Restriction map for
pF1KB3044
(Circular) (Six-base) MAPSORT of: pF1KB3044.seq Check: 702 from: 1 to: 4985
pF1KB3044 4985 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 492 624 1394 2023 492
Size: 132 770 629 3454
Fragments arranged by size:
3454 770 629 132
Acc65I G'GTAC_C
Cuts at: 2001 2001
Size: 4985
AclI AA'CG_TT
Cuts at: 1929 4825 1929
Size: 2896 2089
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 2810 3242 3595 4257 299
Size: 2511 432 353 662 1027
Fragments arranged by size:
2511 1027 662 432 353
AfeI AGC'GCT
Cuts at: 184 184
Size: 4985
AflII C'TTAA_G
Cuts at: 1000 1000
Size: 4985
AflIII A'CryG_T
Cuts at: 736 795 2337 4137 4418 736
Size: 59 1542 1800 281 1303
Fragments arranged by size:
1800 1542 1303 281 59
AhdI GACnn_n'nnGTC
Cuts at: 1477 1477
Size: 4985
AlwNI CAG_nnn'CTG
Cuts at: 2290 3728 2290
Size: 1438 3547
ApaLI G'TGCA_C
Cuts at: 3823 3823
Size: 4985
ApoI r'AATT_y
Cuts at: 1918 1989 4338 4594 1918
Size: 71 2349 256 2309
Fragments arranged by size:
2349 2309 256 71
AseI AT'TA_AT
Cuts at: 20 815 1058 20
Size: 795 243 3947
Fragments arranged by size:
3947 795 243
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4985
AvaI C'yCGr_G
Cuts at: 2005 2005
Size: 4985
BamHI G'GATC_C
Cuts at: 150 307 408 1431 2010 150
Size: 157 101 1023 579 3125
Fragments arranged by size:
3125 1023 579 157 101
BanI G'GyrC_C
Cuts at: 1872 2001 2415 2663 2698 1872
Size: 129 414 248 35 4159
Fragments arranged by size:
4159 414 248 129 35
BanII G_rGCy'C
Cuts at: 142 1999 3029 142
Size: 1857 1030 2098
Fragments arranged by size:
2098 1857 1030
BbeI G_GCGC'C
Cuts at: 2419 2667 2419
Size: 248 4737
BbsI GAAGACnn'nnnn_
Cuts at: 1235 1235
Size: 4985
BciVI GTATCCnnnnn_n'
Cuts at: 1922 2883 3939 4985 1922
Size: 961 1056 1046 1922
Fragments arranged by size:
1922 1056 1046 961
BclI T'GATC_A
Cuts at: 2505 2505
Size: 4985
BglII A'GATC_T
Cuts at: 2500 4537 2500
Size: 2037 2948
BlpI GC'TnA_GC
Cuts at: 2097 2097
Size: 4985
Bme1580I G_kGCm'C
Cuts at: 1875 2610 2703 3827 1875
Size: 735 93 1124 3033
Fragments arranged by size:
3033 1124 735 93
BmrI ACTGGGnnnn_n'
Cuts at: 1527 2197 2278 2613 4757 1527
Size: 670 81 335 2144 1755
Fragments arranged by size:
2144 1755 670 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3352 3352
Size: 4985
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 1193 1883 542
Size: 651 690 3644
Fragments arranged by size:
3644 690 651
Bpu10I CC'TnA_GC
Cuts at: 404 404
Size: 4985
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2161 3040 3754 4052 2161
Size: 879 714 298 3094
Fragments arranged by size:
3094 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4689 4689
Size: 4985
BsaAI yAC'GTr
Cuts at: 580 2338 580
Size: 1758 3227
BsaBI GATnn'nnATC
Cuts at: 6 413 471 477 2499 4536 6
Size: 407 58 6 2022 2037 455
Fragments arranged by size:
2037 2022 455 407 58 6
BsaHI Gr'CG_yC
Cuts at: 1463 2416 2664 3366 4862 1463
Size: 953 248 702 1496 1586
Fragments arranged by size:
1586 1496 953 702 248
BsaWI w'CCGG_w
Cuts at: 2174 2695 3373 3784 3931 4205 2174
Size: 521 678 411 147 274 2954
Fragments arranged by size:
2954 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 1712 160
Size: 1552 3433
BseYI C'CCAG_C
Cuts at: 2410 3168 3833 4241 2410
Size: 758 665 408 3154
Fragments arranged by size:
3154 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 1330
Size: 4985
BsiEI CG_ry'CG
Cuts at: 104 2527 2573 3803 104
Size: 2423 46 1230 1286
Fragments arranged by size:
2423 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 405 1722 1999 2777 2967 3827 405
Size: 1317 277 778 190 860 1563
Fragments arranged by size:
1563 1317 860 778 277 190
BsmBI CGTCTCn'nnnn_
Cuts at: 151 1613 151
Size: 1462 3523
Bsp1286I G_dGCh'C
Cuts at: 142 405 1722 1875 1999 2610 2703 2777 2967
Size: 263 1317 153 124 611 93 74 190
Cuts at: 2967 3029 3827 142
Size: 62 798 1300
Fragments arranged by size:
1317 1300 798 611 263 190 153 124
93 74 62
BspHI T'CATG_A
Cuts at: 1710 3417 1710
Size: 1707 3278
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2035 2249 2551 2932 2035
Size: 214 302 381 4088
Fragments arranged by size:
4088 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3277 3331 4815 4980 50
Size: 3227 54 1484 165 55
Fragments arranged by size:
3227 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 433 995 1518 2897 433
Size: 562 523 1379 2521
Fragments arranged by size:
2521 1379 562 523
BsrFI r'CCGG_y
Cuts at: 2983 3164 2983
Size: 181 4804
BssSI C'ACGA_G
Cuts at: 3256 3964 3256
Size: 708 4277
BstAPI GCAn_nnn'nTGC
Cuts at: 862 2362 862
Size: 1500 3485
BstBI TT'CG_AA
Cuts at: 1644 3346 1644
Size: 1702 3283
BstYI r'GATC_y
Cuts at: 1 150 307 408 472 1431 2010 2477 2500
Size: 149 157 101 64 959 579 467 23
Cuts at: 2500 2835 3081 3387 3399 3485 4537 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 959 579 467 449 335 306 246
157 149 101 86 64 23 12
BstZ17I GTA'TAC
Cuts at: 1395 1395
Size: 4985
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2935 3116 3279 2935
Size: 181 163 4641
Fragments arranged by size:
4641 181 163
BtsI GCAGTG_nn'
Cuts at: 1901 2228 1901
Size: 327 4658
DraI TTT'AAA
Cuts at: 831 852 1439 1984 831
Size: 21 587 545 3832
Fragments arranged by size:
3832 587 545 21
DraIII CAC_nnn'GTG
Cuts at: 1132 1132
Size: 4985
DrdI GACnn_nn'nnGTC
Cuts at: 2691 4035 2691
Size: 1344 3641
EaeI y'GGCC_r
Cuts at: 2570 2744 3135 3162 4507 2570
Size: 174 391 27 1345 3048
Fragments arranged by size:
3048 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2570 2570
Size: 4985
EarI CTCTTCn'nnn_
Cuts at: 558 925 1061 1236 1295 1395 3008 3218 558
Size: 367 136 175 59 100 1613 210 2325
Fragments arranged by size:
2325 1613 367 210 175 136 100 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1361 3193 3935 4081 4795 1361
Size: 1832 742 146 714 1551
Fragments arranged by size:
1832 1551 742 714 146
EcoICRI GAG'CTC
Cuts at: 1997 1997
Size: 4985
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 542 1193 1883 2810 3242 3595 4257 299
Size: 243 651 690 927 432 353 662 1027
Fragments arranged by size:
1027 927 690 662 651 432 353 243
EcoO109I rG'GnC_Cy
Cuts at: 192 1042 2124 192
Size: 850 1082 3053
Fragments arranged by size:
3053 1082 850
EcoRI G'AATT_C
Cuts at: 1989 1989
Size: 4985
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1111 3004 1111
Size: 1893 3092
FspI TGC'GCA
Cuts at: 2766 2766
Size: 4985
HaeII r_GCGC'y
Cuts at: 186 2419 2667 3897 4668 186
Size: 2233 248 1230 771 503
Fragments arranged by size:
2233 1230 771 503 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 180 296 328 658 690 1463 1495 2827
Size: 32 116 32 330 32 773 32 1332
Cuts at: 2827 2857 2859 2889 148
Size: 30 2 30 2244
Fragments arranged by size:
2244 1332 773 330 116 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 493 633 1503 2024 493
Size: 140 870 521 3454
Fragments arranged by size:
3454 870 521 140
KasI G'GCGC_C
Cuts at: 2415 2663 2415
Size: 248 4737
KpnI G_GTAC'C
Cuts at: 2005 2005
Size: 4985
MfeI C'AATT_G
Cuts at: 1356 1356
Size: 4985
MluI A'CGCG_T
Cuts at: 736 4418 736
Size: 3682 1303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 3744 3928 92
Size: 3652 184 1149
Fragments arranged by size:
3652 1149 184
MscI TGG'CCA
Cuts at: 2746 2746
Size: 4985
MslI CAynn'nnrTG
Cuts at: 1715 2974 3101 1715
Size: 1259 127 3599
Fragments arranged by size:
3599 1259 127
MspA1I CmG'CkG
Cuts at: 923 2096 2410 2770 3554 3799 923
Size: 1173 314 360 784 245 2109
Fragments arranged by size:
2109 1173 784 360 314 245
NaeI GCC'GGC
Cuts at: 3166 3166
Size: 4985
NarI GG'CG_CC
Cuts at: 2416 2664 2416
Size: 248 4737
NdeI CA'TA_TG
Cuts at: 1842 1842
Size: 4985
NgoMIV G'CCGG_C
Cuts at: 3164 3164
Size: 4985
NspI r_CATG'y
Cuts at: 799 2038 2538 4141 799
Size: 1239 500 1603 1643
Fragments arranged by size:
1643 1603 1239 500
PciI A'CATG_T
Cuts at: 795 4137 795
Size: 3342 1643
PmeI GTTT'AAAC
Cuts at: 1984 1984
Size: 4985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3463 3431
Size: 32 4953
PpuMI rG'GwC_Cy
Cuts at: 192 192
Size: 4985
PstI C_TGCA'G
Cuts at: 304 1962 2032 2717 304
Size: 1658 70 685 2572
Fragments arranged by size:
2572 1658 685 70
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4985
PvuII CAG'CTG
Cuts at: 923 2410 2770 923
Size: 1487 360 3138
Fragments arranged by size:
3138 1487 360
RsrII CG'GwC_CG
Cuts at: 3180 3180
Size: 4985
SacI G_AGCT'C
Cuts at: 1999 1999
Size: 4985
SalI G'TCGA_C
Cuts at: 491 2022 491
Size: 1531 3454
SapI GCTCTTCn'nnn_
Cuts at: 1236 3008 3218 1236
Size: 1772 210 3003
Fragments arranged by size:
3003 1772 210
SbfI CC_TGCA'GG
Cuts at: 2032 2032
Size: 4985
ScaI AGT'ACT
Cuts at: 649 4502 649
Size: 3853 1132
SfcI C'TryA_G
Cuts at: 33 300 640 1221 1529 1958 2028 2713 3681
Size: 267 340 581 308 429 70 685 968
Cuts at: 3681 3872 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 581 429 340 308 267
191 70
SfoI GGC'GCC
Cuts at: 2417 2665 2417
Size: 248 4737
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4985
SmaI CCC'GGG
Cuts at: 2007 2007
Size: 4985
SmlI C'TyrA_G
Cuts at: 1000 2140 3055 3769 4031 1000
Size: 1140 915 714 262 1954
Fragments arranged by size:
1954 1140 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4985
SphI G_CATG'C
Cuts at: 2038 2538 2038
Size: 500 4485
StyI C'CwwG_G
Cuts at: 1778 2119 2473 1778
Size: 341 354 4290
Fragments arranged by size:
4290 354 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 212 2568 2701 3160 3369 212
Size: 2356 133 459 209 1828
Fragments arranged by size:
2356 1828 459 209 133
TatI w'GTAC_w
Cuts at: 647 4500 647
Size: 3853 1132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4502 4534 4502
Size: 32 4953
Tth111I GACn'n_nGTC
Cuts at: 162 2782 162
Size: 2620 2365
XbaI T'CTAG_A
Cuts at: 2016 2016
Size: 4985
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1362 2480 1362
Size: 1118 3867
XmaI C'CCGG_G
Cuts at: 2005 2005
Size: 4985
XmnI GAAnn'nnTTC
Cuts at: 1538 1538
Size: 4985
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BspEI BsrGI BssHII BstEII BstXI
Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI