Restriction map for
pF1KB3107
(Circular) (Six-base) MAPSORT of: pF1KB3107.seq Check: 8817 from: 1 to: 3608
pF1KB3107 3608 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 646 646
Size: 3608
Acc65I G'GTAC_C
Cuts at: 624 624
Size: 3608
AclI AA'CG_TT
Cuts at: 3448 3448
Size: 3608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 1433 1865 2218 2880 550
Size: 883 432 353 662 1278
Fragments arranged by size:
1278 883 662 432 353
AfeI AGC'GCT
Cuts at: 372 372
Size: 3608
AflII C'TTAA_G
Cuts at: 436 436
Size: 3608
AflIII A'CryG_T
Cuts at: 960 2760 3041 960
Size: 1800 281 1527
Fragments arranged by size:
1800 1527 281
AlwNI CAG_nnn'CTG
Cuts at: 913 2351 913
Size: 1438 2170
ApaLI G'TGCA_C
Cuts at: 2446 2446
Size: 3608
ApoI r'AATT_y
Cuts at: 558 612 2961 3217 558
Size: 54 2349 256 949
Fragments arranged by size:
2349 949 256 54
AseI AT'TA_AT
Cuts at: 20 20
Size: 3608
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3608
AvaI C'yCGr_G
Cuts at: 477 628 477
Size: 151 3457
BamHI G'GATC_C
Cuts at: 633 633
Size: 3608
BanI G'GyrC_C
Cuts at: 245 624 1038 1286 1321 245
Size: 379 414 248 35 2532
Fragments arranged by size:
2532 414 379 248 35
BanII G_rGCy'C
Cuts at: 622 1652 622
Size: 1030 2578
BbeI G_GCGC'C
Cuts at: 1042 1290 1042
Size: 248 3360
BciVI GTATCCnnnnn_n'
Cuts at: 1506 2562 3608 1506
Size: 1056 1046 1506
Fragments arranged by size:
1506 1056 1046
BclI T'GATC_A
Cuts at: 1128 1128
Size: 3608
BglII A'GATC_T
Cuts at: 1123 3160 1123
Size: 2037 1571
BlpI GC'TnA_GC
Cuts at: 720 720
Size: 3608
Bme1580I G_kGCm'C
Cuts at: 1233 1326 2450 1233
Size: 93 1124 2391
Fragments arranged by size:
2391 1124 93
BmgBI CAC'GTC
Cuts at: 293 293
Size: 3608
BmrI ACTGGGnnnn_n'
Cuts at: 820 901 1236 3380 820
Size: 81 335 2144 1048
Fragments arranged by size:
2144 1048 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1975 1975
Size: 3608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 784 1663 2377 2675 235
Size: 549 879 714 298 1168
Fragments arranged by size:
1168 879 714 549 298
BsaI GGTCTCn'nnnn_
Cuts at: 3312 3312
Size: 3608
BsaAI yAC'GTr
Cuts at: 961 961
Size: 3608
BsaBI GATnn'nnATC
Cuts at: 6 1122 3159 6
Size: 1116 2037 455
Fragments arranged by size:
2037 1116 455
BsaHI Gr'CG_yC
Cuts at: 1039 1287 1989 3485 1039
Size: 248 702 1496 1162
Fragments arranged by size:
1496 1162 702 248
BsaWI w'CCGG_w
Cuts at: 797 1318 1996 2407 2554 2828 797
Size: 521 678 411 147 274 1577
Fragments arranged by size:
1577 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 276 1033 1791 2456 2864 276
Size: 757 758 665 408 1020
Fragments arranged by size:
1020 758 757 665 408
BsiEI CG_ry'CG
Cuts at: 104 152 1150 1196 2426 104
Size: 48 998 46 1230 1286
Fragments arranged by size:
1286 1230 998 48 46
BsiHKAI G_wGCw'C
Cuts at: 259 622 1400 1590 2450 259
Size: 363 778 190 860 1417
Fragments arranged by size:
1417 860 778 363 190
Bsp1286I G_dGCh'C
Cuts at: 259 622 1233 1326 1400 1590 1652 2450 259
Size: 363 611 93 74 190 62 798 1417
Fragments arranged by size:
1417 798 611 363 190 93 74 62
BspHI T'CATG_A
Cuts at: 407 2040 407
Size: 1633 1975
BspMI ACCTGCnnnn'nnnn_
Cuts at: 466 658 872 1174 1555 466
Size: 192 214 302 381 2519
Fragments arranged by size:
2519 381 302 214 192
BsrBI CCG'CTC
Cuts at: 50 1900 1954 3438 3603 50
Size: 1850 54 1484 165 55
Fragments arranged by size:
1850 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 429 1520 429
Size: 1091 2517
BsrFI r'CCGG_y
Cuts at: 151 242 1606 1787 151
Size: 91 1364 181 1972
Fragments arranged by size:
1972 1364 181 91
BssSI C'ACGA_G
Cuts at: 1879 2587 1879
Size: 708 2900
BstAPI GCAn_nnn'nTGC
Cuts at: 374 985 374
Size: 611 2997
BstBI TT'CG_AA
Cuts at: 1969 1969
Size: 3608
BstYI r'GATC_y
Cuts at: 1 633 1100 1123 1458 1704 2010 2022 2108
Size: 632 467 23 335 246 306 12 86
Cuts at: 2108 3160 1
Size: 1052 449
Fragments arranged by size:
1052 632 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 221 107
Size: 114 3494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1558 1739 1902 1558
Size: 181 163 3264
Fragments arranged by size:
3264 181 163
BtsI GCAGTG_nn'
Cuts at: 851 851
Size: 3608
DraI TTT'AAA
Cuts at: 607 607
Size: 3608
DrdI GACnn_nn'nnGTC
Cuts at: 595 1314 2658 595
Size: 719 1344 1545
Fragments arranged by size:
1545 1344 719
EaeI y'GGCC_r
Cuts at: 149 549 1193 1367 1758 1785 3130 149
Size: 400 644 174 391 27 1345 627
Fragments arranged by size:
1345 644 627 400 391 174 27
EagI C'GGCC_G
Cuts at: 149 1193 149
Size: 1044 2564
EarI CTCTTCn'nnn_
Cuts at: 1631 1841 1631
Size: 210 3398
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1816 2558 2704 3418 1816
Size: 742 146 714 2006
Fragments arranged by size:
2006 742 714 146
EcoICRI GAG'CTC
Cuts at: 620 620
Size: 3608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 1433 1865 2218 2880 550
Size: 883 432 353 662 1278
Fragments arranged by size:
1278 883 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 747 747
Size: 3608
EcoRI G'AATT_C
Cuts at: 558 612 558
Size: 54 3554
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1627 1627
Size: 3608
FspI TGC'GCA
Cuts at: 177 1389 177
Size: 1212 2396
HaeII r_GCGC'y
Cuts at: 374 1042 1290 2520 3291 374
Size: 668 248 1230 771 691
Fragments arranged by size:
1230 771 691 668 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1450 1480 1482 1512 1450
Size: 30 2 30 3546
Fragments arranged by size:
3546 30 30 2
HincII GTy'rAC
Cuts at: 647 647
Size: 3608
KasI G'GCGC_C
Cuts at: 1038 1286 1038
Size: 248 3360
KpnI G_GTAC'C
Cuts at: 628 628
Size: 3608
MluI A'CGCG_T
Cuts at: 3041 3041
Size: 3608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2367 2551 2367
Size: 184 3424
MscI TGG'CCA
Cuts at: 551 1369 551
Size: 818 2790
MslI CAynn'nnrTG
Cuts at: 1597 1724 1597
Size: 127 3481
MspA1I CmG'CkG
Cuts at: 276 719 1033 1393 2177 2422 276
Size: 443 314 360 784 245 1462
Fragments arranged by size:
1462 784 443 360 314 245
NaeI GCC'GGC
Cuts at: 153 1789 153
Size: 1636 1972
NarI GG'CG_CC
Cuts at: 1039 1287 1039
Size: 248 3360
NcoI C'CATG_G
Cuts at: 107 221 107
Size: 114 3494
NgoMIV G'CCGG_C
Cuts at: 151 1787 151
Size: 1636 1972
NspI r_CATG'y
Cuts at: 452 661 1161 2764 452
Size: 209 500 1603 1296
Fragments arranged by size:
1603 1296 500 209
PciI A'CATG_T
Cuts at: 2760 2760
Size: 3608
PfoI T'CCnGG_A
Cuts at: 163 163
Size: 3608
PmeI GTTT'AAAC
Cuts at: 607 607
Size: 3608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2054 2086 2054
Size: 32 3576
PstI C_TGCA'G
Cuts at: 655 1340 655
Size: 685 2923
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3608
PvuII CAG'CTG
Cuts at: 276 1033 1393 276
Size: 757 360 2491
Fragments arranged by size:
2491 757 360
RsrII CG'GwC_CG
Cuts at: 201 1803 201
Size: 1602 2006
SacI G_AGCT'C
Cuts at: 622 622
Size: 3608
SalI G'TCGA_C
Cuts at: 645 645
Size: 3608
SapI GCTCTTCn'nnn_
Cuts at: 1631 1841 1631
Size: 210 3398
SbfI CC_TGCA'GG
Cuts at: 655 655
Size: 3608
ScaI AGT'ACT
Cuts at: 3125 3125
Size: 3608
SfcI C'TryA_G
Cuts at: 33 533 651 1336 2304 2495 33
Size: 500 118 685 968 191 1146
Fragments arranged by size:
1146 968 685 500 191 118
SfoI GGC'GCC
Cuts at: 1040 1288 1040
Size: 248 3360
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3608
SmaI CCC'GGG
Cuts at: 479 630 479
Size: 151 3457
SmlI C'TyrA_G
Cuts at: 250 436 763 1678 2392 2654 250
Size: 186 327 915 714 262 1204
Fragments arranged by size:
1204 915 714 327 262 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3608
SphI G_CATG'C
Cuts at: 661 1161 661
Size: 500 3108
StyI C'CwwG_G
Cuts at: 107 221 742 1096 107
Size: 114 521 354 2619
Fragments arranged by size:
2619 521 354 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1191 1324 1783 1992 1191
Size: 133 459 209 2807
Fragments arranged by size:
2807 459 209 133
TatI w'GTAC_w
Cuts at: 3123 3123
Size: 3608
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 3576
Tth111I GACn'n_nGTC
Cuts at: 1405 1405
Size: 3608
XbaI T'CTAG_A
Cuts at: 639 639
Size: 3608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1103 1103
Size: 3608
XmaI C'CCGG_G
Cuts at: 477 628 477
Size: 151 3457
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI