Restriction map for
pF1KB3125
(Circular) (Six-base) MAPSORT of: pF1KB3125.seq Check: 1818 from: 1 to: 4895
pF1KB3125 4895 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1374 1933 1374
Size: 559 4336
Acc65I G'GTAC_C
Cuts at: 1911 1911
Size: 4895
AclI AA'CG_TT
Cuts at: 4735 4735
Size: 4895
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2720 3152 3505 4167 2720
Size: 432 353 662 3448
Fragments arranged by size:
3448 662 432 353
AflII C'TTAA_G
Cuts at: 796 796
Size: 4895
AflIII A'CryG_T
Cuts at: 743 875 2247 4047 4328 743
Size: 132 1372 1800 281 1310
Fragments arranged by size:
1800 1372 1310 281 132
AleI CACnn'nnGTG
Cuts at: 517 517
Size: 4895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1241 1364 1241
Size: 123 4772
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 322 354 322
Size: 32 4863
AlwNI CAG_nnn'CTG
Cuts at: 385 549 612 1887 2200 3638 385
Size: 164 63 1275 313 1438 1642
Fragments arranged by size:
1642 1438 1275 313 164 63
ApaLI G'TGCA_C
Cuts at: 3733 3733
Size: 4895
ApoI r'AATT_y
Cuts at: 1088 1603 1899 4248 4504 1088
Size: 515 296 2349 256 1479
Fragments arranged by size:
2349 1479 515 296 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4895
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4895
AvaI C'yCGr_G
Cuts at: 592 1915 592
Size: 1323 3572
BamHI G'GATC_C
Cuts at: 1920 1920
Size: 4895
BanI G'GyrC_C
Cuts at: 862 1911 2325 2573 2608 862
Size: 1049 414 248 35 3149
Fragments arranged by size:
3149 1049 414 248 35
BanII G_rGCy'C
Cuts at: 242 581 1909 2939 242
Size: 339 1328 1030 2198
Fragments arranged by size:
2198 1328 1030 339
BbeI G_GCGC'C
Cuts at: 866 2329 2577 866
Size: 1463 248 3184
Fragments arranged by size:
3184 1463 248
BbsI GAAGACnn'nnnn_
Cuts at: 107 107
Size: 4895
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 125 159 343 377 125
Size: 34 184 34 4643
Fragments arranged by size:
4643 184 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1399 2793 3849 4895 1399
Size: 1394 1056 1046 1399
Fragments arranged by size:
1399 1394 1056 1046
BclI T'GATC_A
Cuts at: 2415 2415
Size: 4895
BglII A'GATC_T
Cuts at: 245 411 2410 4447 245
Size: 166 1999 2037 693
Fragments arranged by size:
2037 1999 693 166
BlpI GC'TnA_GC
Cuts at: 2007 2007
Size: 4895
Bme1580I G_kGCm'C
Cuts at: 1525 2520 2613 3737 1525
Size: 995 93 1124 2683
Fragments arranged by size:
2683 1124 995 93
BmrI ACTGGGnnnn_n'
Cuts at: 244 1830 1885 2107 2188 2523 4667 244
Size: 1586 55 222 81 335 2144 472
Fragments arranged by size:
2144 1586 472 335 222 81 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3262 3262
Size: 4895
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 731 622
Size: 109 4786
Bpu10I CC'TnA_GC
Cuts at: 1631 1631
Size: 4895
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 2071 2950 3664 3962 226
Size: 1845 879 714 298 1159
Fragments arranged by size:
1845 1159 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4599 4599
Size: 4895
BsaAI yAC'GTr
Cuts at: 1482 2248 1482
Size: 766 4129
BsaBI GATnn'nnATC
Cuts at: 6 1813 2409 4446 6
Size: 1807 596 2037 455
Fragments arranged by size:
2037 1807 596 455
BsaHI Gr'CG_yC
Cuts at: 112 863 2326 2574 3276 4772 112
Size: 751 1463 248 702 1496 235
Fragments arranged by size:
1496 1463 751 702 248 235
BsaWI w'CCGG_w
Cuts at: 1805 2084 2605 3283 3694 3841 4115 1805
Size: 279 521 678 411 147 274 2585
Fragments arranged by size:
2585 678 521 411 279 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 664 694 664
Size: 30 4865
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 132 451 469 1876 132
Size: 319 18 1407 3151
Fragments arranged by size:
3151 1407 319 18
BseYI C'CCAG_C
Cuts at: 997 1824 2320 3078 3743 4151 997
Size: 827 496 758 665 408 1741
Fragments arranged by size:
1741 827 758 665 496 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 1421 989
Size: 432 4463
BsiEI CG_ry'CG
Cuts at: 104 357 2437 2483 3713 104
Size: 253 2080 46 1230 1286
Fragments arranged by size:
2080 1286 1230 253 46
BsiHKAI G_wGCw'C
Cuts at: 581 1348 1637 1909 2687 2877 3737 581
Size: 767 289 272 778 190 860 1739
Fragments arranged by size:
1739 860 778 767 289 272 190
BsiWI C'GTAC_G
Cuts at: 191 191
Size: 4895
Bsp1286I G_dGCh'C
Cuts at: 242 581 1348 1525 1637 1909 2520 2613 2687
Size: 339 767 177 112 272 611 93 74
Cuts at: 2687 2877 2939 3737 242
Size: 190 62 798 1400
Fragments arranged by size:
1400 798 767 611 339 272 190 177
112 93 74 62
BspEI T'CCGG_A
Cuts at: 1805 1805
Size: 4895
BspHI T'CATG_A
Cuts at: 3327 3327
Size: 4895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 185 341 1945 2159 2461 2842 185
Size: 156 1604 214 302 381 2238
Fragments arranged by size:
2238 1604 381 302 214 156
BsrBI CCG'CTC
Cuts at: 50 157 3187 3241 4725 4890 50
Size: 107 3030 54 1484 165 55
Fragments arranged by size:
3030 1484 165 107 55 54
BsrDI GCAATG_nn'
Cuts at: 1053 2807 1053
Size: 1754 3141
BsrFI r'CCGG_y
Cuts at: 2893 3074 2893
Size: 181 4714
BsrGI T'GTAC_A
Cuts at: 878 878
Size: 4895
BssSI C'ACGA_G
Cuts at: 3166 3874 3166
Size: 708 4187
BstAPI GCAn_nnn'nTGC
Cuts at: 447 2272 447
Size: 1825 3070
BstBI TT'CG_AA
Cuts at: 3256 3256
Size: 4895
BstXI CCAn_nnnn'nTGG
Cuts at: 234 276 522 690 234
Size: 42 246 168 4439
Fragments arranged by size:
4439 246 168 42
BstYI r'GATC_y
Cuts at: 1 245 411 608 1884 1920 2387 2410 2745
Size: 244 166 197 1276 36 467 23 335
Cuts at: 2745 2991 3297 3309 3395 4447 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1276 1052 467 449 335 306 246 244
197 166 86 36 23 12
BstZ17I GTA'TAC
Cuts at: 1375 1375
Size: 4895
Bsu36I CC'TnA_GG
Cuts at: 1672 1672
Size: 4895
BtgI C'CryG_G
Cuts at: 107 216 1763 107
Size: 109 1547 3239
Fragments arranged by size:
3239 1547 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2845 3026 3189 2845
Size: 181 163 4551
Fragments arranged by size:
4551 181 163
BtsI GCAGTG_nn'
Cuts at: 1348 2138 1348
Size: 790 4105
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 527 562 527
Size: 35 4860
DraI TTT'AAA
Cuts at: 1894 1894
Size: 4895
DraIII CAC_nnn'GTG
Cuts at: 1719 1719
Size: 4895
DrdI GACnn_nn'nnGTC
Cuts at: 2601 3945 2601
Size: 1344 3551
EaeI y'GGCC_r
Cuts at: 731 1473 2480 2654 3045 3072 4417 731
Size: 742 1007 174 391 27 1345 1209
Fragments arranged by size:
1345 1209 1007 742 391 174 27
EagI C'GGCC_G
Cuts at: 2480 2480
Size: 4895
EarI CTCTTCn'nnn_
Cuts at: 275 280 964 1417 2918 3128 275
Size: 5 684 453 1501 210 2042
Fragments arranged by size:
2042 1501 684 453 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3103 3845 3991 4705 3103
Size: 742 146 714 3293
Fragments arranged by size:
3293 742 714 146
EcoICRI GAG'CTC
Cuts at: 579 1907 579
Size: 1328 3567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 731 2720 3152 3505 4167 622
Size: 109 1989 432 353 662 1350
Fragments arranged by size:
1989 1350 662 432 353 109
EcoNI CCTnn'n_nnAGG
Cuts at: 1858 1858
Size: 4895
EcoO109I rG'GnC_Cy
Cuts at: 2034 2034
Size: 4895
EcoRI G'AATT_C
Cuts at: 1899 1899
Size: 4895
EcoRV GAT'ATC
Cuts at: 1488 1488
Size: 4895
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1815 2914 1815
Size: 1099 3796
FspI TGC'GCA
Cuts at: 2676 2676
Size: 4895
HaeII r_GCGC'y
Cuts at: 866 2329 2577 3807 4578 866
Size: 1463 248 1230 771 1183
Fragments arranged by size:
1463 1230 1183 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 304 336 489 521 837 869 2737 2767 2769
Size: 32 153 32 316 32 1868 30 2
Cuts at: 2769 2799 304
Size: 30 2400
Fragments arranged by size:
2400 1868 316 153 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 618 1934 618
Size: 1316 3579
KasI G'GCGC_C
Cuts at: 862 2325 2573 862
Size: 1463 248 3184
Fragments arranged by size:
3184 1463 248
KpnI G_GTAC'C
Cuts at: 1915 1915
Size: 4895
MluI A'CGCG_T
Cuts at: 4328 4328
Size: 4895
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 798 896 1844 3654 3838 798
Size: 98 948 1810 184 1855
Fragments arranged by size:
1855 1810 948 184 98
MscI TGG'CCA
Cuts at: 2656 2656
Size: 4895
MslI CAynn'nnrTG
Cuts at: 517 1223 2884 3011 517
Size: 706 1661 127 2401
Fragments arranged by size:
2401 1661 706 127
MspA1I CmG'CkG
Cuts at: 1072 2006 2320 2680 3464 3709 1072
Size: 934 314 360 784 245 2258
Fragments arranged by size:
2258 934 784 360 314 245
NaeI GCC'GGC
Cuts at: 3076 3076
Size: 4895
NarI GG'CG_CC
Cuts at: 863 2326 2574 863
Size: 1463 248 3184
Fragments arranged by size:
3184 1463 248
NcoI C'CATG_G
Cuts at: 107 1763 107
Size: 1656 3239
NdeI CA'TA_TG
Cuts at: 753 753
Size: 4895
NgoMIV G'CCGG_C
Cuts at: 3074 3074
Size: 4895
NspI r_CATG'y
Cuts at: 747 879 1222 1948 2448 4051 747
Size: 132 343 726 500 1603 1591
Fragments arranged by size:
1603 1591 726 500 343 132
PciI A'CATG_T
Cuts at: 743 875 4047 743
Size: 132 3172 1591
Fragments arranged by size:
3172 1591 132
PflMI CCAn_nnn'nTGG
Cuts at: 549 1887 549
Size: 1338 3557
PfoI T'CCnGG_A
Cuts at: 458 911 1255 458
Size: 453 344 4098
Fragments arranged by size:
4098 453 344
PmeI GTTT'AAAC
Cuts at: 1894 1894
Size: 4895
PmlI CAC'GTG
Cuts at: 1482 1482
Size: 4895
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 4863
PstI C_TGCA'G
Cuts at: 625 1942 2627 625
Size: 1317 685 2893
Fragments arranged by size:
2893 1317 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4895
PvuII CAG'CTG
Cuts at: 1072 2320 2680 1072
Size: 1248 360 3287
Fragments arranged by size:
3287 1248 360
RsrII CG'GwC_CG
Cuts at: 3090 3090
Size: 4895
SacI G_AGCT'C
Cuts at: 581 1909 581
Size: 1328 3567
SalI G'TCGA_C
Cuts at: 1932 1932
Size: 4895
SapI GCTCTTCn'nnn_
Cuts at: 2918 3128 2918
Size: 210 4685
SbfI CC_TGCA'GG
Cuts at: 1942 1942
Size: 4895
ScaI AGT'ACT
Cuts at: 907 4412 907
Size: 3505 1390
SexAI A'CCwGG_T
Cuts at: 199 199
Size: 4895
SfcI C'TryA_G
Cuts at: 33 621 1938 2623 3591 3782 33
Size: 588 1317 685 968 191 1146
Fragments arranged by size:
1317 1146 968 685 588 191
SfoI GGC'GCC
Cuts at: 864 2327 2575 864
Size: 1463 248 3184
Fragments arranged by size:
3184 1463 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4895
SmaI CCC'GGG
Cuts at: 1917 1917
Size: 4895
SmlI C'TyrA_G
Cuts at: 241 796 2050 2965 3679 3941 241
Size: 555 1254 915 714 262 1195
Fragments arranged by size:
1254 1195 915 714 555 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4895
SphI G_CATG'C
Cuts at: 1948 2448 1948
Size: 500 4395
StyI C'CwwG_G
Cuts at: 107 515 1380 1763 1782 2029 2383 107
Size: 408 865 383 19 247 354 2619
Fragments arranged by size:
2619 865 408 383 354 247 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 551 2478 2611 3070 3279 551
Size: 1927 133 459 209 2167
Fragments arranged by size:
2167 1927 459 209 133
TatI w'GTAC_w
Cuts at: 878 905 4410 878
Size: 27 3505 1363
Fragments arranged by size:
3505 1363 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 580 612 642 674 4412 4444 580
Size: 32 30 32 3738 32 1031
Fragments arranged by size:
3738 1031 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 2692 2692
Size: 4895
XbaI T'CTAG_A
Cuts at: 1926 1926
Size: 4895
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 519 682 2390 519
Size: 163 1708 3024
Fragments arranged by size:
3024 1708 163
XmaI C'CCGG_G
Cuts at: 1915 1915
Size: 4895
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BsmI BsmBI
BssHII BstEII ClaI FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI