Restriction map for
pF1KB3089
(Circular) (Six-base) MAPSORT of: pF1KB3089.seq Check: 7374 from: 1 to: 4538
pF1KB3089 4538 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1491 1491
Size: 4538
AatII G_ACGT'C
Cuts at: 731 731
Size: 4538
AccI GT'mk_AC
Cuts at: 725 1576 725
Size: 851 3687
Acc65I G'GTAC_C
Cuts at: 1554 1554
Size: 4538
AclI AA'CG_TT
Cuts at: 4378 4378
Size: 4538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 1117 1271 2363 2795 3148 3810 266
Size: 851 154 1092 432 353 662 994
Fragments arranged by size:
1092 994 851 662 432 353 154
AfeI AGC'GCT
Cuts at: 916 916
Size: 4538
AflIII A'CryG_T
Cuts at: 1890 3690 3971 1890
Size: 1800 281 2457
Fragments arranged by size:
2457 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 447 447
Size: 4538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 4538
AlwNI CAG_nnn'CTG
Cuts at: 159 1843 3281 159
Size: 1684 1438 1416
Fragments arranged by size:
1684 1438 1416
ApaLI G'TGCA_C
Cuts at: 3376 3376
Size: 4538
ApoI r'AATT_y
Cuts at: 226 1542 3891 4147 226
Size: 1316 2349 256 617
Fragments arranged by size:
2349 1316 617 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4538
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
AvaI C'yCGr_G
Cuts at: 690 1558 690
Size: 868 3670
BamHI G'GATC_C
Cuts at: 1563 1563
Size: 4538
BanI G'GyrC_C
Cuts at: 381 1210 1479 1554 1968 2216 2251 381
Size: 829 269 75 414 248 35 2668
Fragments arranged by size:
2668 829 414 269 248 75 35
BanII G_rGCy'C
Cuts at: 459 594 1010 1552 2582 459
Size: 135 416 542 1030 2415
Fragments arranged by size:
2415 1030 542 416 135
BbeI G_GCGC'C
Cuts at: 1972 2220 1972
Size: 248 4290
BbsI GAAGACnn'nnnn_
Cuts at: 254 1105 1119 254
Size: 851 14 3673
Fragments arranged by size:
3673 851 14
BbvCI CC'TCA_GC
Cuts at: 257 257
Size: 4538
BciVI GTATCCnnnnn_n'
Cuts at: 571 2436 3492 4538 571
Size: 1865 1056 1046 571
Fragments arranged by size:
1865 1056 1046 571
BclI T'GATC_A
Cuts at: 2058 2058
Size: 4538
BglII A'GATC_T
Cuts at: 2053 4090 2053
Size: 2037 2501
BlpI GC'TnA_GC
Cuts at: 1650 1650
Size: 4538
Bme1580I G_kGCm'C
Cuts at: 2163 2256 3380 2163
Size: 93 1124 3321
Fragments arranged by size:
3321 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 466 1750 1831 2166 4310 466
Size: 1284 81 335 2144 694
Fragments arranged by size:
2144 1284 694 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1330 2905 1330
Size: 1575 2963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 136
Size: 4538
Bpu10I CC'TnA_GC
Cuts at: 257 458 257
Size: 201 4337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1035 1714 2593 3307 3605 1035
Size: 679 879 714 298 1968
Fragments arranged by size:
1968 879 714 679 298
BsaI GGTCTCn'nnnn_
Cuts at: 4242 4242
Size: 4538
BsaAI yAC'GTr
Cuts at: 1891 1891
Size: 4538
BsaBI GATnn'nnATC
Cuts at: 6 2052 4089 6
Size: 2046 2037 455
Fragments arranged by size:
2046 2037 455
BsaHI Gr'CG_yC
Cuts at: 728 1969 2217 2919 4415 728
Size: 1241 248 702 1496 851
Fragments arranged by size:
1496 1241 851 702 248
BsaWI w'CCGG_w
Cuts at: 1727 2248 2926 3337 3484 3758 1727
Size: 521 678 411 147 274 2507
Fragments arranged by size:
2507 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 526 556 901 931 1441 1471 526
Size: 30 345 30 510 30 3593
Fragments arranged by size:
3593 510 345 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 553 550
Size: 3 4535
BseYI C'CCAG_C
Cuts at: 1963 2721 3386 3794 1963
Size: 758 665 408 2707
Fragments arranged by size:
2707 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 999 999
Size: 4538
BsiEI CG_ry'CG
Cuts at: 104 2080 2126 3356 104
Size: 1976 46 1230 1286
Fragments arranged by size:
1976 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 907 1552 2330 2520 3380 907
Size: 645 778 190 860 2065
Fragments arranged by size:
2065 860 778 645 190
Bsp1286I G_dGCh'C
Cuts at: 459 594 907 1010 1552 2163 2256 2330 2520
Size: 135 313 103 542 611 93 74 190
Cuts at: 2520 2582 3380 459
Size: 62 798 1617
Fragments arranged by size:
1617 798 611 542 313 190 135 103
93 74 62
BspHI T'CATG_A
Cuts at: 2970 2970
Size: 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1491 1588 1802 2104 2485 1491
Size: 97 214 302 381 3544
Fragments arranged by size:
3544 381 302 214 97
BsrBI CCG'CTC
Cuts at: 50 2830 2884 4368 4533 50
Size: 2780 54 1484 165 55
Fragments arranged by size:
2780 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 605 2450 605
Size: 1845 2693
BsrFI r'CCGG_y
Cuts at: 2536 2717 2536
Size: 181 4357
BsrGI T'GTAC_A
Cuts at: 404 404
Size: 4538
BssSI C'ACGA_G
Cuts at: 127 910 2809 3517 127
Size: 783 1899 708 1148
Fragments arranged by size:
1899 1148 783 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1915 1915
Size: 4538
BstBI TT'CG_AA
Cuts at: 2899 2899
Size: 4538
BstXI CCAn_nnnn'nTGG
Cuts at: 498 601 498
Size: 103 4435
BstYI r'GATC_y
Cuts at: 1 1563 2030 2053 2388 2634 2940 2952 3038
Size: 1562 467 23 335 246 306 12 86
Cuts at: 3038 4090 1
Size: 1052 449
Fragments arranged by size:
1562 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 235 235
Size: 4538
BtgI C'CryG_G
Cuts at: 107 171 296 345 479 1214 107
Size: 64 125 49 134 735 3431
Fragments arranged by size:
3431 735 134 125 64 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2488 2669 2832 2488
Size: 181 163 4194
Fragments arranged by size:
4194 181 163
BtsI GCAGTG_nn'
Cuts at: 1781 1781
Size: 4538
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 374 409 374
Size: 35 4503
DraI TTT'AAA
Cuts at: 1537 1537
Size: 4538
DrdI GACnn_nn'nnGTC
Cuts at: 427 2244 3588 427
Size: 1817 1344 1377
Fragments arranged by size:
1817 1377 1344
EaeI y'GGCC_r
Cuts at: 140 182 395 1217 2123 2297 2688 2715 4060
Size: 42 213 822 906 174 391 27 1345
Cuts at: 4060 140
Size: 618
Fragments arranged by size:
1345 906 822 618 391 213 174 42
27
EagI C'GGCC_G
Cuts at: 2123 2123
Size: 4538
EarI CTCTTCn'nnn_
Cuts at: 195 934 1330 2561 2771 195
Size: 739 396 1231 210 1962
Fragments arranged by size:
1962 1231 739 396 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2746 3488 3634 4348 2746
Size: 742 146 714 2936
Fragments arranged by size:
2936 742 714 146
EcoICRI GAG'CTC
Cuts at: 1550 1550
Size: 4538
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 266 1117 1271 2363 2795 3148 3810 136
Size: 130 851 154 1092 432 353 662 864
Fragments arranged by size:
1092 864 851 662 432 353 154 130
EcoO109I rG'GnC_Cy
Cuts at: 239 752 1677 239
Size: 513 925 3100
Fragments arranged by size:
3100 925 513
EcoRI G'AATT_C
Cuts at: 1542 1542
Size: 4538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1011 1452 2557 1011
Size: 441 1105 2992
Fragments arranged by size:
2992 1105 441
FspI TGC'GCA
Cuts at: 2319 2319
Size: 4538
HaeII r_GCGC'y
Cuts at: 918 1972 2220 3450 4221 918
Size: 1054 248 1230 771 1235
Fragments arranged by size:
1235 1230 1054 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 194 226 433 465 836 868 1092 1124 1441
Size: 32 207 32 371 32 224 32 317
Cuts at: 1441 1473 2380 2410 2412 2442 194
Size: 32 907 30 2 30 2290
Fragments arranged by size:
2290 907 371 317 224 207 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 636 798 1577 636
Size: 162 779 3597
Fragments arranged by size:
3597 779 162
KasI G'GCGC_C
Cuts at: 1968 2216 1968
Size: 248 4290
KpnI G_GTAC'C
Cuts at: 1558 1558
Size: 4538
MluI A'CGCG_T
Cuts at: 3971 3971
Size: 4538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3297 3481 3297
Size: 184 4354
MscI TGG'CCA
Cuts at: 2299 2299
Size: 4538
MslI CAynn'nnrTG
Cuts at: 436 2527 2654 436
Size: 2091 127 2320
Fragments arranged by size:
2320 2091 127
MspA1I CmG'CkG
Cuts at: 173 256 481 661 1034 1649 1963 2323 3107
Size: 83 225 180 373 615 314 360 784
Cuts at: 3107 3352 173
Size: 245 1359
Fragments arranged by size:
1359 784 615 373 360 314 245 225
180 83
NaeI GCC'GGC
Cuts at: 2719 2719
Size: 4538
NarI GG'CG_CC
Cuts at: 1969 2217 1969
Size: 248 4290
NcoI C'CATG_G
Cuts at: 107 345 107
Size: 238 4300
NgoMIV G'CCGG_C
Cuts at: 2717 2717
Size: 4538
NspI r_CATG'y
Cuts at: 796 1591 2091 3694 796
Size: 795 500 1603 1640
Fragments arranged by size:
1640 1603 795 500
PciI A'CATG_T
Cuts at: 3690 3690
Size: 4538
PflMI CCAn_nnn'nTGG
Cuts at: 573 573
Size: 4538
PfoI T'CCnGG_A
Cuts at: 279 279
Size: 4538
PmeI GTTT'AAAC
Cuts at: 1537 1537
Size: 4538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 4506
PpuMI rG'GwC_Cy
Cuts at: 239 752 239
Size: 513 4025
PstI C_TGCA'G
Cuts at: 1585 2270 1585
Size: 685 3853
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4538
PvuII CAG'CTG
Cuts at: 256 661 1963 2323 256
Size: 405 1302 360 2471
Fragments arranged by size:
2471 1302 405 360
RsrII CG'GwC_CG
Cuts at: 2733 2733
Size: 4538
SacI G_AGCT'C
Cuts at: 1552 1552
Size: 4538
SacII CC_GC'GG
Cuts at: 174 482 174
Size: 308 4230
SalI G'TCGA_C
Cuts at: 1575 1575
Size: 4538
SanDI GG'GwC_CC
Cuts at: 752 752
Size: 4538
SapI GCTCTTCn'nnn_
Cuts at: 2561 2771 2561
Size: 210 4328
SbfI CC_TGCA'GG
Cuts at: 1585 1585
Size: 4538
ScaI AGT'ACT
Cuts at: 4055 4055
Size: 4538
SfcI C'TryA_G
Cuts at: 33 972 1581 2266 3234 3425 33
Size: 939 609 685 968 191 1146
Fragments arranged by size:
1146 968 939 685 609 191
SfoI GGC'GCC
Cuts at: 1970 2218 1970
Size: 248 4290
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
SmaI CCC'GGG
Cuts at: 1560 1560
Size: 4538
SmlI C'TyrA_G
Cuts at: 1014 1693 2608 3322 3584 1014
Size: 679 915 714 262 1968
Fragments arranged by size:
1968 915 714 679 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4538
SphI G_CATG'C
Cuts at: 1591 2091 1591
Size: 500 4038
StuI AGG'CCT
Cuts at: 970 1189 970
Size: 219 4319
StyI C'CwwG_G
Cuts at: 107 345 585 986 1672 2026 107
Size: 238 240 401 686 354 2619
Fragments arranged by size:
2619 686 401 354 240 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 369 2121 2254 2713 2922 369
Size: 1752 133 459 209 1985
Fragments arranged by size:
1985 1752 459 209 133
TatI w'GTAC_w
Cuts at: 404 4053 404
Size: 3649 889
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 494 526 901 933 1152 1184 4055 4087 494
Size: 32 375 32 219 32 2871 32 945
Fragments arranged by size:
2871 945 375 219 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 562 2335 562
Size: 1773 2765
XbaI T'CTAG_A
Cuts at: 1569 1569
Size: 4538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 432 784 2033 432
Size: 352 1249 2937
Fragments arranged by size:
2937 1249 352
XmaI C'CCGG_G
Cuts at: 1558 1558
Size: 4538
ZraI GAC'GTC
Cuts at: 729 729
Size: 4538
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AflII AgeI AleI AloI ApaI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI BsmBI
BspEI BssHII BstEII BstZ17I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI