Restriction map for
pF1KB3109
(Circular) (Six-base) MAPSORT of: pF1KB3109.seq Check: 929 from: 1 to: 8471
pF1KB3109 8471 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3454 3454
Size: 8471
AatII G_ACGT'C
Cuts at: 1722 1722
Size: 8471
AccI GT'mk_AC
Cuts at: 1629 5509 1629
Size: 3880 4591
Acc65I G'GTAC_C
Cuts at: 5487 5487
Size: 8471
AclI AA'CG_TT
Cuts at: 8311 8311
Size: 8471
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 1100 4496 4526 4565 4774 5255 6296 6728
Size: 369 3396 30 39 209 481 1041 432
Cuts at: 6728 7475 7743 731
Size: 747 268 1459
Fragments arranged by size:
3396 1459 1041 747 481 432 369 268
209 39 30
AflII C'TTAA_G
Cuts at: 430 1605 5080 430
Size: 1175 3475 3821
Fragments arranged by size:
3821 3475 1175
AflIII A'CryG_T
Cuts at: 1303 5036 5823 6927 7904 1303
Size: 3733 787 1104 977 1870
Fragments arranged by size:
3733 1870 1104 977 787
AgeI A'CCGG_T
Cuts at: 6859 6859
Size: 8471
AhdI GACnn_n'nnGTC
Cuts at: 536 536
Size: 8471
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4817 4817
Size: 8471
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 166 198 5014 5046 166
Size: 32 4816 32 3591
Fragments arranged by size:
4816 3591 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1587 2297 2746 2903 4285 4983 5017 5776 7343
Size: 710 449 157 1382 698 34 759 1567
Cuts at: 7343 1587
Size: 2715
Fragments arranged by size:
2715 1567 1382 759 710 698 449 157
34
ApaLI G'TGCA_C
Cuts at: 2525 3600 7241 2525
Size: 1075 3641 3755
Fragments arranged by size:
3755 3641 1075
ApoI r'AATT_y
Cuts at: 496 783 1334 4027 5214 5475 7824 8080 496
Size: 287 551 2693 1187 261 2349 256 887
Fragments arranged by size:
2693 2349 1187 887 551 287 261 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8471
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8471
AvaI C'yCGr_G
Cuts at: 158 178 3615 5491 158
Size: 20 3437 1876 3138
Fragments arranged by size:
3437 3138 1876 20
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2307 2340 2946 2979 3144 3177 2307
Size: 33 606 33 165 33 7601
Fragments arranged by size:
7601 606 165 33 33 33
BamHI G'GATC_C
Cuts at: 5496 5496
Size: 8471
BanI G'GyrC_C
Cuts at: 3208 4054 5487 5901 6149 6184 3208
Size: 846 1433 414 248 35 5495
Fragments arranged by size:
5495 1433 846 414 248 35
BanII G_rGCy'C
Cuts at: 159 2197 3820 5485 6515 159
Size: 2038 1623 1665 1030 2115
Fragments arranged by size:
2115 2038 1665 1623 1030
BbeI G_GCGC'C
Cuts at: 5905 6153 5905
Size: 248 8223
BbsI GAAGACnn'nnnn_
Cuts at: 3268 3615 4184 4553 4567 4967 5176 5357 3268
Size: 347 569 369 14 400 209 181 6382
Fragments arranged by size:
6382 569 400 369 347 209 181 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 899 933 2644 2678 3163 3197 899
Size: 34 1711 34 485 34 6173
Fragments arranged by size:
6173 1711 485 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 770 2947 2983 3145 6369 7130 8471 770
Size: 2177 36 162 3224 761 1341 770
Fragments arranged by size:
3224 2177 1341 770 761 162 36
BclI T'GATC_A
Cuts at: 3671 5991 3671
Size: 2320 6151
BglII A'GATC_T
Cuts at: 5986 8023 5986
Size: 2037 6434
BlpI GC'TnA_GC
Cuts at: 5583 5583
Size: 8471
Bme1580I G_kGCm'C
Cuts at: 951 2529 2681 3211 3604 4470 6096 6189 7245
Size: 1578 152 530 393 866 1626 93 1056
Cuts at: 7245 951
Size: 2177
Fragments arranged by size:
2177 1626 1578 1056 866 530 393 152
93
BmgBI CAC'GTC
Cuts at: 272 2187 3486 4182 272
Size: 1915 1299 696 4561
Fragments arranged by size:
4561 1915 1299 696
BmrI ACTGGGnnnn_n'
Cuts at: 1608 1986 2546 2691 2734 2756 3098 4197 5683
Size: 378 560 145 43 22 342 1099 1486
Cuts at: 5683 5764 6099 8243 1608
Size: 81 335 2144 1836
Fragments arranged by size:
2144 1836 1486 1099 560 378 342 335
145 81 43 22
BmtI G_CTAG'C
Cuts at: 130 130
Size: 8471
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6838 6838
Size: 8471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 1529 1880 3730 103
Size: 1426 351 1850 4844
Fragments arranged by size:
4844 1850 1426 351
Bpu10I CC'TnA_GC
Cuts at: 170 170
Size: 8471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 825 2073 3085 3887 4114 4757 5285 5366 5647
Size: 1248 1012 802 227 643 528 81 281
Cuts at: 5647 6526 7018 7316 825
Size: 879 492 298 1980
Fragments arranged by size:
1980 1248 1012 879 802 643 528 492
298 281 227 81
BsaI GGTCTCn'nnnn_
Cuts at: 361 1065 1372 3470 4506 8175 361
Size: 704 307 2098 1036 3669 657
Fragments arranged by size:
3669 2098 1036 704 657 307
BsaAI yAC'GTr
Cuts at: 1304 4750 5824 1304
Size: 3446 1074 3951
Fragments arranged by size:
3951 3446 1074
BsaBI GATnn'nnATC
Cuts at: 6 359 865 5985 8022 6
Size: 353 506 5120 2037 455
Fragments arranged by size:
5120 2037 506 455 353
BsaHI Gr'CG_yC
Cuts at: 1719 2886 3529 5902 6150 6852 8348 1719
Size: 1167 643 2373 248 702 1496 1842
Fragments arranged by size:
2373 1842 1496 1167 702 643 248
BsaWI w'CCGG_w
Cuts at: 1841 5660 6181 6859 7133 7280 7691 1841
Size: 3819 521 678 274 147 411 2621
Fragments arranged by size:
3819 2621 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 402 432 2264 2294 402
Size: 30 1832 30 6579
Fragments arranged by size:
6579 1832 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2163 4117 4237 4615 2163
Size: 1954 120 378 6019
Fragments arranged by size:
6019 1954 378 120
BseYI C'CCAG_C
Cuts at: 1311 5042 5896 6654 7231 7727 1311
Size: 3731 854 758 577 496 2055
Fragments arranged by size:
3731 2055 854 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 265 1026 2020 3618 5174 171
Size: 94 761 994 1598 1556 3468
Fragments arranged by size:
3468 1598 1556 994 761 94
BsiEI CG_ry'CG
Cuts at: 104 243 565 2113 6013 6059 7267 104
Size: 139 322 1548 3900 46 1208 1308
Fragments arranged by size:
3900 1548 1308 1208 322 139 46
BsiHKAI G_wGCw'C
Cuts at: 249 1598 1696 2529 3224 3604 5485 6263 6453
Size: 1349 98 833 695 380 1881 778 190
Cuts at: 6453 7245 249
Size: 792 1475
Fragments arranged by size:
1881 1475 1349 833 792 778 695 380
190 98
BsmI GAATG_Cn'
Cuts at: 2434 4119 2434
Size: 1685 6786
BsmBI CGTCTCn'nnnn_
Cuts at: 3520 3520
Size: 8471
Bsp1286I G_dGCh'C
Cuts at: 159 249 951 1598 1696 2197 2529 2681 3211
Size: 90 702 647 98 501 332 152 530
Cuts at: 3211 3224 3604 3820 4470 5485 6096 6189 6263
Size: 13 380 216 650 1015 611 93 74
Cuts at: 6263 6453 6515 7245 159
Size: 190 62 730 1385
Fragments arranged by size:
1385 1015 730 702 650 647 611 530
501 380 332 216 190 152 98 93
90 74 62 13
BspEI T'CCGG_A
Cuts at: 7691 7691
Size: 8471
BspHI T'CATG_A
Cuts at: 363 4160 7647 363
Size: 3797 3487 1187
Fragments arranged by size:
3797 3487 1187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1083 2521 3454 4991 5521 5735 6037 6418 1083
Size: 1438 933 1537 530 214 302 381 3136
Fragments arranged by size:
3136 1537 1438 933 530 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1824 6763 6817 8301 8466 50
Size: 1774 4939 54 1484 165 55
Fragments arranged by size:
4939 1774 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1125 1370 1617 2721 3372 3402 3674 4602 4808
Size: 245 247 1104 651 30 272 928 206
Cuts at: 4808 6383 1125
Size: 1575 3213
Fragments arranged by size:
3213 1575 1104 928 651 272 247 245
206 30
BsrFI r'CCGG_y
Cuts at: 1700 1864 2112 2489 3143 6469 6650 6859 1700
Size: 164 248 377 654 3326 181 209 3312
Fragments arranged by size:
3326 3312 654 377 248 209 181 164
BssHII G'CGCG_C
Cuts at: 165 165
Size: 8471
BssSI C'ACGA_G
Cuts at: 6742 7100 6742
Size: 358 8113
BstAPI GCAn_nnn'nTGC
Cuts at: 3455 3641 5848 3455
Size: 186 2207 6078
Fragments arranged by size:
6078 2207 186
BstBI TT'CG_AA
Cuts at: 6832 6832
Size: 8471
BstEII G'GTnAC_C
Cuts at: 261 2283 261
Size: 2022 6449
BstXI CCAn_nnnn'nTGG
Cuts at: 1598 2716 3960 4446 1598
Size: 1118 1244 486 5623
Fragments arranged by size:
5623 1244 1118 486
BstYI r'GATC_y
Cuts at: 1 354 738 759 1278 1458 2587 2913 5496
Size: 353 384 21 519 180 1129 326 2583
Cuts at: 5496 5963 5986 6321 6567 7579 7665 7677 8023
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8023 1
Size: 449
Fragments arranged by size:
2583 1129 1012 519 467 449 384 353
346 335 326 246 180 86 23 21
12
Bsu36I CC'TnA_GG
Cuts at: 2438 2985 2438
Size: 547 7924
BtgI C'CryG_G
Cuts at: 107 221 1166 1214 1928 2258 2777 3548 4439
Size: 114 945 48 714 330 519 771 891
Cuts at: 4439 107
Size: 4139
Fragments arranged by size:
4139 945 891 771 714 519 330 114
48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6421 6602 6765 6421
Size: 181 163 8127
Fragments arranged by size:
8127 181 163
BtsI GCAGTG_nn'
Cuts at: 996 1240 1458 1539 2662 2844 3418 4877 5714
Size: 244 218 81 1123 182 574 1459 837
Cuts at: 5714 996
Size: 3753
Fragments arranged by size:
3753 1459 1123 837 574 244 218 182
81
ClaI AT'CG_AT
Cuts at: 2210 2210
Size: 8471
DraI TTT'AAA
Cuts at: 5470 5470
Size: 8471
DraIII CAC_nnn'GTG
Cuts at: 3538 3592 3538
Size: 54 8417
DrdI GACnn_nn'nnGTC
Cuts at: 4456 6177 7035 4456
Size: 1721 858 5892
Fragments arranged by size:
5892 1721 858
EaeI y'GGCC_r
Cuts at: 240 1163 1377 2110 2619 2718 3332 6056 6230
Size: 923 214 733 509 99 614 2724 174
Cuts at: 6230 6621 6648 7993 240
Size: 391 27 1345 718
Fragments arranged by size:
2724 1345 923 733 718 614 509 391
214 174 99 27
EagI C'GGCC_G
Cuts at: 240 2110 6056 240
Size: 1870 3946 2655
Fragments arranged by size:
3946 2655 1870
EarI CTCTTCn'nnn_
Cuts at: 3472 5231 6494 6704 3472
Size: 1759 1263 210 5239
Fragments arranged by size:
5239 1759 1263 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6679 6989 7135 8281 6679
Size: 310 146 1146 6869
Fragments arranged by size:
6869 1146 310 146
EcoICRI GAG'CTC
Cuts at: 5483 5483
Size: 8471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 731 1100 1529 1880 3730 4496 4526 4565
Size: 628 369 429 351 1850 766 30 39
Cuts at: 4565 4774 5255 6296 6728 7475 7743 103
Size: 209 481 1041 432 747 268 831
Fragments arranged by size:
1850 1041 831 766 747 628 481 432
429 369 351 268 209 39 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1079 3172 1079
Size: 2093 6378
EcoO109I rG'GnC_Cy
Cuts at: 1801 4316 4387 5610 1801
Size: 2515 71 1223 4662
Fragments arranged by size:
4662 2515 1223 71
EcoRI G'AATT_C
Cuts at: 496 5475 496
Size: 4979 3492
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6490 6490
Size: 8471
FspI TGC'GCA
Cuts at: 198 6252 198
Size: 6054 2417
HaeII r_GCGC'y
Cuts at: 5905 6153 7175 8154 5905
Size: 248 1022 979 6222
Fragments arranged by size:
6222 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 360 392 522 554 1704 1736 2084 2116 2199
Size: 32 130 32 1150 32 348 32 83
Cuts at: 2199 2231 2968 3000 4183 4215 6313 6343 6345
Size: 32 737 32 1183 32 2098 30 2
Cuts at: 6345 6375 360
Size: 30 2456
Fragments arranged by size:
2456 2098 1183 1150 737 348 130 83
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1630 5510 1630
Size: 3880 4591
HindIII A'AGCT_T
Cuts at: 253 253
Size: 8471
KasI G'GCGC_C
Cuts at: 5901 6149 5901
Size: 248 8223
KpnI G_GTAC'C
Cuts at: 5491 5491
Size: 8471
MluI A'CGCG_T
Cuts at: 5036 7904 5036
Size: 2868 5603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 501 2044 2132 2484 7142 7326 501
Size: 1543 88 352 4658 184 1646
Fragments arranged by size:
4658 1646 1543 352 184 88
MscI TGG'CCA
Cuts at: 1165 1379 2621 2720 3334 6232 1165
Size: 214 1242 99 614 2898 3404
Fragments arranged by size:
3404 2898 1242 614 214 99
MslI CAynn'nnrTG
Cuts at: 1418 1996 2726 2789 6460 6587 1418
Size: 578 730 63 3671 127 3302
Fragments arranged by size:
3671 3302 730 578 127 63
MspA1I CmG'CkG
Cuts at: 209 544 1181 1315 3308 3330 4285 5245 5582
Size: 335 637 134 1993 22 955 960 337
Cuts at: 5582 5896 6256 7269 7514 209
Size: 314 360 1013 245 1166
Fragments arranged by size:
1993 1166 1013 960 955 637 360 337
335 314 245 134 22
NaeI GCC'GGC
Cuts at: 1702 1866 2491 6652 1702
Size: 164 625 4161 3521
Fragments arranged by size:
4161 3521 625 164
NarI GG'CG_CC
Cuts at: 5902 6150 5902
Size: 248 8223
NcoI C'CATG_G
Cuts at: 107 1214 1928 2777 4439 107
Size: 1107 714 849 1662 4139
Fragments arranged by size:
4139 1662 1107 849 714
NdeI CA'TA_TG
Cuts at: 340 340
Size: 8471
NgoMIV G'CCGG_C
Cuts at: 1700 1864 2489 6650 1700
Size: 164 625 4161 3521
Fragments arranged by size:
4161 3521 625 164
NheI G'CTAG_C
Cuts at: 126 126
Size: 8471
NspI r_CATG'y
Cuts at: 1005 3426 5462 5524 6024 6931 1005
Size: 2421 2036 62 500 907 2545
Fragments arranged by size:
2545 2421 2036 907 500 62
PasI CC'CwG_GG
Cuts at: 3515 4312 3515
Size: 797 7674
PciI A'CATG_T
Cuts at: 6927 6927
Size: 8471
PflMI CCAn_nnn'nTGG
Cuts at: 2746 3323 2746
Size: 577 7894
PfoI T'CCnGG_A
Cuts at: 856 1506 1835 1852 2949 3707 856
Size: 650 329 17 1097 758 5620
Fragments arranged by size:
5620 1097 758 650 329 17
PmeI GTTT'AAAC
Cuts at: 5470 5470
Size: 8471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7610 7642 7610
Size: 32 8439
PstI C_TGCA'G
Cuts at: 3451 4357 4884 5518 6203 3451
Size: 906 527 634 685 5719
Fragments arranged by size:
5719 906 685 634 527
PvuI CG_AT'CG
Cuts at: 104 565 104
Size: 461 8010
PvuII CAG'CTG
Cuts at: 209 544 1315 3330 5245 5896 6256 209
Size: 335 771 2015 1915 651 360 2424
Fragments arranged by size:
2424 2015 1915 771 651 360 335
RsrII CG'GwC_CG
Cuts at: 6666 6666
Size: 8471
SacI G_AGCT'C
Cuts at: 5485 5485
Size: 8471
SalI G'TCGA_C
Cuts at: 1628 5508 1628
Size: 3880 4591
SapI GCTCTTCn'nnn_
Cuts at: 6494 6704 6494
Size: 210 8261
SbfI CC_TGCA'GG
Cuts at: 5518 5518
Size: 8471
ScaI AGT'ACT
Cuts at: 7988 7988
Size: 8471
SexAI A'CCwGG_T
Cuts at: 1740 2063 1740
Size: 323 8148
SfcI C'TryA_G
Cuts at: 33 3447 3970 4353 4880 5514 6199 7192 7383
Size: 3414 523 383 527 634 685 993 191
Cuts at: 7383 33
Size: 1121
Fragments arranged by size:
3414 1121 993 685 634 527 523 383
191
SfoI GGC'GCC
Cuts at: 5903 6151 5903
Size: 248 8223
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8471
SmaI CCC'GGG
Cuts at: 5493 5493
Size: 8471
SmlI C'TyrA_G
Cuts at: 430 804 1605 2088 3100 3902 4093 4772 5080
Size: 374 801 483 1012 802 191 679 308
Cuts at: 5080 5300 5381 5626 6541 7033 7295 430
Size: 220 81 245 915 492 262 1606
Fragments arranged by size:
1606 1012 915 802 801 679 492 483
374 308 262 245 220 191 81
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8471
SphI G_CATG'C
Cuts at: 3426 5524 6024 3426
Size: 2098 500 5873
Fragments arranged by size:
5873 2098 500
StuI AGG'CCT
Cuts at: 622 2417 3724 622
Size: 1795 1307 5369
Fragments arranged by size:
5369 1795 1307
StyI C'CwwG_G
Cuts at: 107 772 1019 1066 1214 1647 1928 2777 3719
Size: 665 247 47 148 433 281 849 942
Cuts at: 3719 4058 4439 5605 5959 107
Size: 339 381 1166 354 2619
Fragments arranged by size:
2619 1166 942 849 665 433 381 354
339 281 247 148 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1274 6054 6187 6646 6855 1274
Size: 4780 133 459 209 2890
Fragments arranged by size:
4780 2890 459 209 133
TatI w'GTAC_w
Cuts at: 2165 7986 2165
Size: 5821 2650
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2264 2296 3689 3721 7988 8020 2264
Size: 32 1393 32 4267 32 2715
Fragments arranged by size:
4267 2715 1393 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6268 6268
Size: 8471
XbaI T'CTAG_A
Cuts at: 2325 5502 2325
Size: 3177 5294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3424 4140 4581 5966 3424
Size: 716 441 1385 5929
Fragments arranged by size:
5929 1385 716 441
XmaI C'CCGG_G
Cuts at: 5491 5491
Size: 8471
XmnI GAAnn'nnTTC
Cuts at: 2306 4550 4964 2306
Size: 2244 414 5813
Fragments arranged by size:
5813 2244 414
ZraI GAC'GTC
Cuts at: 1720 1720
Size: 8471
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AleI ApaI AscI AvrII BbvCI BfrBI BglI
BsiWI BsrGI BstZ17I CspCI EcoRV FseI FspAI HpaI
MfeI NotI NruI NsiI PacI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI