Restriction map for
pF1KB3114
(Circular) (Six-base) MAPSORT of: pF1KB3114.seq Check: 6704 from: 1 to: 4748
pF1KB3114 4748 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 257 1786 257
Size: 1529 3219
Acc65I G'GTAC_C
Cuts at: 1764 1764
Size: 4748
AclI AA'CG_TT
Cuts at: 4588 4588
Size: 4748
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 401 655 1175 1618 1736 2573 3005 3358
Size: 42 254 520 443 118 837 432 353
Cuts at: 3358 4020 359
Size: 662 1087
Fragments arranged by size:
1087 837 662 520 443 432 353 254
118 42
AflIII A'CryG_T
Cuts at: 1100 2100 3900 4181 1100
Size: 1000 1800 281 1667
Fragments arranged by size:
1800 1667 1000 281
AleI CACnn'nnGTG
Cuts at: 986 1495 986
Size: 509 4239
AlwNI CAG_nnn'CTG
Cuts at: 198 553 2053 3491 198
Size: 355 1500 1438 1455
Fragments arranged by size:
1500 1455 1438 355
ApaLI G'TGCA_C
Cuts at: 3586 3586
Size: 4748
ApoI r'AATT_y
Cuts at: 457 491 851 1035 1752 4101 4357 457
Size: 34 360 184 717 2349 256 848
Fragments arranged by size:
2349 848 717 360 256 184 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 4748
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
AvaI C'yCGr_G
Cuts at: 1768 1768
Size: 4748
BamHI G'GATC_C
Cuts at: 1773 1773
Size: 4748
BanI G'GyrC_C
Cuts at: 1764 2178 2426 2461 1764
Size: 414 248 35 4051
Fragments arranged by size:
4051 414 248 35
BanII G_rGCy'C
Cuts at: 350 1762 2792 350
Size: 1412 1030 2306
Fragments arranged by size:
2306 1412 1030
BbeI G_GCGC'C
Cuts at: 2182 2430 2182
Size: 248 4500
BbsI GAAGACnn'nnnn_
Cuts at: 330 1706 330
Size: 1376 3372
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 583 617 583
Size: 34 4714
BciVI GTATCCnnnnn_n'
Cuts at: 327 2646 3702 4748 327
Size: 2319 1056 1046 327
Fragments arranged by size:
2319 1056 1046 327
BclI T'GATC_A
Cuts at: 2268 2268
Size: 4748
BglII A'GATC_T
Cuts at: 2263 4300 2263
Size: 2037 2711
BlpI GC'TnA_GC
Cuts at: 1860 1860
Size: 4748
Bme1580I G_kGCm'C
Cuts at: 2373 2466 3590 2373
Size: 93 1124 3531
Fragments arranged by size:
3531 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1344 1960 2041 2376 4520 1344
Size: 616 81 335 2144 1572
Fragments arranged by size:
2144 1572 616 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1464 3115 1464
Size: 1651 3097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1074 1074
Size: 4748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 1924 2803 3517 3815 991
Size: 933 879 714 298 1924
Fragments arranged by size:
1924 933 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4452 4452
Size: 4748
BsaAI yAC'GTr
Cuts at: 984 2101 984
Size: 1117 3631
BsaBI GATnn'nnATC
Cuts at: 6 2262 4299 6
Size: 2256 2037 455
Fragments arranged by size:
2256 2037 455
BsaHI Gr'CG_yC
Cuts at: 2179 2427 3129 4625 2179
Size: 248 702 1496 2302
Fragments arranged by size:
2302 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1937 2458 3136 3547 3694 3968 1937
Size: 521 678 411 147 274 2717
Fragments arranged by size:
2717 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1279 1279
Size: 4748
BseYI C'CCAG_C
Cuts at: 1215 2173 2931 3596 4004 1215
Size: 958 758 665 408 1959
Fragments arranged by size:
1959 958 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1010 1649 1010
Size: 639 4109
BsiEI CG_ry'CG
Cuts at: 104 2290 2336 3566 104
Size: 2186 46 1230 1286
Fragments arranged by size:
2186 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 350 1762 2540 2730 3590 350
Size: 1412 778 190 860 1508
Fragments arranged by size:
1508 1412 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 350 1762 2373 2466 2540 2730 2792 3590 350
Size: 1412 611 93 74 190 62 798 1508
Fragments arranged by size:
1508 1412 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 882 3180 882
Size: 2298 2450
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1798 2012 2314 2695 1798
Size: 214 302 381 3851
Fragments arranged by size:
3851 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3040 3094 4578 4743 50
Size: 2990 54 1484 165 55
Fragments arranged by size:
2990 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 866 1508 2660 866
Size: 642 1152 2954
Fragments arranged by size:
2954 1152 642
BsrFI r'CCGG_y
Cuts at: 2746 2927 2746
Size: 181 4567
BssSI C'ACGA_G
Cuts at: 3019 3727 3019
Size: 708 4040
BstAPI GCAn_nnn'nTGC
Cuts at: 2125 2125
Size: 4748
BstBI TT'CG_AA
Cuts at: 3109 3109
Size: 4748
BstXI CCAn_nnnn'nTGG
Cuts at: 1497 1497
Size: 4748
BstYI r'GATC_y
Cuts at: 1 1059 1773 2240 2263 2598 2844 3150 3162
Size: 1058 714 467 23 335 246 306 12
Cuts at: 3162 3248 4300 1
Size: 86 1052 449
Fragments arranged by size:
1058 1052 714 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 443 443
Size: 4748
BtgI C'CryG_G
Cuts at: 107 1250 1340 107
Size: 1143 90 3515
Fragments arranged by size:
3515 1143 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2698 2879 3042 2698
Size: 181 163 4404
Fragments arranged by size:
4404 181 163
BtsI GCAGTG_nn'
Cuts at: 1595 1991 1595
Size: 396 4352
ClaI AT'CG_AT
Cuts at: 311 1370 311
Size: 1059 3689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 111 146 1325 1360 111
Size: 35 1179 35 3499
Fragments arranged by size:
3499 1179 35 35
DraI TTT'AAA
Cuts at: 1747 1747
Size: 4748
DraIII CAC_nnn'GTG
Cuts at: 984 1324 1672 984
Size: 340 348 4060
Fragments arranged by size:
4060 348 340
DrdI GACnn_nn'nnGTC
Cuts at: 2454 3798 2454
Size: 1344 3404
EaeI y'GGCC_r
Cuts at: 2333 2507 2898 2925 4270 2333
Size: 174 391 27 1345 2811
Fragments arranged by size:
2811 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2333 2333
Size: 4748
EarI CTCTTCn'nnn_
Cuts at: 1403 1594 2771 2981 1403
Size: 191 1177 210 3170
Fragments arranged by size:
3170 1177 210 191
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2956 3698 3844 4558 2956
Size: 742 146 714 3146
Fragments arranged by size:
3146 742 714 146
EcoICRI GAG'CTC
Cuts at: 348 1760 348
Size: 1412 3336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 401 655 1074 1175 1618 1736 2573 3005
Size: 42 254 419 101 443 118 837 432
Cuts at: 3005 3358 4020 359
Size: 353 662 1087
Fragments arranged by size:
1087 837 662 443 432 419 353 254
118 101 42
EcoNI CCTnn'n_nnAGG
Cuts at: 1261 1261
Size: 4748
EcoO109I rG'GnC_Cy
Cuts at: 1559 1887 1559
Size: 328 4420
EcoRI G'AATT_C
Cuts at: 1752 1752
Size: 4748
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 2767
Size: 4748
FspI TGC'GCA
Cuts at: 2529 2529
Size: 4748
HaeII r_GCGC'y
Cuts at: 198 2182 2430 3660 4431 198
Size: 1984 248 1230 771 515
Fragments arranged by size:
1984 1230 771 515 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 571 603 1318 1350 2590 2620 2622 2652 571
Size: 32 715 32 1240 30 2 30 2667
Fragments arranged by size:
2667 1240 715 32 32 30 30 2
HincII GTy'rAC
Cuts at: 249 258 1337 1787 249
Size: 9 1079 450 3210
Fragments arranged by size:
3210 1079 450 9
HindIII A'AGCT_T
Cuts at: 722 722
Size: 4748
HpaI GTT'AAC
Cuts at: 249 249
Size: 4748
KasI G'GCGC_C
Cuts at: 2178 2426 2178
Size: 248 4500
KpnI G_GTAC'C
Cuts at: 1768 1768
Size: 4748
MluI A'CGCG_T
Cuts at: 4181 4181
Size: 4748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3507 3691 3507
Size: 184 4564
MscI TGG'CCA
Cuts at: 2509 2509
Size: 4748
MslI CAynn'nnrTG
Cuts at: 887 986 1495 2737 2864 887
Size: 99 509 1242 127 2771
Fragments arranged by size:
2771 1242 509 127 99
MspA1I CmG'CkG
Cuts at: 231 553 781 1859 2173 2533 3317 3562 231
Size: 322 228 1078 314 360 784 245 1417
Fragments arranged by size:
1417 1078 784 360 322 314 245 228
NaeI GCC'GGC
Cuts at: 2929 2929
Size: 4748
NarI GG'CG_CC
Cuts at: 2179 2427 2179
Size: 248 4500
NcoI C'CATG_G
Cuts at: 107 1250 1340 107
Size: 1143 90 3515
Fragments arranged by size:
3515 1143 90
NdeI CA'TA_TG
Cuts at: 527 527
Size: 4748
NgoMIV G'CCGG_C
Cuts at: 2927 2927
Size: 4748
NspI r_CATG'y
Cuts at: 478 1104 1801 2301 3904 478
Size: 626 697 500 1603 1322
Fragments arranged by size:
1603 1322 697 626 500
PciI A'CATG_T
Cuts at: 1100 3900 1100
Size: 2800 1948
PflMI CCAn_nnn'nTGG
Cuts at: 1226 1226
Size: 4748
PfoI T'CCnGG_A
Cuts at: 386 1225 386
Size: 839 3909
PmeI GTTT'AAAC
Cuts at: 1747 1747
Size: 4748
PmlI CAC'GTG
Cuts at: 984 984
Size: 4748
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4716
PpuMI rG'GwC_Cy
Cuts at: 1559 1559
Size: 4748
PstI C_TGCA'G
Cuts at: 1280 1795 2480 1280
Size: 515 685 3548
Fragments arranged by size:
3548 685 515
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4748
PvuII CAG'CTG
Cuts at: 231 553 781 2173 2533 231
Size: 322 228 1392 360 2446
Fragments arranged by size:
2446 1392 360 322 228
RsrII CG'GwC_CG
Cuts at: 2943 2943
Size: 4748
SacI G_AGCT'C
Cuts at: 350 1762 350
Size: 1412 3336
SalI G'TCGA_C
Cuts at: 256 1785 256
Size: 1529 3219
SapI GCTCTTCn'nnn_
Cuts at: 2771 2981 2771
Size: 210 4538
SbfI CC_TGCA'GG
Cuts at: 1280 1795 1280
Size: 515 4233
ScaI AGT'ACT
Cuts at: 4265 4265
Size: 4748
SexAI A'CCwGG_T
Cuts at: 1616 1616
Size: 4748
SfcI C'TryA_G
Cuts at: 33 1094 1276 1791 2476 3444 3635 33
Size: 1061 182 515 685 968 191 1146
Fragments arranged by size:
1146 1061 968 685 515 191 182
SfoI GGC'GCC
Cuts at: 2180 2428 2180
Size: 248 4500
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
SmaI CCC'GGG
Cuts at: 1770 1770
Size: 4748
SmlI C'TyrA_G
Cuts at: 970 1903 2818 3532 3794 970
Size: 933 915 714 262 1924
Fragments arranged by size:
1924 933 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4748
SphI G_CATG'C
Cuts at: 478 1801 2301 478
Size: 1323 500 2925
Fragments arranged by size:
2925 1323 500
SspI AAT'ATT
Cuts at: 273 273
Size: 4748
StyI C'CwwG_G
Cuts at: 107 1250 1340 1882 2236 107
Size: 1143 90 542 354 2619
Fragments arranged by size:
2619 1143 542 354 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1229 2331 2464 2923 3132 1229
Size: 1102 133 459 209 2845
Fragments arranged by size:
2845 1102 459 209 133
TatI w'GTAC_w
Cuts at: 746 4263 746
Size: 3517 1231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 482 514 4265 4297 482
Size: 32 3751 32 933
Fragments arranged by size:
3751 933 32 32
Tth111I GACn'n_nGTC
Cuts at: 370 930 2545 370
Size: 560 1615 2573
Fragments arranged by size:
2573 1615 560
XbaI T'CTAG_A
Cuts at: 1779 1779
Size: 4748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1223 1257 2243 1223
Size: 34 986 3728
Fragments arranged by size:
3728 986 34
XmaI C'CCGG_G
Cuts at: 1768 1768
Size: 4748
XmnI GAAnn'nnTTC
Cuts at: 1603 1603
Size: 4748
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I EcoRV FseI FspAI MfeI NheI
NotI NruI NsiI PacI PasI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI