Restriction map for
pF1KB3095
(Circular) (Six-base) MAPSORT of: pF1KB3095.seq Check: 8191 from: 1 to: 4094
pF1KB3095 4094 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AatII G_ACGT'C
Cuts at: 1047 1047
Size: 4094
AccI GT'mk_AC
Cuts at: 310 1132 310
Size: 822 3272
Acc65I G'GTAC_C
Cuts at: 1110 1110
Size: 4094
AclI AA'CG_TT
Cuts at: 3934 3934
Size: 4094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 1919 2351 3098 3366 218
Size: 1701 432 747 268 946
Fragments arranged by size:
1701 946 747 432 268
AflII C'TTAA_G
Cuts at: 465 465
Size: 4094
AflIII A'CryG_T
Cuts at: 1446 2550 3527 1446
Size: 1104 977 2013
Fragments arranged by size:
2013 1104 977
AgeI A'CCGG_T
Cuts at: 2482 2482
Size: 4094
AhdI GACnn_n'nnGTC
Cuts at: 1048 1048
Size: 4094
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1056 1088 1056
Size: 32 4062
AlwNI CAG_nnn'CTG
Cuts at: 1399 2966 1399
Size: 1567 2527
ApaLI G'TGCA_C
Cuts at: 2864 2864
Size: 4094
ApoI r'AATT_y
Cuts at: 284 805 871 1035 1098 3447 3703 284
Size: 521 66 164 63 2349 256 675
Fragments arranged by size:
2349 675 521 256 164 66 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 4094
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
AvaI C'yCGr_G
Cuts at: 230 1114 230
Size: 884 3210
BamHI G'GATC_C
Cuts at: 1119 1119
Size: 4094
BanI G'GyrC_C
Cuts at: 1110 1524 1772 1807 1110
Size: 414 248 35 3397
Fragments arranged by size:
3397 414 248 35
BanII G_rGCy'C
Cuts at: 846 1108 2138 846
Size: 262 1030 2802
Fragments arranged by size:
2802 1030 262
BbeI G_GCGC'C
Cuts at: 1528 1776 1528
Size: 248 3846
BbsI GAAGACnn'nnnn_
Cuts at: 559 559
Size: 4094
BciVI GTATCCnnnnn_n'
Cuts at: 1992 2753 4094 1992
Size: 761 1341 1992
Fragments arranged by size:
1992 1341 761
BclI T'GATC_A
Cuts at: 1614 1614
Size: 4094
BglII A'GATC_T
Cuts at: 236 608 1609 3646 236
Size: 372 1001 2037 684
Fragments arranged by size:
2037 1001 684 372
BlpI GC'TnA_GC
Cuts at: 1206 1206
Size: 4094
Bme1580I G_kGCm'C
Cuts at: 1719 1812 2868 1719
Size: 93 1056 2945
Fragments arranged by size:
2945 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1306 1387 1722 3866 1306
Size: 81 335 2144 1534
Fragments arranged by size:
2144 1534 335 81
BmtI G_CTAG'C
Cuts at: 491 491
Size: 4094
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 4094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 175
Size: 4094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 2149 2641 2939 1270
Size: 879 492 298 2425
Fragments arranged by size:
2425 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 669 3798 669
Size: 3129 965
BsaAI yAC'GTr
Cuts at: 1447 1447
Size: 4094
BsaBI GATnn'nnATC
Cuts at: 6 1608 3645 6
Size: 1602 2037 455
Fragments arranged by size:
2037 1602 455
BsaHI Gr'CG_yC
Cuts at: 1044 1525 1773 2475 3971 1044
Size: 481 248 702 1496 1167
Fragments arranged by size:
1496 1167 702 481 248
BsaWI w'CCGG_w
Cuts at: 1283 1804 2482 2756 2903 3314 1283
Size: 521 678 274 147 411 2063
Fragments arranged by size:
2063 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 368 398 779 809 368
Size: 30 381 30 3653
Fragments arranged by size:
3653 381 30 30
BseYI C'CCAG_C
Cuts at: 749 1519 2277 2854 3350 749
Size: 770 758 577 496 1493
Fragments arranged by size:
1493 770 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 208
Size: 4094
BsiEI CG_ry'CG
Cuts at: 104 1636 1682 2890 104
Size: 1532 46 1208 1308
Fragments arranged by size:
1532 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 846 1108 1886 2076 2868 846
Size: 262 778 190 792 2072
Fragments arranged by size:
2072 792 778 262 190
BsmI GAATG_Cn'
Cuts at: 526 526
Size: 4094
Bsp1286I G_dGCh'C
Cuts at: 846 1108 1719 1812 1886 2076 2138 2868 846
Size: 262 611 93 74 190 62 730 2072
Fragments arranged by size:
2072 730 611 262 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3314 3314
Size: 4094
BspHI T'CATG_A
Cuts at: 819 3270 819
Size: 2451 1643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 650 1144 1358 1660 2041 650
Size: 494 214 302 381 2703
Fragments arranged by size:
2703 494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2386 2440 3924 4089 50
Size: 2336 54 1484 165 55
Fragments arranged by size:
2336 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2006 2006
Size: 4094
BsrFI r'CCGG_y
Cuts at: 2092 2273 2482 2092
Size: 181 209 3704
Fragments arranged by size:
3704 209 181
BssSI C'ACGA_G
Cuts at: 2365 2723 2365
Size: 358 3736
BstAPI GCAn_nnn'nTGC
Cuts at: 1471 1471
Size: 4094
BstBI TT'CG_AA
Cuts at: 2455 2455
Size: 4094
BstEII G'GTnAC_C
Cuts at: 894 894
Size: 4094
BstXI CCAn_nnnn'nTGG
Cuts at: 1027 1027
Size: 4094
BstYI r'GATC_y
Cuts at: 1 236 608 1119 1586 1609 1944 2190 3202
Size: 235 372 511 467 23 335 246 1012
Cuts at: 3202 3288 3300 3646 1
Size: 86 12 346 449
Fragments arranged by size:
1012 511 467 449 372 346 335 246
235 86 23 12
BstZ17I GTA'TAC
Cuts at: 311 311
Size: 4094
BtgI C'CryG_G
Cuts at: 107 107
Size: 4094
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2044 2225 2388 2044
Size: 181 163 3750
Fragments arranged by size:
3750 181 163
BtsI GCAGTG_nn'
Cuts at: 1337 1337
Size: 4094
DraI TTT'AAA
Cuts at: 1093 1093
Size: 4094
DrdI GACnn_nn'nnGTC
Cuts at: 1800 2658 1800
Size: 858 3236
EaeI y'GGCC_r
Cuts at: 1679 1853 2244 2271 3616 1679
Size: 174 391 27 1345 2157
Fragments arranged by size:
2157 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1679 1679
Size: 4094
EarI CTCTTCn'nnn_
Cuts at: 2117 2327 2117
Size: 210 3884
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1064 2302 2612 2758 3904 127
Size: 937 1238 310 146 1146 317
Fragments arranged by size:
1238 1146 937 317 310 146
EcoICRI GAG'CTC
Cuts at: 844 1106 844
Size: 262 3832
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 218 1919 2351 3098 3366 175
Size: 43 1701 432 747 268 903
Fragments arranged by size:
1701 903 747 432 268 43
EcoNI CCTnn'n_nnAGG
Cuts at: 695 695
Size: 4094
EcoO109I rG'GnC_Cy
Cuts at: 1233 1233
Size: 4094
EcoRI G'AATT_C
Cuts at: 1035 1098 1035
Size: 63 4031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 4094
FspI TGC'GCA
Cuts at: 1875 1875
Size: 4094
HaeII r_GCGC'y
Cuts at: 1528 1776 2798 3777 1528
Size: 248 1022 979 1845
Fragments arranged by size:
1845 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 631 663 1034 1066 1936 1966 1968 1998 631
Size: 32 371 32 870 30 2 30 2727
Fragments arranged by size:
2727 870 371 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1133 1133
Size: 4094
KasI G'GCGC_C
Cuts at: 1524 1772 1524
Size: 248 3846
KpnI G_GTAC'C
Cuts at: 1114 1114
Size: 4094
MluI A'CGCG_T
Cuts at: 3527 3527
Size: 4094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2765 2949 2765
Size: 184 3910
MscI TGG'CCA
Cuts at: 1855 1855
Size: 4094
MslI CAynn'nnrTG
Cuts at: 482 2083 2210 482
Size: 1601 127 2366
Fragments arranged by size:
2366 1601 127
MspA1I CmG'CkG
Cuts at: 279 1205 1519 1879 2892 3137 279
Size: 926 314 360 1013 245 1236
Fragments arranged by size:
1236 1013 926 360 314 245
NaeI GCC'GGC
Cuts at: 2275 2275
Size: 4094
NarI GG'CG_CC
Cuts at: 1525 1773 1525
Size: 248 3846
NcoI C'CATG_G
Cuts at: 107 107
Size: 4094
NgoMIV G'CCGG_C
Cuts at: 2273 2273
Size: 4094
NheI G'CTAG_C
Cuts at: 487 487
Size: 4094
NspI r_CATG'y
Cuts at: 1147 1647 2554 1147
Size: 500 907 2687
Fragments arranged by size:
2687 907 500
PciI A'CATG_T
Cuts at: 2550 2550
Size: 4094
PfoI T'CCnGG_A
Cuts at: 174 174
Size: 4094
PmeI GTTT'AAAC
Cuts at: 1093 1093
Size: 4094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 4062
PsiI TTA'TAA
Cuts at: 434 434
Size: 4094
PstI C_TGCA'G
Cuts at: 1141 1826 1141
Size: 685 3409
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4094
PvuII CAG'CTG
Cuts at: 1519 1879 1519
Size: 360 3734
RsrII CG'GwC_CG
Cuts at: 2289 2289
Size: 4094
SacI G_AGCT'C
Cuts at: 846 1108 846
Size: 262 3832
SalI G'TCGA_C
Cuts at: 1131 1131
Size: 4094
SapI GCTCTTCn'nnn_
Cuts at: 2117 2327 2117
Size: 210 3884
SbfI CC_TGCA'GG
Cuts at: 1141 1141
Size: 4094
ScaI AGT'ACT
Cuts at: 3611 3611
Size: 4094
SfcI C'TryA_G
Cuts at: 33 584 1137 1822 2815 3006 33
Size: 551 553 685 993 191 1121
Fragments arranged by size:
1121 993 685 553 551 191
SfoI GGC'GCC
Cuts at: 1526 1774 1526
Size: 248 3846
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
SmaI CCC'GGG
Cuts at: 1116 1116
Size: 4094
SmlI C'TyrA_G
Cuts at: 465 1249 2164 2656 2918 465
Size: 784 915 492 262 1641
Fragments arranged by size:
1641 915 784 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4094
SphI G_CATG'C
Cuts at: 1147 1647 1147
Size: 500 3594
SspI AAT'ATT
Cuts at: 540 540
Size: 4094
StyI C'CwwG_G
Cuts at: 107 1228 1582 107
Size: 1121 354 2619
Fragments arranged by size:
2619 1121 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1677 1810 2269 2478 1677
Size: 133 459 209 3293
Fragments arranged by size:
3293 459 209 133
TatI w'GTAC_w
Cuts at: 3609 3609
Size: 4094
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 170 202 368 400 3611 3643 170
Size: 32 166 32 3211 32 621
Fragments arranged by size:
3211 621 166 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1891 1891
Size: 4094
XbaI T'CTAG_A
Cuts at: 1125 1125
Size: 4094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1589 1589
Size: 4094
XmaI C'CCGG_G
Cuts at: 1114 1114
Size: 4094
XmnI GAAnn'nnTTC
Cuts at: 556 610 556
Size: 54 4040
ZraI GAC'GTC
Cuts at: 1045 1045
Size: 4094
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PfoI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AleI AlfI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI Bpu10I BseRI BsiWI
BsmBI BsrGI BssHII Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI