2453 Fragments arranged by size: 2453 1656 1452 836 358 BstAPI GCAn_nnn'nTGC Cuts at: 1304 1725 3576 4132 1304 Size: 421 1851 556 3927 Fragments arranged by size: 3927 1851 556 421 BstBI TT'CG_AA Cuts at: 5116 5116 Size: 6755 BstXI CCAn_nnnn'nTGG Cuts at: 114 1250 1357 114 Size: 1136 107 5512 Fragments arranged by size: 5512 1136 107 BstYI r'GATC_y Cuts at: 1 836 1175 1604 3780 4247 4270 4605 4851 Size: 835 339 429 2176 467 23 335 246 Cuts at: 4851 5863 5949 5961 6307 1 Size: 1012 86 12 346 449 Fragments arranged by size: 2176 1012 835 467 449 429 346 339 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 3069 3069 Size: 6755 Bsu36I CC'TnA_GG Cuts at: 1040 1181 3237 1040 Size: 141 2056 4558 Fragments arranged by size: 4558 2056 141 BtgI C'CryG_G Cuts at: 107 1982 2123 2874 3263 3276 3453 107 Size: 1875 141 751 389 13 177 3409 Fragments arranged by size: 3409 1875 751 389 177 141 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4705 4886 5049 4705 Size: 181 163 6411 Fragments arranged by size: 6411 181 163 BtsI GCAGTG_nn' Cuts at: 2267 2560 3231 3577 3735 3998 2267 Size: 293 671 346 158 263 5024 Fragments arranged by size: 5024 671 346 293 263 158 DraI TTT'AAA Cuts at: 3754 3754 Size: 6755 DraIII CAC_nnn'GTG Cuts at: 2530 2530 Size: 6755 DrdI GACnn_nn'nnGTC Cuts at: 4461 5319 4461 Size: 858 5897 EaeI y'GGCC_r Cuts at: 420 688 1001 3273 4340 4514 4905 4932 6277 Size: 268 313 2272 1067 174 391 27 1345 Cuts at: 6277 420 Size: 898 Fragments arranged by size: 2272 1345 1067 898 391 313 268 174 27 EagI C'GGCC_G Cuts at: 4340 4340 Size: 6755 EarI CTCTTCn'nnn_ Cuts at: 985 1699 2405 3049 3298 3550 4778 4988 985 Size: 714 706 644 249 252 1228 210 2752 Fragments arranged by size: 2752 1228 714 706 644 252 249 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2284 2583 4963 5273 5419 6565 2284 Size: 299 2380 310 146 1146 2474 Fragments arranged by size: 2474 2380 1146 310 299 146 EcoICRI GAG'CTC Cuts at: 1544 1909 2966 3767 1544 Size: 365 1057 801 4532 Fragments arranged by size: 4532 1057 801 365 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 883 1439 1560 1765 2049 2390 2414 2449 2497 Size: 556 121 205 284 341 24 35 48 Cuts at: 2497 3012 3128 3502 3658 4580 5012 5759 6027 Size: 515 116 374 156 922 432 747 268 Cuts at: 6027 883 Size: 1611 Fragments arranged by size: 1611 922 747 556 515 432 374 341 284 268 205 156 121 116 48 35 24 EcoNI CCTnn'n_nnAGG Cuts at: 3622 3684 3622 Size: 62 6693 EcoO109I rG'GnC_Cy Cuts at: 492 1292 1767 1854 2731 2893 2894 3615 3894 Size: 800 475 87 877 162 1 721 279 Cuts at: 3894 492 Size: 3353 Fragments arranged by size: 3353 877 800 721 475 279 162 87 1 EcoRI G'AATT_C Cuts at: 3759 3759 Size: 6755 EcoRV GAT'ATC Cuts at: 786 786 Size: 6755 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2166 3078 4774 2166 Size: 912 1696 4147 Fragments arranged by size: 4147 1696 912 FseI GG_CCGG'CC Cuts at: 2838 2838 Size: 6755 FspI TGC'GCA Cuts at: 4536 4536 Size: 6755 HaeII r_GCGC'y Cuts at: 306 1149 1400 2310 3140 4189 4437 5459 6438 Size: 843 251 910 830 1049 248 1022 979 Cuts at: 6438 306 Size: 623 Fragments arranged by size: 1049 1022 979 910 843 830 623 251 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 448 480 856 888 3097 3129 3331 3363 3405 Size: 32 376 32 2209 32 202 32 42 Cuts at: 3405 3437 4597 4627 4629 4659 448 Size: 32 1160 30 2 30 2544 Fragments arranged by size: 2544 2209 1160 376 202 42 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 3794 3794 Size: 6755 HindIII A'AGCT_T Cuts at: 2572 2572 Size: 6755 KasI G'GCGC_C Cuts at: 302 1396 2306 4185 4433 302 Size: 1094 910 1879 248 2624 Fragments arranged by size: 2624 1879 1094 910 248 KpnI G_GTAC'C Cuts at: 2095 3775 2095 Size: 1680 5075 MluI A'CGCG_T Cuts at: 6188 6188 Size: 6755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 611 781 3677 5426 5610 611 Size: 170 2896 1749 184 1756 Fragments arranged by size: 2896 1756 1749 184 170 MscI TGG'CCA Cuts at: 4516 4516 Size: 6755 MslI CAynn'nnrTG Cuts at: 112 171 605 1372 1987 3469 4744 4871 112 Size: 59 434 767 615 1482 1275 127 1996 Fragments arranged by size: 1996 1482 1275 767 615 434 127 59 MspA1I CmG'CkG Cuts at: 535 1884 1995 2812 3032 3278 3354 3866 4180 Size: 1349 111 817 220 246 76 512 314 Cuts at: 4180 4540 5553 5798 535 Size: 360 1013 245 1492 Fragments arranged by size: 1492 1349 1013 817 512 360 314 246 245 220 111 76 NaeI GCC'GGC Cuts at: 2836 4936 2836 Size: 2100 4655 NarI GG'CG_CC Cuts at: 303 1397 2307 4186 4434 303 Size: 1094 910 1879 248 2624 Fragments arranged by size: 2624 1879 1094 910 248 NcoI C'CATG_G Cuts at: 107 1982 2123 3453 107 Size: 1875 141 1330 3409 Fragments arranged by size: 3409 1875 1330 141 NgoMIV G'CCGG_C Cuts at: 2834 4934 2834 Size: 2100 4655 NsiI A_TGCA'T Cuts at: 2351 2351 Size: 6755 NspI r_CATG'y Cuts at: 993 1346 2349 2742 3808 4308 5215 993 Size: 353 1003 393 1066 500 907 2533 Fragments arranged by size: 2533 1066 1003 907 500 393 353 PciI A'CATG_T Cuts at: 989 2738 5211 989 Size: 1749 2473 2533 Fragments arranged by size: 2533 2473 1749 PflMI CCAn_nnn'nTGG Cuts at: 399 2364 3022 3294 399 Size: 1965 658 272 3860 Fragments arranged by size: 3860 1965 658 272 PfoI T'CCnGG_A Cuts at: 1576 1576 Size: 6755 PmeI GTTT'AAAC Cuts at: 3754 3754 Size: 6755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5894 5926 5894 Size: 32 6723 PpuMI rG'GwC_Cy Cuts at: 1767 2731 3615 1767 Size: 964 884 4907 Fragments arranged by size: 4907 964 884 PspOMI G'GGCC_C Cuts at: 1151 1853 2893 1151 Size: 702 1040 5013 Fragments arranged by size: 5013 1040 702 PspXI vC'TCGA_Gb Cuts at: 2625 2625 Size: 6755 PstI C_TGCA'G Cuts at: 726 1073 1790 3085 3698 3802 4487 726 Size: 347 717 1295 613 104 685 2994 Fragments arranged by size: 2994 1295 717 685 613 347 104 PvuI CG_AT'CG Cuts at: 104 104 Size: 6755 PvuII CAG'CTG Cuts at: 535 1995 2812 4180 4540 535 Size: 1460 817 1368 360 2750 Fragments arranged by size: 2750 1460 1368 817 360 RsrII CG'GwC_CG Cuts at: 4950 4950 Size: 6755 SacI G_AGCT'C Cuts at: 1546 1911 2968 3769 1546 Size: 365 1057 801 4532 Fragments arranged by size: 4532 1057 801 365 SacII CC_GC'GG Cuts at: 3279 3279 Size: 6755 SalI G'TCGA_C Cuts at: 3792 3792 Size: 6755 SanDI GG'GwC_CC Cuts at: 1767 2731 1767 Size: 964 5791 SapI GCTCTTCn'nnn_ Cuts at: 1699 2405 4778 4988 1699 Size: 706 2373 210 3466 Fragments arranged by size: 3466 2373 706 210 SbfI CC_TGCA'GG Cuts at: 3802 3802 Size: 6755 ScaI AGT'ACT Cuts at: 6272 6272 Size: 6755 SfcI C'TryA_G Cuts at: 33 722 1069 1786 3081 3694 3798 4483 5476 Size: 689 347 717 1295 613 104 685 993 Cuts at: 5476 5667 33 Size: 191 1121 Fragments arranged by size: 1295 1121 993 717 689 685 613 347 191 104 SfiI GGCCn_nnn'nGGCC Cuts at: 2835 2835 Size: 6755 SfoI GGC'GCC Cuts at: 304 1398 2308 4187 4435 304 Size: 1094 910 1879 248 2624 Fragments arranged by size: 2624 1879 1094 910 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6755 SmaI CCC'GGG Cuts at: 3777 3777 Size: 6755 SmlI C'TyrA_G Cuts at: 1870 2221 2625 3910 4825 5317 5579 1870 Size: 351 404 1285 915 492 262 3046 Fragments arranged by size: 3046 1285 915 492 404 351 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6755 SphI G_CATG'C Cuts at: 2349 3808 4308 2349 Size: 1459 500 4796 Fragments arranged by size: 4796 1459 500 StuI AGG'CCT Cuts at: 2726 3256 2726 Size: 530 6225 StyI C'CwwG_G Cuts at: 107 1565 1982 2027 2123 2234 3453 3889 4243 Size: 1458 417 45 96 111 1219 436 354 Cuts at: 4243 107 Size: 2619 Fragments arranged by size: 2619 1458 1219 436 417 354 111 96 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 348 872 3592 4338 4471 4930 5139 348 Size: 524 2720 746 133 459 209 1964 Fragments arranged by size: 2720 1964 746 524 459 209 133 TatI w'GTAC_w Cuts at: 3669 6270 3669 Size: 2601 4154 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1589 1621 3358 3390 6272 6304 1589 Size: 32 1737 32 2882 32 2040 Fragments arranged by size: 2882 2040 1737 32 32 32 Tth111I GACn'n_nGTC Cuts at: 4552 4552 Size: 6755 XbaI T'CTAG_A Cuts at: 3786 3786 Size: 6755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1247 2365 3588 4250 1247 Size: 1118 1223 662 3752 Fragments arranged by size: 3752 1223 1118 662 XhoI C'TCGA_G Cuts at: 2625 2625 Size: 6755 XmaI C'CCGG_G Cuts at: 3775 3775 Size: 6755 XmnI GAAnn'nnTTC Cuts at: 133 3058 133 Size: 2925 3830 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AflII AleI AloI AscI AvrII BaeI BbvCI BmtI BsiWI BsrGI BssHII BstEII ClaI CspCI FspAI HpaI MfeI NdeI NheI NotI NruI PacI PasI PmlI PshAI PsiI PsrI SexAI SgrAI SnaBI SrfI SspI SwaI ZraI