Restriction map for
pF1KB3086
(Circular) (Six-base) MAPSORT of: pF1KB3086.seq Check: 8425 from: 1 to: 4853
pF1KB3086 4853 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 958 958
Size: 4853
AccI GT'mk_AC
Cuts at: 1891 1891
Size: 4853
Acc65I G'GTAC_C
Cuts at: 1869 1869
Size: 4853
AclI AA'CG_TT
Cuts at: 1778 4693 1778
Size: 2915 1938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 781 865 1217 1525 1813 2678 3110 3463
Size: 411 84 352 308 288 865 432 353
Cuts at: 3463 4125 370
Size: 662 1098
Fragments arranged by size:
1098 865 662 432 411 353 352 308
288 84
AflIII A'CryG_T
Cuts at: 2205 4005 4286 2205
Size: 1800 281 2772
Fragments arranged by size:
2772 1800 281
AleI CACnn'nnGTG
Cuts at: 559 559
Size: 4853
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 394 426 1768 1800 394
Size: 32 1342 32 3447
Fragments arranged by size:
3447 1342 32 32
AlwNI CAG_nnn'CTG
Cuts at: 240 804 909 1524 2158 3596 240
Size: 564 105 615 634 1438 1497
Fragments arranged by size:
1497 1438 634 615 564 105
ApaI G_GGCC'C
Cuts at: 316 1471 316
Size: 1155 3698
ApaLI G'TGCA_C
Cuts at: 1843 3691 1843
Size: 1848 3005
ApoI r'AATT_y
Cuts at: 547 735 1857 4206 4462 547
Size: 188 1122 2349 256 938
Fragments arranged by size:
2349 1122 938 256 188
AseI AT'TA_AT
Cuts at: 20 20
Size: 4853
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
AvaI C'yCGr_G
Cuts at: 168 1771 1873 168
Size: 1603 102 3148
Fragments arranged by size:
3148 1603 102
BamHI G'GATC_C
Cuts at: 1878 1878
Size: 4853
BanI G'GyrC_C
Cuts at: 261 1869 2283 2531 2566 261
Size: 1608 414 248 35 2548
Fragments arranged by size:
2548 1608 414 248 35
BanII G_rGCy'C
Cuts at: 316 407 1471 1867 2897 316
Size: 91 1064 396 1030 2272
Fragments arranged by size:
2272 1064 1030 396 91
BbeI G_GCGC'C
Cuts at: 2287 2535 2287
Size: 248 4605
BciVI GTATCCnnnnn_n'
Cuts at: 2751 3807 4853 2751
Size: 1056 1046 2751
Fragments arranged by size:
2751 1056 1046
BclI T'GATC_A
Cuts at: 824 837 2373 824
Size: 13 1536 3304
Fragments arranged by size:
3304 1536 13
BglII A'GATC_T
Cuts at: 1180 1200 2368 4405 1180
Size: 20 1168 2037 1628
Fragments arranged by size:
2037 1628 1168 20
BlpI GC'TnA_GC
Cuts at: 154 1965 154
Size: 1811 3042
Bme1580I G_kGCm'C
Cuts at: 316 1158 1471 1847 2478 2571 3695 316
Size: 842 313 376 631 93 1124 1474
Fragments arranged by size:
1474 1124 842 631 376 313 93
BmgBI CAC'GTC
Cuts at: 715 715
Size: 4853
BmrI ACTGGGnnnn_n'
Cuts at: 2065 2146 2481 4625 2065
Size: 81 335 2144 2293
Fragments arranged by size:
2293 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3220 3220
Size: 4853
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1014 1014
Size: 4853
Bpu10I CC'TnA_GC
Cuts at: 1472 1472
Size: 4853
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 312 391 2029 2908 3622 3920 238
Size: 74 79 1638 879 714 298 1171
Fragments arranged by size:
1638 1171 879 714 298 79 74
BsaI GGTCTCn'nnnn_
Cuts at: 1078 4557 1078
Size: 3479 1374
BsaAI yAC'GTr
Cuts at: 2206 2206
Size: 4853
BsaBI GATnn'nnATC
Cuts at: 6 2367 4404 6
Size: 2361 2037 455
Fragments arranged by size:
2361 2037 455
BsaHI Gr'CG_yC
Cuts at: 203 2284 2532 3234 4730 203
Size: 2081 248 702 1496 326
Fragments arranged by size:
2081 1496 702 326 248
BsaWI w'CCGG_w
Cuts at: 2042 2563 3241 3652 3799 4073 2042
Size: 521 678 411 147 274 2822
Fragments arranged by size:
2822 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 394 424 394
Size: 30 4823
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 719 719
Size: 4853
BseYI C'CCAG_C
Cuts at: 647 2278 3036 3701 4109 647
Size: 1631 758 665 408 1391
Fragments arranged by size:
1631 1391 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2395 2441 3671 104
Size: 2291 46 1230 1286
Fragments arranged by size:
2291 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 407 1847 1867 2645 2835 3695 407
Size: 1440 20 778 190 860 1565
Fragments arranged by size:
1565 1440 860 778 190 20
BsmI GAATG_Cn'
Cuts at: 432 875 1122 432
Size: 443 247 4163
Fragments arranged by size:
4163 443 247
Bsp1286I G_dGCh'C
Cuts at: 316 407 1158 1471 1847 1867 2478 2571 2645
Size: 91 751 313 376 20 611 93 74
Cuts at: 2645 2835 2897 3695 316
Size: 190 62 798 1474
Fragments arranged by size:
1474 798 751 611 376 313 190 93
91 74 62 20
BspHI T'CATG_A
Cuts at: 3285 3285
Size: 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 958 1903 2117 2419 2800 958
Size: 945 214 302 381 3011
Fragments arranged by size:
3011 945 381 302 214
BsrBI CCG'CTC
Cuts at: 50 224 3145 3199 4683 4848 50
Size: 174 2921 54 1484 165 55
Fragments arranged by size:
2921 1484 174 165 55 54
BsrDI GCAATG_nn'
Cuts at: 948 2765 948
Size: 1817 3036
BsrFI r'CCGG_y
Cuts at: 2851 3032 2851
Size: 181 4672
BsrGI T'GTAC_A
Cuts at: 1037 1037
Size: 4853
BssSI C'ACGA_G
Cuts at: 1390 3124 3832 1390
Size: 1734 708 2411
Fragments arranged by size:
2411 1734 708
BstAPI GCAn_nnn'nTGC
Cuts at: 131 2230 131
Size: 2099 2754
BstBI TT'CG_AA
Cuts at: 3214 3214
Size: 4853
BstXI CCAn_nnnn'nTGG
Cuts at: 1821 1821
Size: 4853
BstYI r'GATC_y
Cuts at: 1 788 1180 1200 1878 2345 2368 2703 2949
Size: 787 392 20 678 467 23 335 246
Cuts at: 2949 3255 3267 3353 4405 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 787 678 467 449 392 335 306
246 86 23 20 12
Bsu36I CC'TnA_GG
Cuts at: 308 308
Size: 4853
BtgI C'CryG_G
Cuts at: 107 107
Size: 4853
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 160 2803 2984 3147 160
Size: 2643 181 163 1866
Fragments arranged by size:
2643 1866 181 163
BtsI GCAGTG_nn'
Cuts at: 772 2096 772
Size: 1324 3529
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1122 1157 1122
Size: 35 4818
DraI TTT'AAA
Cuts at: 1852 1852
Size: 4853
DraIII CAC_nnn'GTG
Cuts at: 131 131
Size: 4853
DrdI GACnn_nn'nnGTC
Cuts at: 2559 3903 2559
Size: 1344 3509
EaeI y'GGCC_r
Cuts at: 110 864 1811 2438 2612 3003 3030 4375 110
Size: 754 947 627 174 391 27 1345 588
Fragments arranged by size:
1345 947 754 627 588 391 174 27
EagI C'GGCC_G
Cuts at: 2438 2438
Size: 4853
EarI CTCTTCn'nnn_
Cuts at: 2876 3086 2876
Size: 210 4643
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1369 3061 3803 3949 4663 1369
Size: 1692 742 146 714 1559
Fragments arranged by size:
1692 1559 742 714 146
EcoICRI GAG'CTC
Cuts at: 405 1865 405
Size: 1460 3393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 781 865 1014 1217 1525 1813 2678 3110
Size: 411 84 149 203 308 288 865 432
Cuts at: 3110 3463 4125 370
Size: 353 662 1098
Fragments arranged by size:
1098 865 662 432 411 353 308 288
203 149 84
EcoNI CCTnn'n_nnAGG
Cuts at: 1573 1573
Size: 4853
EcoO109I rG'GnC_Cy
Cuts at: 312 1467 1468 1992 312
Size: 1155 1 524 3173
Fragments arranged by size:
3173 1155 524 1
EcoRI G'AATT_C
Cuts at: 1857 1857
Size: 4853
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 4853
FspI TGC'GCA
Cuts at: 124 2634 124
Size: 2510 2343
FspAI rTGC'GCAy
Cuts at: 124 124
Size: 4853
HaeII r_GCGC'y
Cuts at: 2287 2535 3765 4536 2287
Size: 248 1230 771 2604
Fragments arranged by size:
2604 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 2695 2725 2727 2757 1373
Size: 32 1290 30 2 30 3469
Fragments arranged by size:
3469 1290 32 30 30 2
HincII GTy'rAC
Cuts at: 1892 1892
Size: 4853
HindIII A'AGCT_T
Cuts at: 527 745 1795 527
Size: 218 1050 3585
Fragments arranged by size:
3585 1050 218
KasI G'GCGC_C
Cuts at: 2283 2531 2283
Size: 248 4605
KpnI G_GTAC'C
Cuts at: 1873 1873
Size: 4853
MluI A'CGCG_T
Cuts at: 4286 4286
Size: 4853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3612 3796 3612
Size: 184 4669
MscI TGG'CCA
Cuts at: 1813 2614 1813
Size: 801 4052
MslI CAynn'nnrTG
Cuts at: 559 880 1537 1807 1819 2842 2969 559
Size: 321 657 270 12 1023 127 2443
Fragments arranged by size:
2443 1023 657 321 270 127 12
MspA1I CmG'CkG
Cuts at: 381 1791 1964 2278 2638 3422 3667 381
Size: 1410 173 314 360 784 245 1567
Fragments arranged by size:
1567 1410 784 360 314 245 173
NaeI GCC'GGC
Cuts at: 3034 3034
Size: 4853
NarI GG'CG_CC
Cuts at: 2284 2532 2284
Size: 248 4605
NcoI C'CATG_G
Cuts at: 107 107
Size: 4853
NgoMIV G'CCGG_C
Cuts at: 3032 3032
Size: 4853
NspI r_CATG'y
Cuts at: 323 1038 1906 2406 4009 323
Size: 715 868 500 1603 1167
Fragments arranged by size:
1603 1167 868 715 500
PciI A'CATG_T
Cuts at: 4005 4005
Size: 4853
PflMI CCAn_nnn'nTGG
Cuts at: 884 884
Size: 4853
PmeI GTTT'AAAC
Cuts at: 1852 1852
Size: 4853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 394 426 3299 3331 394
Size: 32 2873 32 1916
Fragments arranged by size:
2873 1916 32 32
PspOMI G'GGCC_C
Cuts at: 312 1467 312
Size: 1155 3698
PstI C_TGCA'G
Cuts at: 245 1339 1900 2585 245
Size: 1094 561 685 2513
Fragments arranged by size:
2513 1094 685 561
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4853
PvuII CAG'CTG
Cuts at: 381 2278 2638 381
Size: 1897 360 2596
Fragments arranged by size:
2596 1897 360
RsrII CG'GwC_CG
Cuts at: 3048 3048
Size: 4853
SacI G_AGCT'C
Cuts at: 407 1867 407
Size: 1460 3393
SalI G'TCGA_C
Cuts at: 1890 1890
Size: 4853
SapI GCTCTTCn'nnn_
Cuts at: 2876 3086 2876
Size: 210 4643
SbfI CC_TGCA'GG
Cuts at: 1900 1900
Size: 4853
ScaI AGT'ACT
Cuts at: 991 4370 991
Size: 3379 1474
SfcI C'TryA_G
Cuts at: 33 241 1311 1335 1896 2581 3549 3740 33
Size: 208 1070 24 561 685 968 191 1146
Fragments arranged by size:
1146 1070 968 685 561 208 191 24
SfoI GGC'GCC
Cuts at: 2285 2533 2285
Size: 248 4605
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
SmaI CCC'GGG
Cuts at: 170 1875 170
Size: 1705 3148
SmlI C'TyrA_G
Cuts at: 253 327 406 2008 2923 3637 3899 253
Size: 74 79 1602 915 714 262 1207
Fragments arranged by size:
1602 1207 915 714 262 79 74
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4853
SphI G_CATG'C
Cuts at: 1906 2406 1906
Size: 500 4353
StuI AGG'CCT
Cuts at: 448 665 448
Size: 217 4636
StyI C'CwwG_G
Cuts at: 107 680 1987 2341 107
Size: 573 1307 354 2619
Fragments arranged by size:
2619 1307 573 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 249 532 2436 2569 3028 3237 249
Size: 283 1904 133 459 209 1865
Fragments arranged by size:
1904 1865 459 283 209 133
TatI w'GTAC_w
Cuts at: 989 1037 4368 989
Size: 48 3331 1474
Fragments arranged by size:
3331 1474 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 806 838 4370 4402 806
Size: 32 3532 32 1257
Fragments arranged by size:
3532 1257 32 32
Tth111I GACn'n_nGTC
Cuts at: 604 2650 604
Size: 2046 2807
XbaI T'CTAG_A
Cuts at: 1884 1884
Size: 4853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2348 2348
Size: 4853
XmaI C'CCGG_G
Cuts at: 168 1873 168
Size: 1705 3148
XmnI GAAnn'nnTTC
Cuts at: 1004 1280 1297 1004
Size: 276 17 4560
Fragments arranged by size:
4560 276 17
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BsgI
BsiWI BsmBI BspEI BssHII BstEII BstZ17I ClaI EcoRV
FseI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI