Restriction map for
pF1KB3065
(Circular) (Six-base) MAPSORT of: pF1KB3065.seq Check: 4218 from: 1 to: 4529
pF1KB3065 4529 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 620 1567 620
Size: 947 3582
Acc65I G'GTAC_C
Cuts at: 1545 1545
Size: 4529
AclI AA'CG_TT
Cuts at: 4369 4369
Size: 4529
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2786 3139 3801 2354
Size: 432 353 662 3082
Fragments arranged by size:
3082 662 432 353
AflIII A'CryG_T
Cuts at: 641 668 989 1067 1505 1881 3681 3962 641
Size: 27 321 78 438 376 1800 281 1208
Fragments arranged by size:
1800 1208 438 376 321 281 78 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1467 1467
Size: 4529
AlwNI CAG_nnn'CTG
Cuts at: 456 1371 1834 3272 456
Size: 915 463 1438 1713
Fragments arranged by size:
1713 1438 915 463
ApaI G_GGCC'C
Cuts at: 1180 1180
Size: 4529
ApaLI G'TGCA_C
Cuts at: 3367 3367
Size: 4529
ApoI r'AATT_y
Cuts at: 247 346 1533 3882 4138 247
Size: 99 1187 2349 256 638
Fragments arranged by size:
2349 1187 638 256 99
AseI AT'TA_AT
Cuts at: 20 20
Size: 4529
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
AvaI C'yCGr_G
Cuts at: 1549 1549
Size: 4529
BamHI G'GATC_C
Cuts at: 1554 1554
Size: 4529
BanI G'GyrC_C
Cuts at: 487 1042 1545 1959 2207 2242 487
Size: 555 503 414 248 35 2774
Fragments arranged by size:
2774 555 503 414 248 35
BanII G_rGCy'C
Cuts at: 181 1180 1543 2573 181
Size: 999 363 1030 2137
Fragments arranged by size:
2137 1030 999 363
BbeI G_GCGC'C
Cuts at: 1963 2211 1963
Size: 248 4281
BbsI GAAGACnn'nnnn_
Cuts at: 206 206
Size: 4529
BciVI GTATCCnnnnn_n'
Cuts at: 2427 3483 4529 2427
Size: 1056 1046 2427
Fragments arranged by size:
2427 1056 1046
BclI T'GATC_A
Cuts at: 1163 2049 1163
Size: 886 3643
BglI GCCn_nnn'nGGC
Cuts at: 811 811
Size: 4529
BglII A'GATC_T
Cuts at: 2044 4081 2044
Size: 2037 2492
BlpI GC'TnA_GC
Cuts at: 1641 1641
Size: 4529
Bme1580I G_kGCm'C
Cuts at: 490 1180 2154 2247 3371 490
Size: 690 974 93 1124 1648
Fragments arranged by size:
1648 1124 974 690 93
BmgBI CAC'GTC
Cuts at: 1070 1508 1070
Size: 438 4091
BmrI ACTGGGnnnn_n'
Cuts at: 694 1741 1822 2157 4301 694
Size: 1047 81 335 2144 922
Fragments arranged by size:
2144 1047 922 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 523
Size: 4529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 2584 3298 3596 1705
Size: 879 714 298 2638
Fragments arranged by size:
2638 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4233 4233
Size: 4529
BsaAI yAC'GTr
Cuts at: 174 642 1882 174
Size: 468 1240 2821
Fragments arranged by size:
2821 1240 468
BsaBI GATnn'nnATC
Cuts at: 6 2043 4080 6
Size: 2037 2037 455
Fragments arranged by size:
2037 2037 455
BsaHI Gr'CG_yC
Cuts at: 1960 2208 2910 4406 1960
Size: 248 702 1496 2083
Fragments arranged by size:
2083 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1331 1718 2239 2917 3328 3475 3749 1331
Size: 387 521 678 411 147 274 2111
Fragments arranged by size:
2111 678 521 411 387 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 1320 400
Size: 920 3609
BseYI C'CCAG_C
Cuts at: 311 321 445 483 1172 1954 2712 3377 3785
Size: 10 124 38 689 782 758 665 408
Cuts at: 3785 311
Size: 1055
Fragments arranged by size:
1055 782 758 689 665 408 124 38
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 1387
Size: 4529
BsiEI CG_ry'CG
Cuts at: 104 2071 2117 3347 104
Size: 1967 46 1230 1286
Fragments arranged by size:
1967 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1465 1543 2321 2511 3371 1465
Size: 78 778 190 860 2623
Fragments arranged by size:
2623 860 778 190 78
BsmI GAATG_Cn'
Cuts at: 1246 1246
Size: 4529
Bsp1286I G_dGCh'C
Cuts at: 181 490 1180 1465 1543 2154 2247 2321 2511
Size: 309 690 285 78 611 93 74 190
Cuts at: 2511 2573 3371 181
Size: 62 798 1339
Fragments arranged by size:
1339 798 690 611 309 285 190 93
78 74 62
BspEI T'CCGG_A
Cuts at: 1331 1331
Size: 4529
BspHI T'CATG_A
Cuts at: 2961 2961
Size: 4529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1325 1579 1793 2095 2476 1325
Size: 254 214 302 381 3378
Fragments arranged by size:
3378 381 302 254 214
BsrBI CCG'CTC
Cuts at: 50 2821 2875 4359 4524 50
Size: 2771 54 1484 165 55
Fragments arranged by size:
2771 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 461 2441 461
Size: 1980 2549
BsrFI r'CCGG_y
Cuts at: 2527 2708 2527
Size: 181 4348
BssSI C'ACGA_G
Cuts at: 2800 3508 2800
Size: 708 3821
BstAPI GCAn_nnn'nTGC
Cuts at: 1420 1906 1420
Size: 486 4043
BstBI TT'CG_AA
Cuts at: 2890 2890
Size: 4529
BstEII G'GTnAC_C
Cuts at: 951 1092 1482 951
Size: 141 390 3998
Fragments arranged by size:
3998 390 141
BstXI CCAn_nnnn'nTGG
Cuts at: 807 1140 807
Size: 333 4196
BstYI r'GATC_y
Cuts at: 1 1554 2021 2044 2379 2625 2931 2943 3029
Size: 1553 467 23 335 246 306 12 86
Cuts at: 3029 4081 1
Size: 1052 449
Fragments arranged by size:
1553 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 716 806 716
Size: 90 4439
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2479 2660 2823 2479
Size: 181 163 4185
Fragments arranged by size:
4185 181 163
BtsI GCAGTG_nn'
Cuts at: 707 1358 1772 707
Size: 651 414 3464
Fragments arranged by size:
3464 651 414
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 669 704 669
Size: 35 4494
DraI TTT'AAA
Cuts at: 1528 1528
Size: 4529
DrdI GACnn_nn'nnGTC
Cuts at: 2235 3579 2235
Size: 1344 3185
EaeI y'GGCC_r
Cuts at: 458 1099 2114 2288 2679 2706 4051 458
Size: 641 1015 174 391 27 1345 936
Fragments arranged by size:
1345 1015 936 641 391 174 27
EagI C'GGCC_G
Cuts at: 2114 2114
Size: 4529
EarI CTCTTCn'nnn_
Cuts at: 1045 2552 2762 1045
Size: 1507 210 2812
Fragments arranged by size:
2812 1507 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2737 3479 3625 4339 2737
Size: 742 146 714 2927
Fragments arranged by size:
2927 742 714 146
EcoICRI GAG'CTC
Cuts at: 1541 1541
Size: 4529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 2354 2786 3139 3801 523
Size: 1831 432 353 662 1251
Fragments arranged by size:
1831 1251 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 780 780
Size: 4529
EcoO109I rG'GnC_Cy
Cuts at: 767 1176 1177 1314 1668 767
Size: 409 1 137 354 3628
Fragments arranged by size:
3628 409 354 137 1
EcoRI G'AATT_C
Cuts at: 1533 1533
Size: 4529
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 4529
FspI TGC'GCA
Cuts at: 1364 2310 1364
Size: 946 3583
HaeII r_GCGC'y
Cuts at: 1963 2211 3441 4212 1963
Size: 248 1230 771 2280
Fragments arranged by size:
2280 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 2371 2401 2403 2433 205
Size: 32 2134 30 2 30 2301
Fragments arranged by size:
2301 2134 32 30 30 2
HincII GTy'rAC
Cuts at: 1568 1568
Size: 4529
KasI G'GCGC_C
Cuts at: 1959 2207 1959
Size: 248 4281
KpnI G_GTAC'C
Cuts at: 1549 1549
Size: 4529
MluI A'CGCG_T
Cuts at: 3962 3962
Size: 4529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3288 3472 3288
Size: 184 4345
MscI TGG'CCA
Cuts at: 460 2290 460
Size: 1830 2699
MslI CAynn'nnrTG
Cuts at: 920 1492 2518 2645 920
Size: 572 1026 127 2804
Fragments arranged by size:
2804 1026 572 127
MspA1I CmG'CkG
Cuts at: 456 551 1640 1954 2314 3098 3343 456
Size: 95 1089 314 360 784 245 1642
Fragments arranged by size:
1642 1089 784 360 314 245 95
NaeI GCC'GGC
Cuts at: 2710 2710
Size: 4529
NarI GG'CG_CC
Cuts at: 1960 2208 1960
Size: 248 4281
NgoMIV G'CCGG_C
Cuts at: 2708 2708
Size: 4529
NspI r_CATG'y
Cuts at: 993 1341 1582 2082 3685 993
Size: 348 241 500 1603 1837
Fragments arranged by size:
1837 1603 500 348 241
PciI A'CATG_T
Cuts at: 989 3681 989
Size: 2692 1837
PflMI CCAn_nnn'nTGG
Cuts at: 456 456
Size: 4529
PfoI T'CCnGG_A
Cuts at: 500 500
Size: 4529
PmeI GTTT'AAAC
Cuts at: 1528 1528
Size: 4529
PmlI CAC'GTG
Cuts at: 642 642
Size: 4529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 4497
PpuMI rG'GwC_Cy
Cuts at: 1314 1314
Size: 4529
PshAI GACnn'nnGTC
Cuts at: 1141 1141
Size: 4529
PspOMI G'GGCC_C
Cuts at: 1176 1176
Size: 4529
PstI C_TGCA'G
Cuts at: 556 1255 1576 2261 556
Size: 699 321 685 2824
Fragments arranged by size:
2824 699 685 321
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4529
PvuII CAG'CTG
Cuts at: 456 1954 2314 456
Size: 1498 360 2671
Fragments arranged by size:
2671 1498 360
RsrII CG'GwC_CG
Cuts at: 2724 2724
Size: 4529
SacI G_AGCT'C
Cuts at: 1543 1543
Size: 4529
SalI G'TCGA_C
Cuts at: 1566 1566
Size: 4529
SapI GCTCTTCn'nnn_
Cuts at: 1045 2552 2762 1045
Size: 1507 210 2812
Fragments arranged by size:
2812 1507 210
SbfI CC_TGCA'GG
Cuts at: 1576 1576
Size: 4529
ScaI AGT'ACT
Cuts at: 4046 4046
Size: 4529
SexAI A'CCwGG_T
Cuts at: 329 329
Size: 4529
SfcI C'TryA_G
Cuts at: 33 552 621 1251 1572 2257 3225 3416 33
Size: 519 69 630 321 685 968 191 1146
Fragments arranged by size:
1146 968 685 630 519 321 191 69
SfoI GGC'GCC
Cuts at: 1961 2209 1961
Size: 248 4281
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
SmaI CCC'GGG
Cuts at: 1551 1551
Size: 4529
SmlI C'TyrA_G
Cuts at: 1684 2599 3313 3575 1684
Size: 915 714 262 2638
Fragments arranged by size:
2638 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4529
SphI G_CATG'C
Cuts at: 1582 2082 1582
Size: 500 4029
StuI AGG'CCT
Cuts at: 558 558
Size: 4529
StyI C'CwwG_G
Cuts at: 800 1478 1663 2017 800
Size: 678 185 354 3312
Fragments arranged by size:
3312 678 354 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 676 1060 2112 2245 2704 2913 676
Size: 384 1052 133 459 209 2292
Fragments arranged by size:
2292 1052 459 384 209 133
TatI w'GTAC_w
Cuts at: 4044 4044
Size: 4529
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 233 265 409 441 4046 4078 233
Size: 32 144 32 3605 32 684
Fragments arranged by size:
3605 684 144 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2326 2326
Size: 4529
XbaI T'CTAG_A
Cuts at: 1560 1560
Size: 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 807 2024 453
Size: 354 1217 2958
Fragments arranged by size:
2958 1217 354
XmaI C'CCGG_G
Cuts at: 1549 1549
Size: 4529
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI