Restriction map for
pF1KB3088
(Circular) (Six-base) MAPSORT of: pF1KB3088.seq Check: 6895 from: 1 to: 4367
pF1KB3088 4367 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 995 1405 995
Size: 410 3957
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 846 2192 2624 2977 3639 846
Size: 1346 432 353 662 1574
Fragments arranged by size:
1574 1346 662 432 353
AflIII A'CryG_T
Cuts at: 1719 3519 3800 1719
Size: 1800 281 2286
Fragments arranged by size:
2286 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 278 1198 1672 3110 278
Size: 920 474 1438 1535
Fragments arranged by size:
1535 1438 920 474
ApaLI G'TGCA_C
Cuts at: 3205 3205
Size: 4367
ApoI r'AATT_y
Cuts at: 1371 3720 3976 1371
Size: 2349 256 1762
Fragments arranged by size:
2349 1762 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 1187 1387 1187
Size: 200 4167
BamHI G'GATC_C
Cuts at: 582 1392 582
Size: 810 3557
BanI G'GyrC_C
Cuts at: 173 247 292 310 387 985 1383 1797 2045
Size: 74 45 18 77 598 398 414 248
Cuts at: 2045 2080 173
Size: 35 2460
Fragments arranged by size:
2460 598 414 398 248 77 74 45
35 18
BanII G_rGCy'C
Cuts at: 347 1381 2411 347
Size: 1034 1030 2303
Fragments arranged by size:
2303 1034 1030
BbeI G_GCGC'C
Cuts at: 296 391 1801 2049 296
Size: 95 1410 248 2614
Fragments arranged by size:
2614 1410 248 95
BbvCI CC'TCA_GC
Cuts at: 169 169
Size: 4367
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3321 4367 2265
Size: 1056 1046 2265
Fragments arranged by size:
2265 1056 1046
BclI T'GATC_A
Cuts at: 525 654 1887 525
Size: 129 1233 3005
Fragments arranged by size:
3005 1233 129
BglI GCCn_nnn'nGGC
Cuts at: 269 269
Size: 4367
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 1992 2085 3209 1992
Size: 93 1124 3150
Fragments arranged by size:
3150 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1579 1660 1995 4139 1579
Size: 81 335 2144 1807
Fragments arranged by size:
2144 1807 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 401 1082 214
Size: 187 681 3499
Fragments arranged by size:
3499 681 187
Bpu10I CC'TnA_GC
Cuts at: 169 1022 169
Size: 853 3514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 1543 2422 3136 3434 584
Size: 959 879 714 298 1517
Fragments arranged by size:
1517 959 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 1720 1720
Size: 4367
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 293 388 1798 2046 2748 4244 293
Size: 95 1410 248 702 1496 416
Fragments arranged by size:
1496 1410 702 416 248 95
BsaWI w'CCGG_w
Cuts at: 585 1556 2077 2755 3166 3313 3587 585
Size: 971 521 678 411 147 274 1365
Fragments arranged by size:
1365 971 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 580 610 580
Size: 30 4337
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 355 388 391 394 355
Size: 33 3 3 4328
Fragments arranged by size:
4328 33 3 3
BseYI C'CCAG_C
Cuts at: 316 1792 2550 3215 3623 316
Size: 1476 758 665 408 1060
Fragments arranged by size:
1476 1060 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 1139 476
Size: 663 3704
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3185 104
Size: 1805 46 1230 1286
Fragments arranged by size:
1805 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 837 1381 2159 2349 3209 837
Size: 544 778 190 860 1995
Fragments arranged by size:
1995 860 778 544 190
Bsp1286I G_dGCh'C
Cuts at: 347 837 1381 1992 2085 2159 2349 2411 3209
Size: 490 544 611 93 74 190 62 798
Cuts at: 3209 347
Size: 1505
Fragments arranged by size:
1505 798 611 544 490 190 93 74
62
BspEI T'CCGG_A
Cuts at: 585 585
Size: 4367
BspHI T'CATG_A
Cuts at: 2799 2799
Size: 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 197 1417 1631 1933 2314 197
Size: 1220 214 302 381 2250
Fragments arranged by size:
2250 1220 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1016 1238 2279 1016
Size: 222 1041 3104
Fragments arranged by size:
3104 1041 222
BsrFI r'CCGG_y
Cuts at: 2365 2546 2365
Size: 181 4186
BsrGI T'GTAC_A
Cuts at: 788 788
Size: 4367
BssHII G'CGCG_C
Cuts at: 253 253
Size: 4367
BssSI C'ACGA_G
Cuts at: 2638 3346 2638
Size: 708 3659
BstAPI GCAn_nnn'nTGC
Cuts at: 1198 1744 1198
Size: 546 3821
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstEII G'GTnAC_C
Cuts at: 855 855
Size: 4367
BstXI CCAn_nnnn'nTGG
Cuts at: 1210 1210
Size: 4367
BstYI r'GATC_y
Cuts at: 1 119 582 1392 1859 1882 2217 2463 2769
Size: 118 463 810 467 23 335 246 306
Cuts at: 2769 2781 2867 3919 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 810 467 463 449 335 306 246
118 86 23 12
BstZ17I GTA'TAC
Cuts at: 996 996
Size: 4367
BtgI C'CryG_G
Cuts at: 182 392 182
Size: 210 4157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1278 2317 2498 2661 1278
Size: 1039 181 163 2984
Fragments arranged by size:
2984 1039 181 163
BtsI GCAGTG_nn'
Cuts at: 815 925 1355 1610 815
Size: 110 430 255 3572
Fragments arranged by size:
3572 430 255 110
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 2073 3417 2073
Size: 1344 3023
EaeI y'GGCC_r
Cuts at: 261 1952 2126 2517 2544 3889 261
Size: 1691 174 391 27 1345 739
Fragments arranged by size:
1691 1345 739 391 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 1103 2390 2600 1103
Size: 1287 210 2870
Fragments arranged by size:
2870 1287 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 3317 3463 4177 2575
Size: 742 146 714 2765
Fragments arranged by size:
2765 742 714 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 401 846 1082 2192 2624 2977 3639 214
Size: 187 445 236 1110 432 353 662 942
Fragments arranged by size:
1110 942 662 445 432 353 236 187
EcoNI CCTnn'n_nnAGG
Cuts at: 226 226
Size: 4367
EcoO109I rG'GnC_Cy
Cuts at: 275 285 1264 1506 275
Size: 10 979 242 3136
Fragments arranged by size:
3136 979 242 10
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4367
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 296 391 1801 2049 3279 4050 296
Size: 95 1410 248 1230 771 613
Fragments arranged by size:
1410 1230 771 613 248 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 481 495 513 527 580 612 998 1030 2209
Size: 14 18 14 53 32 386 32 1179
Cuts at: 2209 2239 2241 2271 481
Size: 30 2 30 2577
Fragments arranged by size:
2577 1179 386 53 32 32 30 30
18 14 14 2
HincII GTy'rAC
Cuts at: 330 1406 330
Size: 1076 3291
HindIII A'AGCT_T
Cuts at: 461 912 461
Size: 451 3916
KasI G'GCGC_C
Cuts at: 292 387 1797 2045 292
Size: 95 1410 248 2614
Fragments arranged by size:
2614 1410 248 95
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 380 711 3126 3310 380
Size: 331 2415 184 1437
Fragments arranged by size:
2415 1437 331 184
MscI TGG'CCA
Cuts at: 2128 2128
Size: 4367
MslI CAynn'nnrTG
Cuts at: 2356 2483 2356
Size: 127 4240
MspA1I CmG'CkG
Cuts at: 184 298 394 1478 1792 2152 2936 3181 184
Size: 114 96 1084 314 360 784 245 1370
Fragments arranged by size:
1370 1084 784 360 314 245 114 96
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 293 388 1798 2046 293
Size: 95 1410 248 2614
Fragments arranged by size:
2614 1410 248 95
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NspI r_CATG'y
Cuts at: 649 1420 1920 3523 649
Size: 771 500 1603 1493
Fragments arranged by size:
1603 1493 771 500
PciI A'CATG_T
Cuts at: 3519 3519
Size: 4367
PfoI T'CCnGG_A
Cuts at: 499 499
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 4335
PpuMI rG'GwC_Cy
Cuts at: 275 285 1264 275
Size: 10 979 3378
Fragments arranged by size:
3378 979 10
PsiI TTA'TAA
Cuts at: 467 467
Size: 4367
PspXI vC'TCGA_Gb
Cuts at: 1187 1187
Size: 4367
PstI C_TGCA'G
Cuts at: 149 209 227 1362 1414 2099 149
Size: 60 18 1135 52 685 2417
Fragments arranged by size:
2417 1135 685 60 52 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 1792 2152 1792
Size: 360 4007
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SacII CC_GC'GG
Cuts at: 185 395 185
Size: 210 4157
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 1153 3884 1153
Size: 2731 1636
SfcI C'TryA_G
Cuts at: 33 145 205 223 1358 1410 2095 3063 3254
Size: 112 60 18 1135 52 685 968 191
Cuts at: 3254 33
Size: 1146
Fragments arranged by size:
1146 1135 968 685 191 112 60 52
18
SfoI GGC'GCC
Cuts at: 294 389 1799 2047 294
Size: 95 1410 248 2614
Fragments arranged by size:
2614 1410 248 95
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 599 1187 1522 2437 3151 3413 599
Size: 588 335 915 714 262 1553
Fragments arranged by size:
1553 915 714 588 335 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StyI C'CwwG_G
Cuts at: 1501 1855 1501
Size: 354 4013
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 813 1950 2083 2542 2751 813
Size: 1137 133 459 209 2429
Fragments arranged by size:
2429 1137 459 209 133
TatI w'GTAC_w
Cuts at: 788 1151 3882 788
Size: 363 2731 1273
Fragments arranged by size:
2731 1273 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 917 949 3884 3916 917
Size: 32 2935 32 1368
Fragments arranged by size:
2935 1368 32 32
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XhoI C'TCGA_G
Cuts at: 1187 1187
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
XmnI GAAnn'nnTTC
Cuts at: 1256 1256
Size: 4367
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PsiI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BcgI BfrBI
BmgBI BmtI BsiWI BsmI BsmBI Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PspOMI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI