Restriction map for
pF1KB3043
(Circular) (Six-base) MAPSORT of: pF1KB3043.seq Check: 4806 from: 1 to: 6569
pF1KB3043 6569 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1904 2762 2882 3607 1904
Size: 858 120 725 4866
Fragments arranged by size:
4866 858 725 120
Acc65I G'GTAC_C
Cuts at: 3585 3585
Size: 6569
AclI AA'CG_TT
Cuts at: 6409 6409
Size: 6569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 707 1090 1155 2442 4394 4826 5573 5841
Size: 493 383 65 1287 1952 432 747 268
Cuts at: 5841 214
Size: 942
Fragments arranged by size:
1952 1287 942 747 493 432 383 268
65
AfeI AGC'GCT
Cuts at: 1176 2620 3115 1176
Size: 1444 495 4630
Fragments arranged by size:
4630 1444 495
AflIII A'CryG_T
Cuts at: 365 455 1040 3921 5025 6002 365
Size: 90 585 2881 1104 977 932
Fragments arranged by size:
2881 1104 977 932 585 90
AgeI A'CCGG_T
Cuts at: 4957 4957
Size: 6569
AhdI GACnn_n'nnGTC
Cuts at: 146 146
Size: 6569
AleI CACnn'nnGTG
Cuts at: 460 2668 460
Size: 2208 4361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1640 1640
Size: 6569
AlwNI CAG_nnn'CTG
Cuts at: 414 1789 1866 2066 2521 3874 5441 414
Size: 1375 77 200 455 1353 1567 1542
Fragments arranged by size:
1567 1542 1375 1353 455 200 77
ApaI G_GGCC'C
Cuts at: 1545 1734 2498 1545
Size: 189 764 5616
Fragments arranged by size:
5616 764 189
ApaLI G'TGCA_C
Cuts at: 433 5339 433
Size: 4906 1663
ApoI r'AATT_y
Cuts at: 691 2848 3232 3573 5922 6178 691
Size: 2157 384 341 2349 256 1082
Fragments arranged by size:
2349 2157 1082 384 341 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6569
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6569
AvaI C'yCGr_G
Cuts at: 1565 2498 3090 3546 3589 1565
Size: 933 592 456 43 4545
Fragments arranged by size:
4545 933 592 456 43
BamHI G'GATC_C
Cuts at: 235 487 3594 235
Size: 252 3107 3210
Fragments arranged by size:
3210 3107 252
BanI G'GyrC_C
Cuts at: 1374 1456 1468 2636 3024 3585 3999 4247 4282
Size: 82 12 1168 388 561 414 248 35
Cuts at: 4282 1374
Size: 3661
Fragments arranged by size:
3661 1168 561 414 388 248 82 35
12
BanII G_rGCy'C
Cuts at: 258 332 473 843 1545 1611 1734 1923 2290
Size: 74 141 370 702 66 123 189 367
Cuts at: 2290 2498 3583 4613 258
Size: 208 1085 1030 2214
Fragments arranged by size:
2214 1085 1030 702 370 367 208 189
141 123 74 66
BbeI G_GCGC'C
Cuts at: 1472 2640 4003 4251 1472
Size: 1168 1363 248 3790
Fragments arranged by size:
3790 1363 1168 248
BbsI GAAGACnn'nnnn_
Cuts at: 1078 2169 1078
Size: 1091 5478
BbvCI CC'TCA_GC
Cuts at: 407 2446 407
Size: 2039 4530
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1446 1480 3429 3463 1446
Size: 34 1949 34 4552
Fragments arranged by size:
4552 1949 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4467 5228 6569 4467
Size: 761 1341 4467
Fragments arranged by size:
4467 1341 761
BclI T'GATC_A
Cuts at: 1035 2025 4089 1035
Size: 990 2064 3515
Fragments arranged by size:
3515 2064 990
BglI GCCn_nnn'nGGC
Cuts at: 1729 3033 1729
Size: 1304 5265
BglII A'GATC_T
Cuts at: 1100 3320 4084 6121 1100
Size: 2220 764 2037 1548
Fragments arranged by size:
2220 2037 1548 764
BlpI GC'TnA_GC
Cuts at: 1204 3143 3681 1204
Size: 1939 538 4092
Fragments arranged by size:
4092 1939 538
Bme1580I G_kGCm'C
Cuts at: 437 1377 1459 1545 1591 1734 1931 2498 3029
Size: 940 82 86 46 143 197 567 531
Cuts at: 3029 4194 4287 5343 437
Size: 1165 93 1056 1663
Fragments arranged by size:
1663 1165 1056 940 567 531 197 143
93 86 82 46
BmgBI CAC'GTC
Cuts at: 1503 1719 1503
Size: 216 6353
BmrI ACTGGGnnnn_n'
Cuts at: 1236 2275 3781 3862 4197 6341 1236
Size: 1039 1506 81 335 2144 1464
Fragments arranged by size:
2144 1506 1464 1039 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 642 2086 2673 3105 4936 642
Size: 1444 587 432 1831 2275
Fragments arranged by size:
2275 1831 1444 587 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2593 2878 1831
Size: 762 285 5522
Fragments arranged by size:
5522 762 285
Bpu10I CC'TnA_GC
Cuts at: 407 1942 2446 407
Size: 1535 504 4530
Fragments arranged by size:
4530 1535 504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 2782 3745 4624 5116 5414 527
Size: 2255 963 879 492 298 1682
Fragments arranged by size:
2255 1682 963 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2236 2913 3382 6273 2236
Size: 677 469 2891 2532
Fragments arranged by size:
2891 2532 677 469
BsaAI yAC'GTr
Cuts at: 458 1041 1932 3098 3922 458
Size: 583 891 1166 824 3105
Fragments arranged by size:
3105 1166 891 824 583
BsaBI GATnn'nnATC
Cuts at: 6 4083 6120 6
Size: 4077 2037 455
Fragments arranged by size:
4077 2037 455
BsaHI Gr'CG_yC
Cuts at: 1469 2637 4000 4248 4950 6446 1469
Size: 1168 1363 248 702 1496 1592
Fragments arranged by size:
1592 1496 1363 1168 702 248
BsaWI w'CCGG_w
Cuts at: 695 2591 3758 4279 4957 5231 5378 5789 695
Size: 1896 1167 521 678 274 147 411 1475
Fragments arranged by size:
1896 1475 1167 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3446 3476 3446
Size: 30 6539
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1756 2649 2785 3009 1756
Size: 893 136 224 5316
Fragments arranged by size:
5316 893 224 136
BseYI C'CCAG_C
Cuts at: 1686 1949 3994 4752 5329 5825 1686
Size: 263 2045 758 577 496 2430
Fragments arranged by size:
2430 2045 758 577 496 263
BsiEI CG_ry'CG
Cuts at: 104 1112 2157 4111 4157 5365 104
Size: 1008 1045 1954 46 1208 1308
Fragments arranged by size:
1954 1308 1208 1045 1008 46
BsiHKAI G_wGCw'C
Cuts at: 437 1210 2089 2692 3313 3583 4361 4551 5343
Size: 773 879 603 621 270 778 190 792
Cuts at: 5343 437
Size: 1663
Fragments arranged by size:
1663 879 792 778 773 621 603 270
190
BsmI GAATG_Cn'
Cuts at: 855 855
Size: 6569
Bsp1286I G_dGCh'C
Cuts at: 258 332 437 473 843 1210 1377 1459 1545
Size: 74 105 36 370 367 167 82 86
Cuts at: 1545 1591 1611 1734 1923 1931 2089 2290 2498
Size: 46 20 123 189 8 158 201 208
Cuts at: 2498 2692 3029 3313 3583 4194 4287 4361 4551
Size: 194 337 284 270 611 93 74 190
Cuts at: 4551 4613 5343 258
Size: 62 730 1484
Fragments arranged by size:
1484 730 611 370 367 337 284 270
208 201 194 190 189 167 158 123
105 93 86 82 74 74 62 46
36 20 8
BspEI T'CCGG_A
Cuts at: 695 5789 695
Size: 5094 1475
BspHI T'CATG_A
Cuts at: 2465 5745 2465
Size: 3280 3289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 333 3619 3833 4135 4516 333
Size: 3286 214 302 381 2386
Fragments arranged by size:
3286 2386 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3429 4861 4915 6399 6564 50
Size: 3379 1432 54 1484 165 55
Fragments arranged by size:
3379 1484 1432 165 55 54
BsrDI GCAATG_nn'
Cuts at: 185 557 2427 4481 185
Size: 372 1870 2054 2273
Fragments arranged by size:
2273 2054 1870 372
BsrFI r'CCGG_y
Cuts at: 2865 4567 4748 4957 2865
Size: 1702 181 209 4477
Fragments arranged by size:
4477 1702 209 181
BsrGI T'GTAC_A
Cuts at: 3488 3488
Size: 6569
BssSI C'ACGA_G
Cuts at: 1031 2482 4840 5198 1031
Size: 1451 2358 358 2402
Fragments arranged by size:
2402 2358 1451 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1935 2696 3946 1935
Size: 761 1250 4558
Fragments arranged by size:
4558 1250 761
BstBI TT'CG_AA
Cuts at: 4930 4930
Size: 6569
BstEII G'GTnAC_C
Cuts at: 2559 2736 2892 3069 2559
Size: 177 156 177 6059
Fragments arranged by size:
6059 177 177 156
BstXI CCAn_nnnn'nTGG
Cuts at: 246 1855 1998 3012 246
Size: 1609 143 1014 3803
Fragments arranged by size:
3803 1609 1014 143
BstYI r'GATC_y
Cuts at: 1 235 487 860 1100 1786 2114 3320 3594
Size: 234 252 373 240 686 328 1206 274
Cuts at: 3594 4061 4084 4419 4665 5677 5763 5775 6121
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6121 1
Size: 449
Fragments arranged by size:
1206 1012 686 467 449 373 346 335
328 274 252 246 240 234 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 926 1782 926
Size: 856 5713
BtgI C'CryG_G
Cuts at: 107 350 791 1496 2901 3161 107
Size: 243 441 705 1405 260 3515
Fragments arranged by size:
3515 1405 705 441 260 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 999 1420 3162 4519 4700 4863 999
Size: 421 1742 1357 181 163 2705
Fragments arranged by size:
2705 1742 1357 421 181 163
BtsI GCAGTG_nn'
Cuts at: 453 990 1624 3812 453
Size: 537 634 2188 3210
Fragments arranged by size:
3210 2188 634 537
DraI TTT'AAA
Cuts at: 3568 3568
Size: 6569
DraIII CAC_nnn'GTG
Cuts at: 1870 1870
Size: 6569
DrdI GACnn_nn'nnGTC
Cuts at: 4275 5133 4275
Size: 858 5711
EaeI y'GGCC_r
Cuts at: 788 1721 2154 2353 4154 4328 4719 4746 6091
Size: 933 433 199 1801 174 391 27 1345
Cuts at: 6091 788
Size: 1266
Fragments arranged by size:
1801 1345 1266 933 433 391 199 174
27
EagI C'GGCC_G
Cuts at: 2154 4154 2154
Size: 2000 4569
EarI CTCTTCn'nnn_
Cuts at: 2077 2389 2680 4592 4802 2077
Size: 312 291 1912 210 3844
Fragments arranged by size:
3844 1912 312 291 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 325 624 792 2380 4777 5087 5233 6379 325
Size: 299 168 1588 2397 310 146 1146 515
Fragments arranged by size:
2397 1588 1146 515 310 299 168 146
EcoICRI GAG'CTC
Cuts at: 3581 3581
Size: 6569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 707 1090 1155 1831 2442 2593 2878 4394
Size: 493 383 65 676 611 151 285 1516
Cuts at: 4394 4826 5573 5841 214
Size: 432 747 268 942
Fragments arranged by size:
1516 942 747 676 611 493 432 383
285 268 151 65
EcoNI CCTnn'n_nnAGG
Cuts at: 1596 1596
Size: 6569
EcoO109I rG'GnC_Cy
Cuts at: 345 525 1481 1541 1542 1730 1731 1881 1946
Size: 180 956 60 1 188 1 150 65
Cuts at: 1946 2277 2495 2678 3156 3377 3411 3541 3708
Size: 331 218 183 478 221 34 130 167
Cuts at: 3708 345
Size: 3206
Fragments arranged by size:
3206 956 478 331 221 218 188 183
180 167 150 130 65 60 34 1
1
EcoRI G'AATT_C
Cuts at: 691 2848 3232 3573 691
Size: 2157 384 341 3687
Fragments arranged by size:
3687 2157 384 341
EcoRV GAT'ATC
Cuts at: 1384 1384
Size: 6569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 870 3132 3516 4588 870
Size: 2262 384 1072 2851
Fragments arranged by size:
2851 2262 1072 384
FspI TGC'GCA
Cuts at: 4350 4350
Size: 6569
HaeII r_GCGC'y
Cuts at: 1178 1472 2622 2640 3117 4003 4251 5273 6252
Size: 294 1150 18 477 886 248 1022 979
Cuts at: 6252 1178
Size: 1495
Fragments arranged by size:
1495 1150 1022 979 886 477 294 248
18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 132 164 929 961 1778 1810 2749 2781 3446
Size: 32 765 32 817 32 939 32 665
Cuts at: 3446 3478 4411 4441 4443 4473 132
Size: 32 933 30 2 30 2228
Fragments arranged by size:
2228 939 933 817 765 665 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 131 3608 131
Size: 3477 3092
HpaI GTT'AAC
Cuts at: 131 131
Size: 6569
KasI G'GCGC_C
Cuts at: 1468 2636 3999 4247 1468
Size: 1168 1363 248 3790
Fragments arranged by size:
3790 1363 1168 248
KpnI G_GTAC'C
Cuts at: 3589 3589
Size: 6569
MluI A'CGCG_T
Cuts at: 6002 6002
Size: 6569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 231 515 1463 2877 3155 5240 5424 231
Size: 284 948 1414 278 2085 184 1376
Fragments arranged by size:
2085 1414 1376 948 284 278 184
MscI TGG'CCA
Cuts at: 1723 4330 1723
Size: 2607 3962
MslI CAynn'nnrTG
Cuts at: 460 964 1279 1426 2668 4558 4685 460
Size: 504 315 147 1242 1890 127 2344
Fragments arranged by size:
2344 1890 1242 504 315 147 127
MspA1I CmG'CkG
Cuts at: 411 768 1815 2696 3316 3680 3994 4354 5367
Size: 357 1047 881 620 364 314 360 1013
Cuts at: 5367 5612 411
Size: 245 1368
Fragments arranged by size:
1368 1047 1013 881 620 364 360 357
314 245
NaeI GCC'GGC
Cuts at: 4750 4750
Size: 6569
NarI GG'CG_CC
Cuts at: 1469 2637 4000 4248 1469
Size: 1168 1363 248 3790
Fragments arranged by size:
3790 1363 1168 248
NcoI C'CATG_G
Cuts at: 107 2901 107
Size: 2794 3775
NdeI CA'TA_TG
Cuts at: 3052 3052
Size: 6569
NgoMIV G'CCGG_C
Cuts at: 4748 4748
Size: 6569
NspI r_CATG'y
Cuts at: 369 1431 2829 3622 4122 5029 369
Size: 1062 1398 793 500 907 1909
Fragments arranged by size:
1909 1398 1062 907 793 500
PasI CC'CwG_GG
Cuts at: 372 1598 1655 1923 1991 372
Size: 1226 57 268 68 4950
Fragments arranged by size:
4950 1226 268 68 57
PciI A'CATG_T
Cuts at: 365 5025 365
Size: 4660 1909
PfoI T'CCnGG_A
Cuts at: 2720 3224 2720
Size: 504 6065
PmeI GTTT'AAAC
Cuts at: 3568 3568
Size: 6569
PmlI CAC'GTG
Cuts at: 1041 1932 1041
Size: 891 5678
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2637 5708 5740 2605
Size: 32 3071 32 3434
Fragments arranged by size:
3434 3071 32 32
PpuMI rG'GwC_Cy
Cuts at: 345 1881 1946 2277 3377 3411 3541 345
Size: 1536 65 331 1100 34 130 3373
Fragments arranged by size:
3373 1536 1100 331 130 65 34
PshAI GACnn'nnGTC
Cuts at: 2215 2557 2215
Size: 342 6227
PspOMI G'GGCC_C
Cuts at: 1541 1730 2494 1541
Size: 189 764 5616
Fragments arranged by size:
5616 764 189
PstI C_TGCA'G
Cuts at: 344 2701 3616 4301 344
Size: 2357 915 685 2612
Fragments arranged by size:
2612 2357 915 685
PvuI CG_AT'CG
Cuts at: 104 1112 104
Size: 1008 5561
PvuII CAG'CTG
Cuts at: 411 768 2696 3316 3994 4354 411
Size: 357 1928 620 678 360 2626
Fragments arranged by size:
2626 1928 678 620 360 357
RsrII CG'GwC_CG
Cuts at: 4764 4764
Size: 6569
SacI G_AGCT'C
Cuts at: 3583 3583
Size: 6569
SalI G'TCGA_C
Cuts at: 3606 3606
Size: 6569
SanDI GG'GwC_CC
Cuts at: 3541 3541
Size: 6569
SapI GCTCTTCn'nnn_
Cuts at: 2077 2680 4592 4802 2077
Size: 603 1912 210 3844
Fragments arranged by size:
3844 1912 603 210
SbfI CC_TGCA'GG
Cuts at: 344 3616 344
Size: 3272 3297
ScaI AGT'ACT
Cuts at: 682 1958 6086 682
Size: 1276 4128 1165
Fragments arranged by size:
4128 1276 1165
SexAI A'CCwGG_T
Cuts at: 1526 1526
Size: 6569
SfcI C'TryA_G
Cuts at: 33 309 340 1913 2254 2547 2697 3612 4297
Size: 276 31 1573 341 293 150 915 685
Cuts at: 4297 5290 5481 33
Size: 993 191 1121
Fragments arranged by size:
1573 1121 993 915 685 341 293 276
191 150 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 1729 1729
Size: 6569
SfoI GGC'GCC
Cuts at: 1470 2638 4001 4249 1470
Size: 1168 1363 248 3790
Fragments arranged by size:
3790 1363 1168 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6569
SmaI CCC'GGG
Cuts at: 2500 3548 3591 2500
Size: 1048 43 5478
Fragments arranged by size:
5478 1048 43
SmlI C'TyrA_G
Cuts at: 542 2797 3090 3724 4639 5131 5393 542
Size: 2255 293 634 915 492 262 1718
Fragments arranged by size:
2255 1718 915 634 492 293 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6569
SphI G_CATG'C
Cuts at: 3622 4122 3622
Size: 500 6069
StyI C'CwwG_G
Cuts at: 107 902 2778 2901 3107 3703 4057 107
Size: 795 1876 123 206 596 354 2619
Fragments arranged by size:
2619 1876 795 596 354 206 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1474 2479 4152 4285 4744 4953 1474
Size: 1005 1673 133 459 209 3090
Fragments arranged by size:
3090 1673 1005 459 209 133
TatI w'GTAC_w
Cuts at: 680 1956 3488 6084 680
Size: 1276 1532 2596 1165
Fragments arranged by size:
2596 1532 1276 1165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 227 259 6086 6118 227
Size: 32 5827 32 678
Fragments arranged by size:
5827 678 32 32
Tth111I GACn'n_nGTC
Cuts at: 1528 4366 1528
Size: 2838 3731
XbaI T'CTAG_A
Cuts at: 3600 3600
Size: 6569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4064 4064
Size: 6569
XhoI C'TCGA_G
Cuts at: 3090 3090
Size: 6569
XmaI C'CCGG_G
Cuts at: 2498 3546 3589 2498
Size: 1048 43 5478
Fragments arranged by size:
5478 1048 43
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AloI AscI AvrII BaeI BfrBI
BmtI BsgI BsiWI BsmBI BssHII BstZ17I ClaI CspCI
FseI FspAI HindIII MfeI NheI NotI NruI NsiI
PacI PflMI PsiI PspXI PsrI SacII SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI