Restriction map for
pF1KB3039
(Circular) (Six-base) MAPSORT of: pF1KB3039.seq Check: 6220 from: 1 to: 6485
pF1KB3039 6485 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 244 647 244
Size: 403 6082
AccI GT'mk_AC
Cuts at: 3523 3523
Size: 6485
Acc65I G'GTAC_C
Cuts at: 3501 3501
Size: 6485
AclI AA'CG_TT
Cuts at: 6325 6325
Size: 6485
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3488 4310 4742 5489 5757 3488
Size: 822 432 747 268 4216
Fragments arranged by size:
4216 822 747 432 268
AflII C'TTAA_G
Cuts at: 2623 2623
Size: 6485
AflIII A'CryG_T
Cuts at: 856 881 3837 4941 5918 856
Size: 25 2956 1104 977 1423
Fragments arranged by size:
2956 1423 1104 977 25
AgeI A'CCGG_T
Cuts at: 4873 4873
Size: 6485
AlwNI CAG_nnn'CTG
Cuts at: 1396 3790 5357 1396
Size: 2394 1567 2524
Fragments arranged by size:
2524 2394 1567
ApaLI G'TGCA_C
Cuts at: 5255 5255
Size: 6485
ApoI r'AATT_y
Cuts at: 1201 1785 2581 3489 5838 6094 1201
Size: 584 796 908 2349 256 1592
Fragments arranged by size:
2349 1592 908 796 584 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6485
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6485
AvaI C'yCGr_G
Cuts at: 2954 3065 3505 2954
Size: 111 440 5934
Fragments arranged by size:
5934 440 111
BamHI G'GATC_C
Cuts at: 315 1346 3510 315
Size: 1031 2164 3290
Fragments arranged by size:
3290 2164 1031
BanI G'GyrC_C
Cuts at: 172 254 3501 3915 4163 4198 172
Size: 82 3247 414 248 35 2459
Fragments arranged by size:
3247 2459 414 248 82 35
BanII G_rGCy'C
Cuts at: 269 1121 3499 4529 269
Size: 852 2378 1030 2225
Fragments arranged by size:
2378 2225 1030 852
BbeI G_GCGC'C
Cuts at: 258 3919 4167 258
Size: 3661 248 2576
Fragments arranged by size:
3661 2576 248
BbsI GAAGACnn'nnnn_
Cuts at: 2156 2156
Size: 6485
BbvCI CC'TCA_GC
Cuts at: 2067 2067
Size: 6485
BciVI GTATCCnnnnn_n'
Cuts at: 437 4383 5144 6485 437
Size: 3946 761 1341 437
Fragments arranged by size:
3946 1341 761 437
BclI T'GATC_A
Cuts at: 191 1866 2408 2700 4005 191
Size: 1675 542 292 1305 2671
Fragments arranged by size:
2671 1675 1305 542 292
BfrBI ATG'CAT
Cuts at: 827 827
Size: 6485
BglI GCCn_nnn'nGGC
Cuts at: 2950 2950
Size: 6485
BglII A'GATC_T
Cuts at: 1989 4000 6037 1989
Size: 2011 2037 2437
Fragments arranged by size:
2437 2037 2011
BlpI GC'TnA_GC
Cuts at: 650 3597 650
Size: 2947 3538
Bme1580I G_kGCm'C
Cuts at: 605 4110 4203 5259 605
Size: 3505 93 1056 1831
Fragments arranged by size:
3505 1831 1056 93
BmgBI CAC'GTC
Cuts at: 857 2949 857
Size: 2092 4393
BmrI ACTGGGnnnn_n'
Cuts at: 3103 3697 3778 4113 6257 3103
Size: 594 81 335 2144 3331
Fragments arranged by size:
3331 2144 594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 715 4852 715
Size: 4137 2348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 839 1088 1105 2887 3298 686
Size: 153 249 17 1782 411 3873
Fragments arranged by size:
3873 1782 411 249 153 17
Bpu10I CC'TnA_GC
Cuts at: 1045 2067 3207 1045
Size: 1022 1140 4323
Fragments arranged by size:
4323 1140 1022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 2188 2297 2991 3661 4540 5032 5330 317
Size: 1871 109 694 670 879 492 298 1472
Fragments arranged by size:
1871 1472 879 694 670 492 298 109
BsaI GGTCTCn'nnnn_
Cuts at: 6189 6189
Size: 6485
BsaAI yAC'GTr
Cuts at: 1941 3414 3838 1941
Size: 1473 424 4588
Fragments arranged by size:
4588 1473 424
BsaBI GATnn'nnATC
Cuts at: 6 190 3124 3999 6036 6
Size: 184 2934 875 2037 455
Fragments arranged by size:
2934 2037 875 455 184
BsaHI Gr'CG_yC
Cuts at: 255 3916 4164 4866 6362 255
Size: 3661 248 702 1496 378
Fragments arranged by size:
3661 1496 702 378 248
BsaWI w'CCGG_w
Cuts at: 731 1343 3674 4195 4873 5147 5294 5705 731
Size: 612 2331 521 678 274 147 411 1511
Fragments arranged by size:
2331 1511 678 612 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 506 536 1358 1388 1834 1864 506
Size: 30 822 30 446 30 5127
Fragments arranged by size:
5127 822 446 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 443 1730 2015 2055 3412 443
Size: 1287 285 40 1357 3516
Fragments arranged by size:
3516 1357 1287 285 40
BseYI C'CCAG_C
Cuts at: 1396 2274 2877 2921 3910 4668 5245 5741 1396
Size: 878 603 44 989 758 577 496 2140
Fragments arranged by size:
2140 989 878 758 603 577 496 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 930 1071 2509 3260 862
Size: 68 141 1438 751 4087
Fragments arranged by size:
4087 1438 751 141 68
BsiEI CG_ry'CG
Cuts at: 104 3479 4027 4073 5281 104
Size: 3375 548 46 1208 1308
Fragments arranged by size:
3375 1308 1208 548 46
BsiHKAI G_wGCw'C
Cuts at: 1286 3499 4277 4467 5259 1286
Size: 2213 778 190 792 2512
Fragments arranged by size:
2512 2213 792 778 190
BsmI GAATG_Cn'
Cuts at: 769 829 1458 2184 769
Size: 60 629 726 5070
Fragments arranged by size:
5070 726 629 60
BsmBI CGTCTCn'nnnn_
Cuts at: 1701 2863 1701
Size: 1162 5323
Bsp1286I G_dGCh'C
Cuts at: 269 605 1121 1286 3499 4110 4203 4277 4467
Size: 336 516 165 2213 611 93 74 190
Cuts at: 4467 4529 5259 269
Size: 62 730 1495
Fragments arranged by size:
2213 1495 730 611 516 336 190 165
93 74 62
BspEI T'CCGG_A
Cuts at: 731 5705 731
Size: 4974 1511
BspHI T'CATG_A
Cuts at: 2286 5661 2286
Size: 3375 3110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 244 647 2389 3535 3749 4051 4432 244
Size: 403 1742 1146 214 302 381 2297
Fragments arranged by size:
2297 1742 1146 403 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4777 4831 6315 6480 50
Size: 4727 54 1484 165 55
Fragments arranged by size:
4727 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 239 1008 2888 4397 239
Size: 769 1880 1509 2327
Fragments arranged by size:
2327 1880 1509 769
BsrFI r'CCGG_y
Cuts at: 4483 4664 4873 4483
Size: 181 209 6095
Fragments arranged by size:
6095 209 181
BsrGI T'GTAC_A
Cuts at: 3149 3149
Size: 6485
BssHII G'CGCG_C
Cuts at: 2629 2629
Size: 6485
BssSI C'ACGA_G
Cuts at: 4756 5114 4756
Size: 358 6127
BstAPI GCAn_nnn'nTGC
Cuts at: 3862 3862
Size: 6485
BstBI TT'CG_AA
Cuts at: 4846 4846
Size: 6485
BstEII G'GTnAC_C
Cuts at: 2295 2295
Size: 6485
BstXI CCAn_nnnn'nTGG
Cuts at: 664 3116 664
Size: 2452 4033
BstYI r'GATC_y
Cuts at: 1 315 1346 1989 2541 2997 3510 3977 4000
Size: 314 1031 643 552 456 513 467 23
Cuts at: 4000 4335 4581 5593 5679 5691 6037 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1031 1012 643 552 513 467 456 449
346 335 314 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 176 2826 107
Size: 69 2650 3766
Fragments arranged by size:
3766 2650 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4435 4616 4779 4435
Size: 181 163 6141
Fragments arranged by size:
6141 181 163
BtsI GCAGTG_nn'
Cuts at: 572 918 1003 1386 1994 3728 572
Size: 346 85 383 608 1734 3329
Fragments arranged by size:
3329 1734 608 383 346 85
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1145 1180 1145
Size: 35 6450
DraI TTT'AAA
Cuts at: 3484 3484
Size: 6485
DrdI GACnn_nn'nnGTC
Cuts at: 4191 5049 4191
Size: 858 5627
EaeI y'GGCC_r
Cuts at: 677 1231 2613 2807 3122 3476 4070 4244 4635
Size: 554 1382 194 315 354 594 174 391
Cuts at: 4635 4662 6007 677
Size: 27 1345 1155
Fragments arranged by size:
1382 1345 1155 594 554 391 354 315
194 174 27
EagI C'GGCC_G
Cuts at: 3476 4070 3476
Size: 594 5891
EarI CTCTTCn'nnn_
Cuts at: 367 706 2419 4508 4718 367
Size: 339 1713 2089 210 2134
Fragments arranged by size:
2134 2089 1713 339 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4693 5003 5149 6295 4693
Size: 310 146 1146 4883
Fragments arranged by size:
4883 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3497 3497
Size: 6485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 839 1088 1105 2887 3298 3488 4310 4742
Size: 153 249 17 1782 411 190 822 432
Cuts at: 4742 5489 5757 686
Size: 747 268 1414
Fragments arranged by size:
1782 1414 822 747 432 411 268 249
190 153 17
EcoNI CCTnn'n_nnAGG
Cuts at: 717 1361 1745 717
Size: 644 384 5457
Fragments arranged by size:
5457 644 384
EcoO109I rG'GnC_Cy
Cuts at: 757 2208 3624 757
Size: 1451 1416 3618
Fragments arranged by size:
3618 1451 1416
EcoRI G'AATT_C
Cuts at: 3489 3489
Size: 6485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4504 4504
Size: 6485
FspI TGC'GCA
Cuts at: 4266 4266
Size: 6485
HaeII r_GCGC'y
Cuts at: 258 3919 4167 5189 6168 258
Size: 3661 248 1022 979 575
Fragments arranged by size:
3661 1022 979 575 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 422 454 1062 1094 1303 1335 1358 1390 1834
Size: 32 608 32 209 32 23 32 444
Cuts at: 1834 1866 1940 1972 1974 2006 3157 3189 3340
Size: 32 74 32 2 32 1151 32 151
Cuts at: 3340 3372 4327 4357 4359 4389 422
Size: 32 955 30 2 30 2518
Fragments arranged by size:
2518 1151 955 608 444 209 151 74
32 32 32 32 32 32 32 32
32 30 30 23 2 2
HincII GTy'rAC
Cuts at: 466 1959 3524 466
Size: 1493 1565 3427
Fragments arranged by size:
3427 1565 1493
HindIII A'AGCT_T
Cuts at: 470 1042 1220 1546 470
Size: 572 178 326 5409
Fragments arranged by size:
5409 572 326 178
KasI G'GCGC_C
Cuts at: 254 3915 4163 254
Size: 3661 248 2576
Fragments arranged by size:
3661 2576 248
KpnI G_GTAC'C
Cuts at: 3505 3505
Size: 6485
MfeI C'AATT_G
Cuts at: 2419 2419
Size: 6485
MluI A'CGCG_T
Cuts at: 5918 5918
Size: 6485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2075 5156 5340 2075
Size: 3081 184 3220
Fragments arranged by size:
3220 3081 184
MscI TGG'CCA
Cuts at: 2615 2809 3124 4246 2615
Size: 194 315 1122 4854
Fragments arranged by size:
4854 1122 315 194
MslI CAynn'nnrTG
Cuts at: 457 2162 4474 4601 457
Size: 1705 2312 127 2341
Fragments arranged by size:
2341 2312 1705 127
MspA1I CmG'CkG
Cuts at: 321 1055 2071 2323 2485 3596 3910 4270 5283
Size: 734 1016 252 162 1111 314 360 1013
Cuts at: 5283 5528 321
Size: 245 1278
Fragments arranged by size:
1278 1111 1016 1013 734 360 314 252
245 162
NaeI GCC'GGC
Cuts at: 4666 4666
Size: 6485
NarI GG'CG_CC
Cuts at: 255 3916 4164 255
Size: 3661 248 2576
Fragments arranged by size:
3661 2576 248
NcoI C'CATG_G
Cuts at: 107 176 2826 107
Size: 69 2650 3766
Fragments arranged by size:
3766 2650 69
NgoMIV G'CCGG_C
Cuts at: 4664 4664
Size: 6485
NsiI A_TGCA'T
Cuts at: 829 829
Size: 6485
NspI r_CATG'y
Cuts at: 1013 2783 3538 4038 4945 1013
Size: 1770 755 500 907 2553
Fragments arranged by size:
2553 1770 907 755 500
PasI CC'CwG_GG
Cuts at: 1928 1971 3264 1928
Size: 43 1293 5149
Fragments arranged by size:
5149 1293 43
PciI A'CATG_T
Cuts at: 4941 4941
Size: 6485
PflMI CCAn_nnn'nTGG
Cuts at: 350 983 3372 350
Size: 633 2389 3463
Fragments arranged by size:
3463 2389 633
PfoI T'CCnGG_A
Cuts at: 1104 1695 1104
Size: 591 5894
PmeI GTTT'AAAC
Cuts at: 3484 3484
Size: 6485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5624 5656 5624
Size: 32 6453
PpuMI rG'GwC_Cy
Cuts at: 757 757
Size: 6485
PstI C_TGCA'G
Cuts at: 998 1381 3532 4217 998
Size: 383 2151 685 3266
Fragments arranged by size:
3266 2151 685 383
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6485
PvuII CAG'CTG
Cuts at: 1055 2071 2485 3910 4270 1055
Size: 1016 414 1425 360 3270
Fragments arranged by size:
3270 1425 1016 414 360
RsrII CG'GwC_CG
Cuts at: 4680 4680
Size: 6485
SacI G_AGCT'C
Cuts at: 3499 3499
Size: 6485
SalI G'TCGA_C
Cuts at: 3522 3522
Size: 6485
SapI GCTCTTCn'nnn_
Cuts at: 4508 4718 4508
Size: 210 6275
SbfI CC_TGCA'GG
Cuts at: 3532 3532
Size: 6485
ScaI AGT'ACT
Cuts at: 6002 6002
Size: 6485
SexAI A'CCwGG_T
Cuts at: 2231 2231
Size: 6485
SfcI C'TryA_G
Cuts at: 33 994 1377 3270 3528 4213 5206 5397 33
Size: 961 383 1893 258 685 993 191 1121
Fragments arranged by size:
1893 1121 993 961 685 383 258 191
SfoI GGC'GCC
Cuts at: 256 3917 4165 256
Size: 3661 248 2576
Fragments arranged by size:
3661 2576 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6485
SmaI CCC'GGG
Cuts at: 2956 3507 2956
Size: 551 5934
SmlI C'TyrA_G
Cuts at: 332 2203 2312 2623 2970 3640 4555 5047 5309
Size: 1871 109 311 347 670 915 492 262
Cuts at: 5309 332
Size: 1508
Fragments arranged by size:
1871 1508 915 670 492 347 311 262
109
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6485
SphI G_CATG'C
Cuts at: 1013 3538 4038 1013
Size: 2525 500 3460
Fragments arranged by size:
3460 2525 500
SspI AAT'ATT
Cuts at: 1805 1878 2090 1805
Size: 73 212 6200
Fragments arranged by size:
6200 212 73
StyI C'CwwG_G
Cuts at: 107 176 1086 1964 2361 2826 3619 3973 107
Size: 69 910 878 397 465 793 354 2619
Fragments arranged by size:
2619 910 878 793 465 397 354 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 239 953 4068 4201 4660 4869 239
Size: 714 3115 133 459 209 1855
Fragments arranged by size:
3115 1855 714 459 209 133
TatI w'GTAC_w
Cuts at: 633 671 778 3149 6000 633
Size: 38 107 2371 2851 1118
Fragments arranged by size:
2851 2371 1118 107 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 506 538 1995 2027 3222 3254 6002 6034 506
Size: 32 1457 32 1195 32 2748 32 957
Fragments arranged by size:
2748 1457 1195 957 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4282 4282
Size: 6485
XbaI T'CTAG_A
Cuts at: 627 3516 627
Size: 2889 3596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 814 2564 3980 814
Size: 1750 1416 3319
Fragments arranged by size:
3319 1750 1416
XmaI C'CCGG_G
Cuts at: 2954 3505 2954
Size: 551 5934
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BmtI BsiWI BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI PacI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI