Restriction map for
pF1KB3085
(Circular) (Six-base) MAPSORT of: pF1KB3085.seq Check: 4252 from: 1 to: 3746
pF1KB3085 3746 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 762 762
Size: 3746
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 202 352 202
Size: 150 3596
AlwNI CAG_nnn'CTG
Cuts at: 1051 2618 1051
Size: 1567 2179
ApaLI G'TGCA_C
Cuts at: 2516 2516
Size: 3746
ApoI r'AATT_y
Cuts at: 547 586 750 3099 3355 547
Size: 39 164 2349 256 938
Fragments arranged by size:
2349 938 256 164 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 766 766
Size: 3746
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 166 643 762 1176 1424 1459 166
Size: 477 119 414 248 35 2453
Fragments arranged by size:
2453 477 414 248 119 35
BanII G_rGCy'C
Cuts at: 760 1790 760
Size: 1030 2716
BbeI G_GCGC'C
Cuts at: 170 1180 1428 170
Size: 1010 248 2488
Fragments arranged by size:
2488 1010 248
BbsI GAAGACnn'nnnn_
Cuts at: 440 440
Size: 3746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 519 553 519
Size: 34 3712
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 1266 1266
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 648 1371 1464 2520 648
Size: 723 93 1056 1874
Fragments arranged by size:
1874 1056 723 93
BmrI ACTGGGnnnn_n'
Cuts at: 186 562 958 1039 1374 3518 186
Size: 376 396 81 335 2144 414
Fragments arranged by size:
2144 414 396 376 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 3746
BsaAI yAC'GTr
Cuts at: 738 1099 738
Size: 361 3385
BsaBI GATnn'nnATC
Cuts at: 6 388 1260 3297 6
Size: 382 872 2037 455
Fragments arranged by size:
2037 872 455 382
BsaHI Gr'CG_yC
Cuts at: 167 1177 1425 2127 3623 167
Size: 1010 248 702 1496 290
Fragments arranged by size:
1496 1010 702 290 248
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2408 2555 2966 935
Size: 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 611 1171 1929 2506 3002 611
Size: 560 758 577 496 1355
Fragments arranged by size:
1355 758 577 560 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 698 466
Size: 232 3514
BsiEI CG_ry'CG
Cuts at: 104 1288 1334 2542 104
Size: 1184 46 1208 1308
Fragments arranged by size:
1308 1208 1184 46
BsiHKAI G_wGCw'C
Cuts at: 283 760 1538 1728 2520 283
Size: 477 778 190 792 1509
Fragments arranged by size:
1509 792 778 477 190
Bsp1286I G_dGCh'C
Cuts at: 283 648 760 1371 1464 1538 1728 1790 2520
Size: 365 112 611 93 74 190 62 730
Cuts at: 2520 283
Size: 1509
Fragments arranged by size:
1509 730 611 365 190 112 93 74
62
BspEI T'CCGG_A
Cuts at: 2966 2966
Size: 3746
BspHI T'CATG_A
Cuts at: 2922 2922
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 484 720 796 1010 1312 1693 484
Size: 236 76 214 302 381 2537
Fragments arranged by size:
2537 381 302 236 214 76
BsrBI CCG'CTC
Cuts at: 50 2038 2092 3576 3741 50
Size: 1988 54 1484 165 55
Fragments arranged by size:
1988 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 334 1658 334
Size: 1324 2422
BsrFI r'CCGG_y
Cuts at: 214 1744 1925 2134 214
Size: 1530 181 209 1826
Fragments arranged by size:
1826 1530 209 181
BssSI C'ACGA_G
Cuts at: 2017 2375 2017
Size: 358 3388
BstAPI GCAn_nnn'nTGC
Cuts at: 349 1123 349
Size: 774 2972
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstYI r'GATC_y
Cuts at: 1 290 771 1238 1261 1596 1842 2854 2940
Size: 289 481 467 23 335 246 1012 86
Cuts at: 2940 2952 3298 1
Size: 12 346 449
Fragments arranged by size:
1012 481 467 449 346 335 289 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3746
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 179 989 179
Size: 810 2936
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 377 412 377
Size: 35 3711
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2310 1452
Size: 858 2888
EaeI y'GGCC_r
Cuts at: 110 398 1331 1505 1896 1923 3268 110
Size: 288 933 174 391 27 1345 588
Fragments arranged by size:
1345 933 588 391 288 174 27
EagI C'GGCC_G
Cuts at: 1331 1331
Size: 3746
EarI CTCTTCn'nnn_
Cuts at: 607 1769 1979 607
Size: 1162 210 2374
Fragments arranged by size:
2374 1162 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1954 2264 2410 3556 1954
Size: 310 146 1146 2144
Fragments arranged by size:
2144 1146 310 146
EcoICRI GAG'CTC
Cuts at: 758 758
Size: 3746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 698 885 698
Size: 187 3559
EcoRI G'AATT_C
Cuts at: 547 750 547
Size: 203 3543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1765 1765
Size: 3746
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 170 1180 1428 2450 3429 170
Size: 1010 248 1022 979 487
Fragments arranged by size:
1022 1010 979 487 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1588 1618 1620 1650 1588
Size: 30 2 30 3684
Fragments arranged by size:
3684 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 166 1176 1424 166
Size: 1010 248 2488
Fragments arranged by size:
2488 1010 248
KpnI G_GTAC'C
Cuts at: 766 766
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2601 2417
Size: 184 3562
MscI TGG'CCA
Cuts at: 112 400 1507 112
Size: 288 1107 2351
Fragments arranged by size:
2351 1107 288
MslI CAynn'nnrTG
Cuts at: 1735 1862 1735
Size: 127 3619
MspA1I CmG'CkG
Cuts at: 129 725 857 1171 1531 2544 2789 129
Size: 596 132 314 360 1013 245 1086
Fragments arranged by size:
1086 1013 596 360 314 245 132
NaeI GCC'GGC
Cuts at: 216 1927 216
Size: 1711 2035
NarI GG'CG_CC
Cuts at: 167 1177 1425 167
Size: 1010 248 2488
Fragments arranged by size:
2488 1010 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3746
NgoMIV G'CCGG_C
Cuts at: 214 1925 214
Size: 1711 2035
NspI r_CATG'y
Cuts at: 799 1299 2206 799
Size: 500 907 2339
Fragments arranged by size:
2339 907 500
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PflMI CCAn_nnn'nTGG
Cuts at: 521 521
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PshAI GACnn'nnGTC
Cuts at: 568 568
Size: 3746
PstI C_TGCA'G
Cuts at: 128 793 1478 128
Size: 665 685 2396
Fragments arranged by size:
2396 685 665
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 129 1171 1531 129
Size: 1042 360 2344
Fragments arranged by size:
2344 1042 360
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 760 760
Size: 3746
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 124 789 1474 2467 2658 33
Size: 91 665 685 993 191 1121
Fragments arranged by size:
1121 993 685 665 191 91
SfoI GGC'GCC
Cuts at: 168 1178 1426 168
Size: 1010 248 2488
Fragments arranged by size:
2488 1010 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 901 1816 2308 2570 901
Size: 915 492 262 2077
Fragments arranged by size:
2077 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
StyI C'CwwG_G
Cuts at: 107 694 880 1234 107
Size: 587 186 354 2619
Fragments arranged by size:
2619 587 354 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1329 1462 1921 2130 1329
Size: 133 459 209 2945
Fragments arranged by size:
2945 459 209 133
TatI w'GTAC_w
Cuts at: 3261 3261
Size: 3746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1241 1241
Size: 3746
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI