Restriction map for
pF1KB3051
(Circular) (Six-base) MAPSORT of: pF1KB3051.seq Check: 513 from: 1 to: 6758
pF1KB3051 6758 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1517 2415 1517
Size: 898 5860
AatII G_ACGT'C
Cuts at: 431 799 431
Size: 368 6390
AccI GT'mk_AC
Cuts at: 1259 2734 3680 3796 1259
Size: 1475 946 116 4221
Fragments arranged by size:
4221 1475 946 116
Acc65I G'GTAC_C
Cuts at: 1273 2183 3774 1273
Size: 910 1591 4257
Fragments arranged by size:
4257 1591 910
AclI AA'CG_TT
Cuts at: 6598 6598
Size: 6758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 652 691 1120 1846 3390 3402 4583 5015
Size: 384 39 429 726 1544 12 1181 432
Cuts at: 5015 5762 6030 268
Size: 747 268 996
Fragments arranged by size:
1544 1181 996 747 726 432 429 384
268 39 12
AfeI AGC'GCT
Cuts at: 1899 1899
Size: 6758
AflIII A'CryG_T
Cuts at: 4110 5214 6191 4110
Size: 1104 977 4677
Fragments arranged by size:
4677 1104 977
AgeI A'CCGG_T
Cuts at: 5146 5146
Size: 6758
AhdI GACnn_n'nnGTC
Cuts at: 2010 2010
Size: 6758
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 748 780 748
Size: 32 6726
AlwNI CAG_nnn'CTG
Cuts at: 246 1152 2316 4063 5630 246
Size: 906 1164 1747 1567 1374
Fragments arranged by size:
1747 1567 1374 1164 906
ApaI G_GGCC'C
Cuts at: 1188 1188
Size: 6758
ApaLI G'TGCA_C
Cuts at: 1502 5528 1502
Size: 4026 2732
ApoI r'AATT_y
Cuts at: 3075 3762 6111 6367 3075
Size: 687 2349 256 3466
Fragments arranged by size:
3466 2349 687 256
AscI GG'CGCG_CC
Cuts at: 3020 3020
Size: 6758
AseI AT'TA_AT
Cuts at: 20 20
Size: 6758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6758
AvaI C'yCGr_G
Cuts at: 640 697 2196 3778 640
Size: 57 1499 1582 3620
Fragments arranged by size:
3620 1582 1499 57
BamHI G'GATC_C
Cuts at: 3783 3783
Size: 6758
BanI G'GyrC_C
Cuts at: 421 1273 2108 2183 2303 3774 4188 4436 4471
Size: 852 835 75 120 1471 414 248 35
Cuts at: 4471 421
Size: 2708
Fragments arranged by size:
2708 1471 852 835 414 248 120 75
35
BanII G_rGCy'C
Cuts at: 893 1188 2164 2511 2795 3191 3238 3505 3772
Size: 295 976 347 284 396 47 267 267
Cuts at: 3772 4802 893
Size: 1030 2849
Fragments arranged by size:
2849 1030 976 396 347 295 284 267
267 47
BbeI G_GCGC'C
Cuts at: 2112 4192 4440 2112
Size: 2080 248 4430
Fragments arranged by size:
4430 2080 248
BbsI GAAGACnn'nnnn_
Cuts at: 1108 2031 1108
Size: 923 5835
BbvCI CC'TCA_GC
Cuts at: 2711 2711
Size: 6758
BciVI GTATCCnnnnn_n'
Cuts at: 307 4656 5417 6758 307
Size: 4349 761 1341 307
Fragments arranged by size:
4349 1341 761 307
BclI T'GATC_A
Cuts at: 611 3437 4278 611
Size: 2826 841 3091
Fragments arranged by size:
3091 2826 841
BglI GCCn_nnn'nGGC
Cuts at: 1732 1936 3220 1732
Size: 204 1284 5270
Fragments arranged by size:
5270 1284 204
BglII A'GATC_T
Cuts at: 3554 4273 6310 3554
Size: 719 2037 4002
Fragments arranged by size:
4002 2037 719
BlpI GC'TnA_GC
Cuts at: 3190 3870 3190
Size: 680 6078
Bme1580I G_kGCm'C
Cuts at: 1188 1399 1506 4383 4476 5532 1188
Size: 211 107 2877 93 1056 2414
Fragments arranged by size:
2877 2414 1056 211 107 93
BmgBI CAC'GTC
Cuts at: 917 1437 1875 917
Size: 520 438 5800
Fragments arranged by size:
5800 520 438
BmrI ACTGGGnnnn_n'
Cuts at: 822 1776 1918 3294 3970 4051 4386 6530 822
Size: 954 142 1376 676 81 335 2144 1050
Fragments arranged by size:
2144 1376 1050 954 676 335 142 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2016 5125 2016
Size: 3109 3649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1411 1825 2308 2338 2963 3172 3499 703
Size: 708 414 483 30 625 209 327 3962
Fragments arranged by size:
3962 708 625 483 414 327 209 30
Bpu10I CC'TnA_GC
Cuts at: 1745 2535 2711 1745
Size: 790 176 5792
Fragments arranged by size:
5792 790 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 486 3160 3328 3513 3934 4813 5305 5603
Size: 386 2674 168 185 421 879 492 298
Cuts at: 5603 100
Size: 1255
Fragments arranged by size:
2674 1255 879 492 421 386 298 185
168
BsaI GGTCTCn'nnnn_
Cuts at: 536 2575 6462 536
Size: 2039 3887 832
Fragments arranged by size:
3887 2039 832
BsaAI yAC'GTr
Cuts at: 1257 4111 1257
Size: 2854 3904
BsaBI GATnn'nnATC
Cuts at: 6 1039 1231 4272 6309 6
Size: 1033 192 3041 2037 455
Fragments arranged by size:
3041 2037 1033 455 192
BsaHI Gr'CG_yC
Cuts at: 428 796 1822 1979 2061 2109 2837 3254 3284
Size: 368 1026 157 82 48 728 417 30
Cuts at: 3284 3461 4189 4437 5139 6635 428
Size: 177 728 248 702 1496 551
Fragments arranged by size:
1496 1026 728 728 702 551 417 368
248 177 157 82 48 30
BsaWI w'CCGG_w
Cuts at: 3947 4468 5146 5420 5567 5978 3947
Size: 521 678 274 147 411 4727
Fragments arranged by size:
4727 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1807 1837 3604 3634 1807
Size: 30 1767 30 4931
Fragments arranged by size:
4931 1767 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 922 1903 2062 2065 2305 2308 2346 2458 2875
Size: 981 159 3 240 3 38 112 417
Cuts at: 2875 2953 3004 3043 3046 3055 3228 922
Size: 78 51 39 3 9 173 4452
Fragments arranged by size:
4452 981 417 240 173 159 112 78
51 39 38 9 3 3 3
BseYI C'CCAG_C
Cuts at: 326 475 870 1526 1757 1909 2420 2467 4183
Size: 149 395 656 231 152 511 47 1716
Cuts at: 4183 4941 5518 6014 326
Size: 758 577 496 1070
Fragments arranged by size:
1716 1070 758 656 577 511 496 395
231 152 149 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1696 1810 1831 2242 3031 3397 3711 1141
Size: 555 114 21 411 789 366 314 4188
Fragments arranged by size:
4188 789 555 411 366 314 114 21
BsiEI CG_ry'CG
Cuts at: 104 141 2077 3229 4300 4346 5554 104
Size: 37 1936 1152 1071 46 1208 1308
Fragments arranged by size:
1936 1308 1208 1152 1071 46 37
BsiHKAI G_wGCw'C
Cuts at: 849 968 1506 2164 2210 2795 3191 3505 3772
Size: 119 538 658 46 585 396 314 267
Cuts at: 3772 4550 4740 5532 849
Size: 778 190 792 2075
Fragments arranged by size:
2075 792 778 658 585 538 396 314
267 190 119 46
BsmI GAATG_Cn'
Cuts at: 2463 3521 2463
Size: 1058 5700
BsmBI CGTCTCn'nnnn_
Cuts at: 3291 3291
Size: 6758
Bsp1286I G_dGCh'C
Cuts at: 849 893 968 1188 1399 1506 2164 2210 2511
Size: 44 75 220 211 107 658 46 301
Cuts at: 2511 2795 3191 3238 3505 3772 4383 4476 4550
Size: 284 396 47 267 267 611 93 74
Cuts at: 4550 4740 4802 5532 849
Size: 190 62 730 2075
Fragments arranged by size:
2075 730 658 611 396 301 284 267
267 220 211 190 107 93 75 74
62 47 46 44
BspEI T'CCGG_A
Cuts at: 5978 5978
Size: 6758
BspHI T'CATG_A
Cuts at: 560 5934 560
Size: 5374 1384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1202 1517 2415 3808 4022 4324 4705 1202
Size: 315 898 1393 214 302 381 3255
Fragments arranged by size:
3255 1393 898 381 315 302 214
BsrBI CCG'CTC
Cuts at: 50 777 1818 2298 2943 3620 5050 5104 6588
Size: 727 1041 480 645 677 1430 54 1484
Cuts at: 6588 6753 50
Size: 165 55
Fragments arranged by size:
1484 1430 1041 727 677 645 480 165
55 54
BsrDI GCAATG_nn'
Cuts at: 1091 3362 4670 1091
Size: 2271 1308 3179
Fragments arranged by size:
3179 2271 1308
BsrFI r'CCGG_y
Cuts at: 1772 2072 2306 3006 4756 4937 5146 1772
Size: 300 234 700 1750 181 209 3384
Fragments arranged by size:
3384 1750 700 300 234 209 181
BsrGI T'GTAC_A
Cuts at: 617 3611 617
Size: 2994 3764
BssHII G'CGCG_C
Cuts at: 3020 3020
Size: 6758
BssSI C'ACGA_G
Cuts at: 2044 3391 5029 5387 2044
Size: 1347 1638 358 3415
Fragments arranged by size:
3415 1638 1347 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4135 4135
Size: 6758
BstBI TT'CG_AA
Cuts at: 5119 5119
Size: 6758
BstXI CCAn_nnnn'nTGG
Cuts at: 546 3150 546
Size: 2604 4154
BstYI r'GATC_y
Cuts at: 1 452 1094 1538 1568 3554 3783 4250 4273
Size: 451 642 444 30 1986 229 467 23
Cuts at: 4273 4608 4854 5866 5952 5964 6310 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1986 1012 642 467 451 449 444 346
335 246 229 86 30 23 12
BtgI C'CryG_G
Cuts at: 107 174 865 2216 2390 2612 2783 107
Size: 67 691 1351 174 222 171 4082
Fragments arranged by size:
4082 1351 691 222 174 171 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 246 1401 2153 2394 4708 4889 5052 246
Size: 1155 752 241 2314 181 163 1952
Fragments arranged by size:
2314 1952 1155 752 241 181 163
BtsI GCAGTG_nn'
Cuts at: 968 1153 3699 4001 968
Size: 185 2546 302 3725
Fragments arranged by size:
3725 2546 302 185
DraI TTT'AAA
Cuts at: 3757 3757
Size: 6758
DrdI GACnn_nn'nnGTC
Cuts at: 510 570 2236 3130 4464 5322 510
Size: 60 1666 894 1334 858 1946
Fragments arranged by size:
1946 1666 1334 894 858 60
EaeI y'GGCC_r
Cuts at: 737 1407 1439 1514 2074 3140 3226 3743 4343
Size: 670 32 75 560 1066 86 517 600
Cuts at: 4343 4517 4908 4935 6280 737
Size: 174 391 27 1345 1215
Fragments arranged by size:
1345 1215 1066 670 600 560 517 391
174 86 75 32 27
EagI C'GGCC_G
Cuts at: 2074 3226 4343 2074
Size: 1152 1117 4489
Fragments arranged by size:
4489 1152 1117
EarI CTCTTCn'nnn_
Cuts at: 2090 2620 3719 4781 4991 2090
Size: 530 1099 1062 210 3857
Fragments arranged by size:
3857 1099 1062 530 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2101 4966 5276 5422 6568 2101
Size: 2865 310 146 1146 2291
Fragments arranged by size:
2865 2291 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2162 2793 3189 3503 3770 2162
Size: 631 396 314 267 5150
Fragments arranged by size:
5150 631 396 314 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 652 691 703 1120 1411 1825 1846 2308
Size: 384 39 12 417 291 414 21 462
Cuts at: 2308 2338 2963 3172 3390 3402 3499 4583 5015
Size: 30 625 209 218 12 97 1084 432
Cuts at: 5015 5762 6030 268
Size: 747 268 996
Fragments arranged by size:
1084 996 747 625 462 432 417 414
384 291 268 218 209 97 39 30
21 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 591 591
Size: 6758
EcoO109I rG'GnC_Cy
Cuts at: 687 816 1134 1185 2715 3350 3897 687
Size: 129 318 51 1530 635 547 3548
Fragments arranged by size:
3548 1530 635 547 318 129 51
EcoRI G'AATT_C
Cuts at: 3762 3762
Size: 6758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 390 3420 4777 390
Size: 3030 1357 2371
Fragments arranged by size:
3030 2371 1357
FspI TGC'GCA
Cuts at: 4539 4539
Size: 6758
HaeII r_GCGC'y
Cuts at: 1004 1745 1901 2112 2373 4192 4440 5462 6441
Size: 741 156 211 261 1819 248 1022 979
Cuts at: 6441 1004
Size: 1321
Fragments arranged by size:
1819 1321 1022 979 741 261 248 211
156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 305 337 432 464 1807 1839 1996 2028 2881
Size: 32 95 32 1343 32 157 32 853
Cuts at: 2881 2913 3429 3461 4600 4630 4632 4662 305
Size: 32 516 32 1139 30 2 30 2401
Fragments arranged by size:
2401 1343 1139 853 516 157 95 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3681 3797 3681
Size: 116 6642
HindIII A'AGCT_T
Cuts at: 1858 1858
Size: 6758
KasI G'GCGC_C
Cuts at: 2108 4188 4436 2108
Size: 2080 248 4430
Fragments arranged by size:
4430 2080 248
KpnI G_GTAC'C
Cuts at: 1277 2187 3778 1277
Size: 910 1591 4257
Fragments arranged by size:
4257 1591 910
MluI A'CGCG_T
Cuts at: 6191 6191
Size: 6758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1352 2060 3314 5429 5613 1352
Size: 708 1254 2115 184 2497
Fragments arranged by size:
2497 2115 1254 708 184
MscI TGG'CCA
Cuts at: 739 1441 1516 3142 3745 4519 739
Size: 702 75 1626 603 774 2978
Fragments arranged by size:
2978 1626 774 702 603 75
MslI CAynn'nnrTG
Cuts at: 265 349 1372 1447 2215 3328 3526 4747 4874
Size: 84 1023 75 768 1113 198 1221 127
Cuts at: 4874 265
Size: 2149
Fragments arranged by size:
2149 1221 1113 1023 768 198 127 84
75
MspA1I CmG'CkG
Cuts at: 176 326 867 1530 1839 2058 2998 3273 3422
Size: 150 541 663 309 219 940 275 149
Cuts at: 3422 3869 4183 4543 5556 5801 176
Size: 447 314 360 1013 245 1133
Fragments arranged by size:
1133 1013 940 663 541 447 360 314
309 275 245 219 150 149
NaeI GCC'GGC
Cuts at: 4939 4939
Size: 6758
NarI GG'CG_CC
Cuts at: 2109 4189 4437 2109
Size: 2080 248 4430
Fragments arranged by size:
4430 2080 248
NcoI C'CATG_G
Cuts at: 107 2612 107
Size: 2505 4253
NgoMIV G'CCGG_C
Cuts at: 4937 4937
Size: 6758
NspI r_CATG'y
Cuts at: 147 990 3811 4311 5218 147
Size: 843 2821 500 907 1687
Fragments arranged by size:
2821 1687 907 843 500
PasI CC'CwG_GG
Cuts at: 2030 2030
Size: 6758
PciI A'CATG_T
Cuts at: 5214 5214
Size: 6758
PflMI CCAn_nnn'nTGG
Cuts at: 812 1431 1614 1764 2427 812
Size: 619 183 150 663 5143
Fragments arranged by size:
5143 663 619 183 150
PmeI GTTT'AAAC
Cuts at: 3757 3757
Size: 6758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3658 3690 5897 5929 3658
Size: 32 2207 32 4487
Fragments arranged by size:
4487 2207 32 32
PpuMI rG'GwC_Cy
Cuts at: 687 816 1134 2715 3350 687
Size: 129 318 1581 635 4095
Fragments arranged by size:
4095 1581 635 318 129
PspOMI G'GGCC_C
Cuts at: 1184 1184
Size: 6758
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 610 642 1051 1083 610
Size: 32 409 32 6285
Fragments arranged by size:
6285 409 32 32
PstI C_TGCA'G
Cuts at: 164 1148 1321 1598 1712 1838 3119 3427 3805
Size: 984 173 277 114 126 1281 308 378
Cuts at: 3805 4490 164
Size: 685 2432
Fragments arranged by size:
2432 1281 984 685 378 308 277 173
126 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6758
PvuII CAG'CTG
Cuts at: 326 1839 3273 3422 4183 4543 326
Size: 1513 1434 149 761 360 2541
Fragments arranged by size:
2541 1513 1434 761 360 149
RsrII CG'GwC_CG
Cuts at: 4953 4953
Size: 6758
SacI G_AGCT'C
Cuts at: 2164 2795 3191 3505 3772 2164
Size: 631 396 314 267 5150
Fragments arranged by size:
5150 631 396 314 267
SacII CC_GC'GG
Cuts at: 177 868 177
Size: 691 6067
SalI G'TCGA_C
Cuts at: 3679 3795 3679
Size: 116 6642
SanDI GG'GwC_CC
Cuts at: 3350 3350
Size: 6758
SapI GCTCTTCn'nnn_
Cuts at: 2090 2620 4781 4991 2090
Size: 530 2161 210 3857
Fragments arranged by size:
3857 2161 530 210
SbfI CC_TGCA'GG
Cuts at: 3805 3805
Size: 6758
ScaI AGT'ACT
Cuts at: 6275 6275
Size: 6758
SfcI C'TryA_G
Cuts at: 33 160 1144 1317 1594 1708 1834 2502 3115
Size: 127 984 173 277 114 126 668 613
Cuts at: 3115 3423 3453 3801 4486 5479 5670 33
Size: 308 30 348 685 993 191 1121
Fragments arranged by size:
1121 993 984 685 668 613 348 308
277 191 173 127 126 114 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 1936 1936
Size: 6758
SfoI GGC'GCC
Cuts at: 2110 4190 4438 2110
Size: 2080 248 4430
Fragments arranged by size:
4430 2080 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6758
SmaI CCC'GGG
Cuts at: 699 2198 3780 699
Size: 1499 1582 3677
Fragments arranged by size:
3677 1582 1499
SmlI C'TyrA_G
Cuts at: 115 465 3175 3343 3492 3913 4828 5320 5582
Size: 350 2710 168 149 421 915 492 262
Cuts at: 5582 115
Size: 1291
Fragments arranged by size:
2710 1291 915 492 421 350 262 168
149
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6758
SphI G_CATG'C
Cuts at: 990 3811 4311 990
Size: 2821 500 3437
Fragments arranged by size:
3437 2821 500
StuI AGG'CCT
Cuts at: 1967 1967
Size: 6758
StyI C'CwwG_G
Cuts at: 107 1868 1934 2612 3892 4246 107
Size: 1761 66 678 1280 354 2619
Fragments arranged by size:
2619 1761 1280 678 354 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1540 1771 4341 4474 4933 5142 1540
Size: 231 2570 133 459 209 3156
Fragments arranged by size:
3156 2570 459 231 209 133
TatI w'GTAC_w
Cuts at: 617 1337 3611 6273 617
Size: 720 2274 2662 1102
Fragments arranged by size:
2662 2274 1102 720
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1099 1131 3559 3591 3678 3710 6275 6307 1099
Size: 32 2428 32 87 32 2565 32 1550
Fragments arranged by size:
2565 2428 1550 87 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 169 2434 3354 4555 169
Size: 2265 920 1201 2372
Fragments arranged by size:
2372 2265 1201 920
XbaI T'CTAG_A
Cuts at: 3789 3789
Size: 6758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4253 4253
Size: 6758
XmaI C'CCGG_G
Cuts at: 697 2196 3778 697
Size: 1499 1582 3677
Fragments arranged by size:
3677 1582 1499
ZraI GAC'GTC
Cuts at: 429 797 429
Size: 368 6390
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AleI AlfI AvrII BaeI BcgI BfrBI BmtI
BsiWI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PspXI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI