Restriction map for
pF1KB3037
(Circular) (Six-base) MAPSORT of: pF1KB3037.seq Check: 7490 from: 1 to: 6563
pF1KB3037 6563 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 3601 3601
Size: 6563
Acc65I G'GTAC_C
Cuts at: 3579 3579
Size: 6563
AclI AA'CG_TT
Cuts at: 6403 6403
Size: 6563
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 746 2048 2273 2557 3029 3253 4388 4820
Size: 372 1302 225 284 472 224 1135 432
Cuts at: 4820 5567 5835 374
Size: 747 268 1102
Fragments arranged by size:
1302 1135 1102 747 472 432 372 284
268 225 224
AflIII A'CryG_T
Cuts at: 3915 5019 5996 3915
Size: 1104 977 4482
Fragments arranged by size:
4482 1104 977
AgeI A'CCGG_T
Cuts at: 1747 3368 4951 1747
Size: 1621 1583 3359
Fragments arranged by size:
3359 1621 1583
AleI CACnn'nnGTG
Cuts at: 254 254
Size: 6563
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2749 2749
Size: 6563
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 607 639 685 717 607
Size: 32 46 32 6453
Fragments arranged by size:
6453 46 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1135 3868 5435 1135
Size: 2733 1567 2263
Fragments arranged by size:
2733 2263 1567
ApaLI G'TGCA_C
Cuts at: 5333 5333
Size: 6563
ApoI r'AATT_y
Cuts at: 484 991 1230 1456 1579 1611 1976 2506 2626
Size: 507 239 226 123 32 365 530 120
Cuts at: 2626 2824 3478 3567 5916 6172 484
Size: 198 654 89 2349 256 875
Fragments arranged by size:
2349 875 654 530 507 365 256 239
226 198 123 120 89 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 6563
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6563
AvaI C'yCGr_G
Cuts at: 3583 3583
Size: 6563
BamHI G'GATC_C
Cuts at: 3588 3588
Size: 6563
BanI G'GyrC_C
Cuts at: 3579 3993 4241 4276 3579
Size: 414 248 35 5866
Fragments arranged by size:
5866 414 248 35
BanII G_rGCy'C
Cuts at: 1875 3152 3356 3577 4607 1875
Size: 1277 204 221 1030 3831
Fragments arranged by size:
3831 1277 1030 221 204
BbeI G_GCGC'C
Cuts at: 3997 4245 3997
Size: 248 6315
BbsI GAAGACnn'nnnn_
Cuts at: 737 1562 1910 2434 2509 3020 737
Size: 825 348 524 75 511 4280
Fragments arranged by size:
4280 825 524 511 348 75
BbvCI CC'TCA_GC
Cuts at: 3380 3380
Size: 6563
BciVI GTATCCnnnnn_n'
Cuts at: 681 2580 4461 5222 6563 681
Size: 1899 1881 761 1341 681
Fragments arranged by size:
1899 1881 1341 761 681
BclI T'GATC_A
Cuts at: 1037 2096 2844 4083 1037
Size: 1059 748 1239 3517
Fragments arranged by size:
3517 1239 1059 748
BfrBI ATG'CAT
Cuts at: 866 866
Size: 6563
BglII A'GATC_T
Cuts at: 306 1764 2683 3228 4078 6115 306
Size: 1458 919 545 850 2037 754
Fragments arranged by size:
2037 1458 919 850 754 545
BlpI GC'TnA_GC
Cuts at: 3675 3675
Size: 6563
Bme1580I G_kGCm'C
Cuts at: 4188 4281 5337 4188
Size: 93 1056 5414
Fragments arranged by size:
5414 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1072 3372 3775 3856 4191 6335 1072
Size: 2300 403 81 335 2144 1300
Fragments arranged by size:
2300 2144 1300 403 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4930 4930
Size: 6563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2072 2132 2606 3118 3301 2072
Size: 60 474 512 183 5334
Fragments arranged by size:
5334 512 474 183 60
Bpu10I CC'TnA_GC
Cuts at: 3350 3380 3350
Size: 30 6533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1789 1898 3739 4618 5110 5408 1789
Size: 109 1841 879 492 298 2944
Fragments arranged by size:
2944 1841 879 492 298 109
BsaI GGTCTCn'nnnn_
Cuts at: 1586 1672 2605 6267 1586
Size: 86 933 3662 1882
Fragments arranged by size:
3662 1882 933 86
BsaAI yAC'GTr
Cuts at: 3916 3916
Size: 6563
BsaBI GATnn'nnATC
Cuts at: 6 305 2843 4077 6114 6
Size: 299 2538 1234 2037 455
Fragments arranged by size:
2538 2037 1234 455 299
BsaHI Gr'CG_yC
Cuts at: 3994 4242 4944 6440 3994
Size: 248 702 1496 4117
Fragments arranged by size:
4117 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1747 3368 3752 4273 4951 5225 5372 5783 1747
Size: 1621 384 521 678 274 147 411 2527
Fragments arranged by size:
2527 1621 678 521 411 384 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3062 3092 3062
Size: 30 6533
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3127 3397 3127
Size: 270 6293
BseYI C'CCAG_C
Cuts at: 3988 4746 5323 5819 3988
Size: 758 577 496 4732
Fragments arranged by size:
4732 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 2954 3261 284
Size: 2670 307 3586
Fragments arranged by size:
3586 2670 307
BsiEI CG_ry'CG
Cuts at: 104 4105 4151 5359 104
Size: 4001 46 1208 1308
Fragments arranged by size:
4001 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3577 4355 4545 5337 3577
Size: 778 190 792 4803
Fragments arranged by size:
4803 792 778 190
BsmI GAATG_Cn'
Cuts at: 1933 2217 1933
Size: 284 6279
BsmBI CGTCTCn'nnnn_
Cuts at: 3065 3385 3065
Size: 320 6243
Bsp1286I G_dGCh'C
Cuts at: 1875 3152 3356 3577 4188 4281 4355 4545 4607
Size: 1277 204 221 611 93 74 190 62
Cuts at: 4607 5337 1875
Size: 730 3101
Fragments arranged by size:
3101 1277 730 611 221 204 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5783 5783
Size: 6563
BspHI T'CATG_A
Cuts at: 1422 2280 2490 5739 1422
Size: 858 210 3249 2246
Fragments arranged by size:
3249 2246 858 210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 217 3471 3613 3827 4129 4510 217
Size: 3254 142 214 302 381 2270
Fragments arranged by size:
3254 2270 381 302 214 142
BsrBI CCG'CTC
Cuts at: 50 4855 4909 6393 6558 50
Size: 4805 54 1484 165 55
Fragments arranged by size:
4805 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2456 4475 2456
Size: 2019 4544
BsrFI r'CCGG_y
Cuts at: 170 1747 3368 4561 4742 4951 170
Size: 1577 1621 1193 181 209 1782
Fragments arranged by size:
1782 1621 1577 1193 209 181
BsrGI T'GTAC_A
Cuts at: 1203 1203
Size: 6563
BssSI C'ACGA_G
Cuts at: 4834 5192 4834
Size: 358 6205
BstAPI GCAn_nnn'nTGC
Cuts at: 3940 3940
Size: 6563
BstBI TT'CG_AA
Cuts at: 4924 4924
Size: 6563
BstXI CCAn_nnnn'nTGG
Cuts at: 337 337
Size: 6563
BstYI r'GATC_y
Cuts at: 1 306 406 1764 2683 3228 3309 3588 4055
Size: 305 100 1358 919 545 81 279 467
Cuts at: 4055 4078 4413 4659 5671 5757 5769 6115 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1358 1012 919 545 467 449 346 335
305 279 246 100 86 81 23 12
Bsu36I CC'TnA_GG
Cuts at: 1550 1550
Size: 6563
BtgI C'CryG_G
Cuts at: 107 2236 2648 3137 107
Size: 2129 412 489 3533
Fragments arranged by size:
3533 2129 489 412
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4513 4694 4857 4513
Size: 181 163 6219
Fragments arranged by size:
6219 181 163
BtsI GCAGTG_nn'
Cuts at: 3124 3806 3124
Size: 682 5881
DraI TTT'AAA
Cuts at: 836 1727 3562 836
Size: 891 1835 3837
Fragments arranged by size:
3837 1835 891
DrdI GACnn_nn'nnGTC
Cuts at: 2448 4269 5127 2448
Size: 1821 858 3884
Fragments arranged by size:
3884 1821 858
EaeI y'GGCC_r
Cuts at: 594 4148 4322 4713 4740 6085 594
Size: 3554 174 391 27 1345 1072
Fragments arranged by size:
3554 1345 1072 391 174 27
EagI C'GGCC_G
Cuts at: 4148 4148
Size: 6563
EarI CTCTTCn'nnn_
Cuts at: 1358 1580 1622 1794 1856 2753 3229 4586 4796
Size: 222 42 172 62 897 476 1357 210
Cuts at: 4796 1358
Size: 3125
Fragments arranged by size:
3125 1357 897 476 222 210 172 62
42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4771 5081 5227 6373 4771
Size: 310 146 1146 4961
Fragments arranged by size:
4961 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3575 3575
Size: 6563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 746 2048 2072 2132 2273 2557 2606 3029
Size: 372 1302 24 60 141 284 49 423
Cuts at: 3029 3118 3253 3301 4388 4820 5567 5835 374
Size: 89 135 48 1087 432 747 268 1102
Fragments arranged by size:
1302 1102 1087 747 432 423 372 284
268 141 135 89 60 49 48 24
EcoO109I rG'GnC_Cy
Cuts at: 3702 3702
Size: 6563
EcoRI G'AATT_C
Cuts at: 991 2626 3567 991
Size: 1635 941 3987
Fragments arranged by size:
3987 1635 941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 819 4582 819
Size: 3763 2800
FspI TGC'GCA
Cuts at: 4344 4344
Size: 6563
HaeII r_GCGC'y
Cuts at: 3997 4245 5267 6246 3997
Size: 248 1022 979 4314
Fragments arranged by size:
4314 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 440 472 1549 1581 1642 1674 1762
Size: 32 110 32 1077 32 61 32 88
Cuts at: 1762 1794 2485 2517 2776 2808 4405 4435 4437
Size: 32 691 32 259 32 1597 30 2
Cuts at: 4437 4467 298
Size: 30 2394
Fragments arranged by size:
2394 1597 1077 691 259 110 88 61
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1745 2679 3602 1745
Size: 934 923 4706
Fragments arranged by size:
4706 934 923
HindIII A'AGCT_T
Cuts at: 3535 3535
Size: 6563
KasI G'GCGC_C
Cuts at: 3993 4241 3993
Size: 248 6315
KpnI G_GTAC'C
Cuts at: 3583 3583
Size: 6563
MluI A'CGCG_T
Cuts at: 5996 5996
Size: 6563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5234 5418 5234
Size: 184 6379
MscI TGG'CCA
Cuts at: 596 4324 596
Size: 3728 2835
MslI CAynn'nnrTG
Cuts at: 254 283 335 796 1498 2461 2492 2495 2807
Size: 29 52 461 702 963 31 3 312
Cuts at: 2807 3061 4552 4679 254
Size: 254 1491 127 2138
Fragments arranged by size:
2138 1491 963 702 461 312 254 127
52 31 29 3
MspA1I CmG'CkG
Cuts at: 3053 3202 3379 3674 3988 4348 5361 5606 3053
Size: 149 177 295 314 360 1013 245 4010
Fragments arranged by size:
4010 1013 360 314 295 245 177 149
NaeI GCC'GGC
Cuts at: 4744 4744
Size: 6563
NarI GG'CG_CC
Cuts at: 3994 4242 3994
Size: 248 6315
NcoI C'CATG_G
Cuts at: 107 3137 107
Size: 3030 3533
NgoMIV G'CCGG_C
Cuts at: 4742 4742
Size: 6563
NsiI A_TGCA'T
Cuts at: 868 868
Size: 6563
NspI r_CATG'y
Cuts at: 1471 3616 4116 5023 1471
Size: 2145 500 907 3011
Fragments arranged by size:
3011 2145 907 500
PasI CC'CwG_GG
Cuts at: 392 392
Size: 6563
PciI A'CATG_T
Cuts at: 5019 5019
Size: 6563
PflMI CCAn_nnn'nTGG
Cuts at: 628 1084 628
Size: 456 6107
PfoI T'CCnGG_A
Cuts at: 1889 3428 1889
Size: 1539 5024
PmeI GTTT'AAAC
Cuts at: 3562 3562
Size: 6563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3063 5702 5734 3031
Size: 32 2639 32 3860
Fragments arranged by size:
3860 2639 32 32
PsiI TTA'TAA
Cuts at: 602 602
Size: 6563
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 173 205 173
Size: 32 6531
PstI C_TGCA'G
Cuts at: 2418 2539 3610 4295 2418
Size: 121 1071 685 4686
Fragments arranged by size:
4686 1071 685 121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6563
PvuII CAG'CTG
Cuts at: 3202 3988 4348 3202
Size: 786 360 5417
Fragments arranged by size:
5417 786 360
RsrII CG'GwC_CG
Cuts at: 4758 4758
Size: 6563
SacI G_AGCT'C
Cuts at: 3577 3577
Size: 6563
SalI G'TCGA_C
Cuts at: 3600 3600
Size: 6563
SapI GCTCTTCn'nnn_
Cuts at: 4586 4796 4586
Size: 210 6353
SbfI CC_TGCA'GG
Cuts at: 3610 3610
Size: 6563
ScaI AGT'ACT
Cuts at: 138 3103 6080 138
Size: 2965 2977 621
Fragments arranged by size:
2977 2965 621
SexAI A'CCwGG_T
Cuts at: 955 955
Size: 6563
SfcI C'TryA_G
Cuts at: 33 150 1981 2025 2271 2414 2535 3606 4291
Size: 117 1831 44 246 143 121 1071 685
Cuts at: 4291 5284 5475 33
Size: 993 191 1121
Fragments arranged by size:
1831 1121 1071 993 685 246 191 143
121 117 44
SfoI GGC'GCC
Cuts at: 3995 4243 3995
Size: 248 6315
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6563
SmaI CCC'GGG
Cuts at: 3585 3585
Size: 6563
SmlI C'TyrA_G
Cuts at: 1768 1913 3718 4633 5125 5387 1768
Size: 145 1805 915 492 262 2944
Fragments arranged by size:
2944 1805 915 492 262 145
SpeI A'CTAG_T
Cuts at: 67 1920 67
Size: 1853 4710
SphI G_CATG'C
Cuts at: 3616 4116 3616
Size: 500 6063
SspI AAT'ATT
Cuts at: 2666 3503 2666
Size: 837 5726
StuI AGG'CCT
Cuts at: 376 376
Size: 6563
StyI C'CwwG_G
Cuts at: 107 3137 3697 4051 107
Size: 3030 560 354 2619
Fragments arranged by size:
3030 2619 560 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1809 4146 4279 4738 4947 1809
Size: 2337 133 459 209 3425
Fragments arranged by size:
3425 2337 459 209 133
TatI w'GTAC_w
Cuts at: 136 1203 3078 3101 6078 136
Size: 1067 1875 23 2977 621
Fragments arranged by size:
2977 1875 1067 621 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6080 6112 6080
Size: 32 6531
Tth111I GACn'n_nGTC
Cuts at: 1418 4360 1418
Size: 2942 3621
XbaI T'CTAG_A
Cuts at: 1020 2544 3594 1020
Size: 1524 1050 3989
Fragments arranged by size:
3989 1524 1050
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3093 4058 3093
Size: 965 5598
XmaI C'CCGG_G
Cuts at: 3583 3583
Size: 6563
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BcgI BglI BmgBI BmtI BsiWI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PpuMI PshAI PspOMI PspXI SacII SanDI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI