Restriction map for
pF1KB3038
(Circular) (Six-base) MAPSORT of: pF1KB3038.seq Check: 5405 from: 1 to: 7316
pF1KB3038 7316 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 524 1332 4354 524
Size: 808 3022 3486
Fragments arranged by size:
3486 3022 808
Acc65I G'GTAC_C
Cuts at: 4332 4332
Size: 7316
AclI AA'CG_TT
Cuts at: 7156 7156
Size: 7316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 2252 2552 2818 3638 4027 5141 5573 6320
Size: 1863 300 266 820 389 1114 432 747
Cuts at: 6320 6588 389
Size: 268 1117
Fragments arranged by size:
1863 1117 1114 820 747 432 389 300
268 266
AflII C'TTAA_G
Cuts at: 3037 3037
Size: 7316
AflIII A'CryG_T
Cuts at: 4668 5772 6749 4668
Size: 1104 977 5235
Fragments arranged by size:
5235 1104 977
AgeI A'CCGG_T
Cuts at: 5704 5704
Size: 7316
AhdI GACnn_n'nnGTC
Cuts at: 657 657
Size: 7316
AlwNI CAG_nnn'CTG
Cuts at: 1584 1723 2380 3778 4621 6188 1584
Size: 139 657 1398 843 1567 2712
Fragments arranged by size:
2712 1567 1398 843 657 139
ApaLI G'TGCA_C
Cuts at: 4190 6086 4190
Size: 1896 5420
ApoI r'AATT_y
Cuts at: 611 725 1673 3666 4320 6669 6925 611
Size: 114 948 1993 654 2349 256 1002
Fragments arranged by size:
2349 1993 1002 948 654 256 114
AseI AT'TA_AT
Cuts at: 20 2669 20
Size: 2649 4667
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7316
AvaI C'yCGr_G
Cuts at: 4336 4336
Size: 7316
AvrII C'CTAG_G
Cuts at: 1179 1179
Size: 7316
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 453 486 453
Size: 33 7283
BamHI G'GATC_C
Cuts at: 3144 4341 3144
Size: 1197 6119
BanI G'GyrC_C
Cuts at: 304 1414 2286 4332 4746 4994 5029 304
Size: 1110 872 2046 414 248 35 2591
Fragments arranged by size:
2591 2046 1110 872 414 248 35
BanII G_rGCy'C
Cuts at: 2701 3036 4330 5360 2701
Size: 335 1294 1030 4657
Fragments arranged by size:
4657 1294 1030 335
BbeI G_GCGC'C
Cuts at: 308 4750 4998 308
Size: 4442 248 2626
Fragments arranged by size:
4442 2626 248
BbsI GAAGACnn'nnnn_
Cuts at: 3985 3985
Size: 7316
BbvCI CC'TCA_GC
Cuts at: 2441 2441
Size: 7316
BciVI GTATCCnnnnn_n'
Cuts at: 5214 5975 7316 5214
Size: 761 1341 5214
Fragments arranged by size:
5214 1341 761
BclI T'GATC_A
Cuts at: 4836 4836
Size: 7316
BfrBI ATG'CAT
Cuts at: 541 864 541
Size: 323 6993
BglII A'GATC_T
Cuts at: 876 2712 3187 3702 4831 6868 876
Size: 1836 475 515 1129 2037 1324
Fragments arranged by size:
2037 1836 1324 1129 515 475
BlpI GC'TnA_GC
Cuts at: 2784 4428 2784
Size: 1644 5672
Bme1580I G_kGCm'C
Cuts at: 4194 4941 5034 6090 4194
Size: 747 93 1056 5420
Fragments arranged by size:
5420 1056 747 93
BmrI ACTGGGnnnn_n'
Cuts at: 334 2105 3150 3636 4528 4609 4944 7088 334
Size: 1771 1045 486 892 81 335 2144 562
Fragments arranged by size:
2144 1771 1045 892 562 486 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 145 1419 4049 5683 145
Size: 1274 2630 1634 1778
Fragments arranged by size:
2630 1778 1634 1274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1389 1466 1796 2098 2330 3050 3770 1389
Size: 77 330 302 232 720 720 4935
Fragments arranged by size:
4935 720 720 330 302 232 77
Bpu10I CC'TnA_GC
Cuts at: 2441 2441
Size: 7316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4492 5371 5863 6161 4492
Size: 879 492 298 5647
Fragments arranged by size:
5647 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1632 3960 7020 1632
Size: 2328 3060 1928
Fragments arranged by size:
3060 2328 1928
BsaAI yAC'GTr
Cuts at: 3019 4669 3019
Size: 1650 5666
BsaBI GATnn'nnATC
Cuts at: 6 4830 6867 6
Size: 4824 2037 455
Fragments arranged by size:
4824 2037 455
BsaHI Gr'CG_yC
Cuts at: 217 305 4747 4995 5697 7193 217
Size: 88 4442 248 702 1496 340
Fragments arranged by size:
4442 1496 702 340 248 88
BsaWI w'CCGG_w
Cuts at: 4505 5026 5704 5978 6125 6536 4505
Size: 521 678 274 147 411 5285
Fragments arranged by size:
5285 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2577 2607 3211 3241 3743 3773 4269 4299 2577
Size: 30 604 30 502 30 496 30 5594
Fragments arranged by size:
5594 604 502 496 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 253 1381 2008 2196 3219 3339 3342 4277
Size: 27 1128 627 188 1023 120 3 935
Cuts at: 4277 226
Size: 3265
Fragments arranged by size:
3265 1128 1023 935 627 188 120 27
3
BseYI C'CCAG_C
Cuts at: 223 589 1860 2105 2520 2779 4741 5499 6076
Size: 366 1271 245 415 259 1962 758 577
Cuts at: 6076 6572 223
Size: 496 967
Fragments arranged by size:
1962 1271 967 758 577 496 415 366
259 245
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 690 806 1079 1256 690
Size: 116 273 177 6750
Fragments arranged by size:
6750 273 177 116
BsiEI CG_ry'CG
Cuts at: 104 4858 4904 6112 104
Size: 4754 46 1208 1308
Fragments arranged by size:
4754 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3036 4194 4330 5108 5298 6090 3036
Size: 1158 136 778 190 792 4262
Fragments arranged by size:
4262 1158 792 778 190 136
BsmI GAATG_Cn'
Cuts at: 498 498
Size: 7316
BsmBI CGTCTCn'nnnn_
Cuts at: 1770 1770
Size: 7316
Bsp1286I G_dGCh'C
Cuts at: 2701 3036 4194 4330 4941 5034 5108 5298 5360
Size: 335 1158 136 611 93 74 190 62
Cuts at: 5360 6090 2701
Size: 730 3927
Fragments arranged by size:
3927 1158 730 611 335 190 136 93
74 62
BspEI T'CCGG_A
Cuts at: 6536 6536
Size: 7316
BspHI T'CATG_A
Cuts at: 294 6492 294
Size: 6198 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1943 2053 4366 4580 4882 5263 1943
Size: 110 2313 214 302 381 3996
Fragments arranged by size:
3996 2313 381 302 214 110
BsrBI CCG'CTC
Cuts at: 50 200 5608 5662 7146 7311 50
Size: 150 5408 54 1484 165 55
Fragments arranged by size:
5408 1484 165 150 55 54
BsrDI GCAATG_nn'
Cuts at: 737 3815 4018 4037 4176 5228 737
Size: 3078 203 19 139 1052 2825
Fragments arranged by size:
3078 2825 1052 203 139 19
BsrFI r'CCGG_y
Cuts at: 5314 5495 5704 5314
Size: 181 209 6926
Fragments arranged by size:
6926 209 181
BsrGI T'GTAC_A
Cuts at: 1140 2986 1140
Size: 1846 5470
BssSI C'ACGA_G
Cuts at: 5587 5945 5587
Size: 358 6958
BstAPI GCAn_nnn'nTGC
Cuts at: 448 4693 448
Size: 4245 3071
BstBI TT'CG_AA
Cuts at: 5677 5677
Size: 7316
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2122
Size: 7316
BstYI r'GATC_y
Cuts at: 1 876 945 2712 3144 3187 3702 3960 4341
Size: 875 69 1767 432 43 515 258 381
Cuts at: 4341 4808 4831 5166 5412 6424 6510 6522 6868
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6868 1
Size: 449
Fragments arranged by size:
1767 1012 875 515 467 449 432 381
346 335 258 246 86 69 43 23
12
Bsu36I CC'TnA_GG
Cuts at: 1211 2223 1211
Size: 1012 6304
BtgI C'CryG_G
Cuts at: 1109 1152 1999 2397 3914 1109
Size: 43 847 398 1517 4511
Fragments arranged by size:
4511 1517 847 398 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5266 5447 5610 5266
Size: 181 163 6972
Fragments arranged by size:
6972 181 163
BtsI GCAGTG_nn'
Cuts at: 1702 2213 2327 3869 4559 1702
Size: 511 114 1542 690 4459
Fragments arranged by size:
4459 1542 690 511 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1437 1472 1437
Size: 35 7281
DraI TTT'AAA
Cuts at: 668 1568 3345 4315 668
Size: 900 1777 970 3669
Fragments arranged by size:
3669 1777 970 900
DrdI GACnn_nn'nnGTC
Cuts at: 5022 5880 5022
Size: 858 6458
EaeI y'GGCC_r
Cuts at: 957 1155 1725 1808 4901 5075 5466 5493 6838
Size: 198 570 83 3093 174 391 27 1345
Cuts at: 6838 957
Size: 1435
Fragments arranged by size:
3093 1435 1345 570 391 198 174 83
27
EagI C'GGCC_G
Cuts at: 4901 4901
Size: 7316
EarI CTCTTCn'nnn_
Cuts at: 2094 2562 5339 5549 2094
Size: 468 2777 210 3861
Fragments arranged by size:
3861 2777 468 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 272 4086 4290 5524 5834 5980 7126 272
Size: 3814 204 1234 310 146 1146 462
Fragments arranged by size:
3814 1234 1146 462 310 204 146
EcoICRI GAG'CTC
Cuts at: 3034 4328 3034
Size: 1294 6022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1389 1466 1796 2098 2252 2330 2552 2818
Size: 1000 77 330 302 154 78 222 266
Cuts at: 2818 3050 3638 3770 4027 5141 5573 6320 6588
Size: 232 588 132 257 1114 432 747 268
Cuts at: 6588 389
Size: 1117
Fragments arranged by size:
1117 1114 1000 747 588 432 330 302
268 266 257 232 222 154 132 78
77
EcoO109I rG'GnC_Cy
Cuts at: 318 2065 3337 4251 4455 318
Size: 1747 1272 914 204 3179
Fragments arranged by size:
3179 1747 1272 914 204
EcoRI G'AATT_C
Cuts at: 1673 4320 1673
Size: 2647 4669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5335 5335
Size: 7316
FspI TGC'GCA
Cuts at: 5097 5097
Size: 7316
HaeII r_GCGC'y
Cuts at: 253 308 4750 4998 6020 6999 253
Size: 55 4442 248 1022 979 570
Fragments arranged by size:
4442 1022 979 570 248 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 643 675 753 785 798 830 2395
Size: 32 409 32 78 32 13 32 1565
Cuts at: 2395 2427 3283 3315 3743 3775 5158 5188 5190
Size: 32 856 32 428 32 1383 30 2
Cuts at: 5190 5220 202
Size: 30 2298
Fragments arranged by size:
2298 1565 1383 856 428 409 78 32
32 32 32 32 32 32 30 30
13 2
HincII GTy'rAC
Cuts at: 693 2727 3573 4355 693
Size: 2034 846 782 3654
Fragments arranged by size:
3654 2034 846 782
HindIII A'AGCT_T
Cuts at: 785 3967 785
Size: 3182 4134
HpaI GTT'AAC
Cuts at: 2727 3573 2727
Size: 846 6470
KasI G'GCGC_C
Cuts at: 304 4746 4994 304
Size: 4442 248 2626
Fragments arranged by size:
4442 2626 248
KpnI G_GTAC'C
Cuts at: 4336 4336
Size: 7316
MfeI C'AATT_G
Cuts at: 1144 1449 3200 1144
Size: 305 1751 5260
Fragments arranged by size:
5260 1751 305
MluI A'CGCG_T
Cuts at: 6749 6749
Size: 7316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 838 2831 5987 6171 838
Size: 1993 3156 184 1983
Fragments arranged by size:
3156 1993 1983 184
MscI TGG'CCA
Cuts at: 959 1157 1727 1810 5077 959
Size: 198 570 83 3267 3198
Fragments arranged by size:
3267 3198 570 198 83
MslI CAynn'nnrTG
Cuts at: 299 536 546 749 1157 5305 5432 299
Size: 237 10 203 408 4148 127 2183
Fragments arranged by size:
4148 2183 408 237 203 127 10
MspA1I CmG'CkG
Cuts at: 145 1599 1693 2122 2524 4427 4741 5101 6114
Size: 1454 94 429 402 1903 314 360 1013
Cuts at: 6114 6359 145
Size: 245 1102
Fragments arranged by size:
1903 1454 1102 1013 429 402 360 314
245 94
NaeI GCC'GGC
Cuts at: 5497 5497
Size: 7316
NarI GG'CG_CC
Cuts at: 305 4747 4995 305
Size: 4442 248 2626
Fragments arranged by size:
4442 2626 248
NcoI C'CATG_G
Cuts at: 1109 1152 2397 3914 1109
Size: 43 1245 1517 4511
Fragments arranged by size:
4511 1517 1245 43
NdeI CA'TA_TG
Cuts at: 3095 3095
Size: 7316
NgoMIV G'CCGG_C
Cuts at: 5495 5495
Size: 7316
NsiI A_TGCA'T
Cuts at: 543 866 543
Size: 323 6993
NspI r_CATG'y
Cuts at: 1468 2807 4369 4869 5776 1468
Size: 1339 1562 500 907 3008
Fragments arranged by size:
3008 1562 1339 907 500
PasI CC'CwG_GG
Cuts at: 1795 1795
Size: 7316
PciI A'CATG_T
Cuts at: 5772 5772
Size: 7316
PfoI T'CCnGG_A
Cuts at: 3236 3928 3236
Size: 692 6624
PmeI GTTT'AAAC
Cuts at: 4315 4315
Size: 7316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2069 2101 6455 6487 2069
Size: 32 4354 32 2898
Fragments arranged by size:
4354 2898 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 3337 4251 2065
Size: 1272 914 5130
Fragments arranged by size:
5130 1272 914
PsiI TTA'TAA
Cuts at: 536 536
Size: 7316
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 7284
PstI C_TGCA'G
Cuts at: 1167 1830 1940 4047 4363 5048 1167
Size: 663 110 2107 316 685 3435
Fragments arranged by size:
3435 2107 685 663 316 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7316
PvuII CAG'CTG
Cuts at: 1599 1693 2122 2524 4741 5101 1599
Size: 94 429 402 2217 360 3814
Fragments arranged by size:
3814 2217 429 402 360 94
RsrII CG'GwC_CG
Cuts at: 5511 5511
Size: 7316
SacI G_AGCT'C
Cuts at: 3036 4330 3036
Size: 1294 6022
SalI G'TCGA_C
Cuts at: 4353 4353
Size: 7316
SapI GCTCTTCn'nnn_
Cuts at: 5339 5549 5339
Size: 210 7106
SbfI CC_TGCA'GG
Cuts at: 4363 4363
Size: 7316
ScaI AGT'ACT
Cuts at: 6833 6833
Size: 7316
SexAI A'CCwGG_T
Cuts at: 2415 3537 2415
Size: 1122 6194
SfcI C'TryA_G
Cuts at: 33 465 872 1163 1333 1826 1842 1936 4043
Size: 432 407 291 170 493 16 94 2107
Cuts at: 4043 4098 4359 5044 6037 6228 33
Size: 55 261 685 993 191 1121
Fragments arranged by size:
2107 1121 993 685 493 432 407 291
261 191 170 94 55 16
SfoI GGC'GCC
Cuts at: 306 4748 4996 306
Size: 4442 248 2626
Fragments arranged by size:
4442 2626 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7316
SmaI CCC'GGG
Cuts at: 4338 4338
Size: 7316
SmlI C'TyrA_G
Cuts at: 3037 4471 5386 5878 6140 3037
Size: 1434 915 492 262 4213
Fragments arranged by size:
4213 1434 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7316
SphI G_CATG'C
Cuts at: 2807 4369 4869 2807
Size: 1562 500 5254
Fragments arranged by size:
5254 1562 500
SspI AAT'ATT
Cuts at: 3369 3582 3369
Size: 213 7103
StuI AGG'CCT
Cuts at: 1126 3226 1126
Size: 2100 5216
StyI C'CwwG_G
Cuts at: 605 899 935 1109 1152 1179 2397 3914 4450
Size: 294 36 174 43 27 1218 1517 536
Cuts at: 4450 4804 605
Size: 354 3117
Fragments arranged by size:
3117 1517 1218 536 354 294 174 43
36 27
SwaI ATTT'AAAT
Cuts at: 1568 1568
Size: 7316
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2534 4899 5032 5491 5700 2534
Size: 2365 133 459 209 4150
Fragments arranged by size:
4150 2365 459 209 133
TatI w'GTAC_w
Cuts at: 1140 1485 1867 2986 4173 4238 6831 1140
Size: 345 382 1119 1187 65 2593 1625
Fragments arranged by size:
2593 1625 1187 1119 382 345 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1454 1486 6833 6865 1454
Size: 32 5347 32 1905
Fragments arranged by size:
5347 1905 32 32
Tth111I GACn'n_nGTC
Cuts at: 2503 5113 2503
Size: 2610 4706
XbaI T'CTAG_A
Cuts at: 4347 4347
Size: 7316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2478 4811 2478
Size: 2333 4983
XmaI C'CCGG_G
Cuts at: 4336 4336
Size: 7316
XmnI GAAnn'nnTTC
Cuts at: 3643 3643
Size: 7316
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PfoI PmeI PpiI PpuMI PsiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI ApaI AscI
BcgI BglI BmgBI BmtI BsiWI BssHII BstEII BstZ17I
ClaI DraIII EcoNI EcoRV FseI FspAI NheI NotI
NruI PacI PflMI PmlI PshAI PspOMI PspXI SacII
SanDI SfiI SgrAI SnaBI SrfI XhoI ZraI