Restriction map for
pF1KB3118
(Circular) (Six-base) MAPSORT of: pF1KB3118.seq Check: 431 from: 1 to: 5657
pF1KB3118 5657 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2695 2695
Size: 5657
Acc65I G'GTAC_C
Cuts at: 2673 2673
Size: 5657
AclI AA'CG_TT
Cuts at: 5497 5497
Size: 5657
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 907 1061 1806 1880 2572 3482 3914 4267
Size: 695 154 745 74 692 910 432 353
Cuts at: 4267 4929 212
Size: 662 940
Fragments arranged by size:
940 910 745 695 692 662 432 353
154 74
AflIII A'CryG_T
Cuts at: 2395 3009 4809 5090 2395
Size: 614 1800 281 2962
Fragments arranged by size:
2962 1800 614 281
AleI CACnn'nnGTG
Cuts at: 2452 2452
Size: 5657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1068 1068
Size: 5657
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2194 2226 2194
Size: 32 5625
AlwNI CAG_nnn'CTG
Cuts at: 471 664 2962 4400 471
Size: 193 2298 1438 1728
Fragments arranged by size:
2298 1728 1438 193
ApaLI G'TGCA_C
Cuts at: 1053 4495 1053
Size: 3442 2215
ApoI r'AATT_y
Cuts at: 420 454 613 819 957 1786 2661 5010 5266
Size: 34 159 206 138 829 875 2349 256
Cuts at: 5266 420
Size: 811
Fragments arranged by size:
2349 875 829 811 256 206 159 138
34
AseI AT'TA_AT
Cuts at: 20 290 20
Size: 270 5387
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5657
AvaI C'yCGr_G
Cuts at: 2677 2677
Size: 5657
AvrII C'CTAG_G
Cuts at: 1743 1743
Size: 5657
BamHI G'GATC_C
Cuts at: 1380 2682 1380
Size: 1302 4355
BanI G'GyrC_C
Cuts at: 2673 3087 3335 3370 2673
Size: 414 248 35 4960
Fragments arranged by size:
4960 414 248 35
BanII G_rGCy'C
Cuts at: 1697 2671 3701 1697
Size: 974 1030 3653
Fragments arranged by size:
3653 1030 974
BbeI G_GCGC'C
Cuts at: 3091 3339 3091
Size: 248 5409
BbsI GAAGACnn'nnnn_
Cuts at: 608 1236 1766 608
Size: 628 530 4499
Fragments arranged by size:
4499 628 530
BbvCI CC'TCA_GC
Cuts at: 2441 2441
Size: 5657
BciVI GTATCCnnnnn_n'
Cuts at: 3555 4611 5657 3555
Size: 1056 1046 3555
Fragments arranged by size:
3555 1056 1046
BclI T'GATC_A
Cuts at: 3177 3177
Size: 5657
BglII A'GATC_T
Cuts at: 3172 5209 3172
Size: 2037 3620
BlpI GC'TnA_GC
Cuts at: 322 2769 322
Size: 2447 3210
Bme1580I G_kGCm'C
Cuts at: 1057 3282 3375 4499 1057
Size: 2225 93 1124 2215
Fragments arranged by size:
2225 2215 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 642 2869 2950 3285 5429 642
Size: 2227 81 335 2144 870
Fragments arranged by size:
2227 2144 870 335 81
BmtI G_CTAG'C
Cuts at: 263 263
Size: 5657
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4024 4024
Size: 5657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 1157 1352 1700 719
Size: 438 195 348 4676
Fragments arranged by size:
4676 438 348 195
Bpu10I CC'TnA_GC
Cuts at: 1065 2441 1065
Size: 1376 4281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1917 2833 3712 4426 4724 1917
Size: 916 879 714 298 2850
Fragments arranged by size:
2850 916 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 221 697 1251 5361 221
Size: 476 554 4110 517
Fragments arranged by size:
4110 554 517 476
BsaAI yAC'GTr
Cuts at: 2454 3010 2454
Size: 556 5101
BsaBI GATnn'nnATC
Cuts at: 6 3171 5208 6
Size: 3165 2037 455
Fragments arranged by size:
3165 2037 455
BsaHI Gr'CG_yC
Cuts at: 3088 3336 4038 5534 3088
Size: 248 702 1496 3211
Fragments arranged by size:
3211 1496 702 248
BsaWI w'CCGG_w
Cuts at: 947 2846 3367 4045 4456 4603 4877 947
Size: 1899 521 678 411 147 274 1727
Fragments arranged by size:
1899 1727 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1325 1355 1325
Size: 30 5627
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2378 2378
Size: 5657
BseYI C'CCAG_C
Cuts at: 186 2404 3082 3840 4505 4913 186
Size: 2218 678 758 665 408 930
Fragments arranged by size:
2218 930 758 678 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1526 1592 184
Size: 1342 66 4249
Fragments arranged by size:
4249 1342 66
BsiEI CG_ry'CG
Cuts at: 104 3199 3245 4475 104
Size: 3095 46 1230 1286
Fragments arranged by size:
3095 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1057 2671 3449 3639 4499 1057
Size: 1614 778 190 860 2215
Fragments arranged by size:
2215 1614 860 778 190
BsmI GAATG_Cn'
Cuts at: 1588 1588
Size: 5657
Bsp1286I G_dGCh'C
Cuts at: 1057 1697 2671 3282 3375 3449 3639 3701 4499
Size: 640 974 611 93 74 190 62 798
Cuts at: 4499 1057
Size: 2215
Fragments arranged by size:
2215 974 798 640 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 947 947
Size: 5657
BspHI T'CATG_A
Cuts at: 4089 4089
Size: 5657
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2707 2921 3223 3604 2707
Size: 214 302 381 4760
Fragments arranged by size:
4760 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3949 4003 5487 5652 50
Size: 3899 54 1484 165 55
Fragments arranged by size:
3899 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 821 3569 821
Size: 2748 2909
BsrFI r'CCGG_y
Cuts at: 159 3655 3836 159
Size: 3496 181 1980
Fragments arranged by size:
3496 1980 181
BsrGI T'GTAC_A
Cuts at: 655 1724 2248 655
Size: 1069 524 4064
Fragments arranged by size:
4064 1069 524
BssSI C'ACGA_G
Cuts at: 1056 3928 4636 1056
Size: 2872 708 2077
Fragments arranged by size:
2872 2077 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3034 3034
Size: 5657
BstBI TT'CG_AA
Cuts at: 4018 4018
Size: 5657
BstEII G'GTnAC_C
Cuts at: 1684 1684
Size: 5657
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2356
Size: 5657
BstYI r'GATC_y
Cuts at: 1 1380 2682 3149 3172 3507 3753 4059 4071
Size: 1379 1302 467 23 335 246 306 12
Cuts at: 4071 4157 5209 1
Size: 86 1052 449
Fragments arranged by size:
1379 1302 1052 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1658 1658
Size: 5657
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3607 3788 3951 3607
Size: 181 163 5313
Fragments arranged by size:
5313 181 163
BtsI GCAGTG_nn'
Cuts at: 2900 2900
Size: 5657
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 875 910 875
Size: 35 5622
DraI TTT'AAA
Cuts at: 2504 2528 2656 2504
Size: 24 128 5505
Fragments arranged by size:
5505 128 24
DrdI GACnn_nn'nnGTC
Cuts at: 3363 4707 3363
Size: 1344 4313
EaeI y'GGCC_r
Cuts at: 3242 3416 3807 3834 5179 3242
Size: 174 391 27 1345 3720
Fragments arranged by size:
3720 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3242 3242
Size: 5657
EarI CTCTTCn'nnn_
Cuts at: 3680 3890 3680
Size: 210 5447
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 168 3865 4607 4753 5467 168
Size: 3697 742 146 714 358
Fragments arranged by size:
3697 742 714 358 146
EcoICRI GAG'CTC
Cuts at: 2669 2669
Size: 5657
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 719 907 1061 1157 1352 1700 1806 1880
Size: 507 188 154 96 195 348 106 74
Cuts at: 1880 2572 3482 3914 4267 4929 212
Size: 692 910 432 353 662 940
Fragments arranged by size:
940 910 692 662 507 432 353 348
195 188 154 106 96 74
EcoO109I rG'GnC_Cy
Cuts at: 2796 2796
Size: 5657
EcoRI G'AATT_C
Cuts at: 454 2661 454
Size: 2207 3450
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 369 1902 3676 369
Size: 1533 1774 2350
Fragments arranged by size:
2350 1774 1533
FspI TGC'GCA
Cuts at: 3438 3438
Size: 5657
HaeII r_GCGC'y
Cuts at: 125 3091 3339 4569 5340 125
Size: 2966 248 1230 771 442
Fragments arranged by size:
2966 1230 771 442 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 784 816 900 932 1654 1686 2620 2652 3499
Size: 32 84 32 722 32 934 32 847
Cuts at: 3499 3529 3531 3561 784
Size: 30 2 30 2880
Fragments arranged by size:
2880 934 847 722 84 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1818 2696 1818
Size: 878 4779
KasI G'GCGC_C
Cuts at: 3087 3335 3087
Size: 248 5409
KpnI G_GTAC'C
Cuts at: 2677 2677
Size: 5657
MfeI C'AATT_G
Cuts at: 2094 2094
Size: 5657
MluI A'CGCG_T
Cuts at: 5090 5090
Size: 5657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 278 4416 4600 227
Size: 51 4138 184 1284
Fragments arranged by size:
4138 1284 184 51
MscI TGG'CCA
Cuts at: 3418 3418
Size: 5657
MslI CAynn'nnrTG
Cuts at: 575 2452 3646 3773 575
Size: 1877 1194 127 2459
Fragments arranged by size:
2459 1877 1194 127
MspA1I CmG'CkG
Cuts at: 190 1090 2019 2545 2768 3082 3442 4226 4471
Size: 900 929 526 223 314 360 784 245
Cuts at: 4471 190
Size: 1376
Fragments arranged by size:
1376 929 900 784 526 360 314 245
223
NaeI GCC'GGC
Cuts at: 3838 3838
Size: 5657
NarI GG'CG_CC
Cuts at: 3088 3336 3088
Size: 248 5409
NdeI CA'TA_TG
Cuts at: 769 769
Size: 5657
NgoMIV G'CCGG_C
Cuts at: 3836 3836
Size: 5657
NheI G'CTAG_C
Cuts at: 259 259
Size: 5657
NspI r_CATG'y
Cuts at: 1776 2043 2058 2399 2710 3210 4813 1776
Size: 267 15 341 311 500 1603 2620
Fragments arranged by size:
2620 1603 500 341 311 267 15
PasI CC'CwG_GG
Cuts at: 1690 1690
Size: 5657
PciI A'CATG_T
Cuts at: 2395 4809 2395
Size: 2414 3243
PmeI GTTT'AAAC
Cuts at: 2656 2656
Size: 5657
PmlI CAC'GTG
Cuts at: 2454 2454
Size: 5657
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 4103
Size: 32 5625
PshAI GACnn'nnGTC
Cuts at: 706 1192 1858 706
Size: 486 666 4505
Fragments arranged by size:
4505 666 486
PsiI TTA'TAA
Cuts at: 716 1220 716
Size: 504 5153
PstI C_TGCA'G
Cuts at: 2704 3389 2704
Size: 685 4972
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5657
PvuII CAG'CTG
Cuts at: 190 1090 2019 2545 3082 3442 190
Size: 900 929 526 537 360 2405
Fragments arranged by size:
2405 929 900 537 526 360
RsrII CG'GwC_CG
Cuts at: 3852 3852
Size: 5657
SacI G_AGCT'C
Cuts at: 2671 2671
Size: 5657
SalI G'TCGA_C
Cuts at: 2694 2694
Size: 5657
SapI GCTCTTCn'nnn_
Cuts at: 3680 3890 3680
Size: 210 5447
SbfI CC_TGCA'GG
Cuts at: 2704 2704
Size: 5657
ScaI AGT'ACT
Cuts at: 1530 1539 2480 5174 1530
Size: 9 941 2694 2013
Fragments arranged by size:
2694 2013 941 9
SfcI C'TryA_G
Cuts at: 33 408 497 2700 3385 4353 4544 33
Size: 375 89 2203 685 968 191 1146
Fragments arranged by size:
2203 1146 968 685 375 191 89
SfoI GGC'GCC
Cuts at: 3089 3337 3089
Size: 248 5409
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5657
SmaI CCC'GGG
Cuts at: 2679 2679
Size: 5657
SmlI C'TyrA_G
Cuts at: 1932 2812 3727 4441 4703 1932
Size: 880 915 714 262 2886
Fragments arranged by size:
2886 915 880 714 262
SpeI A'CTAG_T
Cuts at: 67 1411 67
Size: 1344 4313
SphI G_CATG'C
Cuts at: 2710 3210 2710
Size: 500 5157
StuI AGG'CCT
Cuts at: 434 434
Size: 5657
StyI C'CwwG_G
Cuts at: 825 1743 1919 2791 3145 825
Size: 918 176 872 354 3337
Fragments arranged by size:
3337 918 872 354 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 245 620 1703 2464 3240 3373 3832 4041 245
Size: 375 1083 761 776 133 459 209 1861
Fragments arranged by size:
1861 1083 776 761 459 375 209 133
TatI w'GTAC_w
Cuts at: 655 1528 1537 1724 2248 2468 2478 5172 655
Size: 873 9 187 524 220 10 2694 1140
Fragments arranged by size:
2694 1140 873 524 220 187 10 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1325 1357 5174 5206 1325
Size: 32 3817 32 1776
Fragments arranged by size:
3817 1776 32 32
Tth111I GACn'n_nGTC
Cuts at: 3454 3454
Size: 5657
XbaI T'CTAG_A
Cuts at: 1962 2688 1962
Size: 726 4931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2646 3152 2646
Size: 506 5151
XmaI C'CCGG_G
Cuts at: 2677 2677
Size: 5657
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NheI NspI PasI PciI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI ApaI AscI
BaeI BcgI BfrBI BglI BmgBI BsiWI BsmBI BssHII
BstZ17I BtgI ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI NcoI NotI NruI NsiI PacI PflMI
PfoI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI