Restriction map for
pF1KB3078
(Circular) (Six-base) MAPSORT of: pF1KB3078.seq Check: 958 from: 1 to: 5936
pF1KB3078 5936 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 972 972
Size: 5936
AccI GT'mk_AC
Cuts at: 2195 2974 2195
Size: 779 5157
Acc65I G'GTAC_C
Cuts at: 2952 2952
Size: 5936
AclI AA'CG_TT
Cuts at: 5776 5776
Size: 5936
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 856 3761 4193 4546 5208 688
Size: 168 2905 432 353 662 1416
Fragments arranged by size:
2905 1416 662 432 353 168
AflIII A'CryG_T
Cuts at: 920 2892 3288 5088 5369 920
Size: 1972 396 1800 281 1487
Fragments arranged by size:
1972 1800 1487 396 281
AleI CACnn'nnGTG
Cuts at: 2443 2443
Size: 5936
AlwNI CAG_nnn'CTG
Cuts at: 1778 1896 2858 3241 4679 1778
Size: 118 962 383 1438 3035
Fragments arranged by size:
3035 1438 962 383 118
ApaI G_GGCC'C
Cuts at: 1160 1160
Size: 5936
ApaLI G'TGCA_C
Cuts at: 985 4774 985
Size: 3789 2147
ApoI r'AATT_y
Cuts at: 1387 2940 5289 5545 1387
Size: 1553 2349 256 1778
Fragments arranged by size:
2349 1778 1553 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5936
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5936
AvaI C'yCGr_G
Cuts at: 136 217 1579 2246 2922 2956 136
Size: 81 1362 667 676 34 3116
Fragments arranged by size:
3116 1362 676 667 81 34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1920 1953 1920
Size: 33 5903
BamHI G'GATC_C
Cuts at: 1259 1486 1729 2961 1259
Size: 227 243 1232 4234
Fragments arranged by size:
4234 1232 243 227
BanI G'GyrC_C
Cuts at: 213 241 1250 2952 3366 3614 3649 213
Size: 28 1009 1702 414 248 35 2500
Fragments arranged by size:
2500 1702 1009 414 248 35 28
BanII G_rGCy'C
Cuts at: 127 1070 1078 1160 2609 2720 2950 3980 127
Size: 943 8 82 1449 111 230 1030 2083
Fragments arranged by size:
2083 1449 1030 943 230 111 82 8
BbeI G_GCGC'C
Cuts at: 217 245 3370 3618 217
Size: 28 3125 248 2535
Fragments arranged by size:
3125 2535 248 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2163 2197 2163
Size: 34 5902
BciVI GTATCCnnnnn_n'
Cuts at: 1948 3834 4890 5936 1948
Size: 1886 1056 1046 1948
Fragments arranged by size:
1948 1886 1056 1046
BclI T'GATC_A
Cuts at: 1586 3456 1586
Size: 1870 4066
BglI GCCn_nnn'nGGC
Cuts at: 2474 2474
Size: 5936
BglII A'GATC_T
Cuts at: 1535 3451 5488 1535
Size: 1916 2037 1983
Fragments arranged by size:
2037 1983 1916
BlpI GC'TnA_GC
Cuts at: 3048 3048
Size: 5936
Bme1580I G_kGCm'C
Cuts at: 706 989 1160 3561 3654 4778 706
Size: 283 171 2401 93 1124 1864
Fragments arranged by size:
2401 1864 1124 283 171 93
BmrI ACTGGGnnnn_n'
Cuts at: 3148 3229 3564 5708 3148
Size: 81 335 2144 3376
Fragments arranged by size:
3376 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 819 1708 4303 819
Size: 889 2595 2452
Fragments arranged by size:
2595 2452 889
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 1474 1540 1854 2062 2491 2744 2773 776
Size: 698 66 314 208 429 253 29 3939
Fragments arranged by size:
3939 698 429 314 253 208 66 29
Bpu10I CC'TnA_GC
Cuts at: 1072 1072
Size: 5936
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 3112 3991 4705 5003 661
Size: 2451 879 714 298 1594
Fragments arranged by size:
2451 1594 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1315 2131 2925 5640 1315
Size: 816 794 2715 1611
Fragments arranged by size:
2715 1611 816 794
BsaAI yAC'GTr
Cuts at: 3289 3289
Size: 5936
BsaBI GATnn'nnATC
Cuts at: 6 2687 3450 5487 6
Size: 2681 763 2037 455
Fragments arranged by size:
2681 2037 763 455
BsaHI Gr'CG_yC
Cuts at: 214 242 506 2840 3367 3615 4317 5813 214
Size: 28 264 2334 527 248 702 1496 337
Fragments arranged by size:
2334 1496 702 527 337 264 248 28
BsaWI w'CCGG_w
Cuts at: 1016 1358 1625 3125 3646 4324 4735 4882 5156
Size: 342 267 1500 521 678 411 147 274
Cuts at: 5156 1016
Size: 1796
Fragments arranged by size:
1796 1500 678 521 411 342 274 267
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 907 937 907
Size: 30 5906
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 928 928
Size: 5936
BseYI C'CCAG_C
Cuts at: 230 259 1159 1290 2007 2693 3361 4119 4784
Size: 29 900 131 717 686 668 758 665
Cuts at: 4784 5192 230
Size: 408 974
Fragments arranged by size:
974 900 758 717 686 668 665 408
131 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 2284 583
Size: 1701 4235
BsiEI CG_ry'CG
Cuts at: 104 253 1805 3478 3524 4754 104
Size: 149 1552 1673 46 1230 1286
Fragments arranged by size:
1673 1552 1286 1230 149 46
BsiHKAI G_wGCw'C
Cuts at: 127 341 989 1078 2048 2609 2647 2950 3728
Size: 214 648 89 970 561 38 303 778
Cuts at: 3728 3918 4778 127
Size: 190 860 1285
Fragments arranged by size:
1285 970 860 778 648 561 303 214
190 89 38
Bsp1286I G_dGCh'C
Cuts at: 127 341 706 989 1070 1078 1160 2048 2609
Size: 214 365 283 81 8 82 888 561
Cuts at: 2609 2647 2720 2950 3561 3654 3728 3918 3980
Size: 38 73 230 611 93 74 190 62
Cuts at: 3980 4778 127
Size: 798 1285
Fragments arranged by size:
1285 888 798 611 561 365 283 230
214 190 93 82 81 74 73 62
38 8
BspEI T'CCGG_A
Cuts at: 1016 1358 1016
Size: 342 5594
BspHI T'CATG_A
Cuts at: 4368 4368
Size: 5936
BspMI ACCTGCnnnn'nnnn_
Cuts at: 416 972 2986 3200 3502 3883 416
Size: 556 2014 214 302 381 2469
Fragments arranged by size:
2469 2014 556 381 302 214
BsrBI CCG'CTC
Cuts at: 50 273 297 4228 4282 5766 5931 50
Size: 223 24 3931 54 1484 165 55
Fragments arranged by size:
3931 1484 223 165 55 54 24
BsrDI GCAATG_nn'
Cuts at: 530 3848 530
Size: 3318 2618
BsrFI r'CCGG_y
Cuts at: 1247 3934 4115 1247
Size: 2687 181 3068
Fragments arranged by size:
3068 2687 181
BssHII G'CGCG_C
Cuts at: 169 169
Size: 5936
BssSI C'ACGA_G
Cuts at: 4207 4915 4207
Size: 708 5228
BstAPI GCAn_nnn'nTGC
Cuts at: 3313 3313
Size: 5936
BstBI TT'CG_AA
Cuts at: 4297 4297
Size: 5936
BstEII G'GTnAC_C
Cuts at: 2436 2541 2436
Size: 105 5831
BstXI CCAn_nnnn'nTGG
Cuts at: 309 1518 309
Size: 1209 4727
BstYI r'GATC_y
Cuts at: 1 368 1259 1486 1535 1729 2736 2961 3428
Size: 367 891 227 49 194 1007 225 467
Cuts at: 3428 3451 3786 4032 4338 4350 4436 5488 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 1007 891 467 449 367 335 306
246 227 225 194 86 49 23 12
BtgI C'CryG_G
Cuts at: 107 118 347 1037 1170 1223 1790 107
Size: 11 229 690 133 53 567 4253
Fragments arranged by size:
4253 690 567 229 133 53 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1185 2479 3886 4067 4230 1185
Size: 1294 1407 181 163 2891
Fragments arranged by size:
2891 1407 1294 181 163
BtsI GCAGTG_nn'
Cuts at: 1210 1231 3179 1210
Size: 21 1948 3967
Fragments arranged by size:
3967 1948 21
DraI TTT'AAA
Cuts at: 2669 2935 2669
Size: 266 5670
DraIII CAC_nnn'GTG
Cuts at: 428 428
Size: 5936
DrdI GACnn_nn'nnGTC
Cuts at: 3642 4986 3642
Size: 1344 4592
EaeI y'GGCC_r
Cuts at: 250 311 356 864 1442 1592 3521 3695 4086
Size: 61 45 508 578 150 1929 174 391
Cuts at: 4086 4113 5458 250
Size: 27 1345 728
Fragments arranged by size:
1929 1345 728 578 508 391 174 150
61 45 27
EagI C'GGCC_G
Cuts at: 250 3521 250
Size: 3271 2665
EarI CTCTTCn'nnn_
Cuts at: 1612 2531 3959 4169 1612
Size: 919 1428 210 3379
Fragments arranged by size:
3379 1428 919 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2020 4144 4886 5032 5746 2020
Size: 2124 742 146 714 2210
Fragments arranged by size:
2210 2124 742 714 146
EcoICRI GAG'CTC
Cuts at: 125 1076 2607 2948 125
Size: 951 1531 341 3113
Fragments arranged by size:
3113 1531 951 341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 776 856 1474 1540 1854 2062 2491 2744
Size: 88 80 618 66 314 208 429 253
Cuts at: 2744 2773 3761 4193 4546 5208 688
Size: 29 988 432 353 662 1416
Fragments arranged by size:
1416 988 662 618 432 429 353 314
253 208 88 80 66 29
EcoO109I rG'GnC_Cy
Cuts at: 221 1156 1775 2475 3075 221
Size: 935 619 700 600 3082
Fragments arranged by size:
3082 935 700 619 600
EcoRI G'AATT_C
Cuts at: 2940 2940
Size: 5936
EcoRV GAT'ATC
Cuts at: 2022 2022
Size: 5936
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3955 3955
Size: 5936
FspI TGC'GCA
Cuts at: 3717 3717
Size: 5936
HaeII r_GCGC'y
Cuts at: 217 245 3370 3618 4848 5619 217
Size: 28 3125 248 1230 771 534
Fragments arranged by size:
3125 1230 771 534 248 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1638 1670 1721 1753 2006 2038 2740 2772 2903
Size: 32 51 32 253 32 702 32 131
Cuts at: 2903 2935 3778 3808 3810 3840 1638
Size: 32 843 30 2 30 3734
Fragments arranged by size:
3734 843 702 253 131 51 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2975 2975
Size: 5936
KasI G'GCGC_C
Cuts at: 213 241 3366 3614 213
Size: 28 3125 248 2535
Fragments arranged by size:
3125 2535 248 28
KpnI G_GTAC'C
Cuts at: 2956 2956
Size: 5936
MluI A'CGCG_T
Cuts at: 5369 5369
Size: 5936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 830 4695 4879 830
Size: 3865 184 1887
Fragments arranged by size:
3865 1887 184
MscI TGG'CCA
Cuts at: 313 358 866 1594 3697 313
Size: 45 508 728 2103 2552
Fragments arranged by size:
2552 2103 728 508 45
MslI CAynn'nnrTG
Cuts at: 1054 1645 2062 2185 2443 2891 3925 4052 1054
Size: 591 417 123 258 448 1034 127 2938
Fragments arranged by size:
2938 1034 591 448 417 258 127 123
MspA1I CmG'CkG
Cuts at: 120 198 1010 1257 1527 2230 2553 2846 3047
Size: 78 812 247 270 703 323 293 201
Cuts at: 3047 3361 3721 4505 4750 120
Size: 314 360 784 245 1306
Fragments arranged by size:
1306 812 784 703 360 323 314 293
270 247 245 201 78
NaeI GCC'GGC
Cuts at: 1249 4117 1249
Size: 2868 3068
NarI GG'CG_CC
Cuts at: 214 242 3367 3615 214
Size: 28 3125 248 2535
Fragments arranged by size:
3125 2535 248 28
NcoI C'CATG_G
Cuts at: 107 1037 1223 107
Size: 930 186 4820
Fragments arranged by size:
4820 930 186
NdeI CA'TA_TG
Cuts at: 2063 2063
Size: 5936
NgoMIV G'CCGG_C
Cuts at: 1247 4115 1247
Size: 2868 3068
NspI r_CATG'y
Cuts at: 924 1240 1920 2732 2896 2989 3489 5092 924
Size: 316 680 812 164 93 500 1603 1768
Fragments arranged by size:
1768 1603 812 680 500 316 164 93
PciI A'CATG_T
Cuts at: 920 2892 5088 920
Size: 1972 2196 1768
Fragments arranged by size:
2196 1972 1768
PflMI CCAn_nnn'nTGG
Cuts at: 492 882 1290 492
Size: 390 408 5138
Fragments arranged by size:
5138 408 390
PfoI T'CCnGG_A
Cuts at: 1262 1517 2039 1262
Size: 255 522 5159
Fragments arranged by size:
5159 522 255
PmeI GTTT'AAAC
Cuts at: 2935 2935
Size: 5936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 4382
Size: 32 5904
PpuMI rG'GwC_Cy
Cuts at: 1775 1775
Size: 5936
PspOMI G'GGCC_C
Cuts at: 1156 1156
Size: 5936
PstI C_TGCA'G
Cuts at: 1217 2302 2558 2983 3668 1217
Size: 1085 256 425 685 3485
Fragments arranged by size:
3485 1085 685 425 256
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5936
PvuII CAG'CTG
Cuts at: 1527 2553 2846 3361 3721 1527
Size: 1026 293 515 360 3742
Fragments arranged by size:
3742 1026 515 360 293
RsrII CG'GwC_CG
Cuts at: 629 4131 629
Size: 3502 2434
SacI G_AGCT'C
Cuts at: 127 1078 2609 2950 127
Size: 951 1531 341 3113
Fragments arranged by size:
3113 1531 951 341
SacII CC_GC'GG
Cuts at: 121 121
Size: 5936
SalI G'TCGA_C
Cuts at: 2973 2973
Size: 5936
SapI GCTCTTCn'nnn_
Cuts at: 3959 4169 3959
Size: 210 5726
SbfI CC_TGCA'GG
Cuts at: 2983 2983
Size: 5936
ScaI AGT'ACT
Cuts at: 5453 5453
Size: 5936
SfcI C'TryA_G
Cuts at: 33 1213 1551 2298 2479 2554 2698 2741 2979
Size: 1180 338 747 181 75 144 43 238
Cuts at: 2979 3664 4632 4823 33
Size: 685 968 191 1146
Fragments arranged by size:
1180 1146 968 747 685 338 238 191
181 144 75 43
SfoI GGC'GCC
Cuts at: 215 243 3368 3616 215
Size: 28 3125 248 2535
Fragments arranged by size:
3125 2535 248 28
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5936
SmaI CCC'GGG
Cuts at: 138 219 2248 2958 138
Size: 81 2029 710 3116
Fragments arranged by size:
3116 2029 710 81
SmlI C'TyrA_G
Cuts at: 676 3091 4006 4720 4982 676
Size: 2415 915 714 262 1630
Fragments arranged by size:
2415 1630 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5936
SphI G_CATG'C
Cuts at: 1240 1920 2989 3489 1240
Size: 680 1069 500 3687
Fragments arranged by size:
3687 1069 680 500
SrfI GCCC'GGGC
Cuts at: 138 138
Size: 5936
StyI C'CwwG_G
Cuts at: 107 302 314 546 861 1037 1223 1482 1680
Size: 195 12 232 315 176 186 259 198
Cuts at: 1680 3070 3424 107
Size: 1390 354 2619
Fragments arranged by size:
2619 1390 354 315 259 232 198 195
186 176 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2074 2424 3519 3652 4111 4320 2074
Size: 350 1095 133 459 209 3690
Fragments arranged by size:
3690 1095 459 350 209 133
TatI w'GTAC_w
Cuts at: 1130 1430 1988 2177 5451 1130
Size: 300 558 189 3274 1615
Fragments arranged by size:
3274 1615 558 300 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2891 2923 5453 5485 2891
Size: 32 2530 32 3342
Fragments arranged by size:
3342 2530 32 32
Tth111I GACn'n_nGTC
Cuts at: 3733 3733
Size: 5936
XbaI T'CTAG_A
Cuts at: 2967 2967
Size: 5936
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2832 3431 2832
Size: 599 5337
XmaI C'CCGG_G
Cuts at: 136 217 2246 2956 136
Size: 81 2029 710 3116
Fragments arranged by size:
3116 2029 710 81
XmnI GAAnn'nnTTC
Cuts at: 454 454
Size: 5936
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BbsI BbvCI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BstZ17I Bsu36I ClaI CspCI EcoNI FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SspI StuI SwaI XhoI
ZraI