Restriction map for
pF1KB3111
(Circular) (Six-base) MAPSORT of: pF1KB3111.seq Check: 7253 from: 1 to: 4196
pF1KB3111 4196 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 667 667
Size: 4196
AccI GT'mk_AC
Cuts at: 390 1234 390
Size: 844 3352
Acc65I G'GTAC_C
Cuts at: 1212 1212
Size: 4196
AclI AA'CG_TT
Cuts at: 4036 4036
Size: 4196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2021 2453 3200 3468 2021
Size: 432 747 268 2749
Fragments arranged by size:
2749 747 432 268
AflIII A'CryG_T
Cuts at: 1548 2652 3629 1548
Size: 1104 977 2115
Fragments arranged by size:
2115 1104 977
AgeI A'CCGG_T
Cuts at: 115 2584 115
Size: 2469 1727
AhdI GACnn_n'nnGTC
Cuts at: 397 397
Size: 4196
AleI CACnn'nnGTG
Cuts at: 118 118
Size: 4196
AlwNI CAG_nnn'CTG
Cuts at: 1501 3068 1501
Size: 1567 2629
ApaLI G'TGCA_C
Cuts at: 239 2966 239
Size: 2727 1469
ApoI r'AATT_y
Cuts at: 1200 3549 3805 1200
Size: 2349 256 1591
Fragments arranged by size:
2349 1591 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
AvaI C'yCGr_G
Cuts at: 1216 1216
Size: 4196
BamHI G'GATC_C
Cuts at: 1221 1221
Size: 4196
BanI G'GyrC_C
Cuts at: 1015 1212 1626 1874 1909 1015
Size: 197 414 248 35 3302
Fragments arranged by size:
3302 414 248 197 35
BanII G_rGCy'C
Cuts at: 495 1210 2240 495
Size: 715 1030 2451
Fragments arranged by size:
2451 1030 715
BbeI G_GCGC'C
Cuts at: 1019 1630 1878 1019
Size: 611 248 3337
Fragments arranged by size:
3337 611 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 174 208 174
Size: 34 4162
BciVI GTATCCnnnnn_n'
Cuts at: 2094 2855 4196 2094
Size: 761 1341 2094
Fragments arranged by size:
2094 1341 761
BclI T'GATC_A
Cuts at: 329 1716 329
Size: 1387 2809
BfrBI ATG'CAT
Cuts at: 168 943 168
Size: 775 3421
BglII A'GATC_T
Cuts at: 590 1711 3748 590
Size: 1121 2037 1038
Fragments arranged by size:
2037 1121 1038
BlpI GC'TnA_GC
Cuts at: 1308 1308
Size: 4196
Bme1580I G_kGCm'C
Cuts at: 243 1821 1914 2970 243
Size: 1578 93 1056 1469
Fragments arranged by size:
1578 1469 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1066 1408 1489 1824 3968 1066
Size: 342 81 335 2144 1294
Fragments arranged by size:
2144 1294 342 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 4196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 896 781
Size: 115 4081
Bpu10I CC'TnA_GC
Cuts at: 494 494
Size: 4196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1372 2251 2743 3041 1372
Size: 879 492 298 2527
Fragments arranged by size:
2527 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3900 3900
Size: 4196
BsaAI yAC'GTr
Cuts at: 244 1549 244
Size: 1305 2891
BsaBI GATnn'nnATC
Cuts at: 6 1710 3747 6
Size: 1704 2037 455
Fragments arranged by size:
2037 1704 455
BsaHI Gr'CG_yC
Cuts at: 1008 1016 1627 1875 2577 4073 1008
Size: 8 611 248 702 1496 1131
Fragments arranged by size:
1496 1131 702 611 248 8
BsaWI w'CCGG_w
Cuts at: 115 663 883 1385 1906 2584 2858 3005 3416
Size: 548 220 502 521 678 274 147 411
Cuts at: 3416 115
Size: 895
Fragments arranged by size:
895 678 548 521 502 411 274 220
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 197 227 197
Size: 30 4166
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 244
Size: 4196
BseYI C'CCAG_C
Cuts at: 1621 2379 2956 3452 1621
Size: 758 577 496 2365
Fragments arranged by size:
2365 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 817
Size: 4196
BsiEI CG_ry'CG
Cuts at: 104 1738 1784 2992 104
Size: 1634 46 1208 1308
Fragments arranged by size:
1634 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 243 1210 1988 2178 2970 243
Size: 967 778 190 792 1469
Fragments arranged by size:
1469 967 792 778 190
BsmI GAATG_Cn'
Cuts at: 557 557
Size: 4196
BsmBI CGTCTCn'nnnn_
Cuts at: 1162 1162
Size: 4196
Bsp1286I G_dGCh'C
Cuts at: 243 495 1210 1821 1914 1988 2178 2240 2970
Size: 252 715 611 93 74 190 62 730
Cuts at: 2970 243
Size: 1469
Fragments arranged by size:
1469 730 715 611 252 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3416 3416
Size: 4196
BspHI T'CATG_A
Cuts at: 963 3372 963
Size: 2409 1787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 667 1086 1246 1460 1762 2143 667
Size: 419 160 214 302 381 2720
Fragments arranged by size:
2720 419 381 302 214 160
BsrBI CCG'CTC
Cuts at: 50 537 2488 2542 4026 4191 50
Size: 487 1951 54 1484 165 55
Fragments arranged by size:
1951 1484 487 165 55 54
BsrDI GCAATG_nn'
Cuts at: 851 2108 851
Size: 1257 2939
BsrFI r'CCGG_y
Cuts at: 115 641 2194 2375 2584 115
Size: 526 1553 181 209 1727
Fragments arranged by size:
1727 1553 526 209 181
BssSI C'ACGA_G
Cuts at: 2467 2825 2467
Size: 358 3838
BstAPI GCAn_nnn'nTGC
Cuts at: 1573 1573
Size: 4196
BstBI TT'CG_AA
Cuts at: 2557 2557
Size: 4196
BstEII G'GTnAC_C
Cuts at: 399 1098 399
Size: 699 3497
BstYI r'GATC_y
Cuts at: 1 590 1031 1081 1111 1221 1688 1711 2046
Size: 589 441 50 30 110 467 23 335
Cuts at: 2046 2292 3304 3390 3402 3748 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 589 467 449 441 346 335 246
110 86 50 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 406 406
Size: 4196
BtgI C'CryG_G
Cuts at: 107 107
Size: 4196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2146 2327 2490 2146
Size: 181 163 3852
Fragments arranged by size:
3852 181 163
BtsI GCAGTG_nn'
Cuts at: 1439 1439
Size: 4196
DraI TTT'AAA
Cuts at: 1195 1195
Size: 4196
DrdI GACnn_nn'nnGTC
Cuts at: 1902 2760 1902
Size: 858 3338
EaeI y'GGCC_r
Cuts at: 1781 1955 2346 2373 3718 1781
Size: 174 391 27 1345 2259
Fragments arranged by size:
2259 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1781 1781
Size: 4196
EarI CTCTTCn'nnn_
Cuts at: 341 2219 2429 341
Size: 1878 210 2108
Fragments arranged by size:
2108 1878 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2404 2714 2860 4006 2404
Size: 310 146 1146 2594
Fragments arranged by size:
2594 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1208 1208
Size: 4196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 896 2021 2453 3200 3468 781
Size: 115 1125 432 747 268 1509
Fragments arranged by size:
1509 1125 747 432 268 115
EcoO109I rG'GnC_Cy
Cuts at: 1335 1335
Size: 4196
EcoRI G'AATT_C
Cuts at: 1200 1200
Size: 4196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 4196
FspI TGC'GCA
Cuts at: 1977 1977
Size: 4196
HaeII r_GCGC'y
Cuts at: 1019 1630 1878 2900 3879 1019
Size: 611 248 1022 979 1336
Fragments arranged by size:
1336 1022 979 611 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 125 157 383 415 449 481 945 977 2038
Size: 32 226 32 34 32 464 32 1061
Cuts at: 2038 2068 2070 2100 125
Size: 30 2 30 2221
Fragments arranged by size:
2221 1061 464 226 34 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1235 1235
Size: 4196
KasI G'GCGC_C
Cuts at: 1015 1626 1874 1015
Size: 611 248 3337
Fragments arranged by size:
3337 611 248
KpnI G_GTAC'C
Cuts at: 1216 1216
Size: 4196
MluI A'CGCG_T
Cuts at: 3629 3629
Size: 4196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 587 2867 3051 587
Size: 2280 184 1732
Fragments arranged by size:
2280 1732 184
MscI TGG'CCA
Cuts at: 1957 1957
Size: 4196
MslI CAynn'nnrTG
Cuts at: 118 2185 2312 118
Size: 2067 127 2002
Fragments arranged by size:
2067 2002 127
MspA1I CmG'CkG
Cuts at: 382 1307 1621 1981 2994 3239 382
Size: 925 314 360 1013 245 1339
Fragments arranged by size:
1339 1013 925 360 314 245
NaeI GCC'GGC
Cuts at: 2377 2377
Size: 4196
NarI GG'CG_CC
Cuts at: 1016 1627 1875 1016
Size: 611 248 3337
Fragments arranged by size:
3337 611 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4196
NgoMIV G'CCGG_C
Cuts at: 2375 2375
Size: 4196
NsiI A_TGCA'T
Cuts at: 170 945 170
Size: 775 3421
NspI r_CATG'y
Cuts at: 1249 1749 2656 1249
Size: 500 907 2789
Fragments arranged by size:
2789 907 500
PciI A'CATG_T
Cuts at: 2652 2652
Size: 4196
PmeI GTTT'AAAC
Cuts at: 1195 1195
Size: 4196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3335 3367 3335
Size: 32 4164
PstI C_TGCA'G
Cuts at: 1243 1928 1243
Size: 685 3511
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4196
PvuII CAG'CTG
Cuts at: 382 1621 1981 382
Size: 1239 360 2597
Fragments arranged by size:
2597 1239 360
RsrII CG'GwC_CG
Cuts at: 2391 2391
Size: 4196
SacI G_AGCT'C
Cuts at: 1210 1210
Size: 4196
SalI G'TCGA_C
Cuts at: 1233 1233
Size: 4196
SapI GCTCTTCn'nnn_
Cuts at: 2219 2429 2219
Size: 210 3986
SbfI CC_TGCA'GG
Cuts at: 1243 1243
Size: 4196
ScaI AGT'ACT
Cuts at: 3713 3713
Size: 4196
SfcI C'TryA_G
Cuts at: 33 1239 1924 2917 3108 33
Size: 1206 685 993 191 1121
Fragments arranged by size:
1206 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1017 1628 1876 1017
Size: 611 248 3337
Fragments arranged by size:
3337 611 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
SgrAI Cr'CCGG_yG
Cuts at: 115 115
Size: 4196
SmaI CCC'GGG
Cuts at: 1218 1218
Size: 4196
SmlI C'TyrA_G
Cuts at: 1351 2266 2758 3020 1351
Size: 915 492 262 2527
Fragments arranged by size:
2527 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4196
SphI G_CATG'C
Cuts at: 1249 1749 1249
Size: 500 3696
StuI AGG'CCT
Cuts at: 781 781
Size: 4196
StyI C'CwwG_G
Cuts at: 107 1330 1684 107
Size: 1223 354 2619
Fragments arranged by size:
2619 1223 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1779 1912 2371 2580 1779
Size: 133 459 209 3395
Fragments arranged by size:
3395 459 209 133
TatI w'GTAC_w
Cuts at: 484 3711 484
Size: 3227 969
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3713 3745 3713
Size: 32 4164
Tth111I GACn'n_nGTC
Cuts at: 1993 1993
Size: 4196
XbaI T'CTAG_A
Cuts at: 774 1227 774
Size: 453 3743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1128 1691 1128
Size: 563 3633
XmaI C'CCGG_G
Cuts at: 1216 1216
Size: 4196
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BglI BmgBI BmtI BsiWI BsrGI
BssHII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI