Restriction map for
pF1KB3091
(Circular) (Six-base) MAPSORT of: pF1KB3091.seq Check: 3679 from: 1 to: 5915
pF1KB3091 5915 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AccI GT'mk_AC
Cuts at: 1795 2072 2953 1795
Size: 277 881 4757
Fragments arranged by size:
4757 881 277
Acc65I G'GTAC_C
Cuts at: 2931 2931
Size: 5915
AclI AA'CG_TT
Cuts at: 5755 5755
Size: 5915
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 978 1847 2549 2867 3740 4172 4525 5187 978
Size: 869 702 318 873 432 353 662 1706
Fragments arranged by size:
1706 873 869 702 662 432 353 318
AflIII A'CryG_T
Cuts at: 1937 2508 3267 5067 5348 1937
Size: 571 759 1800 281 2504
Fragments arranged by size:
2504 1800 759 571 281
AgeI A'CCGG_T
Cuts at: 833 833
Size: 5915
AleI CACnn'nnGTG
Cuts at: 205 205
Size: 5915
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2739 2739
Size: 5915
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1940 1972 1940
Size: 32 5883
AlwNI CAG_nnn'CTG
Cuts at: 1083 1368 3220 4658 1083
Size: 285 1852 1438 2340
Fragments arranged by size:
2340 1852 1438 285
ApaLI G'TGCA_C
Cuts at: 1655 4753 1655
Size: 3098 2817
ApoI r'AATT_y
Cuts at: 357 637 770 1188 1507 1756 2919 5268 5524
Size: 280 133 418 319 249 1163 2349 256
Cuts at: 5524 357
Size: 748
Fragments arranged by size:
2349 1163 748 418 319 280 256 249
133
AseI AT'TA_AT
Cuts at: 20 1594 2338 20
Size: 1574 744 3597
Fragments arranged by size:
3597 1574 744
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5915
AvaI C'yCGr_G
Cuts at: 269 2237 2935 269
Size: 1968 698 3249
Fragments arranged by size:
3249 1968 698
BamHI G'GATC_C
Cuts at: 2940 2940
Size: 5915
BanI G'GyrC_C
Cuts at: 2931 3345 3593 3628 2931
Size: 414 248 35 5218
Fragments arranged by size:
5218 414 248 35
BanII G_rGCy'C
Cuts at: 1035 2929 3959 1035
Size: 1894 1030 2991
Fragments arranged by size:
2991 1894 1030
BbeI G_GCGC'C
Cuts at: 3349 3597 3349
Size: 248 5667
BbsI GAAGACnn'nnnn_
Cuts at: 509 509
Size: 5915
BciVI GTATCCnnnnn_n'
Cuts at: 3813 4869 5915 3813
Size: 1056 1046 3813
Fragments arranged by size:
3813 1056 1046
BclI T'GATC_A
Cuts at: 296 651 776 2574 3435 296
Size: 355 125 1798 861 2776
Fragments arranged by size:
2776 1798 861 355 125
BglII A'GATC_T
Cuts at: 378 1263 1611 3430 5467 378
Size: 885 348 1819 2037 826
Fragments arranged by size:
2037 1819 885 826 348
BlpI GC'TnA_GC
Cuts at: 1907 3027 1907
Size: 1120 4795
Bme1580I G_kGCm'C
Cuts at: 1659 3540 3633 4757 1659
Size: 1881 93 1124 2817
Fragments arranged by size:
2817 1881 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2162 3127 3208 3543 5687 2162
Size: 965 81 335 2144 2390
Fragments arranged by size:
2390 2144 965 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4282 4282
Size: 5915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 749
Size: 5915
Bpu10I CC'TnA_GC
Cuts at: 2618 2618
Size: 5915
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1100 2680 3091 3970 4684 4982 688
Size: 412 1580 411 879 714 298 1621
Fragments arranged by size:
1621 1580 879 714 412 411 298
BsaI GGTCTCn'nnnn_
Cuts at: 2527 5619 2527
Size: 3092 2823
BsaAI yAC'GTr
Cuts at: 1938 3268 1938
Size: 1330 4585
BsaBI GATnn'nnATC
Cuts at: 6 650 1487 1535 3429 5466 6
Size: 644 837 48 1894 2037 455
Fragments arranged by size:
2037 1894 837 644 455 48
BsaHI Gr'CG_yC
Cuts at: 3346 3594 4296 5792 3346
Size: 248 702 1496 3469
Fragments arranged by size:
3469 1496 702 248
BsaWI w'CCGG_w
Cuts at: 833 3104 3625 4303 4714 4861 5135 833
Size: 2271 521 678 411 147 274 1613
Fragments arranged by size:
2271 1613 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1940 1970 2656 2686 2755 2785 1940
Size: 30 686 30 69 30 5070
Fragments arranged by size:
5070 686 69 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 667 731 779 1192 1412 2677 667
Size: 64 48 413 220 1265 3905
Fragments arranged by size:
3905 1265 413 220 64 48
BseYI C'CCAG_C
Cuts at: 3340 4098 4763 5171 3340
Size: 758 665 408 4084
Fragments arranged by size:
4084 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 1622 136
Size: 1486 4429
BsiEI CG_ry'CG
Cuts at: 104 152 222 3457 3503 4733 104
Size: 48 70 3235 46 1230 1286
Fragments arranged by size:
3235 1286 1230 70 48 46
BsiHKAI G_wGCw'C
Cuts at: 1659 1953 2929 3707 3897 4757 1659
Size: 294 976 778 190 860 2817
Fragments arranged by size:
2817 976 860 778 294 190
BsmBI CGTCTCn'nnnn_
Cuts at: 121 121
Size: 5915
Bsp1286I G_dGCh'C
Cuts at: 1035 1659 1953 2929 3540 3633 3707 3897 3959
Size: 624 294 976 611 93 74 190 62
Cuts at: 3959 4757 1035
Size: 798 2193
Fragments arranged by size:
2193 976 798 624 611 294 190 93
74 62
BspHI T'CATG_A
Cuts at: 2460 4347 2460
Size: 1887 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2965 3179 3481 3862 2965
Size: 214 302 381 5018
Fragments arranged by size:
5018 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4207 4261 5745 5910 50
Size: 4157 54 1484 165 55
Fragments arranged by size:
4157 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2414 3827 2414
Size: 1413 4502
BsrFI r'CCGG_y
Cuts at: 312 833 3913 4094 312
Size: 521 3080 181 2133
Fragments arranged by size:
3080 2133 521 181
BssSI C'ACGA_G
Cuts at: 4186 4894 4186
Size: 708 5207
BstAPI GCAn_nnn'nTGC
Cuts at: 2605 3292 2605
Size: 687 5228
BstBI TT'CG_AA
Cuts at: 4276 4276
Size: 5915
BstXI CCAn_nnnn'nTGG
Cuts at: 1747 2283 1747
Size: 536 5379
BstYI r'GATC_y
Cuts at: 1 378 1263 1611 2940 3407 3430 3765 4011
Size: 377 885 348 1329 467 23 335 246
Cuts at: 4011 4317 4329 4415 5467 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1329 1052 885 467 449 377 348 335
306 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1796 2073 1796
Size: 277 5638
Bsu36I CC'TnA_GG
Cuts at: 2314 2314
Size: 5915
BtgI C'CryG_G
Cuts at: 107 107
Size: 5915
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3865 4046 4209 3865
Size: 181 163 5571
Fragments arranged by size:
5571 181 163
BtsI GCAGTG_nn'
Cuts at: 3158 3158
Size: 5915
DraI TTT'AAA
Cuts at: 1784 2914 1784
Size: 1130 4785
DrdI GACnn_nn'nnGTC
Cuts at: 3621 4965 3621
Size: 1344 4571
EaeI y'GGCC_r
Cuts at: 149 263 576 2477 3500 3674 4065 4092 5437
Size: 114 313 1901 1023 174 391 27 1345
Cuts at: 5437 149
Size: 627
Fragments arranged by size:
1901 1345 1023 627 391 313 174 114
27
EagI C'GGCC_G
Cuts at: 149 3500 149
Size: 3351 2564
EarI CTCTTCn'nnn_
Cuts at: 1784 2355 2525 3938 4148 1784
Size: 571 170 1413 210 3551
Fragments arranged by size:
3551 1413 571 210 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4123 4865 5011 5725 4123
Size: 742 146 714 4313
Fragments arranged by size:
4313 742 714 146
EcoICRI GAG'CTC
Cuts at: 2927 2927
Size: 5915
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 978 1847 2549 2867 3740 4172 4525 5187
Size: 229 869 702 318 873 432 353 662
Cuts at: 5187 749
Size: 1477
Fragments arranged by size:
1477 873 869 702 662 432 353 318
229
EcoNI CCTnn'n_nnAGG
Cuts at: 1045 2773 1045
Size: 1728 4187
EcoO109I rG'GnC_Cy
Cuts at: 825 3054 825
Size: 2229 3686
EcoRI G'AATT_C
Cuts at: 637 770 1507 2919 637
Size: 133 737 1412 3633
Fragments arranged by size:
3633 1412 737 133
EcoRV GAT'ATC
Cuts at: 1737 2700 1737
Size: 963 4952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 400 1264 2384 3934 400
Size: 864 1120 1550 2381
Fragments arranged by size:
2381 1550 1120 864
FspI TGC'GCA
Cuts at: 3696 3696
Size: 5915
HaeII r_GCGC'y
Cuts at: 3349 3597 4827 5598 3349
Size: 248 1230 771 3666
Fragments arranged by size:
3666 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 643 675 2684 2716 2755 2787 3757 3787 3789
Size: 32 2009 32 39 32 970 30 2
Cuts at: 3789 3819 643
Size: 30 2739
Fragments arranged by size:
2739 2009 970 39 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1134 1589 2954 1134
Size: 455 1365 4095
Fragments arranged by size:
4095 1365 455
HindIII A'AGCT_T
Cuts at: 1199 1199
Size: 5915
HpaI GTT'AAC
Cuts at: 1134 1134
Size: 5915
KasI G'GCGC_C
Cuts at: 3345 3593 3345
Size: 248 5667
KpnI G_GTAC'C
Cuts at: 2935 2935
Size: 5915
MfeI C'AATT_G
Cuts at: 2260 2260
Size: 5915
MluI A'CGCG_T
Cuts at: 5348 5348
Size: 5915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 119 570 983 2276 4674 4858 119
Size: 451 413 1293 2398 184 1176
Fragments arranged by size:
2398 1293 1176 451 413 184
MscI TGG'CCA
Cuts at: 578 2479 3676 578
Size: 1901 1197 2817
Fragments arranged by size:
2817 1901 1197
MslI CAynn'nnrTG
Cuts at: 112 205 2027 2281 2459 3904 4031 112
Size: 93 1822 254 178 1445 127 1996
Fragments arranged by size:
1996 1822 1445 254 178 127 93
MspA1I CmG'CkG
Cuts at: 3026 3340 3700 4484 4729 3026
Size: 314 360 784 245 4212
Fragments arranged by size:
4212 784 360 314 245
NaeI GCC'GGC
Cuts at: 314 4096 314
Size: 3782 2133
NarI GG'CG_CC
Cuts at: 3346 3594 3346
Size: 248 5667
NcoI C'CATG_G
Cuts at: 107 107
Size: 5915
NdeI CA'TA_TG
Cuts at: 1962 1962
Size: 5915
NgoMIV G'CCGG_C
Cuts at: 312 4094 312
Size: 3782 2133
NspI r_CATG'y
Cuts at: 186 2026 2445 2512 2626 2968 3468 5071 186
Size: 1840 419 67 114 342 500 1603 1030
Fragments arranged by size:
1840 1603 1030 500 419 342 114 67
PasI CC'CwG_GG
Cuts at: 288 288
Size: 5915
PciI A'CATG_T
Cuts at: 2508 5067 2508
Size: 2559 3356
PmeI GTTT'AAAC
Cuts at: 2914 2914
Size: 5915
PmlI CAC'GTG
Cuts at: 1938 1938
Size: 5915
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1940 1972 4361 4393 1940
Size: 32 2389 32 3462
Fragments arranged by size:
3462 2389 32 32
PpuMI rG'GwC_Cy
Cuts at: 825 825
Size: 5915
PsiI TTA'TAA
Cuts at: 416 416
Size: 5915
PstI C_TGCA'G
Cuts at: 137 335 2962 3647 137
Size: 198 2627 685 2405
Fragments arranged by size:
2627 2405 685 198
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5915
PvuII CAG'CTG
Cuts at: 3340 3700 3340
Size: 360 5555
RsrII CG'GwC_CG
Cuts at: 4110 4110
Size: 5915
SacI G_AGCT'C
Cuts at: 2929 2929
Size: 5915
SalI G'TCGA_C
Cuts at: 2952 2952
Size: 5915
SapI GCTCTTCn'nnn_
Cuts at: 3938 4148 3938
Size: 210 5705
SbfI CC_TGCA'GG
Cuts at: 2962 2962
Size: 5915
ScaI AGT'ACT
Cuts at: 1728 2165 5432 1728
Size: 437 3267 2211
Fragments arranged by size:
3267 2211 437
SfcI C'TryA_G
Cuts at: 33 133 331 1730 2958 3643 4611 4802 33
Size: 100 198 1399 1228 685 968 191 1146
Fragments arranged by size:
1399 1228 1146 968 685 198 191 100
SfoI GGC'GCC
Cuts at: 3347 3595 3347
Size: 248 5667
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5915
SmaI CCC'GGG
Cuts at: 271 2937 271
Size: 2666 3249
SmlI C'TyrA_G
Cuts at: 667 1115 2659 3070 3985 4699 4961 667
Size: 448 1544 411 915 714 262 1621
Fragments arranged by size:
1621 1544 915 714 448 411 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5915
SphI G_CATG'C
Cuts at: 186 2026 2968 3468 186
Size: 1840 942 500 2633
Fragments arranged by size:
2633 1840 942 500
SspI AAT'ATT
Cuts at: 1857 1857
Size: 5915
StyI C'CwwG_G
Cuts at: 107 443 2474 2774 3049 3403 107
Size: 336 2031 300 275 354 2619
Fragments arranged by size:
2619 2031 354 336 300 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 541 2171 3498 3631 4090 4299 541
Size: 1630 1327 133 459 209 2157
Fragments arranged by size:
2157 1630 1327 459 209 133
TatI w'GTAC_w
Cuts at: 302 1726 2163 5430 302
Size: 1424 437 3267 787
Fragments arranged by size:
3267 1424 787 437
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5432 5464 5432
Size: 32 5883
Tth111I GACn'n_nGTC
Cuts at: 3712 3712
Size: 5915
XbaI T'CTAG_A
Cuts at: 2946 2946
Size: 5915
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 546 586 2107 3410 546
Size: 40 1521 1303 3051
Fragments arranged by size:
3051 1521 1303 40
XmaI C'CCGG_G
Cuts at: 269 2935 269
Size: 2666 3249
XmnI GAAnn'nnTTC
Cuts at: 506 506
Size: 5915
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PmeI PmlI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BspEI BsrGI BssHII BstEII ClaI CspCI DraIII
FseI FspAI NheI NotI NruI NsiI PacI PflMI
PfoI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI