(Circular) (Six-base) MAPSORT of: pF1KB3092.seq Check: 7977 from: 1 to: 5261 pF1KB3092 5261 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AatII G_ACGT'C Cuts at: 588 588 Size: 5261 AccI GT'mk_AC Cuts at: 595 2299 595 Size: 1704 3557 Acc65I G'GTAC_C Cuts at: 2277 2277 Size: 5261 AclI AA'CG_TT Cuts at: 5101 5101 Size: 5261 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 508 740 3086 3518 3871 4533 508 Size: 232 2346 432 353 662 1236 Fragments arranged by size: 2346 1236 662 432 353 232 AflII C'TTAA_G Cuts at: 288 288 Size: 5261 AflIII A'CryG_T Cuts at: 954 1824 2613 4413 4694 954 Size: 870 789 1800 281 1521 Fragments arranged by size: 1800 1521 870 789 281 AhdI GACnn_n'nnGTC Cuts at: 584 584 Size: 5261 AlwNI CAG_nnn'CTG Cuts at: 1084 2566 4004 1084 Size: 1482 1438 2341 Fragments arranged by size: 2341 1482 1438 ApaLI G'TGCA_C Cuts at: 4099 4099 Size: 5261 ApoI r'AATT_y Cuts at: 1003 1024 1058 1156 1478 1664 2008 2265 4614 Size: 21 34 98 322 186 344 257 2349 Cuts at: 4614 4870 1003 Size: 256 1394 Fragments arranged by size: 2349 1394 344 322 257 256 186 98 34 21 AseI AT'TA_AT Cuts at: 20 20 Size: 5261 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5261 AvaI C'yCGr_G Cuts at: 551 1103 1541 2281 551 Size: 552 438 740 3531 Fragments arranged by size: 3531 740 552 438 BamHI G'GATC_C Cuts at: 1150 2286 1150 Size: 1136 4125 BanI G'GyrC_C Cuts at: 2277 2691 2939 2974 2277 Size: 414 248 35 4564 Fragments arranged by size: 4564 414 248 35 BanII G_rGCy'C Cuts at: 501 1872 2275 3305 501 Size: 1371 403 1030 2457 Fragments arranged by size: 2457 1371 1030 403 BbeI G_GCGC'C Cuts at: 2695 2943 2695 Size: 248 5013 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 111 145 111 Size: 34 5227 BciVI GTATCCnnnnn_n' Cuts at: 3159 4215 5261 3159 Size: 1056 1046 3159 Fragments arranged by size: 3159 1056 1046 BclI T'GATC_A Cuts at: 330 1512 2781 330 Size: 1182 1269 2810 Fragments arranged by size: 2810 1269 1182 BfrBI ATG'CAT Cuts at: 736 1293 736 Size: 557 4704 BglI GCCn_nnn'nGGC Cuts at: 1809 1809 Size: 5261 BglII A'GATC_T Cuts at: 519 2776 4813 519 Size: 2257 2037 967 Fragments arranged by size: 2257 2037 967 BlpI GC'TnA_GC Cuts at: 2373 2373 Size: 5261 Bme1580I G_kGCm'C Cuts at: 2886 2979 4103 2886 Size: 93 1124 4044 Fragments arranged by size: 4044 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 2473 2554 2889 5033 2473 Size: 81 335 2144 2701 Fragments arranged by size: 2701 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3628 3628 Size: 5261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 574 1666 574 Size: 1092 4169 Bpu10I CC'TnA_GC Cuts at: 251 251 Size: 5261 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 758 2251 2437 3316 4030 4328 758 Size: 1493 186 879 714 298 1691 Fragments arranged by size: 1691 1493 879 714 298 186 BsaI GGTCTCn'nnnn_ Cuts at: 4965 4965 Size: 5261 BsaAI yAC'GTr Cuts at: 2614 2614 Size: 5261 BsaBI GATnn'nnATC Cuts at: 6 2775 4812 6 Size: 2769 2037 455 Fragments arranged by size: 2769 2037 455 BsaHI Gr'CG_yC Cuts at: 585 2692 2940 3642 5138 585 Size: 2107 248 702 1496 708 Fragments arranged by size: 2107 1496 708 702 248 BsaWI w'CCGG_w Cuts at: 890 1561 2450 2971 3649 4060 4207 4481 890 Size: 671 889 521 678 411 147 274 1670 Fragments arranged by size: 1670 889 678 671 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 203 233 572 602 1633 1663 1895 1925 203 Size: 30 339 30 1031 30 232 30 3539 Fragments arranged by size: 3539 1031 339 232 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1933 1933 Size: 5261 BseYI C'CCAG_C Cuts at: 265 1871 2686 3444 4109 4517 265 Size: 1606 815 758 665 408 1009 Fragments arranged by size: 1606 1009 815 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 395 395 Size: 5261 BsiEI CG_ry'CG Cuts at: 104 2803 2849 4079 104 Size: 2699 46 1230 1286 Fragments arranged by size: 2699 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 127 501 2275 3053 3243 4103 127 Size: 374 1774 778 190 860 1285 Fragments arranged by size: 1774 1285 860 778 374 190 BsmI GAATG_Cn' Cuts at: 1230 1230 Size: 5261 BsmBI CGTCTCn'nnnn_ Cuts at: 592 592 Size: 5261 Bsp1286I G_dGCh'C Cuts at: 127 501 1872 2275 2886 2979 3053 3243 3305 Size: 374 1371 403 611 93 74 190 62 Cuts at: 3305 4103 127 Size: 798 1285 Fragments arranged by size: 1371 1285 798 611 403 374 190 93 74 62 BspEI T'CCGG_A Cuts at: 890 1561 890 Size: 671 4590 BspHI T'CATG_A Cuts at: 698 1731 3693 698 Size: 1033 1962 2266 Fragments arranged by size: 2266 1962 1033 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2311 2525 2827 3208 2311 Size: 214 302 381 4364 Fragments arranged by size: 4364 381 302 214 BsrBI CCG'CTC Cuts at: 50 3553 3607 5091 5256 50 Size: 3503 54 1484 165 55 Fragments arranged by size: 3503 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1781 3173 1781 Size: 1392 3869 BsrFI r'CCGG_y Cuts at: 3259 3440 3259 Size: 181 5080 BssSI C'ACGA_G Cuts at: 3532 4240 3532 Size: 708 4553 BstAPI GCAn_nnn'nTGC Cuts at: 2638 2638 Size: 5261 BstBI TT'CG_AA Cuts at: 3622 3622 Size: 5261 BstEII G'GTnAC_C Cuts at: 1629 2001 1629 Size: 372 4889 BstYI r'GATC_y Cuts at: 1 519 921 1150 2286 2753 2776 3111 3357 Size: 518 402 229 1136 467 23 335 246 Cuts at: 3357 3663 3675 3761 4813 1 Size: 306 12 86 1052 449 Fragments arranged by size: 1136 1052 518 467 449 402 335 306 246 229 86 23 12 Bsu36I CC'TnA_GG Cuts at: 805 805 Size: 5261 BtgI C'CryG_G Cuts at: 1804 1804 Size: 5261 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3211 3392 3555 3211 Size: 181 163 4917 Fragments arranged by size: 4917 181 163 BtsI GCAGTG_nn' Cuts at: 276 2504 276 Size: 2228 3033 DraI TTT'AAA Cuts at: 561 741 1347 2260 561 Size: 180 606 913 3562 Fragments arranged by size: 3562 913 606 180 DrdI GACnn_nn'nnGTC Cuts at: 2967 4311 2967 Size: 1344 3917 EaeI y'GGCC_r Cuts at: 532 2846 3020 3411 3438 4783 532 Size: 2314 174 391 27 1345 1010 Fragments arranged by size: 2314 1345 1010 391 174 27 EagI C'GGCC_G Cuts at: 2846 2846 Size: 5261 EarI CTCTTCn'nnn_ Cuts at: 506 832 1267 1902 3284 3494 506 Size: 326 435 635 1382 210 2273 Fragments arranged by size: 2273 1382 635 435 326 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3469 4211 4357 5071 3469 Size: 742 146 714 3659 Fragments arranged by size: 3659 742 714 146 EcoICRI GAG'CTC Cuts at: 499 2273 499 Size: 1774 3487 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 508 574 740 1666 3086 3518 3871 4533 508 Size: 66 166 926 1420 432 353 662 1236 Fragments arranged by size: 1420 1236 926 662 432 353 166 66 EcoO109I rG'GnC_Cy Cuts at: 294 1675 2400 294 Size: 1381 725 3155 Fragments arranged by size: 3155 1381 725 EcoRI G'AATT_C Cuts at: 1003 1058 2265 1003 Size: 55 1207 3999 Fragments arranged by size: 3999 1207 55 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1078 3280 1078 Size: 2202 3059 FspI TGC'GCA Cuts at: 3042 3042 Size: 5261 HaeII r_GCGC'y Cuts at: 634 2695 2943 4173 4944 634 Size: 2061 248 1230 771 951 Fragments arranged by size: 2061 1230 951 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 570 602 655 687 1302 1334 3103 3133 3135 Size: 32 53 32 615 32 1769 30 2 Cuts at: 3135 3165 570 Size: 30 2666 Fragments arranged by size: 2666 1769 615 53 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2300 2300 Size: 5261 HindIII A'AGCT_T Cuts at: 2026 2180 2026 Size: 154 5107 KasI G'GCGC_C Cuts at: 2691 2939 2691 Size: 248 5013 KpnI G_GTAC'C Cuts at: 2281 2281 Size: 5261 MluI A'CGCG_T Cuts at: 4694 4694 Size: 5261 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1050 1553 2225 4020 4204 1050 Size: 503 672 1795 184 2107 Fragments arranged by size: 2107 1795 672 503 184 MscI TGG'CCA Cuts at: 534 3022 534 Size: 2488 2773 MslI CAynn'nnrTG Cuts at: 239 731 3250 3377 239 Size: 492 2519 127 2123 Fragments arranged by size: 2519 2123 492 127 MspA1I CmG'CkG Cuts at: 336 1609 1875 2372 2686 3046 3830 4075 336 Size: 1273 266 497 314 360 784 245 1522 Fragments arranged by size: 1522 1273 784 497 360 314 266 245 NaeI GCC'GGC Cuts at: 3442 3442 Size: 5261 NarI GG'CG_CC Cuts at: 2692 2940 2692 Size: 248 5013 NdeI CA'TA_TG Cuts at: 2121 2121 Size: 5261 NgoMIV G'CCGG_C Cuts at: 3440 3440 Size: 5261 NsiI A_TGCA'T Cuts at: 738 1295 738 Size: 557 4704 NspI r_CATG'y Cuts at: 958 1437 1828 2314 2814 4417 958 Size: 479 391 486 500 1603 1802 Fragments arranged by size: 1802 1603 500 486 479 391 PasI CC'CwG_GG Cuts at: 1078 1078 Size: 5261 PciI A'CATG_T Cuts at: 954 1824 4413 954 Size: 870 2589 1802 Fragments arranged by size: 2589 1802 870 PflMI CCAn_nnn'nTGG Cuts at: 1084 1819 1084 Size: 735 4526 PfoI T'CCnGG_A Cuts at: 354 354 Size: 5261 PmeI GTTT'AAAC Cuts at: 2260 2260 Size: 5261 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 230 262 3707 3739 230 Size: 32 3445 32 1752 Fragments arranged by size: 3445 1752 32 32 PpuMI rG'GwC_Cy Cuts at: 294 294 Size: 5261 PshAI GACnn'nnGTC Cuts at: 1827 1827 Size: 5261 PspXI vC'TCGA_Gb Cuts at: 1541 1541 Size: 5261 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 450 482 450 Size: 32 5229 PstI C_TGCA'G Cuts at: 918 2308 2993 918 Size: 1390 685 3186 Fragments arranged by size: 3186 1390 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 5261 PvuII CAG'CTG Cuts at: 336 1609 1875 2686 3046 336 Size: 1273 266 811 360 2551 Fragments arranged by size: 2551 1273 811 360 266 RsrII CG'GwC_CG Cuts at: 3456 3456 Size: 5261 SacI G_AGCT'C Cuts at: 501 2275 501 Size: 1774 3487 SalI G'TCGA_C Cuts at: 2298 2298 Size: 5261 SapI GCTCTTCn'nnn_ Cuts at: 506 3284 3494 506 Size: 2778 210 2273 Fragments arranged by size: 2778 2273 210 SbfI CC_TGCA'GG Cuts at: 2308 2308 Size: 5261 ScaI AGT'ACT Cuts at: 529 4778 529 Size: 4249 1012 SfcI C'TryA_G Cuts at: 33 914 2043 2304 2989 3957 4148 33 Size: 881 1129 261 685 968 191 1146 Fragments arranged by size: 1146 1129 968 881 685 261 191 SfoI GGC'GCC Cuts at: 2693 2941 2693 Size: 248 5013 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5261 SmaI CCC'GGG Cuts at: 2283 2283 Size: 5261 SmlI C'TyrA_G Cuts at: 288 773 1541 2230 2416 3331 4045 4307 288 Size: 485 768 689 186 915 714 262 1242 Fragments arranged by size: 1242 915 768 714 689 485 262 186 SpeI A'CTAG_T Cuts at: 67 67 Size: 5261 SphI G_CATG'C Cuts at: 2314 2814 2314 Size: 500 4761 StyI C'CwwG_G Cuts at: 297 1450 2395 2749 297 Size: 1153 945 354 2809 Fragments arranged by size: 2809 1153 945 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1165 2844 2977 3436 3645 1165 Size: 1679 133 459 209 2781 Fragments arranged by size: 2781 1679 459 209 133 TatI w'GTAC_w Cuts at: 457 527 4776 457 Size: 70 4249 942 Fragments arranged by size: 4249 942 70 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 572 604 1895 1927 4778 4810 572 Size: 32 1291 32 2851 32 1023 Fragments arranged by size: 2851 1291 1023 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3058 3058 Size: 5261 XbaI T'CTAG_A Cuts at: 2292 2292 Size: 5261 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1641 2756 1641 Size: 1115 4146 XhoI C'TCGA_G Cuts at: 1541 1541 Size: 5261 XmaI C'CCGG_G Cuts at: 2281 2281 Size: 5261 XmnI GAAnn'nnTTC Cuts at: 761 761 Size: 5261 ZraI GAC'GTC Cuts at: 586 586 Size: 5261 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AgeI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BmgBI BmtI BsiWI BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI NruI PacI PmlI PsiI PspOMI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI