Restriction map for
pF1KB3030
(Circular) (Six-base) MAPSORT of: pF1KB3030.seq Check: 4222 from: 1 to: 5864
pF1KB3030 5864 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1343 1355 1386 2902 1343
Size: 12 31 1516 4305
Fragments arranged by size:
4305 1516 31 12
Acc65I G'GTAC_C
Cuts at: 2880 2880
Size: 5864
AclI AA'CG_TT
Cuts at: 5704 5704
Size: 5864
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2038 3689 4121 4474 5136 2038
Size: 1651 432 353 662 2766
Fragments arranged by size:
2766 1651 662 432 353
AflIII A'CryG_T
Cuts at: 1652 3216 5016 5297 1652
Size: 1564 1800 281 2219
Fragments arranged by size:
2219 1800 1564 281
AgeI A'CCGG_T
Cuts at: 1099 1099
Size: 5864
AleI CACnn'nnGTG
Cuts at: 307 776 1651 2581 2824 307
Size: 469 875 930 243 3347
Fragments arranged by size:
3347 930 875 469 243
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 143 2004 143
Size: 1861 4003
AlwNI CAG_nnn'CTG
Cuts at: 1560 3169 4607 1560
Size: 1609 1438 2817
Fragments arranged by size:
2817 1609 1438
ApaLI G'TGCA_C
Cuts at: 2574 4702 2574
Size: 2128 3736
ApoI r'AATT_y
Cuts at: 1716 2278 2868 5217 5473 1716
Size: 562 590 2349 256 2107
Fragments arranged by size:
2349 2107 590 562 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5864
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5864
AvaI C'yCGr_G
Cuts at: 969 1864 2313 2884 969
Size: 895 449 571 3949
Fragments arranged by size:
3949 895 571 449
BamHI G'GATC_C
Cuts at: 2889 2889
Size: 5864
BanI G'GyrC_C
Cuts at: 475 1410 1813 2880 3294 3542 3577 475
Size: 935 403 1067 414 248 35 2762
Fragments arranged by size:
2762 1067 935 414 403 248 35
BanII G_rGCy'C
Cuts at: 149 413 961 1829 2878 3908 149
Size: 264 548 868 1049 1030 2105
Fragments arranged by size:
2105 1049 1030 868 548 264
BbeI G_GCGC'C
Cuts at: 3298 3546 3298
Size: 248 5616
BbsI GAAGACnn'nnnn_
Cuts at: 1528 2117 1528
Size: 589 5275
BciVI GTATCCnnnnn_n'
Cuts at: 2633 3762 4818 5864 2633
Size: 1129 1056 1046 2633
Fragments arranged by size:
2633 1129 1056 1046
BclI T'GATC_A
Cuts at: 995 2339 3384 995
Size: 1344 1045 3475
Fragments arranged by size:
3475 1344 1045
BfrBI ATG'CAT
Cuts at: 2733 2733
Size: 5864
BglI GCCn_nnn'nGGC
Cuts at: 1276 2593 1276
Size: 1317 4547
BglII A'GATC_T
Cuts at: 221 437 1781 3379 5416 221
Size: 216 1344 1598 2037 669
Fragments arranged by size:
2037 1598 1344 669 216
BlpI GC'TnA_GC
Cuts at: 1078 1543 2976 1078
Size: 465 1433 3966
Fragments arranged by size:
3966 1433 465
Bme1580I G_kGCm'C
Cuts at: 802 1415 1515 1629 2578 3489 3582 4706 802
Size: 613 100 114 949 911 93 1124 1960
Fragments arranged by size:
1960 1124 949 911 613 114 100 93
BmgBI CAC'GTC
Cuts at: 2004 2004
Size: 5864
BmrI ACTGGGnnnn_n'
Cuts at: 565 3076 3157 3492 5636 565
Size: 2511 81 335 2144 793
Fragments arranged by size:
2511 2144 793 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 335 4231 335
Size: 3896 1968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 397 1582 305
Size: 92 1185 4587
Fragments arranged by size:
4587 1185 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1942 2419 2554 3040 3919 4633 4931 1942
Size: 477 135 486 879 714 298 2875
Fragments arranged by size:
2875 879 714 486 477 298 135
BsaI GGTCTCn'nnnn_
Cuts at: 202 618 793 1105 5568 202
Size: 416 175 312 4463 498
Fragments arranged by size:
4463 498 416 312 175
BsaAI yAC'GTr
Cuts at: 1653 3217 1653
Size: 1564 4300
BsaBI GATnn'nnATC
Cuts at: 6 874 3378 5415 6
Size: 868 2504 2037 455
Fragments arranged by size:
2504 2037 868 455
BsaHI Gr'CG_yC
Cuts at: 3295 3543 4245 5741 3295
Size: 248 702 1496 3418
Fragments arranged by size:
3418 1496 702 248
BsaWI w'CCGG_w
Cuts at: 674 1099 1154 1223 3053 3574 4252 4663 4810
Size: 425 55 69 1830 521 678 411 147
Cuts at: 4810 5084 674
Size: 274 1454
Fragments arranged by size:
1830 1454 678 521 425 411 274 147
69 55
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 705 1069 1330 1587 1945 2056 2185 705
Size: 364 261 257 358 111 129 4384
Fragments arranged by size:
4384 364 358 261 257 129 111
BseYI C'CCAG_C
Cuts at: 479 2600 3289 4047 4712 5120 479
Size: 2121 689 758 665 408 1223
Fragments arranged by size:
2121 1223 758 689 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1484 184
Size: 1300 4564
BsiEI CG_ry'CG
Cuts at: 104 1104 3406 3452 4682 104
Size: 1000 2302 46 1230 1286
Fragments arranged by size:
2302 1286 1230 1000 46
BsiHKAI G_wGCw'C
Cuts at: 149 1829 2542 2578 2878 3656 3846 4706 149
Size: 1680 713 36 300 778 190 860 1307
Fragments arranged by size:
1680 1307 860 778 713 300 190 36
BsmI GAATG_Cn'
Cuts at: 508 508
Size: 5864
BsmBI CGTCTCn'nnnn_
Cuts at: 650 650
Size: 5864
Bsp1286I G_dGCh'C
Cuts at: 149 413 802 961 1415 1515 1629 1829 2542
Size: 264 389 159 454 100 114 200 713
Cuts at: 2542 2578 2878 3489 3582 3656 3846 3908 4706
Size: 36 300 611 93 74 190 62 798
Cuts at: 4706 149
Size: 1307
Fragments arranged by size:
1307 798 713 611 454 389 300 264
200 190 159 114 100 93 74 62
36
BspEI T'CCGG_A
Cuts at: 674 1154 674
Size: 480 5384
BspHI T'CATG_A
Cuts at: 4296 4296
Size: 5864
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2914 3128 3430 3811 2914
Size: 214 302 381 4967
Fragments arranged by size:
4967 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1320 1439 2430 4156 4210 5694 5859 50
Size: 1270 119 991 1726 54 1484 165 55
Fragments arranged by size:
1726 1484 1270 991 165 119 55 54
BsrDI GCAATG_nn'
Cuts at: 494 2160 2187 3776 494
Size: 1666 27 1589 2582
Fragments arranged by size:
2582 1666 1589 27
BsrFI r'CCGG_y
Cuts at: 1099 1490 2033 3862 4043 1099
Size: 391 543 1829 181 2920
Fragments arranged by size:
2920 1829 543 391 181
BsrGI T'GTAC_A
Cuts at: 1007 1007
Size: 5864
BssSI C'ACGA_G
Cuts at: 4135 4843 4135
Size: 708 5156
BstAPI GCAn_nnn'nTGC
Cuts at: 815 2159 3241 815
Size: 1344 1082 3438
Fragments arranged by size:
3438 1344 1082
BstBI TT'CG_AA
Cuts at: 4225 4225
Size: 5864
BstEII G'GTnAC_C
Cuts at: 2007 2007
Size: 5864
BstXI CCAn_nnnn'nTGG
Cuts at: 645 2608 645
Size: 1963 3901
BstYI r'GATC_y
Cuts at: 1 221 357 437 1226 1781 2889 3356 3379
Size: 220 136 80 789 555 1108 467 23
Cuts at: 3379 3714 3960 4266 4278 4364 5416 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1108 1052 789 555 467 449 335 306
246 220 136 86 80 23 12
Bsu36I CC'TnA_GG
Cuts at: 452 452
Size: 5864
BtgI C'CryG_G
Cuts at: 1469 2652 1469
Size: 1183 4681
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 872 1436 1836 3814 3995 4158 872
Size: 564 400 1978 181 163 2578
Fragments arranged by size:
2578 1978 564 400 181 163
BtsI GCAGTG_nn'
Cuts at: 293 477 1458 1487 2819 3107 293
Size: 184 981 29 1332 288 3050
Fragments arranged by size:
3050 1332 981 288 184 29
ClaI AT'CG_AT
Cuts at: 2384 2384
Size: 5864
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 621 656 621
Size: 35 5829
DraI TTT'AAA
Cuts at: 2863 2863
Size: 5864
DrdI GACnn_nn'nnGTC
Cuts at: 1351 1840 3570 4914 1351
Size: 489 1730 1344 2301
Fragments arranged by size:
2301 1730 1344 489
EaeI y'GGCC_r
Cuts at: 1046 1214 1277 1496 2621 3449 3623 4014 4041
Size: 168 63 219 1125 828 174 391 27
Cuts at: 4041 5386 1046
Size: 1345 1524
Fragments arranged by size:
1524 1345 1125 828 391 219 174 168
63 27
EagI C'GGCC_G
Cuts at: 3449 3449
Size: 5864
EarI CTCTTCn'nnn_
Cuts at: 821 2198 2413 3887 4097 821
Size: 1377 215 1474 210 2588
Fragments arranged by size:
2588 1474 1377 215 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 882 1242 2226 4072 4814 4960 5674 882
Size: 360 984 1846 742 146 714 1072
Fragments arranged by size:
1846 1072 984 742 714 360 146
EcoICRI GAG'CTC
Cuts at: 147 1827 2876 147
Size: 1680 1049 3135
Fragments arranged by size:
3135 1680 1049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 397 1582 2038 3689 4121 4474 5136 305
Size: 92 1185 456 1651 432 353 662 1033
Fragments arranged by size:
1651 1185 1033 662 456 432 353 92
EcoNI CCTnn'n_nnAGG
Cuts at: 309 2017 309
Size: 1708 4156
EcoO109I rG'GnC_Cy
Cuts at: 893 1170 1725 2237 2693 2748 3003 893
Size: 277 555 512 456 55 255 3754
Fragments arranged by size:
3754 555 512 456 277 255 55
EcoRI G'AATT_C
Cuts at: 2868 2868
Size: 5864
EcoRV GAT'ATC
Cuts at: 708 708
Size: 5864
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3883 3883
Size: 5864
FspI TGC'GCA
Cuts at: 1266 2179 3645 1266
Size: 913 1466 3485
Fragments arranged by size:
3485 1466 913
HaeII r_GCGC'y
Cuts at: 2815 3298 3546 4776 5547 2815
Size: 483 248 1230 771 3132
Fragments arranged by size:
3132 1230 771 483 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 417 449 895 927 943 975 2405 2437 3706
Size: 32 446 32 16 32 1430 32 1269
Cuts at: 3706 3736 3738 3768 417
Size: 30 2 30 2513
Fragments arranged by size:
2513 1430 1269 446 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 174 1344 2903 174
Size: 1170 1559 3135
Fragments arranged by size:
3135 1559 1170
KasI G'GCGC_C
Cuts at: 3294 3542 3294
Size: 248 5616
KpnI G_GTAC'C
Cuts at: 2884 2884
Size: 5864
MluI A'CGCG_T
Cuts at: 5297 5297
Size: 5864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2064 4623 4807 2064
Size: 2559 184 3121
Fragments arranged by size:
3121 2559 184
MscI TGG'CCA
Cuts at: 1048 1216 3625 1048
Size: 168 2409 3287
Fragments arranged by size:
3287 2409 168
MslI CAynn'nnrTG
Cuts at: 307 776 844 1622 1651 1876 2581 2824 3853
Size: 469 68 778 29 225 705 243 1029
Cuts at: 3853 3980 307
Size: 127 2191
Fragments arranged by size:
2191 1029 778 705 469 243 225 127
68 29
MspA1I CmG'CkG
Cuts at: 483 1542 1557 2626 2798 2975 3289 3649 4433
Size: 1059 15 1069 172 177 314 360 784
Cuts at: 4433 4678 483
Size: 245 1669
Fragments arranged by size:
1669 1069 1059 784 360 314 245 177
172 15
NaeI GCC'GGC
Cuts at: 4045 4045
Size: 5864
NarI GG'CG_CC
Cuts at: 3295 3543 3295
Size: 248 5616
NcoI C'CATG_G
Cuts at: 1469 1469
Size: 5864
NgoMIV G'CCGG_C
Cuts at: 4043 4043
Size: 5864
NsiI A_TGCA'T
Cuts at: 2735 2735
Size: 5864
NspI r_CATG'y
Cuts at: 195 2917 3417 5020 195
Size: 2722 500 1603 1039
Fragments arranged by size:
2722 1603 1039 500
PciI A'CATG_T
Cuts at: 5016 5016
Size: 5864
PflMI CCAn_nnn'nTGG
Cuts at: 420 1560 420
Size: 1140 4724
PmeI GTTT'AAAC
Cuts at: 2863 2863
Size: 5864
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 4310
Size: 32 5832
PpuMI rG'GwC_Cy
Cuts at: 1170 1725 2693 2748 1170
Size: 555 968 55 4286
Fragments arranged by size:
4286 968 555 55
PspXI vC'TCGA_Gb
Cuts at: 1864 1864
Size: 5864
PstI C_TGCA'G
Cuts at: 285 1292 1541 2783 2911 3596 285
Size: 1007 249 1242 128 685 2553
Fragments arranged by size:
2553 1242 1007 685 249 128
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5864
PvuII CAG'CTG
Cuts at: 483 1542 1557 3289 3649 483
Size: 1059 15 1732 360 2698
Fragments arranged by size:
2698 1732 1059 360 15
RsrII CG'GwC_CG
Cuts at: 4059 4059
Size: 5864
SacI G_AGCT'C
Cuts at: 149 1829 2878 149
Size: 1680 1049 3135
Fragments arranged by size:
3135 1680 1049
SalI G'TCGA_C
Cuts at: 1342 2901 1342
Size: 1559 4305
SanDI GG'GwC_CC
Cuts at: 2693 2693
Size: 5864
SapI GCTCTTCn'nnn_
Cuts at: 2413 3887 4097 2413
Size: 1474 210 4180
Fragments arranged by size:
4180 1474 210
SbfI CC_TGCA'GG
Cuts at: 2911 2911
Size: 5864
ScaI AGT'ACT
Cuts at: 5381 5381
Size: 5864
SfcI C'TryA_G
Cuts at: 33 281 1288 1537 2779 2907 3592 4560 4751
Size: 248 1007 249 1242 128 685 968 191
Cuts at: 4751 33
Size: 1146
Fragments arranged by size:
1242 1146 1007 968 685 249 248 191
128
SfoI GGC'GCC
Cuts at: 3296 3544 3296
Size: 248 5616
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5864
SgrAI Cr'CCGG_yG
Cuts at: 2033 2033
Size: 5864
SmaI CCC'GGG
Cuts at: 971 2315 2886 971
Size: 1344 571 3949
Fragments arranged by size:
3949 1344 571
SmlI C'TyrA_G
Cuts at: 1864 1957 2434 2533 3019 3934 4648 4910 1864
Size: 93 477 99 486 915 714 262 2818
Fragments arranged by size:
2818 915 714 486 477 262 99 93
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5864
SphI G_CATG'C
Cuts at: 2917 3417 2917
Size: 500 5364
StyI C'CwwG_G
Cuts at: 623 1049 1469 1697 1704 2998 3352 623
Size: 426 420 228 7 1294 354 3135
Fragments arranged by size:
3135 1294 426 420 354 228 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 378 493 2347 2671 3447 3580 4039 4248 378
Size: 115 1854 324 776 133 459 209 1994
Fragments arranged by size:
1994 1854 776 459 324 209 133 115
TatI w'GTAC_w
Cuts at: 1007 1868 5379 1007
Size: 861 3511 1492
Fragments arranged by size:
3511 1492 861
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1149 1181 1743 1775 5381 5413 1149
Size: 32 562 32 3606 32 1600
Fragments arranged by size:
3606 1600 562 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2080 3661 2080
Size: 1581 4283
XbaI T'CTAG_A
Cuts at: 2895 2895
Size: 5864
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3359 3359
Size: 5864
XhoI C'TCGA_G
Cuts at: 1864 1864
Size: 5864
XmaI C'CCGG_G
Cuts at: 969 2313 2884 969
Size: 1344 571 3949
Fragments arranged by size:
3949 1344 571
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BmtI Bpu10I BsaXI BsiWI
BssHII BstZ17I DraIII FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PsrI SacII SexAI SfiI SnaBI
SrfI SspI StuI SwaI XmnI ZraI