Restriction map for
pF1KB3028
(Circular) (Six-base) MAPSORT of: pF1KB3028.seq Check: 4206 from: 1 to: 6665
pF1KB3028 6665 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1877 2452 3641 1877
Size: 575 1189 4901
Fragments arranged by size:
4901 1189 575
AccI GT'mk_AC
Cuts at: 1029 3703 1029
Size: 2674 3991
Acc65I G'GTAC_C
Cuts at: 784 3681 784
Size: 2897 3768
AclI AA'CG_TT
Cuts at: 6505 6505
Size: 6665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 975 1290 2767 3191 4490 4922 5669 5937 975
Size: 315 1477 424 1299 432 747 268 1703
Fragments arranged by size:
1703 1477 1299 747 432 424 315 268
AfeI AGC'GCT
Cuts at: 3478 3478
Size: 6665
AflIII A'CryG_T
Cuts at: 1337 4017 5121 6098 1337
Size: 2680 1104 977 1904
Fragments arranged by size:
2680 1904 1104 977
AgeI A'CCGG_T
Cuts at: 5053 5053
Size: 6665
AleI CACnn'nnGTG
Cuts at: 2200 2200
Size: 6665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2269 2693 3601 2269
Size: 424 908 5333
Fragments arranged by size:
5333 908 424
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 931 963 931
Size: 32 6633
AlwNI CAG_nnn'CTG
Cuts at: 264 531 919 2901 3003 3970 5537 264
Size: 267 388 1982 102 967 1567 1392
Fragments arranged by size:
1982 1567 1392 967 388 267 102
ApaI G_GGCC'C
Cuts at: 670 1906 2891 670
Size: 1236 985 4444
Fragments arranged by size:
4444 1236 985
ApaLI G'TGCA_C
Cuts at: 5435 5435
Size: 6665
ApoI r'AATT_y
Cuts at: 3669 6018 6274 3669
Size: 2349 256 4060
Fragments arranged by size:
4060 2349 256
AseI AT'TA_AT
Cuts at: 20 1580 20
Size: 1560 5105
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6665
AvaI C'yCGr_G
Cuts at: 1989 2168 2684 2704 3685 1989
Size: 179 516 20 981 4969
Fragments arranged by size:
4969 981 516 179 20
BamHI G'GATC_C
Cuts at: 411 566 3165 3690 411
Size: 155 2599 525 3386
Fragments arranged by size:
3386 2599 525 155
BanI G'GyrC_C
Cuts at: 784 1875 3390 3653 3681 4095 4343 4378 784
Size: 1091 1515 263 28 414 248 35 3071
Fragments arranged by size:
3071 1515 1091 414 263 248 35 28
BanII G_rGCy'C
Cuts at: 585 670 1906 2009 2175 2711 2891 2963 3406
Size: 85 1236 103 166 536 180 72 443
Cuts at: 3406 3451 3679 4709 585
Size: 45 228 1030 2541
Fragments arranged by size:
2541 1236 1030 536 443 228 180 166
103 85 72 45
BbeI G_GCGC'C
Cuts at: 4099 4347 4099
Size: 248 6417
BciVI GTATCCnnnnn_n'
Cuts at: 4563 5324 6665 4563
Size: 761 1341 4563
Fragments arranged by size:
4563 1341 761
BclI T'GATC_A
Cuts at: 4185 4185
Size: 6665
BglI GCCn_nnn'nGGC
Cuts at: 1910 2400 3280 3410 3508 1910
Size: 490 880 130 98 5067
Fragments arranged by size:
5067 880 490 130 98
BglII A'GATC_T
Cuts at: 4180 6217 4180
Size: 2037 4628
BlpI GC'TnA_GC
Cuts at: 364 1285 1444 1816 3256 3400 3777 364
Size: 921 159 372 1440 144 377 3252
Fragments arranged by size:
3252 1440 921 377 372 159 144
Bme1580I G_kGCm'C
Cuts at: 670 1880 1906 1915 2891 4290 4383 5439 670
Size: 1210 26 9 976 1399 93 1056 1896
Fragments arranged by size:
1896 1399 1210 1056 976 93 26 9
BmgBI CAC'GTC
Cuts at: 1167 1167
Size: 6665
BmrI ACTGGGnnnn_n'
Cuts at: 274 629 1609 3877 3958 4293 6437 274
Size: 355 980 2268 81 335 2144 502
Fragments arranged by size:
2268 2144 980 502 355 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 567 569 2026 3198 3465 3519 5032 567
Size: 2 1457 1172 267 54 1513 2200
Fragments arranged by size:
2200 1513 1457 1172 267 54 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 956 1402 2146 2801 3146 3465 3478 956
Size: 446 744 655 345 319 13 4143
Fragments arranged by size:
4143 744 655 446 345 319 13
Bpu10I CC'TnA_GC
Cuts at: 2449 2449
Size: 6665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2262 3841 4720 5212 5510 2262
Size: 1579 879 492 298 3417
Fragments arranged by size:
3417 1579 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 670 2777 6369 670
Size: 2107 3592 966
Fragments arranged by size:
3592 2107 966
BsaAI yAC'GTr
Cuts at: 4018 4018
Size: 6665
BsaBI GATnn'nnATC
Cuts at: 6 4179 6216 6
Size: 4173 2037 455
Fragments arranged by size:
4173 2037 455
BsaHI Gr'CG_yC
Cuts at: 4096 4344 5046 6542 4096
Size: 248 702 1496 4219
Fragments arranged by size:
4219 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3854 4375 5053 5327 5474 5885 3854
Size: 521 678 274 147 411 4634
Fragments arranged by size:
4634 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 615 645 931 961 2359 2389 615
Size: 30 286 30 1398 30 4891
Fragments arranged by size:
4891 1398 286 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 2814 2966 2971 3367 289
Size: 2525 152 5 396 3587
Fragments arranged by size:
3587 2525 396 152 5
BseYI C'CCAG_C
Cuts at: 132 292 1104 1181 1296 1905 2528 2711 2996
Size: 160 812 77 115 609 623 183 285
Cuts at: 2996 3229 4090 4848 5425 5921 132
Size: 233 861 758 577 496 876
Fragments arranged by size:
876 861 812 758 623 609 577 496
285 233 183 160 115 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 863 3587 598
Size: 265 2724 3676
Fragments arranged by size:
3676 2724 265
BsiEI CG_ry'CG
Cuts at: 104 4207 4253 5461 104
Size: 4103 46 1208 1308
Fragments arranged by size:
4103 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 585 644 1817 2450 2610 2963 3451 3679 4457
Size: 59 1173 633 160 353 488 228 778
Cuts at: 4457 4647 5439 585
Size: 190 792 1811
Fragments arranged by size:
1811 1173 792 778 633 488 353 228
190 160 59
BsmI GAATG_Cn'
Cuts at: 1448 1857 2682 1448
Size: 409 825 5431
Fragments arranged by size:
5431 825 409
BsmBI CGTCTCn'nnnn_
Cuts at: 2832 2832
Size: 6665
Bsp1286I G_dGCh'C
Cuts at: 585 644 670 1817 1880 1906 1915 2009 2175
Size: 59 26 1147 63 26 9 94 166
Cuts at: 2175 2450 2610 2711 2891 2963 3406 3451 3679
Size: 275 160 101 180 72 443 45 228
Cuts at: 3679 4290 4383 4457 4647 4709 5439 585
Size: 611 93 74 190 62 730 1811
Fragments arranged by size:
1811 1147 730 611 443 275 228 190
180 166 160 101 94 93 74 72
63 62 59 45 26 26 9
BspEI T'CCGG_A
Cuts at: 5885 5885
Size: 6665
BspHI T'CATG_A
Cuts at: 1560 5841 1560
Size: 4281 2384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1487 1877 2452 3641 3715 3929 4231 4612 1487
Size: 390 575 1189 74 214 302 381 3540
Fragments arranged by size:
3540 1189 575 390 381 302 214 74
BsrBI CCG'CTC
Cuts at: 50 2943 4957 5011 6495 6660 50
Size: 2893 2014 54 1484 165 55
Fragments arranged by size:
2893 2014 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1110 2031 4577 1110
Size: 921 2546 3198
Fragments arranged by size:
3198 2546 921
BsrFI r'CCGG_y
Cuts at: 1693 2610 3251 4663 4844 5053 1693
Size: 917 641 1412 181 209 3305
Fragments arranged by size:
3305 1412 917 641 209 181
BsrGI T'GTAC_A
Cuts at: 2573 2573
Size: 6665
BssSI C'ACGA_G
Cuts at: 2437 3414 4936 5294 2437
Size: 977 1522 358 3808
Fragments arranged by size:
3808 1522 977 358
BstAPI GCAn_nnn'nTGC
Cuts at: 120 129 1737 1981 3653 4042 120
Size: 9 1608 244 1672 389 2743
Fragments arranged by size:
2743 1672 1608 389 244 9
BstBI TT'CG_AA
Cuts at: 5026 5026
Size: 6665
BstEII G'GTnAC_C
Cuts at: 186 186
Size: 6665
BstXI CCAn_nnnn'nTGG
Cuts at: 342 1899 342
Size: 1557 5108
BstYI r'GATC_y
Cuts at: 1 411 473 566 2897 3165 3690 4157 4180
Size: 410 62 93 2331 268 525 467 23
Cuts at: 4180 4515 4761 5773 5859 5871 6217 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2331 1012 525 467 449 410 346 335
268 246 93 86 62 23 12
Bsu36I CC'TnA_GG
Cuts at: 323 2212 3573 323
Size: 1889 1361 3415
Fragments arranged by size:
3415 1889 1361
BtgI C'CryG_G
Cuts at: 505 2951 3347 3491 505
Size: 2446 396 144 3679
Fragments arranged by size:
3679 2446 396 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1835 2527 4615 4796 4959 1835
Size: 692 2088 181 163 3541
Fragments arranged by size:
3541 2088 692 181 163
BtsI GCAGTG_nn'
Cuts at: 1275 1378 1658 2799 3451 3908 1275
Size: 103 280 1141 652 457 4032
Fragments arranged by size:
4032 1141 652 457 280 103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1952 1987 1952
Size: 35 6630
DraI TTT'AAA
Cuts at: 3664 3664
Size: 6665
DraIII CAC_nnn'GTG
Cuts at: 2439 2439
Size: 6665
DrdI GACnn_nn'nnGTC
Cuts at: 4371 5229 4371
Size: 858 5807
EaeI y'GGCC_r
Cuts at: 1691 1931 1948 3065 3422 3557 4250 4424 4815
Size: 240 17 1117 357 135 693 174 391
Cuts at: 4815 4842 6187 1691
Size: 27 1345 2169
Fragments arranged by size:
2169 1345 1117 693 391 357 240 174
135 27 17
EagI C'GGCC_G
Cuts at: 4250 4250
Size: 6665
EarI CTCTTCn'nnn_
Cuts at: 1211 1541 1679 2050 3167 3200 4688 4898 1211
Size: 330 138 371 1117 33 1488 210 2978
Fragments arranged by size:
2978 1488 1117 371 330 210 138 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 297 2629 4873 5183 5329 6475 297
Size: 2332 2244 310 146 1146 487
Fragments arranged by size:
2332 2244 1146 487 310 146
EcoICRI GAG'CTC
Cuts at: 583 2961 3449 3677 583
Size: 2378 488 228 3571
Fragments arranged by size:
3571 2378 488 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 956 975 1290 1402 2146 2767 2801 3146 3191
Size: 19 315 112 744 621 34 345 45
Cuts at: 3191 3465 3478 4490 4922 5669 5937 956
Size: 274 13 1012 432 747 268 1684
Fragments arranged by size:
1684 1012 747 744 621 432 345 315
274 268 112 45 34 19 13
EcoNI CCTnn'n_nnAGG
Cuts at: 923 2720 923
Size: 1797 4868
EcoO109I rG'GnC_Cy
Cuts at: 601 667 928 1083 1090 2237 2351 2402 2532
Size: 66 261 155 7 1147 114 51 130
Cuts at: 2532 2547 2887 2888 3157 3177 3362 3539 3594
Size: 15 340 1 269 20 185 177 55
Cuts at: 3594 3804 601
Size: 210 3462
Fragments arranged by size:
3462 1147 340 269 261 210 185 177
155 130 114 66 55 51 20 15
7 1
EcoRI G'AATT_C
Cuts at: 3669 3669
Size: 6665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4684 4684
Size: 6665
FspI TGC'GCA
Cuts at: 4446 4446
Size: 6665
HaeII r_GCGC'y
Cuts at: 331 551 3263 3480 3514 4099 4347 5369 6348
Size: 220 2712 217 34 585 248 1022 979
Cuts at: 6348 331
Size: 648
Fragments arranged by size:
2712 1022 979 648 585 248 220 217
34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1972 2004 2359 2391 2866 2898 4507 4537 4539
Size: 32 355 32 475 32 1609 30 2
Cuts at: 4539 4569 1972
Size: 30 4068
Fragments arranged by size:
4068 1609 475 355 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 749 804 1030 2646 3704 749
Size: 55 226 1616 1058 3710
Fragments arranged by size:
3710 1616 1058 226 55
HpaI GTT'AAC
Cuts at: 749 749
Size: 6665
KasI G'GCGC_C
Cuts at: 4095 4343 4095
Size: 248 6417
KpnI G_GTAC'C
Cuts at: 788 3685 788
Size: 2897 3768
MluI A'CGCG_T
Cuts at: 6098 6098
Size: 6665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1667 5336 5520 1667
Size: 3669 184 2812
Fragments arranged by size:
3669 2812 184
MscI TGG'CCA
Cuts at: 1933 3067 3424 3559 4426 1933
Size: 1134 357 135 867 4172
Fragments arranged by size:
4172 1134 867 357 135
MslI CAynn'nnrTG
Cuts at: 340 700 1336 2200 2251 2495 2855 3490 3583
Size: 360 636 864 51 244 360 635 93
Cuts at: 3583 4654 4781 340
Size: 1071 127 2224
Fragments arranged by size:
2224 1071 864 636 635 360 360 244
127 93 51
MspA1I CmG'CkG
Cuts at: 318 396 1727 1909 2073 3000 3776 4090 4450
Size: 78 1331 182 164 927 776 314 360
Cuts at: 4450 5463 5708 318
Size: 1013 245 1275
Fragments arranged by size:
1331 1275 1013 927 776 360 314 245
182 164 78
NaeI GCC'GGC
Cuts at: 1695 4846 1695
Size: 3151 3514
NarI GG'CG_CC
Cuts at: 4096 4344 4096
Size: 248 6417
NgoMIV G'CCGG_C
Cuts at: 1693 4844 1693
Size: 3151 3514
NspI r_CATG'y
Cuts at: 688 969 1341 2250 3489 3718 4218 5125 688
Size: 281 372 909 1239 229 500 907 2228
Fragments arranged by size:
2228 1239 909 907 500 372 281 229
PciI A'CATG_T
Cuts at: 1337 5121 1337
Size: 3784 2881
PflMI CCAn_nnn'nTGG
Cuts at: 264 483 1296 1872 264
Size: 219 813 576 5057
Fragments arranged by size:
5057 813 576 219
PfoI T'CCnGG_A
Cuts at: 1604 1604
Size: 6665
PmeI GTTT'AAAC
Cuts at: 3664 3664
Size: 6665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 931 963 5804 5836 931
Size: 32 4841 32 1760
Fragments arranged by size:
4841 1760 32 32
PpuMI rG'GwC_Cy
Cuts at: 1083 1090 2532 3177 3362 3539 3594 1083
Size: 7 1442 645 185 177 55 4154
Fragments arranged by size:
4154 1442 645 185 177 55 7
PshAI GACnn'nnGTC
Cuts at: 1088 1088
Size: 6665
PspOMI G'GGCC_C
Cuts at: 666 1902 2887 666
Size: 1236 985 4444
Fragments arranged by size:
4444 1236 985
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1042 1074 1042
Size: 32 6633
PstI C_TGCA'G
Cuts at: 2546 3345 3712 4397 2546
Size: 799 367 685 4814
Fragments arranged by size:
4814 799 685 367
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6665
PvuII CAG'CTG
Cuts at: 318 396 2073 3000 4090 4450 318
Size: 78 1677 927 1090 360 2533
Fragments arranged by size:
2533 1677 1090 927 360 78
RsrII CG'GwC_CG
Cuts at: 4860 4860
Size: 6665
SacI G_AGCT'C
Cuts at: 585 2963 3451 3679 585
Size: 2378 488 228 3571
Fragments arranged by size:
3571 2378 488 228
SalI G'TCGA_C
Cuts at: 1028 3702 1028
Size: 2674 3991
SanDI GG'GwC_CC
Cuts at: 1090 2532 3594 1090
Size: 1442 1062 4161
Fragments arranged by size:
4161 1442 1062
SapI GCTCTTCn'nnn_
Cuts at: 1211 3200 4688 4898 1211
Size: 1989 1488 210 2978
Fragments arranged by size:
2978 1989 1488 210
SbfI CC_TGCA'GG
Cuts at: 2546 3712 2546
Size: 1166 5499
ScaI AGT'ACT
Cuts at: 3061 6182 3061
Size: 3121 3544
SexAI A'CCwGG_T
Cuts at: 882 882
Size: 6665
SfcI C'TryA_G
Cuts at: 33 2013 2542 3341 3708 4393 5386 5577 33
Size: 1980 529 799 367 685 993 191 1121
Fragments arranged by size:
1980 1121 993 799 685 529 367 191
SfoI GGC'GCC
Cuts at: 4097 4345 4097
Size: 248 6417
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6665
SgrAI Cr'CCGG_yG
Cuts at: 2610 2610
Size: 6665
SmaI CCC'GGG
Cuts at: 2686 3687 2686
Size: 1001 5664
SmlI C'TyrA_G
Cuts at: 2241 3820 4735 5227 5489 2241
Size: 1579 915 492 262 3417
Fragments arranged by size:
3417 1579 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6665
SphI G_CATG'C
Cuts at: 3489 3718 4218 3489
Size: 229 500 5936
Fragments arranged by size:
5936 500 229
StuI AGG'CCT
Cuts at: 2742 2742
Size: 6665
StyI C'CwwG_G
Cuts at: 650 924 1211 1410 3799 4153 650
Size: 274 287 199 2389 354 3162
Fragments arranged by size:
3162 2389 354 287 274 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3516 4248 4381 4840 5049 3516
Size: 732 133 459 209 5132
Fragments arranged by size:
5132 732 459 209 133
TatI w'GTAC_w
Cuts at: 2277 2573 3059 6180 2277
Size: 296 486 3121 2762
Fragments arranged by size:
3121 2762 486 296
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 615 647 848 880 6182 6214 615
Size: 32 201 32 5302 32 1066
Fragments arranged by size:
5302 1066 201 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2641 4462 2641
Size: 1821 4844
XbaI T'CTAG_A
Cuts at: 1035 3696 1035
Size: 2661 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 450 931 939 1356 2772 3237 3489 4160 450
Size: 481 8 417 1416 465 252 671 2955
Fragments arranged by size:
2955 1416 671 481 465 417 252 8
XmaI C'CCGG_G
Cuts at: 2684 3685 2684
Size: 1001 5664
XmnI GAAnn'nnTTC
Cuts at: 1205 1205
Size: 6665
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BbsI BbvCI
BcgI BfrBI BmtI BsiWI BssHII BstZ17I ClaI EcoRV
FseI FspAI HindIII MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PsiI PspXI SacII
SfiI SnaBI SrfI SspI SwaI XhoI ZraI