Restriction map for
pF1KB3103
(Circular) (Six-base) MAPSORT of: pF1KB3103.seq Check: 4749 from: 1 to: 4763
pF1KB3103 4763 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 438 438
Size: 4763
AccI GT'mk_AC
Cuts at: 614 1801 614
Size: 1187 3576
Acc65I G'GTAC_C
Cuts at: 1779 1779
Size: 4763
AclI AA'CG_TT
Cuts at: 4603 4603
Size: 4763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 2588 3020 3373 4035 104
Size: 2484 432 353 662 832
Fragments arranged by size:
2484 832 662 432 353
AflII C'TTAA_G
Cuts at: 313 313
Size: 4763
AflIII A'CryG_T
Cuts at: 2115 3915 4196 2115
Size: 1800 281 2682
Fragments arranged by size:
2682 1800 281
AleI CACnn'nnGTG
Cuts at: 1093 1093
Size: 4763
AlwNI CAG_nnn'CTG
Cuts at: 1123 1491 1510 2068 3506 1123
Size: 368 19 558 1438 2380
Fragments arranged by size:
2380 1438 558 368 19
ApaLI G'TGCA_C
Cuts at: 3601 3601
Size: 4763
ApoI r'AATT_y
Cuts at: 367 811 922 1767 4116 4372 367
Size: 444 111 845 2349 256 758
Fragments arranged by size:
2349 845 758 444 256 111
AseI AT'TA_AT
Cuts at: 20 20
Size: 4763
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
AvaI C'yCGr_G
Cuts at: 1356 1533 1783 1356
Size: 177 250 4336
Fragments arranged by size:
4336 250 177
BamHI G'GATC_C
Cuts at: 1788 1788
Size: 4763
BanI G'GyrC_C
Cuts at: 226 251 1779 2193 2441 2476 226
Size: 25 1528 414 248 35 2513
Fragments arranged by size:
2513 1528 414 248 35 25
BanII G_rGCy'C
Cuts at: 247 1230 1363 1439 1727 1777 2807 247
Size: 983 133 76 288 50 1030 2203
Fragments arranged by size:
2203 1030 983 288 133 76 50
BbeI G_GCGC'C
Cuts at: 2197 2445 2197
Size: 248 4515
BbsI GAAGACnn'nnnn_
Cuts at: 544 1120 544
Size: 576 4187
BbvCI CC'TCA_GC
Cuts at: 280 1331 280
Size: 1051 3712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1033 1067 1033
Size: 34 4729
BciVI GTATCCnnnnn_n'
Cuts at: 2661 3717 4763 2661
Size: 1056 1046 2661
Fragments arranged by size:
2661 1056 1046
BclI T'GATC_A
Cuts at: 504 2283 504
Size: 1779 2984
BglI GCCn_nnn'nGGC
Cuts at: 1487 1507 1487
Size: 20 4743
BglII A'GATC_T
Cuts at: 1637 2278 4315 1637
Size: 641 2037 2085
Fragments arranged by size:
2085 2037 641
BlpI GC'TnA_GC
Cuts at: 1399 1875 1399
Size: 476 4287
Bme1580I G_kGCm'C
Cuts at: 254 849 2388 2481 3605 254
Size: 595 1539 93 1124 1412
Fragments arranged by size:
1539 1412 1124 595 93
BmrI ACTGGGnnnn_n'
Cuts at: 180 1975 2056 2391 4535 180
Size: 1795 81 335 2144 408
Fragments arranged by size:
2144 1795 408 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 4763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 1300 555
Size: 745 4018
Bpu10I CC'TnA_GC
Cuts at: 280 1181 1331 280
Size: 901 150 3712
Fragments arranged by size:
3712 901 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1939 2818 3532 3830 1939
Size: 879 714 298 2872
Fragments arranged by size:
2872 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 169 4467 169
Size: 4298 465
BsaAI yAC'GTr
Cuts at: 2116 2116
Size: 4763
BsaBI GATnn'nnATC
Cuts at: 6 2277 4314 6
Size: 2271 2037 455
Fragments arranged by size:
2271 2037 455
BsaHI Gr'CG_yC
Cuts at: 2194 2442 3144 4640 2194
Size: 248 702 1496 2317
Fragments arranged by size:
2317 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1952 2473 3151 3562 3709 3983 1952
Size: 521 678 411 147 274 2732
Fragments arranged by size:
2732 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1426 1456 1426
Size: 30 4733
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1297 1357 1297
Size: 60 4703
BseYI C'CCAG_C
Cuts at: 854 1001 1229 1731 2188 2946 3611 4019 854
Size: 147 228 502 457 758 665 408 1598
Fragments arranged by size:
1598 758 665 502 457 408 228 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 619 841 1063 259
Size: 360 222 222 3959
Fragments arranged by size:
3959 360 222 222
BsiEI CG_ry'CG
Cuts at: 104 609 2305 2351 3581 104
Size: 505 1696 46 1230 1286
Fragments arranged by size:
1696 1286 1230 505 46
BsiHKAI G_wGCw'C
Cuts at: 1777 2555 2745 3605 1777
Size: 778 190 860 2935
Fragments arranged by size:
2935 860 778 190
BsmI GAATG_Cn'
Cuts at: 1071 1071
Size: 4763
Bsp1286I G_dGCh'C
Cuts at: 247 254 849 1230 1363 1439 1727 1777 2388
Size: 7 595 381 133 76 288 50 611
Cuts at: 2388 2481 2555 2745 2807 3605 247
Size: 93 74 190 62 798 1405
Fragments arranged by size:
1405 798 611 595 381 288 190 133
93 76 74 62 50 7
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 438 1746 1813 2027 2329 2710 438
Size: 1308 67 214 302 381 2491
Fragments arranged by size:
2491 1308 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 1248 3055 3109 4593 4758 50
Size: 1198 1807 54 1484 165 55
Fragments arranged by size:
1807 1484 1198 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2675 2675
Size: 4763
BsrFI r'CCGG_y
Cuts at: 1715 2761 2942 1715
Size: 1046 181 3536
Fragments arranged by size:
3536 1046 181
BsrGI T'GTAC_A
Cuts at: 1442 1442
Size: 4763
BssSI C'ACGA_G
Cuts at: 661 772 994 3034 3742 661
Size: 111 222 2040 708 1682
Fragments arranged by size:
2040 1682 708 222 111
BstAPI GCAn_nnn'nTGC
Cuts at: 2140 2140
Size: 4763
BstBI TT'CG_AA
Cuts at: 3124 3124
Size: 4763
BstXI CCAn_nnnn'nTGG
Cuts at: 358 1743 358
Size: 1385 3378
BstYI r'GATC_y
Cuts at: 1 1637 1788 2255 2278 2613 2859 3165 3177
Size: 1636 151 467 23 335 246 306 12
Cuts at: 3177 3263 4315 1
Size: 86 1052 449
Fragments arranged by size:
1636 1052 467 449 335 306 246 151
86 23 12
BtgI C'CryG_G
Cuts at: 351 462 351
Size: 111 4652
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 126 1643 2713 2894 3057 126
Size: 1517 1070 181 163 1832
Fragments arranged by size:
1832 1517 1070 181 163
BtsI GCAGTG_nn'
Cuts at: 438 2006 438
Size: 1568 3195
DraI TTT'AAA
Cuts at: 1762 1762
Size: 4763
DraIII CAC_nnn'GTG
Cuts at: 135 135
Size: 4763
DrdI GACnn_nn'nnGTC
Cuts at: 2469 3813 2469
Size: 1344 3419
EaeI y'GGCC_r
Cuts at: 408 986 1238 2348 2522 2913 2940 4285 408
Size: 578 252 1110 174 391 27 1345 886
Fragments arranged by size:
1345 1110 886 578 391 252 174 27
EagI C'GGCC_G
Cuts at: 2348 2348
Size: 4763
EarI CTCTTCn'nnn_
Cuts at: 1256 1732 2786 2996 1256
Size: 476 1054 210 3023
Fragments arranged by size:
3023 1054 476 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1221 1228 1234 2971 3713 3859 4573 1221
Size: 7 6 1737 742 146 714 1411
Fragments arranged by size:
1737 1411 742 714 146 7 6
EcoICRI GAG'CTC
Cuts at: 1775 1775
Size: 4763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 555 1300 2588 3020 3373 4035 104
Size: 451 745 1288 432 353 662 832
Fragments arranged by size:
1288 832 745 662 451 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1180 1180
Size: 4763
EcoO109I rG'GnC_Cy
Cuts at: 1411 1488 1530 1902 1411
Size: 77 42 372 4272
Fragments arranged by size:
4272 372 77 42
EcoRI G'AATT_C
Cuts at: 1767 1767
Size: 4763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4763
FspI TGC'GCA
Cuts at: 2544 2544
Size: 4763
HaeII r_GCGC'y
Cuts at: 737 2197 2445 3675 4446 737
Size: 1460 248 1230 771 1054
Fragments arranged by size:
1460 1230 1054 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1624 1656 2605 2635 2637 2667 1624
Size: 32 949 30 2 30 3720
Fragments arranged by size:
3720 949 32 30 30 2
HincII GTy'rAC
Cuts at: 1073 1802 1073
Size: 729 4034
HindIII A'AGCT_T
Cuts at: 116 344 116
Size: 228 4535
KasI G'GCGC_C
Cuts at: 2193 2441 2193
Size: 248 4515
KpnI G_GTAC'C
Cuts at: 1783 1783
Size: 4763
MluI A'CGCG_T
Cuts at: 4196 4196
Size: 4763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 917 3522 3706 917
Size: 2605 184 1974
Fragments arranged by size:
2605 1974 184
MscI TGG'CCA
Cuts at: 988 1240 2524 988
Size: 252 1284 3227
Fragments arranged by size:
3227 1284 252
MslI CAynn'nnrTG
Cuts at: 356 1093 1675 2752 2879 356
Size: 737 582 1077 127 2240
Fragments arranged by size:
2240 1077 737 582 127
MspA1I CmG'CkG
Cuts at: 403 879 955 1233 1459 1874 2188 2548 3332
Size: 476 76 278 226 415 314 360 784
Cuts at: 3332 3577 403
Size: 245 1589
Fragments arranged by size:
1589 784 476 415 360 314 278 245
226 76
NaeI GCC'GGC
Cuts at: 2944 2944
Size: 4763
NarI GG'CG_CC
Cuts at: 2194 2442 2194
Size: 248 4515
NcoI C'CATG_G
Cuts at: 351 351
Size: 4763
NgoMIV G'CCGG_C
Cuts at: 2942 2942
Size: 4763
NspI r_CATG'y
Cuts at: 553 1816 2316 3919 553
Size: 1263 500 1603 1397
Fragments arranged by size:
1603 1397 1263 500
PciI A'CATG_T
Cuts at: 3915 3915
Size: 4763
PflMI CCAn_nnn'nTGG
Cuts at: 1236 1236
Size: 4763
PmeI GTTT'AAAC
Cuts at: 1762 1762
Size: 4763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 4731
PsiI TTA'TAA
Cuts at: 902 902
Size: 4763
PspXI vC'TCGA_Gb
Cuts at: 1356 1356
Size: 4763
PstI C_TGCA'G
Cuts at: 1810 2495 1810
Size: 685 4078
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4763
PvuII CAG'CTG
Cuts at: 403 879 2188 2548 403
Size: 476 1309 360 2618
Fragments arranged by size:
2618 1309 476 360
RsrII CG'GwC_CG
Cuts at: 459 2958 459
Size: 2499 2264
SacI G_AGCT'C
Cuts at: 1777 1777
Size: 4763
SalI G'TCGA_C
Cuts at: 1800 1800
Size: 4763
SapI GCTCTTCn'nnn_
Cuts at: 1732 2786 2996 1732
Size: 1054 210 3499
Fragments arranged by size:
3499 1054 210
SbfI CC_TGCA'GG
Cuts at: 1810 1810
Size: 4763
ScaI AGT'ACT
Cuts at: 4280 4280
Size: 4763
SfcI C'TryA_G
Cuts at: 33 515 1404 1806 2491 3459 3650 33
Size: 482 889 402 685 968 191 1146
Fragments arranged by size:
1146 968 889 685 482 402 191
SfoI GGC'GCC
Cuts at: 2195 2443 2195
Size: 248 4515
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
SmaI CCC'GGG
Cuts at: 1535 1785 1535
Size: 250 4513
SmlI C'TyrA_G
Cuts at: 313 1356 1918 2833 3547 3809 313
Size: 1043 562 915 714 262 1267
Fragments arranged by size:
1267 1043 915 714 562 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4763
SphI G_CATG'C
Cuts at: 553 1816 2316 553
Size: 1263 500 3000
Fragments arranged by size:
3000 1263 500
StyI C'CwwG_G
Cuts at: 351 849 1241 1897 2251 351
Size: 498 392 656 354 2863
Fragments arranged by size:
2863 656 498 392 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 193 401 447 623 1105 2346 2479 2938 3147
Size: 208 46 176 482 1241 133 459 209
Cuts at: 3147 193
Size: 1809
Fragments arranged by size:
1809 1241 482 459 209 208 176 133
46
TatI w'GTAC_w
Cuts at: 1442 4278 1442
Size: 2836 1927
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 121 153 357 389 4280 4312 121
Size: 32 204 32 3891 32 572
Fragments arranged by size:
3891 572 204 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 457 2560 457
Size: 2103 2660
XbaI T'CTAG_A
Cuts at: 1794 1794
Size: 4763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1275 2258 1275
Size: 983 3780
XhoI C'TCGA_G
Cuts at: 1356 1356
Size: 4763
XmaI C'CCGG_G
Cuts at: 1533 1783 1533
Size: 250 4513
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PsiI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmBI BspEI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI