Restriction map for
pF1KB3100
(Circular) (Six-base) MAPSORT of: pF1KB3100.seq Check: 8799 from: 1 to: 5681
pF1KB3100 5681 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2460 2460
Size: 5681
AccI GT'mk_AC
Cuts at: 928 2719 928
Size: 1791 3890
Acc65I G'GTAC_C
Cuts at: 1468 2697 1468
Size: 1229 4452
AclI AA'CG_TT
Cuts at: 5521 5521
Size: 5681
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 596 725 3506 3938 4291 4953 145
Size: 451 129 2781 432 353 662 873
Fragments arranged by size:
2781 873 662 451 432 353 129
AfeI AGC'GCT
Cuts at: 1930 1990 1930
Size: 60 5621
AflIII A'CryG_T
Cuts at: 3033 4833 5114 3033
Size: 1800 281 3600
Fragments arranged by size:
3600 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 937 2986 4424 937
Size: 2049 1438 2194
Fragments arranged by size:
2194 2049 1438
ApaLI G'TGCA_C
Cuts at: 4519 4519
Size: 5681
ApoI r'AATT_y
Cuts at: 2685 5034 5290 2685
Size: 2349 256 3076
Fragments arranged by size:
3076 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5681
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5681
AvaI C'yCGr_G
Cuts at: 173 277 349 384 782 1290 2701 173
Size: 104 72 35 398 508 1411 3153
Fragments arranged by size:
3153 1411 508 398 104 72 35
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 434 467 722 755 434
Size: 33 255 33 5360
Fragments arranged by size:
5360 255 33 33
BamHI G'GATC_C
Cuts at: 2706 2706
Size: 5681
BanI G'GyrC_C
Cuts at: 1339 1468 2697 3111 3359 3394 1339
Size: 129 1229 414 248 35 3626
Fragments arranged by size:
3626 1229 414 248 129 35
BanII G_rGCy'C
Cuts at: 251 316 374 846 1274 2181 2695 3725 251
Size: 65 58 472 428 907 514 1030 2207
Fragments arranged by size:
2207 1030 907 514 472 428 65 58
BbeI G_GCGC'C
Cuts at: 3115 3363 3115
Size: 248 5433
BbsI GAAGACnn'nnnn_
Cuts at: 538 538
Size: 5681
BbvCI CC'TCA_GC
Cuts at: 692 2534 692
Size: 1842 3839
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 499 533 499
Size: 34 5647
BciVI GTATCCnnnnn_n'
Cuts at: 1342 3579 4635 5681 1342
Size: 2237 1056 1046 1342
Fragments arranged by size:
2237 1342 1056 1046
BclI T'GATC_A
Cuts at: 3201 3201
Size: 5681
BglI GCCn_nnn'nGGC
Cuts at: 351 600 351
Size: 249 5432
BglII A'GATC_T
Cuts at: 3196 5233 3196
Size: 2037 3644
BlpI GC'TnA_GC
Cuts at: 197 2793 197
Size: 2596 3085
Bme1580I G_kGCm'C
Cuts at: 284 1342 3306 3399 4523 284
Size: 1058 1964 93 1124 1442
Fragments arranged by size:
1964 1442 1124 1058 93
BmgBI CAC'GTC
Cuts at: 1128 1128
Size: 5681
BmrI ACTGGGnnnn_n'
Cuts at: 1037 1557 2893 2974 3309 5453 1037
Size: 520 1336 81 335 2144 1265
Fragments arranged by size:
2144 1336 1265 520 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 330 924 1023 4048 330
Size: 594 99 3025 1963
Fragments arranged by size:
3025 1963 594 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 2033 343
Size: 1690 3991
Bpu10I CC'TnA_GC
Cuts at: 692 2534 692
Size: 1842 3839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 574 2304 2857 3736 4450 4748 166
Size: 408 1730 553 879 714 298 1099
Fragments arranged by size:
1730 1099 879 714 553 408 298
BsaI GGTCTCn'nnnn_
Cuts at: 295 480 1656 5385 295
Size: 185 1176 3729 591
Fragments arranged by size:
3729 1176 591 185
BsaAI yAC'GTr
Cuts at: 948 3034 948
Size: 2086 3595
BsaBI GATnn'nnATC
Cuts at: 6 892 3195 5232 6
Size: 886 2303 2037 455
Fragments arranged by size:
2303 2037 886 455
BsaHI Gr'CG_yC
Cuts at: 254 3112 3360 4062 5558 254
Size: 2858 248 702 1496 377
Fragments arranged by size:
2858 1496 702 377 248
BsaWI w'CCGG_w
Cuts at: 2870 3391 4069 4480 4627 4901 2870
Size: 521 678 411 147 274 3650
Fragments arranged by size:
3650 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2134 2164 2134
Size: 30 5651
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 241 307 498 680 2075 2090 160
Size: 81 66 191 182 1395 15 3751
Fragments arranged by size:
3751 1395 191 182 81 66 15
BseYI C'CCAG_C
Cuts at: 986 1535 1909 2120 2183 2231 2251 2539 2567
Size: 549 374 211 63 48 20 288 28
Cuts at: 2567 3106 3864 4529 4937 986
Size: 539 758 665 408 1730
Fragments arranged by size:
1730 758 665 549 539 408 374 288
211 63 48 28 20
BsiEI CG_ry'CG
Cuts at: 104 3223 3269 4499 104
Size: 3119 46 1230 1286
Fragments arranged by size:
3119 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 251 555 665 2695 3473 3663 4523 251
Size: 304 110 2030 778 190 860 1409
Fragments arranged by size:
2030 1409 860 778 304 190 110
BsmBI CGTCTCn'nnnn_
Cuts at: 1644 1644
Size: 5681
Bsp1286I G_dGCh'C
Cuts at: 251 284 316 374 555 665 846 1274 1342
Size: 33 32 58 181 110 181 428 68
Cuts at: 1342 2181 2695 3306 3399 3473 3663 3725 4523
Size: 839 514 611 93 74 190 62 798
Cuts at: 4523 251
Size: 1409
Fragments arranged by size:
1409 839 798 611 514 428 190 181
181 110 93 74 68 62 58 33
32
BspHI T'CATG_A
Cuts at: 4113 4113
Size: 5681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2460 2731 2945 3247 3628 2460
Size: 271 214 302 381 4513
Fragments arranged by size:
4513 381 302 271 214
BsrBI CCG'CTC
Cuts at: 50 201 243 686 807 3973 4027 5511 5676
Size: 151 42 443 121 3166 54 1484 165
Cuts at: 5676 50
Size: 55
Fragments arranged by size:
3166 1484 443 165 151 121 55 54
42
BsrDI GCAATG_nn'
Cuts at: 1166 3593 1166
Size: 2427 3254
BsrFI r'CCGG_y
Cuts at: 1049 1511 3679 3860 1049
Size: 462 2168 181 2870
Fragments arranged by size:
2870 2168 462 181
BssSI C'ACGA_G
Cuts at: 3952 4660 3952
Size: 708 4973
BstAPI GCAn_nnn'nTGC
Cuts at: 3058 3058
Size: 5681
BstBI TT'CG_AA
Cuts at: 4042 4042
Size: 5681
BstYI r'GATC_y
Cuts at: 1 2706 3173 3196 3531 3777 4083 4095 4181
Size: 2705 467 23 335 246 306 12 86
Cuts at: 4181 5233 1
Size: 1052 449
Fragments arranged by size:
2705 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 159 407 1965 2022 107
Size: 52 248 1558 57 3766
Fragments arranged by size:
3766 1558 248 57 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3631 3812 3975 3631
Size: 181 163 5337
Fragments arranged by size:
5337 181 163
BtsI GCAGTG_nn'
Cuts at: 1247 2578 2924 1247
Size: 1331 346 4004
Fragments arranged by size:
4004 1331 346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1926 1961 1926
Size: 35 5646
DraI TTT'AAA
Cuts at: 2680 2680
Size: 5681
DraIII CAC_nnn'GTG
Cuts at: 1565 2659 1565
Size: 1094 4587
DrdI GACnn_nn'nnGTC
Cuts at: 3387 4731 3387
Size: 1344 4337
EaeI y'GGCC_r
Cuts at: 1530 2605 3266 3440 3831 3858 5203 1530
Size: 1075 661 174 391 27 1345 2008
Fragments arranged by size:
2008 1345 1075 661 391 174 27
EagI C'GGCC_G
Cuts at: 3266 3266
Size: 5681
EarI CTCTTCn'nnn_
Cuts at: 701 992 3704 3914 701
Size: 291 2712 210 2468
Fragments arranged by size:
2712 2468 291 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2201 3889 4631 4777 5491 2201
Size: 1688 742 146 714 2391
Fragments arranged by size:
2391 1688 742 714 146
EcoICRI GAG'CTC
Cuts at: 249 2693 249
Size: 2444 3237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 343 596 725 2033 3506 3938 4291 4953
Size: 198 253 129 1308 1473 432 353 662
Cuts at: 4953 145
Size: 873
Fragments arranged by size:
1473 1308 873 662 432 353 253 198
129
EcoNI CCTnn'n_nnAGG
Cuts at: 1831 1831
Size: 5681
EcoO109I rG'GnC_Cy
Cuts at: 1206 1716 2005 2820 1206
Size: 510 289 815 4067
Fragments arranged by size:
4067 815 510 289
EcoRI G'AATT_C
Cuts at: 2685 2685
Size: 5681
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1209 3700 1209
Size: 2491 3190
FspI TGC'GCA
Cuts at: 1239 3462 1239
Size: 2223 3458
HaeII r_GCGC'y
Cuts at: 159 1932 1992 2327 3115 3363 4593 5364 159
Size: 1773 60 335 788 248 1230 771 476
Fragments arranged by size:
1773 1230 788 771 476 335 248 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2517 2549 3523 3553 3555 3585 2517
Size: 32 974 30 2 30 4613
Fragments arranged by size:
4613 974 32 30 30 2
HincII GTy'rAC
Cuts at: 2720 2720
Size: 5681
HindIII A'AGCT_T
Cuts at: 578 578
Size: 5681
KasI G'GCGC_C
Cuts at: 3111 3359 3111
Size: 248 5433
KpnI G_GTAC'C
Cuts at: 1472 2701 1472
Size: 1229 4452
MfeI C'AATT_G
Cuts at: 812 812
Size: 5681
MluI A'CGCG_T
Cuts at: 5114 5114
Size: 5681
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 1326 1463 2177 4440 4624 197
Size: 1129 137 714 2263 184 1254
Fragments arranged by size:
2263 1254 1129 714 184 137
MscI TGG'CCA
Cuts at: 3442 3442
Size: 5681
MslI CAynn'nnrTG
Cuts at: 112 831 3670 3797 112
Size: 719 2839 127 1996
Fragments arranged by size:
2839 1996 719 127
MspA1I CmG'CkG
Cuts at: 161 1518 1535 1857 1967 2054 2093 2120 2792
Size: 1357 17 322 110 87 39 27 672
Cuts at: 2792 3106 3466 4250 4495 161
Size: 314 360 784 245 1347
Fragments arranged by size:
1357 1347 784 672 360 322 314 245
110 87 39 27 17
NaeI GCC'GGC
Cuts at: 1513 3862 1513
Size: 2349 3332
NarI GG'CG_CC
Cuts at: 3112 3360 3112
Size: 248 5433
NcoI C'CATG_G
Cuts at: 107 407 107
Size: 300 5381
NgoMIV G'CCGG_C
Cuts at: 1511 3860 1511
Size: 2349 3332
NruI TCG'CGA
Cuts at: 143 143
Size: 5681
NspI r_CATG'y
Cuts at: 1432 2734 3234 4837 1432
Size: 1302 500 1603 2276
Fragments arranged by size:
2276 1603 1302 500
PasI CC'CwG_GG
Cuts at: 2278 2278
Size: 5681
PciI A'CATG_T
Cuts at: 4833 4833
Size: 5681
PflMI CCAn_nnn'nTGG
Cuts at: 1816 1816
Size: 5681
PfoI T'CCnGG_A
Cuts at: 1826 2644 1826
Size: 818 4863
PmeI GTTT'AAAC
Cuts at: 2680 2680
Size: 5681
PmlI CAC'GTG
Cuts at: 948 948
Size: 5681
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1825 1857 4127 4159 1825
Size: 32 2270 32 3347
Fragments arranged by size:
3347 2270 32 32
PpuMI rG'GwC_Cy
Cuts at: 1716 2005 1716
Size: 289 5392
PspXI vC'TCGA_Gb
Cuts at: 173 173
Size: 5681
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 421 453 2614 2646 421
Size: 32 2161 32 3456
Fragments arranged by size:
3456 2161 32 32
PstI C_TGCA'G
Cuts at: 155 209 359 982 1856 2728 3413 155
Size: 54 150 623 874 872 685 2423
Fragments arranged by size:
2423 874 872 685 623 150 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5681
PvuII CAG'CTG
Cuts at: 3106 3466 3106
Size: 360 5321
RsrII CG'GwC_CG
Cuts at: 3876 3876
Size: 5681
SacI G_AGCT'C
Cuts at: 251 2695 251
Size: 2444 3237
SacII CC_GC'GG
Cuts at: 162 1968 162
Size: 1806 3875
SalI G'TCGA_C
Cuts at: 2718 2718
Size: 5681
SapI GCTCTTCn'nnn_
Cuts at: 992 3704 3914 992
Size: 2712 210 2759
Fragments arranged by size:
2759 2712 210
SbfI CC_TGCA'GG
Cuts at: 2728 2728
Size: 5681
ScaI AGT'ACT
Cuts at: 2584 5198 2584
Size: 2614 3067
SfcI C'TryA_G
Cuts at: 33 151 205 355 929 978 1852 2106 2256
Size: 118 54 150 574 49 874 254 150
Cuts at: 2256 2355 2724 3409 4377 4568 33
Size: 99 369 685 968 191 1146
Fragments arranged by size:
1146 968 874 685 574 369 254 191
150 150 118 99 54 49
SfoI GGC'GCC
Cuts at: 3113 3361 3113
Size: 248 5433
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5681
SmaI CCC'GGG
Cuts at: 279 351 1292 2703 279
Size: 72 941 1411 3257
Fragments arranged by size:
3257 1411 941 72
SmlI C'TyrA_G
Cuts at: 173 181 589 2319 2836 3751 4465 4727 173
Size: 8 408 1730 517 915 714 262 1127
Fragments arranged by size:
1730 1127 915 714 517 408 262 8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5681
SphI G_CATG'C
Cuts at: 2734 3234 2734
Size: 500 5181
SrfI GCCC'GGGC
Cuts at: 351 351
Size: 5681
SspI AAT'ATT
Cuts at: 417 417
Size: 5681
StuI AGG'CCT
Cuts at: 179 514 627 179
Size: 335 113 5233
Fragments arranged by size:
5233 335 113
StyI C'CwwG_G
Cuts at: 107 407 1193 2815 3169 107
Size: 300 786 1622 354 2619
Fragments arranged by size:
2619 1622 786 354 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 319 570 1000 1576 1742 2265 3264 3397 3856
Size: 251 430 576 166 523 999 133 459
Cuts at: 3856 4065 319
Size: 209 1935
Fragments arranged by size:
1935 999 576 523 459 430 251 209
166 133
TatI w'GTAC_w
Cuts at: 1454 1886 2582 2668 5196 1454
Size: 432 696 86 2528 1939
Fragments arranged by size:
2528 1939 696 432 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1112 1144 5198 5230 1112
Size: 32 4054 32 1563
Fragments arranged by size:
4054 1563 32 32
Tth111I GACn'n_nGTC
Cuts at: 3478 3478
Size: 5681
XbaI T'CTAG_A
Cuts at: 2712 2712
Size: 5681
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1479 3176 1479
Size: 1697 3984
XhoI C'TCGA_G
Cuts at: 173 173
Size: 5681
XmaI C'CCGG_G
Cuts at: 277 349 1290 2701 277
Size: 72 941 1411 3257
Fragments arranged by size:
3257 1411 941 72
XmnI GAAnn'nnTTC
Cuts at: 433 433
Size: 5681
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BfrBI BmtI BsgI BsiWI BsmI BspEI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI EcoRV
FseI FspAI HpaI NdeI NheI NotI NsiI PacI
PshAI PsiI PspOMI SanDI SexAI SfiI SgrAI SnaBI
SwaI ZraI