Restriction map for
pF1KB3072
(Circular) (Six-base) MAPSORT of: pF1KB3072.seq Check: 1629 from: 1 to: 4979
pF1KB3072 4979 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1615 1846 1615
Size: 231 4748
AccI GT'mk_AC
Cuts at: 2017 2017
Size: 4979
Acc65I G'GTAC_C
Cuts at: 1995 1995
Size: 4979
AclI AA'CG_TT
Cuts at: 4819 4819
Size: 4979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1066 2804 3236 3983 4251 736
Size: 330 1738 432 747 268 1464
Fragments arranged by size:
1738 1464 747 432 330 268
AflIII A'CryG_T
Cuts at: 2331 3435 4412 2331
Size: 1104 977 2898
Fragments arranged by size:
2898 1104 977
AgeI A'CCGG_T
Cuts at: 1904 3367 1904
Size: 1463 3516
AhdI GACnn_n'nnGTC
Cuts at: 1104 1667 1104
Size: 563 4416
AleI CACnn'nnGTG
Cuts at: 163 163
Size: 4979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1078 1645 1078
Size: 567 4412
AlwNI CAG_nnn'CTG
Cuts at: 232 473 564 606 720 903 984 1188 1560
Size: 241 91 42 114 183 81 204 372
Cuts at: 1560 1779 2284 3851 232
Size: 219 505 1567 1360
Fragments arranged by size:
1567 1360 505 372 241 219 204 183
114 91 81 42
ApaLI G'TGCA_C
Cuts at: 1297 3749 1297
Size: 2452 2527
ApoI r'AATT_y
Cuts at: 309 426 1983 4332 4588 309
Size: 117 1557 2349 256 700
Fragments arranged by size:
2349 1557 700 256 117
AseI AT'TA_AT
Cuts at: 20 20
Size: 4979
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
AvaI C'yCGr_G
Cuts at: 263 1012 1116 1354 1748 1999 263
Size: 749 104 238 394 251 3243
Fragments arranged by size:
3243 749 394 251 238 104
BamHI G'GATC_C
Cuts at: 2004 2004
Size: 4979
BanI G'GyrC_C
Cuts at: 157 441 1995 2409 2657 2692 157
Size: 284 1554 414 248 35 2444
Fragments arranged by size:
2444 1554 414 284 248 35
BanII G_rGCy'C
Cuts at: 124 226 322 557 974 1729 1993 3023 124
Size: 102 96 235 417 755 264 1030 2080
Fragments arranged by size:
2080 1030 755 417 264 235 102 96
BbeI G_GCGC'C
Cuts at: 2413 2661 2413
Size: 248 4731
BbvCI CC'TCA_GC
Cuts at: 245 245
Size: 4979
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1593 1627 1593
Size: 34 4945
BciVI GTATCCnnnnn_n'
Cuts at: 899 1580 2877 3638 4979 899
Size: 681 1297 761 1341 899
Fragments arranged by size:
1341 1297 899 761 681
BclI T'GATC_A
Cuts at: 2499 2499
Size: 4979
BglI GCCn_nnn'nGGC
Cuts at: 139 1085 139
Size: 946 4033
BglII A'GATC_T
Cuts at: 2494 4531 2494
Size: 2037 2942
BlpI GC'TnA_GC
Cuts at: 225 1321 1546 2091 225
Size: 1096 225 545 3113
Fragments arranged by size:
3113 1096 545 225
Bme1580I G_kGCm'C
Cuts at: 1067 1301 1867 2604 2697 3753 1067
Size: 234 566 737 93 1056 2293
Fragments arranged by size:
2293 1056 737 566 234 93
BmrI ACTGGGnnnn_n'
Cuts at: 916 2191 2272 2607 4751 916
Size: 1275 81 335 2144 1144
Fragments arranged by size:
2144 1275 1144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 408 1142 3346 408
Size: 734 2204 2041
Fragments arranged by size:
2204 2041 734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 281 323 541 1014 1303 1327 1446 1582
Size: 9 42 218 473 289 24 119 136
Cuts at: 1582 272
Size: 3669
Fragments arranged by size:
3669 473 289 218 136 119 42 24
9
Bpu10I CC'TnA_GC
Cuts at: 245 245
Size: 4979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2155 3034 3526 3824 2155
Size: 879 492 298 3310
Fragments arranged by size:
3310 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 248 363 4683 248
Size: 115 4320 544
Fragments arranged by size:
4320 544 115
BsaAI yAC'GTr
Cuts at: 1692 2332 1692
Size: 640 4339
BsaBI GATnn'nnATC
Cuts at: 6 2493 4530 6
Size: 2487 2037 455
Fragments arranged by size:
2487 2037 455
BsaHI Gr'CG_yC
Cuts at: 1668 2410 2658 3360 4856 1668
Size: 742 248 702 1496 1791
Fragments arranged by size:
1791 1496 742 702 248
BsaWI w'CCGG_w
Cuts at: 1904 2168 2689 3367 3641 3788 4199 1904
Size: 264 521 678 274 147 411 2684
Fragments arranged by size:
2684 678 521 411 274 264 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 132 202 1719 1867 132
Size: 70 1517 148 3244
Fragments arranged by size:
3244 1517 148 70
BseYI C'CCAG_C
Cuts at: 134 258 795 842 977 1075 1266 2404 3162
Size: 124 537 47 135 98 191 1138 758
Cuts at: 3162 3739 4235 134
Size: 577 496 878
Fragments arranged by size:
1138 878 758 577 537 496 191 135
124 98 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 889 1174 1738 156
Size: 733 285 564 3397
Fragments arranged by size:
3397 733 564 285
BsiEI CG_ry'CG
Cuts at: 104 2521 2567 3775 104
Size: 2417 46 1208 1308
Fragments arranged by size:
2417 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 557 1301 1729 1790 1967 1993 2771 2961 3753
Size: 744 428 61 177 26 778 190 792
Cuts at: 3753 557
Size: 1783
Fragments arranged by size:
1783 792 778 744 428 190 177 61
26
Bsp1286I G_dGCh'C
Cuts at: 124 226 322 557 974 1067 1301 1729 1790
Size: 102 96 235 417 93 234 428 61
Cuts at: 1790 1867 1967 1993 2604 2697 2771 2961 3023
Size: 77 100 26 611 93 74 190 62
Cuts at: 3023 3753 124
Size: 730 1350
Fragments arranged by size:
1350 730 611 428 417 235 234 190
102 100 96 93 93 77 74 62
61 26
BspEI T'CCGG_A
Cuts at: 4199 4199
Size: 4979
BspHI T'CATG_A
Cuts at: 569 788 4155 569
Size: 219 3367 1393
Fragments arranged by size:
3367 1393 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 759 1109 1615 1846 2029 2243 2545 2926 759
Size: 350 506 231 183 214 302 381 2812
Fragments arranged by size:
2812 506 381 350 302 231 214 183
BsrBI CCG'CTC
Cuts at: 50 1278 1422 3271 3325 4809 4974 50
Size: 1228 144 1849 54 1484 165 55
Fragments arranged by size:
1849 1484 1228 165 144 55 54
BsrDI GCAATG_nn'
Cuts at: 2891 2891
Size: 4979
BsrFI r'CCGG_y
Cuts at: 1550 1904 2977 3158 3367 1550
Size: 354 1073 181 209 3162
Fragments arranged by size:
3162 1073 354 209 181
BssSI C'ACGA_G
Cuts at: 208 3250 3608 208
Size: 3042 358 1579
Fragments arranged by size:
3042 1579 358
BstAPI GCAn_nnn'nTGC
Cuts at: 903 1188 2356 903
Size: 285 1168 3526
Fragments arranged by size:
3526 1168 285
BstBI TT'CG_AA
Cuts at: 3340 3340
Size: 4979
BstYI r'GATC_y
Cuts at: 1 761 1430 1475 1483 2004 2471 2494 2829
Size: 760 669 45 8 521 467 23 335
Cuts at: 2829 3075 4087 4173 4185 4531 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 760 669 521 467 449 346 335
246 86 45 23 12 8
BtgI C'CryG_G
Cuts at: 161 944 1080 1591 161
Size: 783 136 511 3549
Fragments arranged by size:
3549 783 511 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1961 2929 3110 3273 1961
Size: 968 181 163 3667
Fragments arranged by size:
3667 968 181 163
BtsI GCAGTG_nn'
Cuts at: 230 2222 230
Size: 1992 2987
DraI TTT'AAA
Cuts at: 1978 1978
Size: 4979
DraIII CAC_nnn'GTG
Cuts at: 1884 1884
Size: 4979
DrdI GACnn_nn'nnGTC
Cuts at: 2685 3543 2685
Size: 858 4121
EaeI y'GGCC_r
Cuts at: 947 1710 2564 2738 3129 3156 4501 947
Size: 763 854 174 391 27 1345 1425
Fragments arranged by size:
1425 1345 854 763 391 174 27
EagI C'GGCC_G
Cuts at: 2564 2564
Size: 4979
EarI CTCTTCn'nnn_
Cuts at: 925 1931 1936 3002 3212 925
Size: 1006 5 1066 210 2692
Fragments arranged by size:
2692 1066 1006 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3187 3497 3643 4789 3187
Size: 310 146 1146 3377
Fragments arranged by size:
3377 1146 310 146
EcoICRI GAG'CTC
Cuts at: 555 1727 1991 555
Size: 1172 264 3543
Fragments arranged by size:
3543 1172 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 281 323 541 736 1014 1066 1303 1327
Size: 9 42 218 195 278 52 237 24
Cuts at: 1327 1446 1582 2804 3236 3983 4251 272
Size: 119 136 1222 432 747 268 1000
Fragments arranged by size:
1222 1000 747 432 278 268 237 218
195 136 119 52 42 24 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1849 1849
Size: 4979
EcoO109I rG'GnC_Cy
Cuts at: 213 353 527 1008 1087 1098 1205 1242 1650
Size: 140 174 481 79 11 107 37 408
Cuts at: 1650 1661 2118 213
Size: 11 457 3074
Fragments arranged by size:
3074 481 457 408 174 140 107 79
37 11 11
EcoRI G'AATT_C
Cuts at: 1983 1983
Size: 4979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 432 2998 432
Size: 2566 2413
FspI TGC'GCA
Cuts at: 2760 2760
Size: 4979
HaeII r_GCGC'y
Cuts at: 2413 2661 3683 4662 2413
Size: 248 1022 979 2730
Fragments arranged by size:
2730 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 319 351 1090 1122 1653 1685 1864 1896 1909
Size: 32 739 32 531 32 179 32 13
Cuts at: 1909 1941 2821 2851 2853 2883 319
Size: 32 880 30 2 30 2415
Fragments arranged by size:
2415 880 739 531 179 32 32 32
32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 774 1833 2018 774
Size: 1059 185 3735
Fragments arranged by size:
3735 1059 185
HpaI GTT'AAC
Cuts at: 774 774
Size: 4979
KasI G'GCGC_C
Cuts at: 2409 2657 2409
Size: 248 4731
KpnI G_GTAC'C
Cuts at: 1999 1999
Size: 4979
MluI A'CGCG_T
Cuts at: 4412 4412
Size: 4979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 362 3650 3834 362
Size: 3288 184 1507
Fragments arranged by size:
3288 1507 184
MscI TGG'CCA
Cuts at: 949 1712 2740 949
Size: 763 1028 3188
Fragments arranged by size:
3188 1028 763
MslI CAynn'nnrTG
Cuts at: 112 163 1822 2968 3095 112
Size: 51 1659 1146 127 1996
Fragments arranged by size:
1996 1659 1146 127 51
MspA1I CmG'CkG
Cuts at: 138 238 582 720 846 894 900 1058 1185
Size: 100 344 138 126 48 6 158 127
Cuts at: 1185 1593 1767 2090 2404 2764 3777 4022 138
Size: 408 174 323 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 408 360 344 323 314 245
174 158 138 127 126 100 48 6
NaeI GCC'GGC
Cuts at: 1552 3160 1552
Size: 1608 3371
NarI GG'CG_CC
Cuts at: 2410 2658 2410
Size: 248 4731
NcoI C'CATG_G
Cuts at: 161 944 1080 161
Size: 783 136 4060
Fragments arranged by size:
4060 783 136
NgoMIV G'CCGG_C
Cuts at: 1550 3158 1550
Size: 1608 3371
NspI r_CATG'y
Cuts at: 780 1455 1764 1821 2032 2532 3439 780
Size: 675 309 57 211 500 907 2320
Fragments arranged by size:
2320 907 675 500 309 211 57
PasI CC'CwG_GG
Cuts at: 219 303 219
Size: 84 4895
PciI A'CATG_T
Cuts at: 3435 3435
Size: 4979
PflMI CCAn_nnn'nTGG
Cuts at: 473 564 473
Size: 91 4888
PfoI T'CCnGG_A
Cuts at: 1169 1169
Size: 4979
PmeI GTTT'AAAC
Cuts at: 1978 1978
Size: 4979
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4947
PpuMI rG'GwC_Cy
Cuts at: 353 527 1098 1242 1661 353
Size: 174 571 144 419 3671
Fragments arranged by size:
3671 571 419 174 144
PstI C_TGCA'G
Cuts at: 899 968 1106 1843 2026 2711 899
Size: 69 138 737 183 685 3167
Fragments arranged by size:
3167 737 685 183 138 69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4979
PvuII CAG'CTG
Cuts at: 138 582 720 846 900 1058 1185 2404 2764
Size: 444 138 126 54 158 127 1219 360
Cuts at: 2764 138
Size: 2353
Fragments arranged by size:
2353 1219 444 360 158 138 127 126
54
RsrII CG'GwC_CG
Cuts at: 3174 3174
Size: 4979
SacI G_AGCT'C
Cuts at: 557 1729 1993 557
Size: 1172 264 3543
Fragments arranged by size:
3543 1172 264
SacII CC_GC'GG
Cuts at: 1594 1594
Size: 4979
SalI G'TCGA_C
Cuts at: 2016 2016
Size: 4979
SanDI GG'GwC_CC
Cuts at: 353 527 353
Size: 174 4805
SapI GCTCTTCn'nnn_
Cuts at: 1936 3002 3212 1936
Size: 1066 210 3703
Fragments arranged by size:
3703 1066 210
SbfI CC_TGCA'GG
Cuts at: 968 2026 968
Size: 1058 3921
ScaI AGT'ACT
Cuts at: 4496 4496
Size: 4979
SfcI C'TryA_G
Cuts at: 33 330 895 964 1102 1839 2022 2707 3700
Size: 297 565 69 138 737 183 685 993
Cuts at: 3700 3891 33
Size: 191 1121
Fragments arranged by size:
1121 993 737 685 565 297 191 183
138 69
SfoI GGC'GCC
Cuts at: 2411 2659 2411
Size: 248 4731
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
SmaI CCC'GGG
Cuts at: 265 2001 265
Size: 1736 3243
SmlI C'TyrA_G
Cuts at: 2134 3049 3541 3803 2134
Size: 915 492 262 3310
Fragments arranged by size:
3310 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4979
SphI G_CATG'C
Cuts at: 1764 2032 2532 1764
Size: 268 500 4211
Fragments arranged by size:
4211 500 268
SrfI GCCC'GGGC
Cuts at: 265 265
Size: 4979
StuI AGG'CCT
Cuts at: 936 936
Size: 4979
StyI C'CwwG_G
Cuts at: 161 944 1004 1080 1137 1208 1271 1412 1479
Size: 783 60 76 57 71 63 141 67
Cuts at: 1479 2113 2467 161
Size: 634 354 2673
Fragments arranged by size:
2673 783 634 354 141 76 71 67
63 60 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 967 1316 2562 2695 3154 3363 967
Size: 349 1246 133 459 209 2583
Fragments arranged by size:
2583 1246 459 349 209 133
TatI w'GTAC_w
Cuts at: 1583 1643 4494 1583
Size: 60 2851 2068
Fragments arranged by size:
2851 2068 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 568 600 4496 4528 568
Size: 32 3896 32 1019
Fragments arranged by size:
3896 1019 32 32
Tth111I GACn'n_nGTC
Cuts at: 1153 2776 1153
Size: 1623 3356
XbaI T'CTAG_A
Cuts at: 2010 2010
Size: 4979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1278 2474 1278
Size: 1196 3783
XmaI C'CCGG_G
Cuts at: 263 1999 263
Size: 1736 3243
XmnI GAAnn'nnTTC
Cuts at: 1396 1396
Size: 4979
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI ApaI AscI AvrII BaeI
BbsI BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PspXI
PsrI SexAI SfiI SgrAI SnaBI SspI SwaI XhoI
ZraI