(Circular) (Six-base) MAPSORT of: pF1KB3046.seq Check: 3146 from: 1 to: 7634 pF1KB3046 7634 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3715 3715 Size: 7634 AccI GT'mk_AC Cuts at: 797 1574 4672 797 Size: 777 3098 3759 Fragments arranged by size: 3759 3098 777 Acc65I G'GTAC_C Cuts at: 365 658 4650 365 Size: 293 3992 3349 Fragments arranged by size: 3992 3349 293 AclI AA'CG_TT Cuts at: 7474 7474 Size: 7634 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 190 869 1721 1870 3445 4571 5459 5891 6638 Size: 679 852 149 1575 1126 888 432 747 Cuts at: 6638 6906 190 Size: 268 918 Fragments arranged by size: 1575 1126 918 888 852 747 679 432 268 149 AflIII A'CryG_T Cuts at: 2674 2711 3711 4986 6090 7067 2674 Size: 37 1000 1275 1104 977 3241 Fragments arranged by size: 3241 1275 1104 1000 977 37 AgeI A'CCGG_T Cuts at: 6022 6022 Size: 7634 AhdI GACnn_n'nnGTC Cuts at: 2712 2712 Size: 7634 AleI CACnn'nnGTG Cuts at: 784 2108 3970 4348 784 Size: 1324 1862 378 4070 Fragments arranged by size: 4070 1862 1324 378 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 4628 4628 Size: 7634 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2374 2406 2845 2877 2374 Size: 32 439 32 7131 Fragments arranged by size: 7131 439 32 32 AlwNI CAG_nnn'CTG Cuts at: 4939 6506 4939 Size: 1567 6067 ApaI G_GGCC'C Cuts at: 3648 3648 Size: 7634 ApaLI G'TGCA_C Cuts at: 853 6404 853 Size: 5551 2083 ApoI r'AATT_y Cuts at: 217 679 723 1447 1985 3100 3478 3577 3631 Size: 462 44 724 538 1115 378 99 54 Cuts at: 3631 4459 4638 6987 7243 217 Size: 828 179 2349 256 608 Fragments arranged by size: 2349 1115 828 724 608 538 462 378 256 179 99 54 44 AseI AT'TA_AT Cuts at: 20 20 Size: 7634 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7634 AvaI C'yCGr_G Cuts at: 1272 1382 3647 3735 4654 1272 Size: 110 2265 88 919 4252 Fragments arranged by size: 4252 2265 919 110 88 AvrII C'CTAG_G Cuts at: 2355 2355 Size: 7634 BamHI G'GATC_C Cuts at: 708 4659 708 Size: 3951 3683 BanI G'GyrC_C Cuts at: 365 658 1042 3438 4650 5064 5312 5347 365 Size: 293 384 2396 1212 414 248 35 2652 Fragments arranged by size: 2652 2396 1212 414 384 293 248 35 BanII G_rGCy'C Cuts at: 447 2810 2897 3648 4648 5678 447 Size: 2363 87 751 1000 1030 2403 Fragments arranged by size: 2403 2363 1030 1000 751 87 BbeI G_GCGC'C Cuts at: 5068 5316 5068 Size: 248 7386 BbsI GAAGACnn'nnnn_ Cuts at: 178 1769 2404 3160 178 Size: 1591 635 756 4652 Fragments arranged by size: 4652 1591 756 635 BbvCI CC'TCA_GC Cuts at: 734 1954 2920 3386 734 Size: 1220 966 466 4982 Fragments arranged by size: 4982 1220 966 466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 1814 1848 4549 4583 90 Size: 34 1690 34 2701 34 3141 Fragments arranged by size: 3141 2701 1690 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 1560 2400 3136 5532 6293 7634 1560 Size: 840 736 2396 761 1341 1560 Fragments arranged by size: 2396 1560 1341 840 761 736 BclI T'GATC_A Cuts at: 5154 5154 Size: 7634 BfrBI ATG'CAT Cuts at: 1224 1224 Size: 7634 BglI GCCn_nnn'nGGC Cuts at: 2201 2201 Size: 7634 BglII A'GATC_T Cuts at: 2640 5149 7186 2640 Size: 2509 2037 3088 Fragments arranged by size: 3088 2509 2037 BlpI GC'TnA_GC Cuts at: 4746 4746 Size: 7634 Bme1580I G_kGCm'C Cuts at: 857 3361 3648 5259 5352 6408 857 Size: 2504 287 1611 93 1056 2083 Fragments arranged by size: 2504 2083 1611 1056 287 93 BmrI ACTGGGnnnn_n' Cuts at: 481 1870 1912 3610 4342 4846 4927 5262 7406 Size: 1389 42 1698 732 504 81 335 2144 Cuts at: 7406 481 Size: 709 Fragments arranged by size: 2144 1698 1389 732 709 504 335 81 42 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1720 3411 3926 6001 1720 Size: 1691 515 2075 3353 Fragments arranged by size: 3353 2075 1691 515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1463 4145 1463 Size: 2682 4952 Bpu10I CC'TnA_GC Cuts at: 734 1917 1954 2920 3386 3434 734 Size: 1183 37 966 466 48 4934 Fragments arranged by size: 4934 1183 966 466 48 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1451 1690 3921 4349 4810 5689 6181 6479 1451 Size: 239 2231 428 461 879 492 298 2606 Fragments arranged by size: 2606 2231 879 492 461 428 298 239 BsaI GGTCTCn'nnnn_ Cuts at: 7338 7338 Size: 7634 BsaAI yAC'GTr Cuts at: 4987 4987 Size: 7634 BsaBI GATnn'nnATC Cuts at: 6 907 4222 5148 7185 6 Size: 901 3315 926 2037 455 Fragments arranged by size: 3315 2037 926 901 455 BsaHI Gr'CG_yC Cuts at: 5065 5313 6015 7511 5065 Size: 248 702 1496 5188 Fragments arranged by size: 5188 1496 702 248 BsaWI w'CCGG_w Cuts at: 4823 5344 6022 6296 6443 6854 4823 Size: 521 678 274 147 411 5603 Fragments arranged by size: 5603 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 836 866 982 1012 2845 2875 2908 2938 3974 Size: 30 116 30 1833 30 33 30 1036 Cuts at: 3974 4004 836 Size: 30 4466 Fragments arranged by size: 4466 1833 1036 116 33 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 857 2560 4507 857 Size: 1703 1947 3984 Fragments arranged by size: 3984 1947 1703 BseYI C'CCAG_C Cuts at: 930 2453 4381 5059 5817 6394 6890 930 Size: 1523 1928 678 758 577 496 1674 Fragments arranged by size: 1928 1674 1523 758 678 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 185 1270 4579 185 Size: 1085 3309 3240 Fragments arranged by size: 3309 3240 1085 BsiEI CG_ry'CG Cuts at: 104 5176 5222 6430 104 Size: 5072 46 1208 1308 Fragments arranged by size: 5072 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 447 857 1706 3193 3614 4648 5426 5616 6408 Size: 410 849 1487 421 1034 778 190 792 Cuts at: 6408 447 Size: 1673 Fragments arranged by size: 1673 1487 1034 849 792 778 421 410 190 BsmI GAATG_Cn' Cuts at: 635 2221 2280 2332 635 Size: 1586 59 52 5937 Fragments arranged by size: 5937 1586 59 52 BsmBI CGTCTCn'nnnn_ Cuts at: 2163 2611 4367 2163 Size: 448 1756 5430 Fragments arranged by size: 5430 1756 448 Bsp1286I G_dGCh'C Cuts at: 447 857 1706 2810 2897 3193 3361 3614 3648 Size: 410 849 1104 87 296 168 253 34 Cuts at: 3648 4648 5259 5352 5426 5616 5678 6408 447 Size: 1000 611 93 74 190 62 730 1673 Fragments arranged by size: 1673 1104 1000 849 730 611 410 296 253 190 168 93 87 74 62 34 BspEI T'CCGG_A Cuts at: 6854 6854 Size: 7634 BspHI T'CATG_A Cuts at: 6810 6810 Size: 7634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 3715 4684 4898 5200 5581 3715 Size: 969 214 302 381 5768 Fragments arranged by size: 5768 969 381 302 214 BsrBI CCG'CTC Cuts at: 50 5926 5980 7464 7629 50 Size: 5876 54 1484 165 55 Fragments arranged by size: 5876 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 586 1523 2336 2687 4181 5546 586 Size: 937 813 351 1494 1365 2674 Fragments arranged by size: 2674 1494 1365 937 813 351 BsrFI r'CCGG_y Cuts at: 5632 5813 6022 5632 Size: 181 209 7244 Fragments arranged by size: 7244 209 181 BssSI C'ACGA_G Cuts at: 5905 6263 5905 Size: 358 7276 BstAPI GCAn_nnn'nTGC Cuts at: 1375 2338 2350 5011 1375 Size: 963 12 2661 3998 Fragments arranged by size: 3998 2661 963 12 BstBI TT'CG_AA Cuts at: 5995 5995 Size: 7634 BstEII G'GTnAC_C Cuts at: 1266 2436 4533 1266 Size: 1170 2097 4367 Fragments arranged by size: 4367 2097 1170 BstXI CCAn_nnnn'nTGG Cuts at: 786 1548 4350 4355 786 Size: 762 2802 5 4065 Fragments arranged by size: 4065 2802 762 5 BstYI r'GATC_y Cuts at: 1 708 1462 2457 2640 4659 5126 5149 5484 Size: 707 754 995 183 2019 467 23 335 Cuts at: 5484 5730 6742 6828 6840 7186 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 2019 1012 995 754 707 467 449 346 335 246 183 86 23 12 BtgI C'CryG_G Cuts at: 1064 2093 3048 4298 1064 Size: 1029 955 1250 4400 Fragments arranged by size: 4400 1250 1029 955 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 5584 5765 5928 5584 Size: 181 163 7290 Fragments arranged by size: 7290 181 163 BtsI GCAGTG_nn' Cuts at: 1832 3099 3133 4877 1832 Size: 1267 34 1744 4589 Fragments arranged by size: 4589 1744 1267 34 ClaI AT'CG_AT Cuts at: 1834 1834 Size: 7634 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 3473 3508 3473 Size: 35 7599 DraI TTT'AAA Cuts at: 2783 4633 2783 Size: 1850 5784 DraIII CAC_nnn'GTG Cuts at: 1906 3618 1906 Size: 1712 5922 DrdI GACnn_nn'nnGTC Cuts at: 2059 5340 6198 2059 Size: 3281 858 3495 Fragments arranged by size: 3495 3281 858 EaeI y'GGCC_r Cuts at: 312 416 1067 1656 2626 3962 4240 4357 5219 Size: 104 651 589 970 1336 278 117 862 Cuts at: 5219 5393 5784 5811 7156 312 Size: 174 391 27 1345 790 Fragments arranged by size: 1345 1336 970 862 790 651 589 391 278 174 117 104 27 EagI C'GGCC_G Cuts at: 5219 5219 Size: 7634 EarI CTCTTCn'nnn_ Cuts at: 3221 3581 4395 5657 5867 3221 Size: 360 814 1262 210 4988 Fragments arranged by size: 4988 1262 814 360 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 814 1881 5842 6152 6298 7444 814 Size: 1067 3961 310 146 1146 1004 Fragments arranged by size: 3961 1146 1067 1004 310 146 EcoICRI GAG'CTC Cuts at: 445 4646 445 Size: 4201 3433 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 190 869 1463 1721 1870 3445 4145 4571 5459 Size: 679 594 258 149 1575 700 426 888 Cuts at: 5459 5891 6638 6906 190 Size: 432 747 268 918 Fragments arranged by size: 1575 918 888 747 700 679 594 432 426 268 258 149 EcoNI CCTnn'n_nnAGG Cuts at: 3586 3586 Size: 7634 EcoO109I rG'GnC_Cy Cuts at: 3644 4773 3644 Size: 1129 6505 EcoRI G'AATT_C Cuts at: 4638 4638 Size: 7634 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5653 5653 Size: 7634 FspI TGC'GCA Cuts at: 5415 5415 Size: 7634 HaeII r_GCGC'y Cuts at: 5068 5316 6338 7317 5068 Size: 248 1022 979 5385 Fragments arranged by size: 5385 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 218 250 392 424 2698 2730 5476 5506 5508 Size: 32 142 32 2274 32 2746 30 2 Cuts at: 5508 5538 218 Size: 30 2314 Fragments arranged by size: 2746 2314 2274 142 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1197 3142 3948 4673 1197 Size: 1945 806 725 4158 Fragments arranged by size: 4158 1945 806 725 HindIII A'AGCT_T Cuts at: 2561 2561 Size: 7634 KasI G'GCGC_C Cuts at: 5064 5312 5064 Size: 248 7386 KpnI G_GTAC'C Cuts at: 369 662 4654 369 Size: 293 3992 3349 Fragments arranged by size: 3992 3349 293 MfeI C'AATT_G Cuts at: 2055 2055 Size: 7634 MluI A'CGCG_T Cuts at: 2674 7067 2674 Size: 4393 3241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1993 3276 4033 6305 6489 1993 Size: 1283 757 2272 184 3138 Fragments arranged by size: 3138 2272 1283 757 184 MscI TGG'CCA Cuts at: 314 418 1069 1658 2628 3964 5395 314 Size: 104 651 589 970 1336 1431 2553 Fragments arranged by size: 2553 1431 1336 970 651 589 104 MslI CAynn'nnrTG Cuts at: 628 784 886 1142 2108 2269 3751 3958 3970 Size: 156 102 256 966 161 1482 207 12 Cuts at: 3970 4348 5623 5750 628 Size: 378 1275 127 2512 Fragments arranged by size: 2512 1482 1275 966 378 256 207 161 156 127 102 12 MspA1I CmG'CkG Cuts at: 1773 2453 3409 4495 4745 5059 5419 6432 6677 Size: 680 956 1086 250 314 360 1013 245 Cuts at: 6677 1773 Size: 2730 Fragments arranged by size: 2730 1086 1013 956 680 360 314 250 245 NaeI GCC'GGC Cuts at: 5815 5815 Size: 7634 NarI GG'CG_CC Cuts at: 5065 5313 5065 Size: 248 7386 NcoI C'CATG_G Cuts at: 1064 2093 3048 1064 Size: 1029 955 5650 Fragments arranged by size: 5650 1029 955 NgoMIV G'CCGG_C Cuts at: 5813 5813 Size: 7634 NsiI A_TGCA'T Cuts at: 1226 1226 Size: 7634 NspI r_CATG'y Cuts at: 2715 3275 3715 4687 5187 6094 2715 Size: 560 440 972 500 907 4255 Fragments arranged by size: 4255 972 907 560 500 440 PciI A'CATG_T Cuts at: 2711 3711 6090 2711 Size: 1000 2379 4255 Fragments arranged by size: 4255 2379 1000 PflMI CCAn_nnn'nTGG Cuts at: 558 1000 1989 558 Size: 442 989 6203 Fragments arranged by size: 6203 989 442 PfoI T'CCnGG_A Cuts at: 275 2392 275 Size: 2117 5517 PmeI GTTT'AAAC Cuts at: 4633 4633 Size: 7634 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2845 2877 6773 6805 2845 Size: 32 3896 32 3674 Fragments arranged by size: 3896 3674 32 32 PspOMI G'GGCC_C Cuts at: 3644 3644 Size: 7634 PstI C_TGCA'G Cuts at: 2976 3374 4681 5366 2976 Size: 398 1307 685 5244 Fragments arranged by size: 5244 1307 685 398 PvuI CG_AT'CG Cuts at: 104 104 Size: 7634 PvuII CAG'CTG Cuts at: 1773 3409 4495 5059 5419 1773 Size: 1636 1086 564 360 3988 Fragments arranged by size: 3988 1636 1086 564 360 RsrII CG'GwC_CG Cuts at: 5829 5829 Size: 7634 SacI G_AGCT'C Cuts at: 447 4648 447 Size: 4201 3433 SalI G'TCGA_C Cuts at: 4671 4671 Size: 7634 SapI GCTCTTCn'nnn_ Cuts at: 5657 5867 5657 Size: 210 7424 SbfI CC_TGCA'GG Cuts at: 4681 4681 Size: 7634 ScaI AGT'ACT Cuts at: 7151 7151 Size: 7634 SfcI C'TryA_G Cuts at: 33 586 794 2585 2972 3370 4599 4677 5362 Size: 553 208 1791 387 398 1229 78 685 Cuts at: 5362 6355 6546 33 Size: 993 191 1121 Fragments arranged by size: 1791 1229 1121 993 685 553 398 387 208 191 78 SfoI GGC'GCC Cuts at: 5066 5314 5066 Size: 248 7386 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7634 SmaI CCC'GGG Cuts at: 1274 4656 1274 Size: 3382 4252 SmlI C'TyrA_G Cuts at: 1466 1705 3900 4364 4789 5704 6196 6458 1466 Size: 239 2195 464 425 915 492 262 2642 Fragments arranged by size: 2642 2195 915 492 464 425 262 239 SpeI A'CTAG_T Cuts at: 67 1371 67 Size: 1304 6330 SphI G_CATG'C Cuts at: 4687 5187 4687 Size: 500 7134 SspI AAT'ATT Cuts at: 487 487 Size: 7634 StuI AGG'CCT Cuts at: 1966 3516 1966 Size: 1550 6084 StyI C'CwwG_G Cuts at: 746 1064 1148 2093 2355 2693 2889 2944 3048 Size: 318 84 945 262 338 196 55 104 Cuts at: 3048 3236 3442 3777 3968 4251 4768 5122 746 Size: 188 206 335 191 283 517 354 3258 Fragments arranged by size: 3258 945 517 354 338 335 318 283 262 206 196 191 188 104 84 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1274 1642 1894 2086 2200 2594 3629 5217 5350 Size: 368 252 192 114 394 1035 1588 133 Cuts at: 5350 5809 6018 1274 Size: 459 209 2890 Fragments arranged by size: 2890 1588 1035 459 394 368 252 209 192 133 114 TatI w'GTAC_w Cuts at: 1009 3218 4607 7149 1009 Size: 2209 1389 2542 1494 Fragments arranged by size: 2542 2209 1494 1389 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 257 289 836 868 2430 2462 3044 3076 7151 Size: 32 547 32 1562 32 582 32 4075 Cuts at: 7151 7183 257 Size: 32 708 Fragments arranged by size: 4075 1562 708 582 547 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 5431 5431 Size: 7634 XbaI T'CTAG_A Cuts at: 4665 4665 Size: 7634 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1986 4128 5129 1986 Size: 2142 1001 4491 Fragments arranged by size: 4491 2142 1001 XmaI C'CCGG_G Cuts at: 1272 4654 1272 Size: 3382 4252 XmnI GAAnn'nnTTC Cuts at: 599 2036 3577 4151 599 Size: 1437 1541 574 4082 Fragments arranged by size: 4082 1541 1437 574 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AscI BaeI BmgBI BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I EcoRV FseI FspAI HpaI NdeI NheI NotI NruI PacI PasI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI