Restriction map for
pF1KB3092
(Circular) (Six-base) MAPSORT of: pF1KB3092.seq Check: 7977 from: 1 to: 5261
pF1KB3092 5261 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AatII G_ACGT'C
Cuts at: 588 588
Size: 5261
AccI GT'mk_AC
Cuts at: 595 2299 595
Size: 1704 3557
Acc65I G'GTAC_C
Cuts at: 2277 2277
Size: 5261
AclI AA'CG_TT
Cuts at: 5101 5101
Size: 5261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 740 3086 3518 3871 4533 508
Size: 232 2346 432 353 662 1236
Fragments arranged by size:
2346 1236 662 432 353 232
AflII C'TTAA_G
Cuts at: 288 288
Size: 5261
AflIII A'CryG_T
Cuts at: 954 1824 2613 4413 4694 954
Size: 870 789 1800 281 1521
Fragments arranged by size:
1800 1521 870 789 281
AhdI GACnn_n'nnGTC
Cuts at: 584 584
Size: 5261
AlwNI CAG_nnn'CTG
Cuts at: 1084 2566 4004 1084
Size: 1482 1438 2341
Fragments arranged by size:
2341 1482 1438
ApaLI G'TGCA_C
Cuts at: 4099 4099
Size: 5261
ApoI r'AATT_y
Cuts at: 1003 1024 1058 1156 1478 1664 2008 2265 4614
Size: 21 34 98 322 186 344 257 2349
Cuts at: 4614 4870 1003
Size: 256 1394
Fragments arranged by size:
2349 1394 344 322 257 256 186 98
34 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 5261
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5261
AvaI C'yCGr_G
Cuts at: 551 1103 1541 2281 551
Size: 552 438 740 3531
Fragments arranged by size:
3531 740 552 438
BamHI G'GATC_C
Cuts at: 1150 2286 1150
Size: 1136 4125
BanI G'GyrC_C
Cuts at: 2277 2691 2939 2974 2277
Size: 414 248 35 4564
Fragments arranged by size:
4564 414 248 35
BanII G_rGCy'C
Cuts at: 501 1872 2275 3305 501
Size: 1371 403 1030 2457
Fragments arranged by size:
2457 1371 1030 403
BbeI G_GCGC'C
Cuts at: 2695 2943 2695
Size: 248 5013
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 111 145 111
Size: 34 5227
BciVI GTATCCnnnnn_n'
Cuts at: 3159 4215 5261 3159
Size: 1056 1046 3159
Fragments arranged by size:
3159 1056 1046
BclI T'GATC_A
Cuts at: 330 1512 2781 330
Size: 1182 1269 2810
Fragments arranged by size:
2810 1269 1182
BfrBI ATG'CAT
Cuts at: 736 1293 736
Size: 557 4704
BglI GCCn_nnn'nGGC
Cuts at: 1809 1809
Size: 5261
BglII A'GATC_T
Cuts at: 519 2776 4813 519
Size: 2257 2037 967
Fragments arranged by size:
2257 2037 967
BlpI GC'TnA_GC
Cuts at: 2373 2373
Size: 5261
Bme1580I G_kGCm'C
Cuts at: 2886 2979 4103 2886
Size: 93 1124 4044
Fragments arranged by size:
4044 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2473 2554 2889 5033 2473
Size: 81 335 2144 2701
Fragments arranged by size:
2701 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3628 3628
Size: 5261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 1666 574
Size: 1092 4169
Bpu10I CC'TnA_GC
Cuts at: 251 251
Size: 5261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 2251 2437 3316 4030 4328 758
Size: 1493 186 879 714 298 1691
Fragments arranged by size:
1691 1493 879 714 298 186
BsaI GGTCTCn'nnnn_
Cuts at: 4965 4965
Size: 5261
BsaAI yAC'GTr
Cuts at: 2614 2614
Size: 5261
BsaBI GATnn'nnATC
Cuts at: 6 2775 4812 6
Size: 2769 2037 455
Fragments arranged by size:
2769 2037 455
BsaHI Gr'CG_yC
Cuts at: 585 2692 2940 3642 5138 585
Size: 2107 248 702 1496 708
Fragments arranged by size:
2107 1496 708 702 248
BsaWI w'CCGG_w
Cuts at: 890 1561 2450 2971 3649 4060 4207 4481 890
Size: 671 889 521 678 411 147 274 1670
Fragments arranged by size:
1670 889 678 671 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 203 233 572 602 1633 1663 1895 1925 203
Size: 30 339 30 1031 30 232 30 3539
Fragments arranged by size:
3539 1031 339 232 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1933 1933
Size: 5261
BseYI C'CCAG_C
Cuts at: 265 1871 2686 3444 4109 4517 265
Size: 1606 815 758 665 408 1009
Fragments arranged by size:
1606 1009 815 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 395
Size: 5261
BsiEI CG_ry'CG
Cuts at: 104 2803 2849 4079 104
Size: 2699 46 1230 1286
Fragments arranged by size:
2699 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 127 501 2275 3053 3243 4103 127
Size: 374 1774 778 190 860 1285
Fragments arranged by size:
1774 1285 860 778 374 190
BsmI GAATG_Cn'
Cuts at: 1230 1230
Size: 5261
BsmBI CGTCTCn'nnnn_
Cuts at: 592 592
Size: 5261
Bsp1286I G_dGCh'C
Cuts at: 127 501 1872 2275 2886 2979 3053 3243 3305
Size: 374 1371 403 611 93 74 190 62
Cuts at: 3305 4103 127
Size: 798 1285
Fragments arranged by size:
1371 1285 798 611 403 374 190 93
74 62
BspEI T'CCGG_A
Cuts at: 890 1561 890
Size: 671 4590
BspHI T'CATG_A
Cuts at: 698 1731 3693 698
Size: 1033 1962 2266
Fragments arranged by size:
2266 1962 1033
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2311 2525 2827 3208 2311
Size: 214 302 381 4364
Fragments arranged by size:
4364 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3553 3607 5091 5256 50
Size: 3503 54 1484 165 55
Fragments arranged by size:
3503 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1781 3173 1781
Size: 1392 3869
BsrFI r'CCGG_y
Cuts at: 3259 3440 3259
Size: 181 5080
BssSI C'ACGA_G
Cuts at: 3532 4240 3532
Size: 708 4553
BstAPI GCAn_nnn'nTGC
Cuts at: 2638 2638
Size: 5261
BstBI TT'CG_AA
Cuts at: 3622 3622
Size: 5261
BstEII G'GTnAC_C
Cuts at: 1629 2001 1629
Size: 372 4889
BstYI r'GATC_y
Cuts at: 1 519 921 1150 2286 2753 2776 3111 3357
Size: 518 402 229 1136 467 23 335 246
Cuts at: 3357 3663 3675 3761 4813 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1136 1052 518 467 449 402 335 306
246 229 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 805 805
Size: 5261
BtgI C'CryG_G
Cuts at: 1804 1804
Size: 5261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3211 3392 3555 3211
Size: 181 163 4917
Fragments arranged by size:
4917 181 163
BtsI GCAGTG_nn'
Cuts at: 276 2504 276
Size: 2228 3033
DraI TTT'AAA
Cuts at: 561 741 1347 2260 561
Size: 180 606 913 3562
Fragments arranged by size:
3562 913 606 180
DrdI GACnn_nn'nnGTC
Cuts at: 2967 4311 2967
Size: 1344 3917
EaeI y'GGCC_r
Cuts at: 532 2846 3020 3411 3438 4783 532
Size: 2314 174 391 27 1345 1010
Fragments arranged by size:
2314 1345 1010 391 174 27
EagI C'GGCC_G
Cuts at: 2846 2846
Size: 5261
EarI CTCTTCn'nnn_
Cuts at: 506 832 1267 1902 3284 3494 506
Size: 326 435 635 1382 210 2273
Fragments arranged by size:
2273 1382 635 435 326 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3469 4211 4357 5071 3469
Size: 742 146 714 3659
Fragments arranged by size:
3659 742 714 146
EcoICRI GAG'CTC
Cuts at: 499 2273 499
Size: 1774 3487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 574 740 1666 3086 3518 3871 4533 508
Size: 66 166 926 1420 432 353 662 1236
Fragments arranged by size:
1420 1236 926 662 432 353 166 66
EcoO109I rG'GnC_Cy
Cuts at: 294 1675 2400 294
Size: 1381 725 3155
Fragments arranged by size:
3155 1381 725
EcoRI G'AATT_C
Cuts at: 1003 1058 2265 1003
Size: 55 1207 3999
Fragments arranged by size:
3999 1207 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1078 3280 1078
Size: 2202 3059
FspI TGC'GCA
Cuts at: 3042 3042
Size: 5261
HaeII r_GCGC'y
Cuts at: 634 2695 2943 4173 4944 634
Size: 2061 248 1230 771 951
Fragments arranged by size:
2061 1230 951 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 570 602 655 687 1302 1334 3103 3133 3135
Size: 32 53 32 615 32 1769 30 2
Cuts at: 3135 3165 570
Size: 30 2666
Fragments arranged by size:
2666 1769 615 53 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2300 2300
Size: 5261
HindIII A'AGCT_T
Cuts at: 2026 2180 2026
Size: 154 5107
KasI G'GCGC_C
Cuts at: 2691 2939 2691
Size: 248 5013
KpnI G_GTAC'C
Cuts at: 2281 2281
Size: 5261
MluI A'CGCG_T
Cuts at: 4694 4694
Size: 5261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1050 1553 2225 4020 4204 1050
Size: 503 672 1795 184 2107
Fragments arranged by size:
2107 1795 672 503 184
MscI TGG'CCA
Cuts at: 534 3022 534
Size: 2488 2773
MslI CAynn'nnrTG
Cuts at: 239 731 3250 3377 239
Size: 492 2519 127 2123
Fragments arranged by size:
2519 2123 492 127
MspA1I CmG'CkG
Cuts at: 336 1609 1875 2372 2686 3046 3830 4075 336
Size: 1273 266 497 314 360 784 245 1522
Fragments arranged by size:
1522 1273 784 497 360 314 266 245
NaeI GCC'GGC
Cuts at: 3442 3442
Size: 5261
NarI GG'CG_CC
Cuts at: 2692 2940 2692
Size: 248 5013
NdeI CA'TA_TG
Cuts at: 2121 2121
Size: 5261
NgoMIV G'CCGG_C
Cuts at: 3440 3440
Size: 5261
NsiI A_TGCA'T
Cuts at: 738 1295 738
Size: 557 4704
NspI r_CATG'y
Cuts at: 958 1437 1828 2314 2814 4417 958
Size: 479 391 486 500 1603 1802
Fragments arranged by size:
1802 1603 500 486 479 391
PasI CC'CwG_GG
Cuts at: 1078 1078
Size: 5261
PciI A'CATG_T
Cuts at: 954 1824 4413 954
Size: 870 2589 1802
Fragments arranged by size:
2589 1802 870
PflMI CCAn_nnn'nTGG
Cuts at: 1084 1819 1084
Size: 735 4526
PfoI T'CCnGG_A
Cuts at: 354 354
Size: 5261
PmeI GTTT'AAAC
Cuts at: 2260 2260
Size: 5261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 230 262 3707 3739 230
Size: 32 3445 32 1752
Fragments arranged by size:
3445 1752 32 32
PpuMI rG'GwC_Cy
Cuts at: 294 294
Size: 5261
PshAI GACnn'nnGTC
Cuts at: 1827 1827
Size: 5261
PspXI vC'TCGA_Gb
Cuts at: 1541 1541
Size: 5261
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 450 482 450
Size: 32 5229
PstI C_TGCA'G
Cuts at: 918 2308 2993 918
Size: 1390 685 3186
Fragments arranged by size:
3186 1390 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5261
PvuII CAG'CTG
Cuts at: 336 1609 1875 2686 3046 336
Size: 1273 266 811 360 2551
Fragments arranged by size:
2551 1273 811 360 266
RsrII CG'GwC_CG
Cuts at: 3456 3456
Size: 5261
SacI G_AGCT'C
Cuts at: 501 2275 501
Size: 1774 3487
SalI G'TCGA_C
Cuts at: 2298 2298
Size: 5261
SapI GCTCTTCn'nnn_
Cuts at: 506 3284 3494 506
Size: 2778 210 2273
Fragments arranged by size:
2778 2273 210
SbfI CC_TGCA'GG
Cuts at: 2308 2308
Size: 5261
ScaI AGT'ACT
Cuts at: 529 4778 529
Size: 4249 1012
SfcI C'TryA_G
Cuts at: 33 914 2043 2304 2989 3957 4148 33
Size: 881 1129 261 685 968 191 1146
Fragments arranged by size:
1146 1129 968 881 685 261 191
SfoI GGC'GCC
Cuts at: 2693 2941 2693
Size: 248 5013
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5261
SmaI CCC'GGG
Cuts at: 2283 2283
Size: 5261
SmlI C'TyrA_G
Cuts at: 288 773 1541 2230 2416 3331 4045 4307 288
Size: 485 768 689 186 915 714 262 1242
Fragments arranged by size:
1242 915 768 714 689 485 262 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5261
SphI G_CATG'C
Cuts at: 2314 2814 2314
Size: 500 4761
StyI C'CwwG_G
Cuts at: 297 1450 2395 2749 297
Size: 1153 945 354 2809
Fragments arranged by size:
2809 1153 945 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1165 2844 2977 3436 3645 1165
Size: 1679 133 459 209 2781
Fragments arranged by size:
2781 1679 459 209 133
TatI w'GTAC_w
Cuts at: 457 527 4776 457
Size: 70 4249 942
Fragments arranged by size:
4249 942 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 572 604 1895 1927 4778 4810 572
Size: 32 1291 32 2851 32 1023
Fragments arranged by size:
2851 1291 1023 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3058 3058
Size: 5261
XbaI T'CTAG_A
Cuts at: 2292 2292
Size: 5261
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1641 2756 1641
Size: 1115 4146
XhoI C'TCGA_G
Cuts at: 1541 1541
Size: 5261
XmaI C'CCGG_G
Cuts at: 2281 2281
Size: 5261
XmnI GAAnn'nnTTC
Cuts at: 761 761
Size: 5261
ZraI GAC'GTC
Cuts at: 586 586
Size: 5261
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BmgBI BmtI BsiWI BsrGI
BssHII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NheI NotI NruI
PacI PmlI PsiI PspOMI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI