Restriction map for
pF1KB3077
(Circular) (Six-base) MAPSORT of: pF1KB3077.seq Check: 3383 from: 1 to: 3899
pF1KB3077 3899 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 872 937 872
Size: 65 3834
Acc65I G'GTAC_C
Cuts at: 915 915
Size: 3899
AclI AA'CG_TT
Cuts at: 3739 3739
Size: 3899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1724 2156 2903 3171 1724
Size: 432 747 268 2452
Fragments arranged by size:
2452 747 432 268
AflIII A'CryG_T
Cuts at: 638 1251 2355 3332 638
Size: 613 1104 977 1205
Fragments arranged by size:
1205 1104 977 613
AgeI A'CCGG_T
Cuts at: 2287 2287
Size: 3899
AlwNI CAG_nnn'CTG
Cuts at: 1204 2771 1204
Size: 1567 2332
ApaLI G'TGCA_C
Cuts at: 581 2669 581
Size: 2088 1811
ApoI r'AATT_y
Cuts at: 903 3252 3508 903
Size: 2349 256 1294
Fragments arranged by size:
2349 1294 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3899
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
AvaI C'yCGr_G
Cuts at: 919 919
Size: 3899
BamHI G'GATC_C
Cuts at: 924 924
Size: 3899
BanI G'GyrC_C
Cuts at: 345 915 1329 1577 1612 345
Size: 570 414 248 35 2632
Fragments arranged by size:
2632 570 414 248 35
BanII G_rGCy'C
Cuts at: 913 1943 913
Size: 1030 2869
BbeI G_GCGC'C
Cuts at: 1333 1581 1333
Size: 248 3651
BbsI GAAGACnn'nnnn_
Cuts at: 118 118
Size: 3899
BciVI GTATCCnnnnn_n'
Cuts at: 1797 2558 3899 1797
Size: 761 1341 1797
Fragments arranged by size:
1797 1341 761
BclI T'GATC_A
Cuts at: 1419 1419
Size: 3899
BglI GCCn_nnn'nGGC
Cuts at: 749 749
Size: 3899
BglII A'GATC_T
Cuts at: 1414 3451 1414
Size: 2037 1862
BlpI GC'TnA_GC
Cuts at: 1011 1011
Size: 3899
Bme1580I G_kGCm'C
Cuts at: 585 1524 1617 2673 585
Size: 939 93 1056 1811
Fragments arranged by size:
1811 1056 939 93
BmrI ACTGGGnnnn_n'
Cuts at: 312 1111 1192 1527 3671 312
Size: 799 81 335 2144 540
Fragments arranged by size:
2144 799 540 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 3899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 692 188
Size: 504 3395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1954 2446 2744 1075
Size: 879 492 298 2230
Fragments arranged by size:
2230 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3603 3603
Size: 3899
BsaAI yAC'GTr
Cuts at: 580 1252 580
Size: 672 3227
BsaBI GATnn'nnATC
Cuts at: 6 1413 3450 6
Size: 1407 2037 455
Fragments arranged by size:
2037 1407 455
BsaHI Gr'CG_yC
Cuts at: 1330 1578 2280 3776 1330
Size: 248 702 1496 1453
Fragments arranged by size:
1496 1453 702 248
BsaWI w'CCGG_w
Cuts at: 1088 1609 2287 2561 2708 3119 1088
Size: 521 678 274 147 411 1868
Fragments arranged by size:
1868 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 719 749 719
Size: 30 3869
BseYI C'CCAG_C
Cuts at: 248 1324 2082 2659 3155 248
Size: 1076 758 577 496 992
Fragments arranged by size:
1076 992 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1441 1487 2695 104
Size: 1337 46 1208 1308
Fragments arranged by size:
1337 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 585 913 1691 1881 2673 585
Size: 328 778 190 792 1811
Fragments arranged by size:
1811 792 778 328 190
Bsp1286I G_dGCh'C
Cuts at: 585 913 1524 1617 1691 1881 1943 2673 585
Size: 328 611 93 74 190 62 730 1811
Fragments arranged by size:
1811 730 611 328 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3119 3119
Size: 3899
BspHI T'CATG_A
Cuts at: 590 3075 590
Size: 2485 1414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 949 1163 1465 1846 949
Size: 214 302 381 3002
Fragments arranged by size:
3002 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2191 2245 3729 3894 50
Size: 2141 54 1484 165 55
Fragments arranged by size:
2141 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1811 1811
Size: 3899
BsrFI r'CCGG_y
Cuts at: 1897 2078 2287 1897
Size: 181 209 3509
Fragments arranged by size:
3509 209 181
BsrGI T'GTAC_A
Cuts at: 635 635
Size: 3899
BssSI C'ACGA_G
Cuts at: 2170 2528 2170
Size: 358 3541
BstAPI GCAn_nnn'nTGC
Cuts at: 163 1276 163
Size: 1113 2786
BstBI TT'CG_AA
Cuts at: 2260 2260
Size: 3899
BstYI r'GATC_y
Cuts at: 1 924 1391 1414 1749 1995 3007 3093 3105
Size: 923 467 23 335 246 1012 86 12
Cuts at: 3105 3451 1
Size: 346 449
Fragments arranged by size:
1012 923 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 494 1849 2030 2193 494
Size: 1355 181 163 2200
Fragments arranged by size:
2200 1355 181 163
BtsI GCAGTG_nn'
Cuts at: 228 521 1142 228
Size: 293 621 2985
Fragments arranged by size:
2985 621 293
DraI TTT'AAA
Cuts at: 898 898
Size: 3899
DrdI GACnn_nn'nnGTC
Cuts at: 1605 2463 1605
Size: 858 3041
EaeI y'GGCC_r
Cuts at: 135 1484 1658 2049 2076 3421 135
Size: 1349 174 391 27 1345 613
Fragments arranged by size:
1349 1345 613 391 174 27
EagI C'GGCC_G
Cuts at: 1484 1484
Size: 3899
EarI CTCTTCn'nnn_
Cuts at: 187 1922 2132 187
Size: 1735 210 1954
Fragments arranged by size:
1954 1735 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 273 2107 2417 2563 3709 273
Size: 1834 310 146 1146 463
Fragments arranged by size:
1834 1146 463 310 146
EcoICRI GAG'CTC
Cuts at: 911 911
Size: 3899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 692 1724 2156 2903 3171 188
Size: 504 1032 432 747 268 916
Fragments arranged by size:
1032 916 747 504 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1038 1038
Size: 3899
EcoRI G'AATT_C
Cuts at: 903 903
Size: 3899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 691 1918 691
Size: 1227 2672
FspI TGC'GCA
Cuts at: 1680 1680
Size: 3899
HaeII r_GCGC'y
Cuts at: 1333 1581 2603 3582 1333
Size: 248 1022 979 1650
Fragments arranged by size:
1650 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1741 1771 1773 1803 1741
Size: 30 2 30 3837
Fragments arranged by size:
3837 30 30 2
HincII GTy'rAC
Cuts at: 938 938
Size: 3899
KasI G'GCGC_C
Cuts at: 1329 1577 1329
Size: 248 3651
KpnI G_GTAC'C
Cuts at: 919 919
Size: 3899
MluI A'CGCG_T
Cuts at: 3332 3332
Size: 3899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2570 2754 2570
Size: 184 3715
MscI TGG'CCA
Cuts at: 137 1660 137
Size: 1523 2376
MslI CAynn'nnrTG
Cuts at: 866 1888 2015 866
Size: 1022 127 2750
Fragments arranged by size:
2750 1022 127
MspA1I CmG'CkG
Cuts at: 141 1010 1324 1684 2697 2942 141
Size: 869 314 360 1013 245 1098
Fragments arranged by size:
1098 1013 869 360 314 245
NaeI GCC'GGC
Cuts at: 2080 2080
Size: 3899
NarI GG'CG_CC
Cuts at: 1330 1578 1330
Size: 248 3651
NdeI CA'TA_TG
Cuts at: 524 524
Size: 3899
NgoMIV G'CCGG_C
Cuts at: 2078 2078
Size: 3899
NspI r_CATG'y
Cuts at: 642 952 1452 2359 642
Size: 310 500 907 2182
Fragments arranged by size:
2182 907 500 310
PciI A'CATG_T
Cuts at: 638 2355 638
Size: 1717 2182
PmeI GTTT'AAAC
Cuts at: 898 898
Size: 3899
PmlI CAC'GTG
Cuts at: 580 580
Size: 3899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 3867
PsiI TTA'TAA
Cuts at: 373 373
Size: 3899
PstI C_TGCA'G
Cuts at: 277 946 1631 277
Size: 669 685 2545
Fragments arranged by size:
2545 685 669
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3899
PvuII CAG'CTG
Cuts at: 1324 1684 1324
Size: 360 3539
RsrII CG'GwC_CG
Cuts at: 2094 2094
Size: 3899
SacI G_AGCT'C
Cuts at: 913 913
Size: 3899
SalI G'TCGA_C
Cuts at: 936 936
Size: 3899
SapI GCTCTTCn'nnn_
Cuts at: 1922 2132 1922
Size: 210 3689
SbfI CC_TGCA'GG
Cuts at: 946 946
Size: 3899
ScaI AGT'ACT
Cuts at: 3416 3416
Size: 3899
SexAI A'CCwGG_T
Cuts at: 341 341
Size: 3899
SfcI C'TryA_G
Cuts at: 33 273 942 1627 2620 2811 33
Size: 240 669 685 993 191 1121
Fragments arranged by size:
1121 993 685 669 240 191
SfoI GGC'GCC
Cuts at: 1331 1579 1331
Size: 248 3651
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
SmaI CCC'GGG
Cuts at: 921 921
Size: 3899
SmlI C'TyrA_G
Cuts at: 1054 1969 2461 2723 1054
Size: 915 492 262 2230
Fragments arranged by size:
2230 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3899
SphI G_CATG'C
Cuts at: 952 1452 952
Size: 500 3399
StyI C'CwwG_G
Cuts at: 1033 1387 1033
Size: 354 3545
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 278 1482 1615 2074 2283 278
Size: 1204 133 459 209 1894
Fragments arranged by size:
1894 1204 459 209 133
TatI w'GTAC_w
Cuts at: 635 3414 635
Size: 2779 1120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 719 751 3416 3448 719
Size: 32 2665 32 1170
Fragments arranged by size:
2665 1170 32 32
Tth111I GACn'n_nGTC
Cuts at: 628 1696 628
Size: 1068 2831
XbaI T'CTAG_A
Cuts at: 930 930
Size: 3899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1394 1394
Size: 3899
XmaI C'CCGG_G
Cuts at: 919 919
Size: 3899
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PciI PmeI PmlI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI